Bacillus licheniformis chromosome

ABSTRACT

The present invention relates to an isolated polynucleotide of the complete chromosome of  Bacillus licheniformis . The present invention also relates to isolated genes of the chromosome of  Bacillus licheniformis  which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of  Bacillus licheniformis.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. application Ser. No.12/972,306, filed Dec. 17, 2010, which is a divisional of U.S.application Ser. No. 12/322,974, filed Feb. 9, 2009, now U.S. Pat. No.7,863,032, which is a divisional of U.S. application Ser. No.10/983,128, filed Nov. 5, 2004, now U.S. Pat. No. 7,494,798, whichclaims the benefit of U.S. Provisional Application No. 60/535,988, filedJan. 9, 2004, U.S. Provisional Application No. 60/561,059, filed Apr. 8,2004, and U.S. Provisional Application No. 60/572,403, filed May 18,2004. The content of these applications is hereby incorporated byreference as if it was set forth in full below.

REFERENCE TO A SEQUENCE LISTING

This application contains a Sequence Listing in computer readable form,which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to an isolated polynucleotide moleculecomprising the complete chromosome of Bacillus licheniformis. Thepresent invention also relates to features (genes) of the completechromosomal DNA molecule of Bacillus licheniformis which encodebiologically active substances and to nucleic acid constructs, vectors,and host cells comprising the features as well as methods for producingbiologically active substances encoded by the features and to methods ofusing the isolated features derived from the complete chromosomal DNAmolecule of Bacillus licheniformis.

2. Description of the Related Art

Microbes, which make up most of the earth's biomass, have evolved forsome 3.8 billion years. They are found in virtually every environment,surviving and thriving in extremes of heat, cold, radiation, pressure,salt, acidity, and darkness. Often in these environments, no other formsof life are found and the only nutrients come from inorganic matter. Thediversity and range of their environmental adaptations indicate thatmicrobes long ago “solved” many problems for which scientists are stillactively seeking solutions. The value in determining the complete genomesequence of microbes is that it provides a detailed blueprint for theorganism revealing all of the biochemical pathways, substrates,intermediates, and end products as well as regulatory networks, andevolutionary relationships to other microbes. A complete manifest ofproteins, both structural and catalytic, is encoded as a list offeatures in the DNA molecule comprising the genome, as well as theirlikely cellular location.

Knowledge about the enormous range of microbial capacities has broad andfar-reaching implications for environmental, energy, health, andindustrial applications, such as cleanup of toxic-waste, production ofnovel therapeutic and preventive agents (drugs and vaccines), energygeneration and development of renewable energy sources, production ofchemical catalysts, reagents, and enzymes to improve efficiency ofindustrial processes, management of environmental carbon, nitrogen andnutrient cycling, detection of disease-causing organisms and monitoringof the safety of food and water supplies, use of genetically alteredbacteria as living sensors (biosensors) to detect harmful chemicals insoil, air, or water, and understanding of specialized systems used bymicrobial cells to live in natural environments.

Bacillus licheniformis is a gram positive spore-forming bacterium thatis widely distributed as a saprophytic organism in the environment.Unlike most other bacilli that are predominantly aerobic, Bacilluslicheniformis is a facultative anaerobe which may allow it to grow inadditional ecological niches. This species produces a diverse assortmentof extracellular enzymes that are believed to contribute to the processof nutrient cycling in nature (Claus, D. and Berkeley, R. C. W., 1986,In Bergey's Manual of Systematic Bacteriology, Vol. 2., eds. Sneath, P.H. A. et al., Williams and Wilkins Co., Baltimore, Md., pp. 1105-1139).Certain Bacillus licheniformis isolates are capable of denitrification,however, the relevance of this characteristic to environmentaldenitrification may be small since the species generally persists insoil as endospores (Alexander, M., 1977, Introduction to SoilMicrobiology. John Wiley and Sons, Inc., New York).

There are numerous industrial and agricultural uses for Bacilluslicheniformis and its extracellular products. The species has been usedfor decades in the manufacture of industrial enzymes including severalproteases, α-amylase, penicillinase, pentosanase,cycloglucosyltransferase, β-mannanase, and several pectinolytic enzymes,owing largely to its ability to secrete sizeable amounts of degradativeenzymes. Bacillus licheniformis is also used to produce peptideantibiotics such as bacitracin and proticin, in addition to a number ofspecialty chemicals such as citric add, inosine, inosinic acid, andpoly-γ-glutamic acid. The proteases from Bacillus licheniformis are usedin the detergent industry as well as for dehairing and batting ofleather (Eveleigh, D. E., 1981, Scientific American 245, 155-178).Amylases from Bacillus licheniformis are deployed for the hydrolysis ofstarch, desizing of textiles, and sizing of paper (Erickson, R. J.,1976, In Microbiology, ed. Schlesinger, D. (Am. Soc. Microbiol.,Washington, D.C.), pp. 406-419.). Certain strains of Bacilluslicheniformis have shown efficacy to destroy fungal pathogens affectingmaize, grasses, and vegetable crops (U.S. Pat. No. 5,589,381; U.S. Pat.No. 5,665,354). As an endospore-forming bacterium, the ability of theorganism to survive under unfavorable environmental conditions mayenhance its potential as a natural control agent.

Bacillus licheniformis can be differentiated from other bacilli on thebasis of metabolic and physiological tests (Logan, N. A. and Berkeley,R. C. W., 1981, In The Aerobic Endospore-Forming Bacteria Classificationand Identification, eds. Berkeley, R. C. W. and Goodfellow, M., AcademicPress, Inc., London, pp. 106-140; O'Donnell, A. G., Norris, J. R.,Berkeley, R. C. W., Claus, D., Kanero, T., Logan, N. A., and Nozaki. R.,1980, Internat. J. Systematic Bacteriol. 30: 448-459). However,biochemical and phenotypic characteristics may be ambiguous amongclosely related species. Lapidus et al. (Lapidus, A., Galleron, N.,Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002,FEMS Microbiol. Lett. 209: 23-30) recently constructed a physical map ofthe Bacillus licheniformis chromosome using a PCR approach, andestablished a number of regions of co-linearity where gene content andorganization were ostensibly conserved with the Bacillus subtilischromosome.

It would be advantageous to the art to have available the completeprimary structure of the chromosomal DNA molecule of the Bacilluslicheniformis type strain ATCC 14580. With the complete chromosome datain hand, it should be possible to do comparative genomics and proteomicsstudies that can lead to improved industrial strains as well as to abetter understanding of genome evolution among closely-related bacilliin the subtilis-licheniformis group.

It is an object of the present invention to provide an isolatedpolynucleotide with the sequence of the complete chromosome of Bacilluslicheniformis.

SUMMARY OF THE INVENTION

The present invention relates to an isolated polynucleotide of thecomplete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580having the nucleotide sequence of SEQ ID NO: 1.

The present invention also relates to isolated features (genes) of thecomplete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580encoding biologically active substances, selected from the groupconsisting of:

(a) a gene comprising a nucleotide sequence having at least 60% identitywith any of the polynucleotides of SEQ ID NOs: 2-4198; and

(b) a gene comprising a nucleotide sequence which hybridizes under atleast medium stringency conditions with any of the polynucleotides ofSEQ ID NOs: 2-4198, or a complementary strand thereof.

The present invention also relates to biologically active substancesencoded by the isolated genes, and nucleic acid constructs, vectors, andhost cells comprising the genes.

The present invention also relates to methods for producing suchsubstances having biological activity comprising (a) cultivating arecombinant host cell comprising a nucleic acid construct comprising agene encoding the biologically active substance under conditionssuitable for production of the biologically active substance; and (b)recovering the biologically active substance.

The present invention also relates to methods for monitoringdifferential expression of a plurality of genes in a first bacterialcell relative to expression of the same genes in one or more secondbacterial cells, comprising:

(a) adding a mixture of detection reporter-labeled nucleic acidsisolated from the bacterial cells to a substrate containing an array ofBacillus licheniformis genes selected from the group consisting ofnucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs:2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where thedetection reporter-labeled nucleic acids hybridize to complementarysequences of the Bacillus licheniformis genes on the array, wherein thenucleic acids from the first bacterial cell and the one or more secondbacterial cells are labeled with a first detection reporter and one ormore different second detection reporters, respectively; and

(b) examining the array under conditions wherein the relative expressionof the genes in the bacterial cells is determined by the observeddetection signal of each spot on the array in which (i) the Bacilluslicheniformis genes on the array that hybridize to the nucleic acidsobtained from either the first or the one or more second bacterial cellsproduce a distinct first detection signal or one or more seconddetection signals, respectively, and (ii) the Bacillus licheniformisgenes on the array that hybridize to the nucleic acids obtained fromboth the first and one or more second bacterial produce a distinctcombined detection signal.

The present invention also relates to methods for isolating a geneencoding an enzyme, comprising:

(a) adding a mixture of labeled first nucleic acid probes, isolated froma microbial strain cultured on medium without an inducing substrate, andlabeled second nucleic acid probes, isolated from the microbial straincultured on medium with the inducing substrate, to an array of Bacilluslicheniformis genes selected from the group consisting of nucleotidesSEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, orfragments of SEQ ID NOs: 2-4198, under conditions where the labelednucleic acid probes hybridize to complementary sequences of the Bacilluslicheniformis genes on the array, wherein the first nucleic acid probesare labeled with a first reporter and the second nucleic acid probes arelabeled with a second reporter;

(b) examining the array under conditions wherein the relative expressionof the genes of the microbial strain is determined by the observedhybridization reporter signal of each spot on the array in which (i) theBacillus licheniformis genes on the array that hybridize to the firstnucleic acid probes produce a distinct first hybridization reportersignal or the second nucleic acid probes produce a distinct secondhybridization reporter signal, and (ii) the Bacillus licheniformis geneson the array that hybridize to both the first and second nucleic acidprobes produce a distinct combined hybridization reporter signal; and

(c) isolating a gene from the microbial strain that encodes an enzymethat degrades or converts the substrate.

The present invention also relates to genes isolated by such methods andnucleic acid constructs, vectors, and host cells containing the genes.

DEFINITIONS

Biologically active substance: The term “biologically active substance”is defined herein as any substance which is encoded by a single gene ora series of genes (contiguous or non-contiguous) composing abiosynthetic or metabolic pathway or operon or may be the direct orindirect result of the product of a single gene or products of a seriesof genes of the Bacillus licheniformis chromosome. Such substancesinclude, but are not limited to, biopolymers, metabolites, and cellularstructures and components (e.g., ribosome, flagella, etc.). For purposesof the present invention, biological activity is determined according toprocedures known in the art such as those described by Carpenter andSabatini, 2004, Nature 5: 11-22; Sordie et al., 2003, Proceedings of theNational Academy of Sciences USA 100: 11964-11969; Braun and LaBaer,2003, TRENDS in Biotechnology 21; 383-388; and Kaberdin and McDowall,2003, Genome Research 13: 1961-1965.

In the methods of the present invention, the biopolymer may be anybiopolymer. The term “biopolymer” is defined herein as a chain (orpolymer) of identical, similar, or dissimilar subunits (monomers). Thebiopolymer may be, but is not limited to, a nucleic acid, polyamine,polyol, polypeptide (or polyamide), or polysaccharide.

In a preferred aspect, the biopolymer is a polypeptide. The polypeptidemay be any polypeptide having a biological activity of interest. Theterm “polypeptide” is not meant herein to refer to a specific length ofthe encoded product and, therefore, encompasses peptides, oligopeptides,and proteins. The term “polypeptide” also encompasses naturallyoccurring allelic variations.

In a preferred aspect, the polypeptide is an antibody, antigen,antimicrobial peptide, enzyme, growth factor, hormone, immunodilator,neurotransmitter, receptor, reporter protein, structural protein,transcription factor, and transporter.

In a more preferred aspect, the polypeptide is an oxidoreductase,transferase, hydrolase, lyase, isomerase, or ligase. In a most preferredaspect, the polypeptide is an alpha-glucosidase, aminopeptidase,amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase,cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase,alpha-galactosidase, beta-galactosidase, glucoamylase,glucocerebrosidase, alpha-glucosidase, beta-glucosidase, invertase,laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme,peroxidase, phospholipase, phytase, polyphenoloxidase, proteolyticenzyme, ribonuclease, transglutaminase, urokinase, or xylanase.

In another preferred aspect, the polypeptide is a collagen or gelatin.

In another preferred aspect, the biopolymer is a polysaccharide. Thepolysaccharide may be any polysaccharide, including, but not limited to,a mucopolysaccharide (e.g., heparin and hyaluronic acid) and anitrogen-containing polysaccharide (e.g., chitin). In a more preferredaspect, the polysaccharide is hyaluronic acid (hyaluronan).

In the methods of the present invention, the metabolite may be anymetabolite. The metabolite may be encoded by one or more genes, such asa biosynthetic or metabolic pathway. The term “metabolite” encompassesboth primary and secondary metabolites. Primary metabolites are productsof primary or general metabolism of a cell, which are concerned withenergy metabolism, growth, and structure. Secondary metabolites areproducts of secondary metabolism (see, for example, R. B. Herbert, TheBiosynthesis of Secondary Metabolites, Chapman and Hall, New York,1981).

The primary metabolite may be, but is not limited to, an amino acid,fatty acid, nucleoside, nucleotide, sugar, triglyceride, or vitamin.

The secondary metabolite, may be, but is not limited to, an alkaloid,coumarin, flavonoid, polyketide, quinine, steroid, peptide, or terpene.In a preferred aspect, the secondary metabolite is an antibiotic,antifeedant, attractant, bacteriocide, fungicide, hormone, insecticide,or rodenticide.

isolated biologically active substance: The term “isolated biologicallyactive substance” is defined herein as a substance which is at leastabout 20% pure, preferably at least about 40% pure, more preferablyabout 60% pure, even more preferably about 80% pure, most preferablyabout 90% pure, and even most preferably about 95% pure, as determinedby SDS-PAGE, HPLC, capillary electrophoresis, or any other method usedin the art.

Substantially pure biologically active substance or pure biologicallyactive substance: The term “substantially pure biologically activesubstance” is defined herein as a biologically active substancepreparation which contains at most 10%, preferably at most 8%, morepreferably at most 6%, more preferably at most 5% by weight, morepreferably at most 4%, at most 3%, even more preferably at most 2%, mostpreferably at most 1%, and even most preferably at most 0.5% by weightof other material with which it is natively associated. It is,therefore, preferred that the substantially pure biologically activesubstance is at least 92% pure, preferably at least 94% pure, morepreferably at least 95% pure, more preferably at least 96% pure, morepreferably at least 96% pure, more preferably at least 97% pure, evenmore preferably at least 98% pure, most preferably at least 99%, andeven most preferably at least 99.5% pure by weight of the total materialpresent in the preparation. The term “pure biologically activesubstance” is defined as a biologically active substance preparationwhich contains no other material with which it is natively associated.

The biologically active substances of the present invention arepreferably in a substantially pure form. In particular, it is preferredthat the biologically active substances are in “essentially pure form”,i.e., that the biologically active substance preparation is essentiallyfree of other material with which it is natively associated. This can beaccomplished, for example, by preparing the biologically activesubstance by means of well-known recombinant methods or by classicalpurification methods.

Identity: The relatedness between two amino acid sequences or betweentwo nucleotide sequences is described by the parameter “identity”.

For purposes of the present invention, the degree of identity betweentwo amino acid sequences is determined by the Smith-Waterman Proteinmethod for the Genematcher2, as implemented by Paracel Inc. (Pasadena,Calif.), or the BLASTP method as described by Altschul et al., 1990,Journal of Molecular Biology 215: 403-410.

For purposes of the present invention, the degree of identity betweentwo nucleotide sequences is determined by the Smith Waterman nucleotidemethod for the Genematcher2 or BLASTN for the BlastMachine asimplemented by Paracel inc.

Polypeptide Fragment: The term “polypeptide fragment” is defined hereinas a polypeptide, which retains biological activity, having one or moreamino acids deleted from the amino and/or carboxyl terminus of apolypeptide encoded by any of the genes of the present invention, i.e.,polypeptides of SEQ ID NOs: 4199-8395. Preferably, a fragment containsat least 80%, preferably at least 85%, more preferably at least 90%,even more preferably at least 95%, and most preferably at least 97% ofthe amino acid residues of the mature encoded polypeptide product.

Subsequence: The term “subsequence” is defined herein as apolynucleotide comprising a nucleotide sequence of any of SEQ ID NOs:2-4198 except that one or more nucleotides have been deleted from the 5′and/or 3′ end. Preferably, a subsequence contains at least 80%,preferably at least 85%, more preferably at least 90%, even morepreferably at least 95%, and most preferably at least 97% of thenucleotides of any of the genes of the present invention.

Allelic variant: The term “allelic variant” denotes herein any of two ormore alternative forms of a gene occupying the same chromosomal locus.Allelic variation arises naturally through mutation, and may result inpolymorphism within populations. Gene mutations can be silent (no changein the encoded polypeptide) or may encode polypeptides having alteredamino acid sequences. An allelic variant of a polypeptide is apolypeptide encoded by an allelic variant of a gene.

Substantially pure polynucleotide or pure polynucleotide: The term“substantially pure polynucleotide” as used herein refers to apolynucleotide preparation free of other extraneous or unwantednucleotides and is in a form suitable for use within geneticallyengineered production systems. Thus, a substantially pure polynucleotidecontains at most 10%, preferably at most 8%, more preferably at most 6%,more preferably at most 5%, more preferably at most 4%, more preferablyat most 3%, even more preferably at most 2%, most preferably at most 1%,and even most preferably at most 0.5% by weight of other polynucleotidematerial with which it is natively associated. A substantially purepolynucleotide may, however, include naturally occurring 5′ and 3′untranslated regions, such as promoters and terminators. It is preferredthat the substantially pure polynucleotide is at least 92% pure,preferably at least 94% pure, more preferably at least 95% pure, moirepreferably at least 96% pure, more preferably at least 96% pure, morepreferably at least 97% pure, more preferably at least 98% pure, mostpreferably at least 99%, and even most preferably at least 99.5% pure byweight. The polynucleotides of the present invention are preferably in asubstantially pure form. In particular, it is preferred that thepolynucleotides disclosed herein are in “essentially pure form”, i.e.,that the polynucleotide preparation is essentially free of otherpolynucleotide material with which it is natively associated. The term“pure polynucleotide” is defined as a polynucleotide preparation whichcontains no other material with which it is natively associated.

Nucleic add construct: The term “nucleic acid construct” as used hereinrefers to a nucleic acid molecule, either single- or double-stranded,which is isolated from a naturally occurring gene or which has beenmodified to contain segments of nucleic acids in a manner that would nototherwise exist in nature. The term nucleic acid construct is synonymouswith the term “expression cassette” when the nucleic acid constructcontains the control sequences required for expression of a codingsequence of the present invention.

Control sequence: The term “control sequences” is defined herein toinclude all components, which are necessary or advantageous for theexpression of a biologically active substance of the present invention.Each control sequence may be native or foreign to the polynucleotideencoding the substance. Such control sequences include, but are notlimited to, a leader, propeptide sequence, promoter, signal peptidesequence, and transcription terminator. At a minimum, the controlsequences include a promoter, and transcriptional and translational stopsignals. The control sequences may be provided with linkers for thepurpose of introducing specific restriction sites facilitating ligationof the control sequences with the coding region of the polynucleotideencoding a biologically active substance.

Operably linked: The term “operably linked” as used herein refers to aconfiguration in which a control sequence is placed at an appropriateposition relative to the coding sequence of the DNA sequence, such thatthe control sequence directs the expression of a biologically activesubstance.

Coding sequence: When used herein the term “coding sequence” is intendedto cover a nucleotide sequence, which directly specifies the amino acidsequence of its protein product. The boundaries of the coding sequenceare generally determined by an open reading frame, which usually beginswith the ATG start codon or alternative start codons such as GTG andTTG.

Expression: The term “expression” includes any step involved in theproduction of a biologically active substance including, but not limitedto, transcription, post-transcriptional modification, translation,post-translational modification, and secretion.

Expression vector: The term “expression vector” herein covers a DNAmolecule, linear or circular, that comprises a segment encoding abiologically active substance of the invention, and which is operablylinked to additional segments that provide for its transcription.

Host cell: The term “host cell”, as used herein, includes any cell typewhich is susceptible to transformation, transfection, conjugation,electroporation, etc. with a nucleic acid construct, plasmid, or vector.

DETAILED DESCRIPTION OF THE INVENTION

Bacillus licheniformis Chromosome and Features (Genes) Thereof

The present invention relates to an isolated polynucleotide of thecomplete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580having the nucleotide sequence of SEQ ID NO: 1. Bacillus licheniformisATCC 14580, consists of a circular molecule of 4,222,336 base pairs witha mean G+C content of 46.2%. The chromosome contains 4208 predictedprotein-coding genes (SEQ ID NOs: 2-4198) with an average size of 873bp, 7 rRNA operons, and 72 tRNA genes. The deduced amino acid sequencesof the 4208 predicted protein-coding genes are shown in SEQ ID NOs:4199-8395. SEQ ID NO: 4210 corresponds to SEQ ID NO: 2, SEQ ID NO: 4211corresponds to SEQ ID NO: 3, SEQ ID NO: 4212 corresponds to SEQ ID NO:4, etc. The predicted functions of the 4208 gene products are shown inTable 1.

The Bacillus licheniformis chromosome possesses regions that aremarkedly co-linear with the chromosomes of Bacillus subtilis andBacillus halodurans, and approximately 80% of the predicted genes haveBacillus subtilis orthologues.

The present invention also relates to isolated features (genes) of thecomplete chromosomal DNA molecule of Bacillus licheniformis ATCC 14580encoding biologically active substances, selected from the groupconsisting of:

(a) a gene comprising a nucleotide sequence having at least 60% identitywith any of the nucleotide sequences of SEQ ID NOs: 2-4198; and

(b) a gene comprising a nucleotide sequence which hybridizes under atleast medium stringency conditions with any of the genes of SEQ ID NOs:2-4198, or a complementary strand thereof.

In a first aspect, the present invention relates to isolated genes,which have a degree of identity to the nucleotide sequences of any ofSEQ ID NOs: 2-4198 of at least about 60%, preferably at least about 65%,more preferably at least about 70%, more preferably at least about 75%,more preferably at least about 80%, more preferably at least about 85%,even more preferably at least about 90%, most preferably at least about95%, and even most preferably at least about 97%, which encodebiologically active substances having a particular biological activity(hereinafter “homologous biologically active substances”).

In a second aspect, the present invention relates to isolated genescomprising nucleotide sequences which hybridize under very lowstringency conditions, preferably low stringency conditions, morepreferably medium stringency conditions, more preferably medium-highstringency conditions, even more preferably high stringency conditions,and most preferably very high stringency conditions with any of (i) thegenes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii)complementary strands thereof (J. Sambrook, E. F. Fritsch, and T.Maniatus, 1989, Molecular Cloning, A Laboratory Manual, 2d edition, ColdSpring Harbor, N.Y.). Subsequences of SEQ ID NOs: 2-4198 may be at least100 nucleotides or preferably at least 200 nucleotides. Moreover, thesubsequences may encode fragments of a gene product which havebiological activity. The biologically active substances may also bebiologically active allelic variants of the biologically activesubstances.

The nucleotide sequences of SEQ ID NOs: 2-4198 or subsequences thereof,as well as the amino acid sequences of SEQ ID NOs: 4199-8395 orfragments thereof, may be used to design nucleic acid probes to identifyand clone DNA encoding biologically active substances from strains ofdifferent genera or species according to methods well known in the art.In particular, such probes can be used for hybridization with thegenomic DNA of the genus or species of interest, following standardSouthern blotting procedures, in order to identify and isolate thecorresponding gene therein. Such probes can be considerably shorter thanthe entire sequence, but should be at least 14, preferably at least 25,more preferably at least 35 nucleotides in length, such as at least 70nucleotides in length. It is preferred, however, that the nucleic acidprobes are at least 100 nucleotides in length. For example, the nucleicacid probes may be at least 200 nucleotides, at least 300 nucleotides,at least 400 nucleotides, or at least 500 nucleotides in length. Evenlonger probes may be used, e.g., nucleic acid probes which are at least600 nucleotides, at least 700 nucleotides, at least 800 nucleotides, orat least 900 nucleotides in length. Both DNA and RNA probes can be used.The probes are typically labeled for detecting the corresponding gene(for example, with ³²P, ³H, ³⁵S, biotin, or avidin). Such probes areencompassed by the present invention.

A genomic DNA library prepared from such other organisms may, therefore,be screened for DNA which hybridizes with the probes described above andwhich encodes a biologically active substance. Genomic DNA from suchother organisms may be separated by agarose or polyacrylamide gelelectrophoresis, or other separation techniques. DNA from the librariesor the separated DNA may be transferred to and immobilized onnitrocellulose or other suitable carrier material. In order to identifya clone or DNA which is homologous with any of SEQ ID NOs: 2-4198 orsubsequences thereof, the carrier material is used in a Southern blot.

For purposes of the present invention, hybridization indicates that apolynucleotide hybridizes to a labeled gene having the nucleotidesequence shown in any of SEQ ID NOs: 2-4198, complementary strandsthereof, or subsequences thereof, under very low to very high stringencyconditions. Molecules to which the nucleic acid probe hybridizes underthese conditions can be detected using X-ray film.

In a preferred aspect, the nucleic acid probe is any of the genes of SEQID NOs: 2-4198, or subsequences thereof. In another preferred aspect,the nucleic acid probe is the mature coding region of any of the genesof SEQ ID NOs: 2-4198. In another preferred aspect, the nucleic acidprobe is the gene of any of SEQ ID NOs: 2-4198 contained in Bacilluslicheniformis ATCC 14580, in another preferred aspect, the nucleic acidprobe is the mature coding region of any of the genes of SEQ ID NOs:2-4198 contained in Bacillus licheniformis ATCC 14580.

For long probes of at least 100 nucleotides in length, very low to veryhigh stringency conditions are defined as prehybridization andhybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared anddenatured salmon sperm DNA, and either 25% formamide for very low andlow stringencies, 35% formamide for medium and medium-high stringencies,or 50% formamide for high and very high stringencies, following standardSouthern blotting procedures.

For long probes of at least 100 nucleotides in length, the carriermaterial is finally washed three times each for 15 minutes using 2×SSC,0.2% SDS preferably at least at 45° C. (very low stringency), morepreferably at least at 50° C. (low stringency), more preferably at leastat 55° C. (medium stringency), more preferably at least at 60° C.(medium-high stringency), even more preferably at least at 65° C. (highstringency), and most preferably at least at 70° C. (very highstringency).

For short probes which are about 14 nucleotides to about 70 nucleotidesin length, stringency conditions are defined as prehybridization,hybridization, and washing post-hybridization at about 5° C. to about10° C. below the calculated T_(m) using the calculation according toBolton and McCarthy (1962, Proceedings of the National Academy ofSciences USA 48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA,0.5% NP-40, 1×Denhardt's solution, 1 mM sodium pyrophosphate, 1 mMsodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per mlfollowing standard Southern blotting procedures.

For short probes which are about 14 nucleotides to about 70 nucleotidesin length, the carrier material is washed once in 6×SCC plus 0.1% SDSfor 15 minutes and twice each for 15 minutes using 6×SSC at 5° C. to 10°C. below the calculated T_(m).

Under salt-containing hybridization conditions, the effective T_(m) iswhat controls the degree of identity required between the probe and thefilter bound DNA for successful hybridization. The effective T_(m) maybe determined using the formula below to determine the degree ofidentity required for two DNAs to hybridize under various stringencyconditions.

Effective T_(m)=81.5+16.6(log M[Na⁺])+0.41(%G+C)−0.72(%formamide)

The % G+C content of any of the genes of SEQ ID NOs: 2-4198 can easilybe determined. For medium stringency, for example, the concentration offormamide is 35% and the Na⁺ concentration for 5×SSPE is 0.75 M.Applying this formula to these values, the Effective T_(m) in ° C. canbe calculated. Another relevant relationship is that a 1% mismatch oftwo DNAs lowers the T_(m) 1.4° C. To determine the degree of identityrequired for two DNAs to hybridize under medium stringency conditions at42° C., the following formula is used:

%Homology=100−[(Effective T_(m)−Hybridization Temperature)/1.4]

Applying this formula, the degree of identity required for two DNAs tohybridize under medium stringency conditions at 42° C. can becalculated.

Similar calculations can be made under other stringency conditions, asdefined herein.

The present invention also relates to isolated polynucleotides obtainedby (a) hybridizing a population of DNA under very low, low, medium,medium-high, high, or very high stringency conditions with any of (i)the genes of SEQ ID NOs: 2-4198, or subsequences thereof, or (ii)complementary strands thereof; and (b) isolating the hybridizingpolynucleotide from the population of DNA. In a preferred aspect, thehybridizing polynucleotide encodes a polypeptide of any of SEQ ID NOs:2-4198, or homologous polypeptides thereof.

In a third aspect, the present invention relates to isolatedpolypeptides having amino acid sequences which have a degree of identityto any of SEQ ID NOs: 4199-8395 of at least about 60%, preferably atleast about 65%, more preferably at least about 70%, more preferably atleast about 75%, more preferably at least about 80%, more preferably atleast about 85%, even more preferably at least about 90%, mostpreferably at least about 95%, and even most preferably at least about97%, which have biological activity (hereinafter “homologouspolypeptides”). In a preferred aspect, the homologous polypeptides havean amino acid sequence which differs by ten amino acids, preferably byfive amino acids, more preferably by four amino acids, even morepreferably by three amino acids, most preferably by two amino acids, andeven most preferably by one amino acid from the amino acid sequences ofSEQ ID NOs: 4199-8395.

The polypeptides of the present invention preferably comprise the aminoacid sequence of any of SEQ ID NOs: 4199-8395 or an allelic variantthereof; or a fragment thereof that has biological activity. In a morepreferred aspect, the polypeptides of the present invention comprise theamino acid sequence of any of SEQ ID NOs: 4199-8395. In anotherpreferred aspect, the polypeptides of the present invention comprise themature polypeptide region of any of SEQ ID NOs: 4199-8395, or an allelicvariant thereof; or a fragment thereof that has biological activity. Inanother preferred aspect, the polypeptides of the present inventioncomprise the mature polypeptide region of any of SEQ ID NOs: 4199-8395.In another preferred aspect, the polypeptides of the present inventionconsist of the amino acid sequence of any of SEQ ID NOs: 4199-8395 or anallelic variant thereof; or a fragment thereof that has biologicalactivity. In another preferred aspect, the polypeptides of the presentinvention consist of the amino acid sequence of any of SEQ ID NOs:4199-8395. In another preferred aspect, the polypeptides consist of themature polypeptide region of any of SEQ ID NOs: 4199-8395 or an allelicvariant thereof; or a fragment thereof that has biological activity. Inanother preferred aspect, the polypeptides consist of the maturepolypeptide region of any of SEQ ID NOs: 4199-8395.

In a fourth aspect, the present invention relates to isolated substanceshaving biological activity which are encoded by polynucleotides whichhybridize, as described above, under very low stringency conditions,preferably low stringency conditions, more preferably medium stringencyconditions, more preferably medium-high stringency conditions, even morepreferably high stringency conditions, and most preferably very highstringency conditions with which hybridize under very low stringencyconditions, preferably low stringency conditions, more preferably mediumstringency conditions, more preferably medium-high stringencyconditions, even more preferably high stringency conditions, and mostpreferably very high stringency conditions with any of (i) the genes ofSEQ ID NOs: 2-4198, or subsequences thereof, or (ii) complementarystrands thereof. A subsequence of any of SEQ ID NOs: 2-4198 may be atleast 100 nucleotides or preferably at least 200 nucleotides. Moreover,the subsequence may encode a fragment, e.g., a polypeptide fragment,which has biological activity.

Nucleic Acid Constructs

The present invention also relates to nucleic acid constructs comprisingan isolated gene or isolated genes (e.g., operon) of the presentinvention operably linked to one or more control sequences which directthe expression of the coding sequence in a suitable host cell underconditions compatible with the control sequences.

An isolated gene(s) of the present invention may be manipulated in avariety of ways to provide for production of a biologically activesubstance encoded directly or indirectly by the gene(s). Manipulation ofthe nucleotide sequence prior to its insertion into a vector may bedesirable or necessary depending on the expression vector. Thetechniques for modifying nucleotide sequences utilizing recombinant DNAmethods are well known in the art.

The control sequence may be an appropriate promoter sequence, anucleotide sequence which is recognized by a host cell for expression ofthe gene(s) encoding the biologically active substance. The promotersequence contains transcriptional control sequences which mediate theexpression of the biologically active substance. The promoter may be anynucleotide sequence which shows transcriptional activity in the hostcell of choice including mutant, truncated, and hybrid promoters, andmay be obtained from genes encoding extracellular or intracellularpolypeptides or biologically active substances either homologous orheterologous to the host cell.

Examples of suitable promoters for directing the transcription of thenucleic acid constructs of the present invention, especially in abacterial host cell, are the promoters obtained from the E. coli lacoperon, Streptomyces coelicolor agarase gene (dagA), Bacillus subtilislevansucrase gene (sacB), Bacillus licheniformis alpha-amylase gene(amyL), Bacillus stearothermophilus maltogenic amylase gene (amyM),Bacillus amyloliquefaciens alpha-amylase gene (amyQ), Bacilluslicheniformis penicillinase gene (penP), Bacillus subtilis xylA and xylBgenes, and prokaryotic beta-lactamase gene (Villa-Kamaroff et al., 1978,Proceedings of the National Academy of Sciences USA 75: 3727-3731), aswell as the tac promoter (DeBoer et al., 1983, Proceedings of theNational Academy of Sciences USA 80: 21-25). Further promoters aredescribed in “Useful proteins from recombinant bacteria” in ScientificAmerican, 1980, 242: 74-94; and in Sambrook et al., 1989, supra.

The control sequence may also be a suitable transcription terminatorsequence, a sequence recognized by a host cell to terminatetranscription. The terminator sequence is operably linked to the 3′terminus of the gene encoding the biologically active substance. Anyterminator which is functional in the host cell of choice may be used inthe present invention.

The control sequence may also be a signal peptide coding region thatcodes for an amino acid sequence linked to the amino terminus of apolypeptide and directs the encoded polypeptide into the cell'ssecretory pathway. The 5′ end of the coding sequence of the nucleotidesequence may inherently contain a signal peptide coding region naturallylinked in translation reading frame with the segment of the codingregion which encodes the secreted polypeptide. Alternatively, the 5′ endof the coding sequence may contain a signal peptide coding region whichis foreign to the coding sequence. The foreign signal peptide codingregion may be required where the coding sequence does not naturallycontain a signal peptide coding region. Alternatively, the foreignsignal peptide coding region may simply replace the natural signalpeptide coding region in order to enhance secretion of the polypeptide.However, any signal peptide coding region which directs the expressedpolypeptide into the secretory pathway of a host cell of choice may beused in the present invention.

Effective signal peptide coding regions for bacterial host cells are thesignal peptide coding regions obtained from the genes for Bacillus NCIB11837 maltogenic amylase, Bacillus stearothermophilus alpha-amylase,Bacillus licheniformis subtilisin, Bacillus licheniformisbeta-lactamase, Bacillus stearothermophilus neutral proteases (nprT,nprS, nprM), and Bacillus subtilis prsA. Further signal peptides aredescribed by Simonen and Palva, 1993, Microbiological Reviews 57:109-137.

The control sequence may also be a propeptide coding region that codesfor an amino acid sequence positioned at the amino terminus of apolypeptide. The resultant polypeptide is known as a proenzyme orpropolypeptide (or a zymogen in some cases). A propolypeptide isgenerally inactive and can be converted to a mature active polypeptideby catalytic or autocatalytic cleavage of the propeptide from thepropolypeptide. The propeptide coding region may be obtained from thegenes for Bacillus subtilis alkaline protease (aprE) and Bacillussubtilis neutral protease (nprT).

Where both signal peptide and propeptide regions are present at theamino terminus of a polypeptide, the propeptide region is positionednext to the amino terminus of a polypeptide and the signal peptideregion is positioned next to the amino terminus of the propeptideregion.

It may also be desirable to add regulatory sequences which allow theregulation of the expression of a biologically active substance relativeto the growth of the host cell. Examples of regulatory systems are thosewhich cause the expression of the gene to be turned on or off inresponse to a chemical or physical stimulus, including the presence of aregulatory compound. Regulatory systems in prokaryotic systems includethe lac, lac, and trp operator systems. Other examples of regulatorysequences are those which allow for gene amplification. In eukaryoticsystems, these include the dihydrofolate reductase gene which isamplified in the presence of methotrexate, and the metallothionein geneswhich are amplified with heavy metals. In these cases, the nucleotidesequence encoding the biologically active substance would be operablylinked with the regulatory sequence.

Expression Vectors

The present invention also relates to recombinant expression vectorscomprising an isolated gene of the present invention, a promoter, andtranscriptional and translational stop signals. The various nucleic acidand control sequences described above may be joined together to producea recombinant expression vector which may include one or more convenientrestriction sites to allow for insertion or substitution of thenucleotide sequence encoding the polypeptide at such sites.Alternatively, a gene of the present invention may be expressed byinserting the nucleotide sequence or a nucleic acid construct comprisingthe sequence into an appropriate vector for expression. In creating theexpression vector, the coding sequence is located in the vector so thatthe coding sequence is operably linked with the appropriate controlsequences for expression.

The recombinant expression vector may be any vector (e.g., a plasmid orvirus) which can be conveniently subjected to recombinant DNA proceduresand can bring about the expression of a gene of the present invention.The choice of the vector will typically depend on the compatibility ofthe vector with the host cell into which the vector is to be introduced.The vectors may be linear or closed circular plasmids.

The vector may be an autonomously replicating vector, i.e., a vectorwhich exists as an extrachromosomal entity, the replication of which isindependent of chromosomal replication, e.g., a plasmid, anextrachromosomal element, a minichromosome, or an artificial chromosome.The vector may contain any means for assuring self-replication.Alternatively, the vector may be one which, when introduced into thehost cell, is integrated into the genome and replicated together withthe chromosome(s) into which it has been integrated. Furthermore, asingle vector or plasmid or two or more vectors or plasmids whichtogether contain the total DNA to be introduced into the genome of thehost cell, or a transposon may be used.

The vectors of the present invention preferably contain one or moreselectable markers which permit easy selection of transformed cells. Aselectable marker is a gene the product of which provides for biocide orviral resistance, resistance to heavy metals, prototrophy to auxotrophs,and the like.

Examples of bacterial selectable markers are the dal genes from Bacillussubtilis or Bacillus licheniformis, or markers which confer antibioticresistance such as ampicillin, kanamycin, chloramphenicol ortetracycline resistance.

The vectors of the present invention preferably contain an element(s)that permits integration of the vector into the host cell's genome orautonomous replication of the vector in the cell independent of thegenome.

For integration into the host cell genome, the vector may rely onportions of the sequence of the gene or any other element of the vectorfor integration of the vector into the genome by homologous ornonhomologous recombination. Alternatively, the vector may containadditional nucleotide sequences for directing integration by homologousrecombination into the genome of the host cell. The additionalnucleotide sequences enable the vector to be integrated into the hostcell genome at a precise location(s) in the chromosome(s). To increasethe likelihood of integration at a precise location, the integrationalelements should preferably contain a sufficient number of nucleotides,such as 100 to 10,000 base pairs, preferably 400 to 10,000 base pairs,and most preferably 800 to 10,000 base pairs, which are highlyhomologous with the corresponding target sequence to enhance theprobability of homologous recombination. The integrational elements maybe any sequence that is homologous with the target sequence in thegenome of the host cell. Furthermore, the integrational elements may benon-encoding or encoding nucleotide sequences. On the other hand, thevector may be integrated into the genome of the host cell bynon-homologous recombination.

For autonomous replication, the vector may further comprise an origin ofreplication enabling the vector to replicate autonomously in the hostcell in question. The origin of replication may be any plasmidreplicator mediating autonomous replication which functions in a cell.The term “origin of replication” or “plasmid replicator” is definedherein as a sequence that enables a plasmid or vector to replicate invivo. Examples of bacterial origins of replication are the origins ofreplication of plasmids pBR322, pUC19, pACYC177, and pACYC184 permittingreplication in E. coli, and pUB110, pE194, pTA1060, and pAMβ1 permittingreplication in Bacillus.

More than one copy of a gene of the present invention may be insertedinto the host cell to increase production of the gene product. Anincrease in the copy number of the gene can be obtained by integratingat least one additional copy of the sequence into the host cell genomeor by including an amplifiable selectable marker gene with a gene of thepresent invention where cells containing amplified copies of theselectable marker gene, and thereby additional copies of the gene of thepresent invention, can be selected for by cultivating the cells in thepresence of the appropriate selectable agent.

The procedures used to ligate the elements described above to constructthe recombinant expression vectors of the present invention are wellknown to one skilled in the art (see, e.g. Sambrook et al., 1989,supra).

Host Cells

The present invention also relates to recombinant host cells, comprisingan isolated gene of the present invention, where the host cells areadvantageously used in the recombinant production of a biologicallyactive substance encoded by the gene. A vector comprising a gene of thepresent invention is introduced into a host cell so that the vector ismaintained as a chromosomal integrant or as a self-replicatingextra-chromosomal vector as described earlier. The term “host cell”encompasses any progeny of a parent cell that is not identical to theparent cell due to mutations that occur during replication. The choiceof a host cell will to a large extent depend upon the gene encoding thebiologically active substance and its source.

The host cell may be any unicellular microorganism, e.g., a prokaryote.

Useful unicellular cells are bacterial cells such as gram positivebacteria including, but not limited to, a Bacillus cell, e.g., Bacillusalkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacilluscereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans,Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacilluslentils, Bacillus licheniformis, Bacillus macerans, Bacillus megaterium,Bacillus methanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillusstearothermophilus, Bacillus subtilis, and Bacillus thuringiensis; or aStreptomyces e.g., Streptomyces lividans and Streptomyces murinus, orgram negative bacteria such as E. coli and Pseudomonas sp. In apreferred aspect, the bacterial host cell is a Bacillus lentus, Bacilluslicheniformis, Bacillus stearothermophilus, or Bacillus subtilis cell.In another preferred aspect, the Bacillus cell is an alkalophilicBacillus.

The introduction of a vector into a bacterial host cell may, forinstance, be effected by protoplast transformation (see, e.g., Chang andCohen, 1979, Molecular General Genetics 168: 111-115), using competentcells (see, e.g., Young and Spizizen, 1961, Journal of Bacteriology 81:823-829, or Dubnau and Davidoff-Abelson, 1971, Journal of MolecularBiology 56: 209-221), electroporation (see, e.g., Shigekawa and Dower,1988, Biotechniques 6: 742-751), or conjugation (see, e.g., Koehler andThorne, 1987, Journal of Bacteriology 169: 5771-5278).

Methods of Production

The present invention also relates to methods for producing abiologically active substance of the present invention comprising (a)cultivating a strain, which in its wild-type form is capable ofproducing the biologically active substance, under conditions conducivefor production of the biologically active substance; and (b) recoveringthe biologically active substance. Preferably, the strain is of thegenus Bacillus, and more preferably Bacillus licheniformis.

The present invention also relates to methods for producing abiologically active substance of the present invention comprising (a)cultivating a host cell under conditions conducive for production of thebiologically active substance; and (b) recovering the biologicallyactive substance.

The present invention also relates to methods for producing abiologically active substance of the present invention comprising (a)cultivating a host cell under conditions conducive for production of thebiologically active substance, wherein the host cell comprises a mutantpolynucleotide comprising at least one mutation in the coding region ofany of SEQ ID NOs: 2-4198, wherein the mutant polynucleotide encodes abiologically active substance which consists of SEQ ID NOs: 4199-8395,respectively, and (b) recovering the biologically active substance.

In the production methods of the present invention, the cells arecultivated in a nutrient medium suitable for production of thebiologically active substance using methods known in the art. Forexample, the cell may be cultivated by shake flask cultivation, andsmall-scale or large-scale fermentation (including continuous, batch,fed-batch, or solid state fermentations) in laboratory or industrialfermentors performed in a suitable medium and under conditions allowingthe biologically active substance to be expressed and/or isolated. Thecultivation takes place in a suitable nutrient medium comprising carbonand nitrogen sources and inorganic salts, using procedures known in theart. Suitable media are available from commercial suppliers or may beprepared according to published compositions (e.g., in catalogues of theAmerican Type Culture Collection). If the biologically active substanceis secreted into the nutrient medium, the biologically active substancecan be recovered directly from the medium. If the biologically activesubstance is not secreted, it can be recovered from cell lysates.

The biologically active substances may be detected using methods knownin the art that are specific for the polypeptides. These detectionmethods may include use of specific antibodies, formation of an enzymeproduct, or disappearance of an enzyme substrate. For example, an enzymeassay may be used to determine the activity of an enzyme.

The resulting biologically active substances may be recovered by methodsknown in the art. For example, the biologically active substances may berecovered from the nutrient medium by conventional procedures including,but not limited to, centrifugation, filtration, extraction,spray-drying, evaporation, or precipitation.

The biologically active substances of the present invention may bepurified by a variety of procedures known in the art including, but notlimited to, chromatography (e.g., ion exchange, affinity, hydrophobic,chromatofocusing, and size exclusion), electrophoretic procedures (e.g.,preparative isoelectric focusing), differential solubility (e.g.,ammonium sulfate precipitation), SDS-PAGE, or extraction (see, e.g.,Protein Purification, J.-C. Janson and Lars Ryden, editors, VCHPublishers, New York, 1989).

Plants

The present invention also relates to a transgenic plant, plant part, orplant cell which has been transformed with a gene encoding abiologically active substance of the present invention so as to expressand produce the biologically active substance in recoverable quantities.The biologically active substance may be recovered from the plant orplant part. Alternatively, the plant or plant part containing therecombinant biologically active substance may be used as such forimproving the quality of a food or feed, e.g., improving nutritionalvalue, palatability, and rheological properties, or to destroy anantinutritive factor.

The transgenic plant can be dicotyledonous (a dicot) or monocotyledonous(a monocot). Examples of monocot plants are grasses, such as meadowgrass (blue grass, Poa), forage grass such as festuca, lolium, temperategrass, such as Agrostis, and cereals, e.g., wheat, oats, rye, barley,rice, sorghum, and maize (corn).

Examples of dicot plants are tobacco, legumes, such as lupins, potato,sugar beet, pea, bean and soybean, and cruciferous plants (familyBrassicaceae), such as cauliflower, rape seed, and the closely relatedmodel organism Arabidopsis thaliana.

Examples of plant parts are stem, callus, leaves, root, fruits, seeds,and tubers as well as the individual tissues comprising these parts,e.g., epidermis, mesophyll, parenchyme, vascular tissues, meristems. Inthe present context, also specific plant cell compartments, such aschloroplast, apoplast, mitochondria, vacuole, peroxisomes and cytoplasmare considered to be a plant part. Furthermore, any plant cell, whateverthe tissue origin, is considered to be a plant part. Likewise, plantparts such as specific tissues and cells isolated to facilitate theutilisation of the invention are also considered plant parts e.g.embryos, endosperms, aleurone and seeds coats.

Also included within the scope of the present invention are the progenyof such plants, plant parts, and plant cells.

The transgenic plant or plant cell expressing a biologically activesubstance of the present invention may be constructed in accordance withmethods known in the art. Briefly, the plant or plant cell isconstructed by incorporating one or more expression constructs encodinga biologically active substance of the present invention into the planthost genome and propagating the resulting modified plant or plant cellinto a transgenic plant or plant cell.

The expression construct is conveniently a nucleic acid construct whichcomprises a gene encoding a biologically active substance of the presentinvention operably linked with appropriate regulatory sequences requiredfor expression of the nucleotide sequence in the plant or plant part ofchoice. Furthermore, the expression construct may comprise a selectablemarker useful for identifying host cells into which the expressionconstruct has been integrated and DNA sequences necessary forintroduction of the construct into the plant in question (the latterdepends on the DNA introduction method to be used).

The choice of regulatory sequences, such as promoter and terminatorsequences and optionally signal or transit sequences is determined, forexample, on the basis of when, where, and how the biologically activesubstance is desired to be expressed. For instance, the expression ofthe gene encoding a biologically active substance of the presentinvention may be constitutive or inducible, or may be developmental,stage or tissue specific, and the gene product may be targeted to aspecific tissue or plant part such as seeds or leaves. Regulatorysequences are, for example, described by Tague et al., 1988, PlantPhysiology 86: 506.

For constitutive expression the 35S-CaMV, the maize ubiquitin 1 and therice actin 1 promoter may be used (Franck et al., 1980. Cell 21:285-294, Christensen A H, Sharrock R A and Quail, 1992, Plant Mo. Biol.18: 675-689; Zhang W, McElroy D. and Wu R., 1991, Plant Cell 3:1155-1165). Organ-specific promoters may be, for example, a promoterfrom storage sink tissues such as seeds, potato tubers, and fruits(Edwards and Coruzzi, 1990, Ann. Rev. Genet. 24: 275-303), or frommetabolic sink tissues such as meristems (Ito et al., 1994, Plant Mol.Biol. 24: 863-878), a seed specific promoter such as the glutelin,prolamin, globulin, or albumin promoter from rice (Wu et al., 1998,Plant and Cell Physiology 39: 885-889), a Vicia faba promoter from thelegumin B4 and the unknown seed protein gene from Vicia faba (Conrad etal., 1998, Journal of Plant Physiology 152: 708-711), a promoter from aseed oil body protein (Chen et al., 1998, Plant and Cell Physiology 39:935-941), the storage protein napA promoter from Brassica napus, or anyother seed specific promoter known in the art, e.g., as described in WO91/14772. Furthermore, the promoter may be a leaf specific promoter suchas the rbcs promoter from rice or tomato (Kyozuka et al., 1993, PlantPhysiology 102: 991-1000, the chlorella virus adenine methyltransferasegene promoter (Mitra and Higgins, 1994, Plant Molecular Biology 26:85-93), or the aldP gene promoter from rice (Kagaya et al., 1995,Molecular and General Genetics 248: 668-674), or a wound induciblepromoter such as the potato pint promoter (Xu et al., 1993, PlantMolecular Biology 22: 573-588). Likewise, the promoter may inducible byabiotic treatments such as temperature, drought or alterations insalinity or induced by exogenously applied substances that activate thepromoter, e.g., ethanol, oestrogens, plant hormones like ethylene,abscisic acid and gibberellic acid and heavy metals.

A promoter enhancer element may also be used to achieve higherexpression of the enzyme in the plant. For instance, the promoterenhancer element may be an intron which is placed between the promoterand the nucleotide sequence encoding a biologically active substance ofthe present invention. For instance, Xu et al., 1993, supra disclose theuse of the first intron of the rice actin 1 gene to enhance expression.

The selectable marker gene and any other parts of the expressionconstruct may be chosen from those available in the art.

The nucleic acid construct is incorporated into the plant genomeaccording to conventional techniques known in the art, includingAgrobacterium-mediated transformation, virus-mediated transformation,microinjection, particle bombardment, biolistic transformation, andelectroporation (Gasser et al., 1990, Science 244: 1293; Potrykus, 1990,Bio/Technology 8: 535; Shimamoto et al., 1989, Nature 338: 274).

Presently, Agrobacterium tumefaciens-mediated gene transfer is themethod of choice for generating transgenic dicots (for a review, seeHooykas and Schilperoort, 1992, Plant Molecular Biology 19: 15-38).However it can also be used for transforming monocots, although othertransformation methods are generally preferred for these plants.Presently, the method of choice for generating transgenic monocots isparticle bombardment (microscopic gold or tungsten particles coated withthe transforming DNA) of embryonic calli or developing embryos(Christou, 1992, Plant Journal 2: 275-281; Shimamoto, 1994, CurrentOpinion Biotechnology 5: 158-162: Vasil et al., 1992. Bio/Technology 10:667-674). An alternative method for transformation of monocots is basedon protoplast transformation as described by Omirulleh et al., 1993,Plant Molecular Biology 21: 415-428.

Following transformation, the transformants having incorporated thereinthe expression construct are selected and regenerated into whole plantsaccording to methods well-known in the art. Often the transformationprocedure is designed for the selective elimination of selection geneseither during regeneration or in the following generations by using, forexample, co-transformation with two separate T-DNA constructs or sitespecific excision of the selection gene by a specific recombinase.

The present invention also relates to methods for producing abiologically active substance of the present invention comprising (a)cultivating a transgenic plant or a plant cell comprising a geneencoding a biologically active substance of the present invention underconditions conducive for production of the biologically activesubstance; and (b) recovering the biologically active substance.

Removal or Reduction of Biologically Active Substance

The present invention also relates to methods for producing a mutant ofa parent cell, which comprises disrupting or deleting all or a portionof a gene encoding a biologically active substance of the presentinvention, which results in the mutant cell producing less of thebiologically active substance than the parent cell when cultivated underthe same conditions.

The mutant cell may be constructed by reducing or eliminating expressionof a gene encoding or regulatory synthesis of a biologically activesubstance of the present invention using methods well known in the art,for example, insertions, disruptions, replacements, or deletions. Thegene to be modified or inactivated may be, for example, the codingregion or a part thereof essential for activity, or a regulatory elementof the gene required for the expression of the coding region. An exampleof such a regulatory or control sequence may be a promoter sequence or afunctional part thereof, i.e., a part that is sufficient for affectingexpression of the gene. Other control sequences for possiblemodification include, but are not limited to, a leader, propeptidesequence, signal peptide sequence, transcription terminator, andtranscriptional activator.

Modification or inactivation of the gene may be performed by subjectingthe parent cell to mutagenesis and selecting for mutant cells in whichexpression of the gene has been reduced or eliminated. The mutagenesis,which may be specific or random, may be performed, for example, by useof a suitable physical or chemical mutagenizing agent, by use of asuitable oligonucleotide, or by subjecting the DNA sequence to PCRgenerated mutagenesis. Furthermore, the mutagenesis may be performed byuse of any combination of these mutagenizing agents.

Examples of a physical or chemical mutagenizing agent suitable for thepresent purpose include ultraviolet (UV) irradiation, hydroxylamine,N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), O-methyl hydroxylamine,nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formicacid, and nucleotide analogues.

When such agents are used, the mutagenesis is typically performed byincubating the parent cell to be mutagenized in the presence of themutagenizing agent of choice under suitable conditions, and screeningand/or selecting for mutant cells exhibiting reduced or no expression ofthe gene.

Modification or inactivation of the nucleotide sequence may beaccomplished by introduction, substitution, or removal of one or morenucleotides in the gene or a regulatory element required for thetranscription or translation thereof. For example, nucleotides may beinserted or removed so as to result in the introduction of a stop codon,the removal of the start codon, or a change in the open reading frame.Such modification or inactivation may be accomplished by site-directedmutagenesis or PCR generated mutagenesis in accordance with methodsknown in the art. Although, in principle, the modification may beperformed in vivo, i.e., directly on the cell expressing the nucleotidesequence to be modified, it is preferred that the modification beperformed in vitro as exemplified below.

An example of a convenient way to eliminate or reduce expression of anucleotide sequence by a cell of choice is based on techniques of genereplacement, gene deletion, or gene disruption. For example, in the genedisruption method, a nucleic acid sequence corresponding to theendogenous nucleotide sequence is mutagenized in vitro to produce adefective nucleic acid sequence which is then transformed into theparent cell to produce a defective gene. By homologous recombination,the defective nucleic acid sequence replaces the endogenous nucleotidesequence. It may be desirable that the defective nucleotide sequencealso encodes a marker that may be used for selection of transformants inwhich the nucleotide sequence has been modified or destroyed. In aparticularly preferred aspect, the nucleotide sequence is disrupted witha selectable marker such as those described herein.

Alternatively, modification or inactivation of the nucleotide sequencemay be performed by established anti-sense techniques using a sequencecomplementary to the nucleotide sequence. More specifically, expressionof the nucleotide sequence by a cell may be reduced or eliminated byintroducing a sequence complementary to the nucleic acid sequence of thegene that may be transcribed in the cell and is capable of hybridizingto the mRNA produced in the cell. Under conditions allowing thecomplementary anti-sense nucleotide sequence to hybridize to the mRNA,the amount of protein translated is thus reduced or eliminated.

The present invention further relates to a mutant cell of a parent cellwhich comprises a disruption or deletion of a nucleotide sequenceencoding the biologically active substance or a control sequencethereof, which results in the mutant cell producing less of thebiologically active substance than the parent cell.

The biologically active substance-deficient mutant cells so created areparticularly useful as host cells for the expression of homologousand/or heterologous substances, such as polypeptides. Therefore, thepresent invention further relates to methods for producing a homologousor heterologous substance comprising (a) cultivating the mutant cellunder conditions conducive for production of the substance; and (b)recovering the substance. The term “heterologous substances” is definedherein as substances which are not native to the host cell, a nativesubstance in which modifications have been made to alter the nativesequence, or a native substance whose expression is quantitativelyaltered as a result of a manipulation of the host cell by recombinantDNA techniques.

In a further aspect, the present invention relates to a method forproducing a protein product essentially free of a biologically activesubstance by fermentation of a cell which produces both a biologicallyactive substance of the present invention as well as the protein productof interest by adding an effective amount of an agent capable ofinhibiting activity of the biologically active substance to thefermentation broth before, during, or after the fermentation has beencompleted, recovering the product of interest from the fermentationbroth, and optionally subjecting the recovered product to furtherpurification.

In accordance with this aspect of the invention, it is possible toremove at least 60%, preferably at least 75%, more preferably at least85%, still more preferably at least 95%, and most preferably at least99% of the biologically active substance. Complete removal ofbiologically active substance may be obtained by use of this method.

The methods used for cultivation and purification of the product ofinterest may be performed by methods known in the art.

The methods of the present invention for producing an essentiallybiologically active substance-free product is of particular interest inthe production of prokaryotic polypeptides, in particular bacterialproteins such as enzymes. The enzyme may be selected from, e.g., anamylolytic enzyme, lipolytic enzyme, proteolytic enzyme, cellulyticenzyme, oxidoreductase, or plant cell-wall degrading enzyme. Examples ofsuch enzymes include an aminopeptidase, amylase, amyloglucosidase,carbohydrase, carboxypeptidase, catalase, cellulase, chitinase,cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase,galactosidase, beta-galactosidase, glucoamylase, glucose oxidase,glucosidase, haloperoxidase, hemicellulase, invertase, isomerase,laccase, ligase, lipase, lyase, mannosidase, oxidase, pectinolyticenzyme, peroxidase, phytase, phenoloxidase, polyphenoloxidase,proteolytic enzyme, ribonuclease, transferase, transglutaminase, orxylanase. The biologically active substance-deficient cells may also beused to express heterologous proteins of pharmaceutical interest such ashormones, growth factors, receptors, and the like.

It will be understood that the term “prokaryotic polypeptides” includesnot only native polypeptides, but also those polypeptides, e.g.,enzymes, which have been modified by amino acid substitutions, deletionsor additions, or other such modifications to enhance activity,thermostability, pH tolerance and the like.

In a further aspect, the present invention relates to a product of aprotein or substance essentially free of a biologically active substanceof the invention, produced by a method of the present invention.

Compositions

The present invention also relates to compositions comprising abiologically active substance of the present invention. Preferably, thecompositions are enriched in the biologically active substance. The term“enriched” indicates that the biologically active substance of thecomposition has been increased, e.g., with an enrichment factor of 1.1.

The composition may comprise a biologically active substance of theinvention as the major component, e.g., a mono-component composition.Alternatively, the composition may comprise multiple biologically activesubstances, for example, multiple enzymes, such as an aminopeptidase,amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase,cutinase, cyclodextrin glycosyltransferase, deoxyribonuclease, esterase,alpha-galactosidase, beta-galactosidase, glucoamylase,alpha-glucosidase, beta-glucosidase, haloperoxidase, invertase, laccase,lipase, mannosidase, oxidase, pectinolytic enzyme, peptidoglutaminase,peroxidase, phytase, polyphenoloxidase, proteolytic enzyme,ribonuclease, transglutaminase, or xylanase.

The compositions may be prepared in accordance with methods known in theart and may be in the form of a liquid or a dry composition. Forinstance, the composition may be in the form of a granulate or amicrogranulate. The biologically active substance to be included in thecomposition may be stabilized in accordance with methods known in theart.

Methods for Using the Bacillus licheniformis Chromosome

The present invention also relates to methods for using the Bacilluslicheniformis chromosome.

The chromosome of Bacillus licheniformis serves as a reservoir of newgenes/proteins that have likely environmental, energy, health, andindustrial applications (e.g., enzymes, antibiotics, biochemicals). Aclear extension of this is that the newly discovered molecules can beused as starting points for further improvements via well-establishedgene shuffling, directed evolution, and protein engineering methods.Additionally, regions or motifs (e.g., signal peptides, active sites,substrate-binding regions) from the newly discovered molecules may beemployed to derive novel chimeras with industrially advantageousproperties.

The genes encoded in the chromosome may be used for monitoring globalgene expression during the life cycle of the organism or duringindustrial fermentations (e.g., implemented on DNA microarrays). Bymonitoring global gene expression, for example, improved processes forindustrial fermentation can be implemented with greater efficiency andeconomy.

The chromosome is useful in comparative evolutionary and ecologicalstudies. For example, dozens of Bacillus licheniformis isolates can bereadily compared on a global scale by hybridization of their genomicDNAs to a microarray fabricated from the reference strain presented inthis case (so-called comparative genomic hybridization). Using thismethod, one can compare various isolates to look forsimilarities/differences among geographical and environmental niches oramong biocontrol strains versus saprophytic isolates.

The chromosome sequence may be used to construct the metabolic blueprintfor Bacillus licheniformis that includes all catabolic and anabolicpathways, signaling pathways, regulatory networks, growth substrates,biochemical intermediates, end products, electron donors/acceptors andothers. In doing so, it is possible to modify the metabolic machinery ofthe organism by deleting unwanted pathways and/or addingenzymes/pathways from other organisms to generate useful chemicals andintermediates.

The pathways and components that contribute to production ofextracellular and surface proteins in Bacillus licheniformis can beextracted from the chromosomal sequence. This affords opportunities forimproved production of extracellular proteins by genetic manipulation ofthe secretion machinery.

The chromosome data allows deduction of the essential genes for Bacilluslicheniformis (either by comparison to related bacteria such as Bacillussubtilis or by systematic gene-by-gene knock outs). Thus it has becomepossible to design custom-made strains which contain only the genes thatare essential for production of specific proteins or metabolites(so-called cell factory concept).

The chromosome data may be used to construct interspecies hybridsbetween Bacillus licheniformis and other bacteria. Venter et al., 2003,Proc. Nat. Acad. Sci. USA 100, 15440-15445 have shown that it ispossible to construct an entire virus genome from smaller DNA segments.Thus, segments of the Bacillus licheniformis chromosome may be employedto derive novel chromosomal segments or even entire chimeric chromosomesfor specific applications.

In a preferred aspect, methods for using the Bacillus licheniformischromosome include host improvement, e.g., secretion of a protein ormetabolite, genome shuffling, construction of new genomes, metabolicengineering and pathway reconstruction, carrier for heterologousexpression vectors, microarrays as described herein, identification ofpolypeptides in proteomics analyses, and comparative genomics with otherBacillus species or related organisms.

Methods for Isolating Genes

The present invention also relates to methods for isolating a geneencoding a biologically active substance from a microbial strain. Themethod comprises first the addition of a mixture of first labelednucleic acid probes, isolated from a microbial strain cultured on mediumwithout an inducing substrate, and a mixture of second labeled nucleicacid probes, isolated from the microbial strain cultured on medium withthe inducing substrate, to an array of Bacillus licheniformis genesselected from the group consisting of nucleotides SEQ ID NOs: 2-4198,complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs:2-4198, under conditions where the labeled nucleic acid probes hybridizeto complementary sequences of the Bacillus licheniformis genes on thearray. The first nucleic acid probes are labeled with a first reporterand the second nucleic acid probes are labeled with a second reporter.The array is then examined under conditions wherein the relativeexpression of the genes of the microbial strain is determined by theobserved hybridization reporter signal of each spot on the array inwhich (i) the Bacillus licheniformis genes on the array that hybridizeto the first nucleic acid probes produce a distinct first hybridizationreporter signal or to the second nucleic acid probes produce a distinctsecond hybridization reporter signal, and (ii) the Bacilluslicheniformis genes on the array that hybridize to both the first andsecond nucleic acid probes produce a distinct combined hybridizationreporter signal. The probe is then sequenced to isolate from themicrobial strain the corresponding gene that encodes an enzyme thatdegrades or converts the substrate.

Enzymes. The gene of interest may encode any enzyme including anoxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase. Ina preferred aspect, the enzyme is an acylase, alpha-glucosidase,amidase, aminopeptidase, amylase, carbohydrase, carboxypeptidase,catalase, cellulase, chitinase, cutinase, cyclodextringlycosyltransferase, deoxyribonuclease, dextrinase, endoglucanase,esterase, galactanase, alpha-galactosidase, beta-galactosidase,glucoamylase, glucanase, glucocerebrosidase, alpha-glucosidase,beta-glucosidase, hemicellulase, invertase, laccase, lignase, lipase,lysin, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase,phosphatase, phospholipase, phytase, polyphenoloxidase, proteolyticenzyme, pullulanase, ribonuclease, transglutaminase, urokinase, orxylanase.

Inducing Substrate. The inducing substrate may be any substrate that issubject to the action of an enzyme, i.e., that degrades or converts thesubstrate. In a preferred aspect, the inducing substrate is lignin or alignin-containing material. In a more preferred aspect, thelignin-containing material is lignocellulose. In another preferredaspect, the inducing substrate is cellulose. In another preferredaspect, the inducing substrate is hemicellulose. In another preferredaspect, the inducing substrate is pectin. In another preferred aspect,the inducing substrate is a lipid. In another preferred aspect, theinducing substrate is phospholipid. In another preferred aspect, theinducing substrate is phytic acid. In another preferred aspect, theinducing substrate is protein. In another preferred aspect, the inducingsubstrate is a starch. In another preferred aspect, the inducingsubstrate is a medium that is low in nutrients such as amino acids,carbon, nitrogen, phosphate, or iron.

In a more preferred aspect, the protein substrate is blood, casein, egg,gelatin, gluten, milk protein, or soy protein. In another more preferredaspect, the lignin-containing material is hardwood thermomechanicalpulp. In another more preferred aspect, the lignocellulose is cornstover. In another more preferred aspect, the lignocellulose is whitepoplar. In another more preferred aspect, the lignocellulose is ricestraw. In another more preferred aspect, the lignocellulose is switchgrass.

Microbial Strains. In the methods of the present invention, themicrobial strain may be any microbial strain. The strain is cultured ona suitable nutrient medium with and without a substrate of interest. Thestrain cultured on medium without the substrate is used as a referencefor identifying differences in expression of the same or similarcomplement of genes in the strain cultured on medium with substrate. Thestrain may be a wild-type, mutant, or recombinant strain.

In the methods of the present invention, the microbial strain ispreferably a bacterium. In a more preferred aspect, the bacterium is aBacillus, Pseudomonas, or Streptomyces strain or E. coli.

The Bacillus strain may be any Bacillus strain. In a preferred aspect,the Bacillus strain is Bacillus alkalophilus, Bacillusamyloliquefaciens, Bacillus brevis, Bacillus cereus, Bacillus circulans,Bacillus clausii, Bacillus coagulans, Bacillus fastidiosus, Bacillusfirmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis,Bacillus macerans, Bacillus megaterium, Bacillus methanolicus, Bacilluspumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillussubtilis, or Bacillus thuringiensis. It will be understood that the term“Bacillus” also encompasses relatives of Bacillus such as Paenibacillus,Oceanobacillus, and the like.

The Pseudomonas strain may be any Pseudomonas strain. In a preferredaspect, the Pseudomonas strain is Pseudomonas acidovorans, Pseudomonasaeruginosa, Pseudomonas alcaligenes, Pseudomonas anguilliseptica,Pseudomonas abtimicrobica, Pseudomonas aurantiaca, Pseudomonasaureofaciens, Pseudomonas beijerinckii, Pseudomonas boreopolis,Pseudomonas chlororaphis, Pseudomonas citronellolis, Pseudomonascocovenenans, Pseudomonas diminuta, Pseudomonas doudoroffii, Pseudomonasechinoides, Pseudomonas elongata, Pseudomonas fluorescens, Pseudomonasfragi, Pseudomonas halophobica, Pseudomonas huttiensis, Pseudomonasindigofera, Pseudomonas lanceolata, Pseudomonas lemoignei, Pseudomonaslundensis, Pseudomonas mendocina, Pseudomonas mephitica, Pseudomonasmucidolens, Pseudomonas oleovorans, Pseudomonas phenazinium, Pseudomonaspictorium, Pseudomonas putida, Pseudomonas resinovorans, Pseudomonassaccharophila, Pseudomonas stanieri, Pseudomonas stutzeri, Pseudomonastaetrolens, or Pseudomonas vesicularis.

The Streptomyces strain may be any Streptomyces strain. In a preferredaspect, the Streptomyces strain is Streptomyces lividans. In anotherpreferred aspect, the Streptomyces strain is Streptomyces murinus.

Microarrays. The term “an array of Bacillus licheniformis genes” isdefined herein as a linear or two-dimensional array of preferablydiscrete elements of an array of Bacillus licheniformis genes selectedfrom the group consisting of nucleotides SEQ ID NOs: 2-4198,complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQ ID NOs:2-4198 (e.g., synthetic oligonucleotides of, for example, 40-60nucleotides), wherein each discrete element has a finite area, formed onthe surface of a solid support. It shall be understood that the term“Bacillus licheniformis genes” encompasses nucleotides SEQ ID NOs:2-4198, complementary strands of SEQ ID NOs: 2-4198, or fragments of SEQID NOs: 2-4198. The term “microarray” is defined herein as an array ofBacillus licheniformis gene elements having a density of discrete ofBacillus licheniformis gene elements of at least about 100/cm², andpreferably at least about 1000/cm². The Bacillus licheniformis geneelements in a microarray have typical dimensions, e.g., diameters, inthe range of between about 10 to about 250 μm, preferably in the rangeof between about 10 to about 200 μm, more preferably in the range ofbetween about 20 to about 150 μm, even more preferably in the range ofbetween about 20 to about 100 μm, most preferably in the range ofbetween about 50 to about 100 μm, and even most preferably in the rangeof between about 80 to about 100 μm, and are separated from other geneelements in the microarray by about the same distance.

Methods and instruments for forming microarrays on the surface of asolid support are well known in the art. See, for example, U.S. Pat. No.5,807,522; U.S. Pat. No. 5,700,637; and U.S. Pat. No. 5,770,151. Theinstrument may be an automated device such as described in U.S. Pat. No.5,807,522.

The term “a substrate containing an array of Bacillus licheniformisgenes” is defined herein as a solid support having deposited on thesurface of the support one or more of a plurality of Bacilluslicheniformis genes, as described herein, for use in detecting bindingof labeled nucleic acids to the Bacillus licheniformis genes.

The substrate may, in one aspect, be a glass support (e.g., glass slide)having a hydrophilic or hydrophobic coating on the surface of thesupport, and an array of distinct random nucleic acid fragments bound tothe coating, where each distinct random nucleic acid fragment isdisposed at a separate, defined position.

Each microarray in the substrate preferably contains at least 10³distinct Bacillus licheniformis in a surface area of less than about 5or 6 cm². Each distinct Bacillus licheniformis gene (i) is disposed at aseparate, defined position on the array, (ii) has a length of at least50 bp, and (iii) is present in a defined amount between about 0.1femtomoles and 100 nanomoles or higher if necessary.

For a hydrophilic coating, the glass slide is coated by placing a filmof a polycationic polymer with a uniform thickness on the surface of theslide and drying the film to form a dried coating. The amount ofpolycationic polymer added should be sufficient to form at least amonolayer of polymers on the glass surface. The polymer film is bound tothe surface via electrostatic binding between negative silyl-OH groupson the surface and charged cationic groups in the polymers. Suchpolycationic polymers include, but are not limited to, polylysine andpolyarginine.

Another coating strategy employs reactive aldehydes to couple DNA to theslides (Schena et al., 1996, Proceedings of the National Academy ofScience USA 93: 10614-10619; Heller at al., 1997, Proceedings of theNational Academy of Science USA 94: 2150-2155).

Alternatively, the surface may have a relatively hydrophobic character,i.e., one that causes aqueous medium deposited on the surface to bead. Avariety of known hydrophobic polymers, such as polystyrene,polypropylene, or polyethylene, have desirable hydrophobic properties,as do glass and a variety of lubricant or other hydrophobic films thatmay be applied to the support surface. A support surface is“hydrophobic” if an aqueous droplet applied to the surface does notspread out substantially beyond the area size of the applied droplet,wherein the surface acts to prevent spreading of the droplet applied tothe surface by hydrophobic interaction with the droplet.

In another aspect, the substrate may be a multi-cell substrate whereeach cell contains a microarray of Bacillus licheniformis and preferablyan identical microarray, formed on a porous surface. For example, a96-cell array may typically have array dimensions between about 12 and244 mm in width and 8 and 400 mm in length, with the cells in the arrayhaving width and length dimension of 1/12 and ⅛ the array width andlength dimensions, respectively, i.e., between about 1 and 20 in widthand 1 and 50 mm in length.

The solid support may include a water-impermeable backing such as aglass slide or rigid polymer sheet, or other non-porous material. Formedon the surface of the backing is a water-permeable film, which is formedof porous material. Such porous materials include, but are not limitedto, nitrocellulose membrane nylon, polypropylene, and polyvinylidenedifluoride (PVDF) polymer. The thickness of the film is preferablybetween about 10 and 1000 μm. The film may be applied to the backing byspraying or coating, or by applying a preformed membrane to the backing.

Alternatively, the solid support may be simply a filter composed ofnitrocellulose, nylon, polypropylene, or polyvinylidene difluoride(PVDF) polymer, or, for that matter, any material suitable for use.

The film surface may be partitioned into a desirable array of cells bywater-impermeable grid lines typically at a distance of about 100 to2000 μm above the film surface. The grid lines can be formed on thesurface of the film by laying down an uncured flowable resin orelastomer solution in an array grid, allowing the material to infiltratethe porous film down to the backing, and then curing the grid lines toform the cell-array substrate.

The barrier material of the grid lines may be a flowable silicone,wax-based material, thermoset material (e.g., epoxy), or any otheruseful material. The grid lines may be applied to the solid supportusing a narrow syringe, printing techniques, heat-seal stamping, or anyother useful method known in the art.

Each well preferably contains a microarray of distinct Bacilluslicheniformis genes. “Distinct Bacillus licheniformis genes” as appliedto the genes forming a microarray is defined herein as an array memberwhich is distinct from other array members on the basis of a differentBacillus licheniformis gene sequence or oligo sequence thereof, and/ordifferent concentrations of the same or distinct Bacillus licheniformisgenes and/or different mixtures of distinct Bacillus licheniformis genesor different-concentrations of Bacillus licheniformis genes. Thus anarray of “distinct Bacillus licheniformis genes” may be an arraycontaining, as its members, (i) distinct Bacillus licheniformis geneswhich may have a defined amount in each member, (ii) different, gradedconcentrations of a specific Bacillus licheniformis gene, and/or (iii)different-composition mixtures of two or more distinct Bacilluslicheniformis genes.

It will be understood, however, that in the methods of the presentinvention, any type of substrate known in the art may be used.

The delivery of a known amount of a selected Bacillus licheniformis geneto a specific position on the support surface is preferably performedwith a dispensing device equipped with one or more tips for insuringreproducible deposition and location of the Bacillus licheniformis genesand for preparing multiple arrays. Any dispensing device known in theart may be used in the methods of the present invention. See, forexample, U.S. Pat. No. 5,807,522.

For liquid-dispensing on a hydrophilic surface, the liquid will haveless of a tendency to bead, and the dispensed volume will be moresensitive to the total dwell time of the dispenser tip in the immediatevicinity of the support surface.

For liquid-dispensing on a hydrophobic surface, flow of fluid from thetip onto the support surface will continue from the dispenser onto thesupport surface until it forms a liquid bead. At a given bead size,i.e., volume, the tendency of liquid to flow onto the surface will bebalanced by the hydrophobic surface interaction of the bead with thesupport surface, which acts to limit the total bead area on the surface,and by the surface tension of the droplet, which tends toward a givenbead curvature. At this point, a given bead volume will have formed, andcontinued contact of the dispenser tip with the bead, as the dispensertip is being withdrawn, will have little or no effect on bead volume.

The desired deposition volume, i.e., bead volume, formed is preferablyin the range 2 pl (picoliters) to 2 nl (nanoliters), although volumes ashigh as 100 nl or more may be dispensed. It will be appreciated that theselected dispensed volume will depend on (i) the “footprint” of thedispenser tip(s), i.e., the size of the area spanned by the tip(s), (ii)the hydrophobicity of the support surface, and (iii) the time of contactwith and rate of withdrawal of the tip(s) from the support surface. Inaddition, bead size may be reduced by increasing the viscosity of themedium, effectively reducing the flow time of liquid from the dispensingdevice onto the support surface. The drop size may be furtherconstrained by depositing the drop in a hydrophilic region surrounded bya hydrophobic grid pattern on the support surface.

At a given tip size, bead volume can be reduced in a controlled fashionby increasing surface hydrophobicity, reducing time of contact of thetip with the surface, increasing rate of movement of the tip away fromthe surface, and/or increasing the viscosity of the medium. Once theseparameters are fixed, a selected deposition volume in the desiredpicoliter to nanoliter range can be achieved in a repeatable fashion.

After depositing a liquid droplet of a Bacillus licheniformis genesample at one selected location on a support, the tip may be moved to acorresponding position on a second support, the Bacillus licheniformisgene sample is deposited at that position, and this process is repeateduntil the random nucleic acid fragment sample has been deposited at aselected position on a plurality of supports.

This deposition process may then be repeated with another random nucleicacid fragment sample at another microarray position on each of thesupports.

The diameter of each Bacillus licheniformis gene region is preferablybetween about 20-200 μm. The spacing between each region and its closest(non-diagonal) neighbor, measured from center-to-center, is preferablyin the range of about 20-400 μm. Thus, for example, an array having acenter-to-center spacing of about 250 μm contains about 40 regions/cm or1,600 regions/cm². After formation of the array, the support is treatedto evaporate the liquid of the droplet forming each region, to leave adesired array of dried, relatively flat Bacillus licheniformis gene oroligo thereof regions. This drying may be done by heating or undervacuum. The DNA can also be UV-crosslinked to the polymer coating.

Nucleic Acid Probes. In the methods of the present invention, thestrains are cultivated in a nutrient medium with and without a substrateusing methods well known in the art for isolation of nucleic acids to beused as probes. For example, the strains may be cultivated by shakeflask cultivation, small-scale or large-scale fermentation (includingcontinuous, batch, fed-batch, or solid state fermentations) inlaboratory or industrial fermentors performed in a suitable medium. Thecultivation takes place in a suitable nutrient medium comprising carbonand nitrogen sources and inorganic salts, using procedures known in theart. Suitable media are available from commercial suppliers or may beprepared according to published compositions (e.g., in catalogues of theAmerican Type Culture Collection).

The nucleic acid probes from the microbial strains cultured on mediumwith and without substrate may be any nucleic acid including genomicDNA, cDNA, and RNA, and may be isolated using standard methods known inthe art.

The populations of isolated nucleic acid probes may be labeled withdetection reporters such as colorimetric, radioactive for example, ³²P,³³P, or ³⁵S), fluorescent reporters, or other reporters using methodsknown in the art (Chen et al., 1998, Genomics 51: 313-324; DeRisi etal., 1997, Science 278: 680-686; U.S. Pat. No. 5,770,367).

In a preferred aspect, the probes are labeled with fluorescentreporters. For example, the DNA probes may be labeled during reversetranscription from the respective RNA pools by incorporation offluorophores as dye-labeled nucleotides (DeRisi et al., 1997, supra),e.g., Cy5-labeled deoxyuridine triphosphate, or the isolated cDNAs maybe directly labeled with different fluorescent functional groups.Fluorescent-labeled nucleotides include, but are not limited to,fluorescein conjugated nucleotide analogs (green fluorescence),lissamine nucleotide analogs (red fluorescence). Fluorescent functionalgroups include, but are not limited to, Cy3 (a green fluorescent dye)and Cy5 (red fluorescent dye).

Array Hybridization. The labeled nucleic acids from the two strainscultivated with and without substrate are then added to an array ofBacillus licheniformis genes under conditions where the nucleic acidpools from the two strains hybridize to complementary sequences of theBacillus licheniformis genes on the array. For purposes of the presentinvention, hybridization indicates that the labeled nucleic acids fromthe two strains hybridize to the Bacillus licheniformis genes under verylow to very high stringency conditions.

A small volume of the labeled nucleic acids mixture is loaded onto thesubstrate. The solution will spread to cover the entire microarray. Inthe case of a multi-cell substrate, one or more solutions are loadedinto each cell which stop at the barrier elements.

For nucleic acid probes of at least about 100 nucleotides in length,miroarray hybridization conditions described by Eisen and Brown, 1999,Methods of Enzymology 303: 179-205, may be used. Hybridization isconducted under a cover slip at 65° C. in 3×SSC for 4-16 hours followedby post-hybridization at room temperature after removal of the coverslip in 2×SSC, 0.1% SDS by washing the array two or three times in thesolution, followed by successive washes in 1×SSC for 2 minutes and0.2×SSC wash for two or more minutes.

Conventional conditions of very low to very high stringency conditionsmay also be used. Very low to very high stringency conditions aredefined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3%SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25%formamide for very low and low stringencies, 35% formamide for mediumand medium-high stringencies, or 50% formamide for high and very highstringencies, following standard Southern blotting procedures.

The carrier material is finally washed three times each for 15 minutesusing 2×SSC, 0.2% SDS preferably at least at 45° C. (very lowstringency), more preferably at least at 50° C. (low stringency), morepreferably at least at 55° C. (medium stringency), more preferably atleast at 60° C. (medium-high stringency), even more preferably at leastat 65° C. (high stringency), and most preferably at least at 70° C.(very high stringency).

For shorter nucleic acid probes which are less than 50 nucleotides,microarray hybridization conditions described by Kane et al., 2000,Nucleic Acids Research 28: 4552-4557, may be used. Hybridization isconducted under a supported coverslip at 42° C. for 16-18 hours at highhumidity in 50% formamide, 4.1×Denhardt's solution, 4.4×SSC, and 100μg/ml of herring sperm DNA. Arrays are washed after removal of thecoverslip in 4×SSC by immersion into 1×SSC, 0.1% SDS for 10 minutes,0.1×SSC, 0.1% SDS twice for 10 minutes, and 0.1×SSC twice for 10minutes.

For shorter nucleic acid probes which are about 50 nucleotides to about100 nucleotides in length, conventional stringency conditions may beused. Such stringency conditions are defined as prehybridization,hybridization, and washing post-hybridization at 5° C. to 10° C. belowthe calculated T_(m) using the calculation according to Bolton andMcCarthy (1962, Proceedings of the National Academy of Sciences USA48:1390) in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40,1×Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasicphosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml following standardSouthern blotting procedures.

The carrier material is finally washed once in 6×SSC plus 0.1% SDS for15 minutes and twice each for 15 minutes using 6×SSC at 5° C. to 10° C.below the calculated T_(m).

The choice of hybridization conditions will depend on the degree ofhomology between the Bacillus licheniformis genes and the nucleic acidprobes obtained from the strain cultured with and without inducingsubstrate. For example, where the nucleic acid probes and the Bacilluslicheniformis genes are obtained from identical strains, high stringencyconditions may be most suitable. Where the strains are from a genus orspecies different from which the Bacillus licheniformis genes wereobtained, low or medium stringency conditions may be more suitable.

In a preferred aspect, the hybridization is conducted under lowstringency conditions. In a more preferred aspect, the hybridization isconducted under medium stringency conditions. In a most preferredaspect, the hybridization is conducted under high stringency conditions.

The entire solid support is then reacted with detection reagents ifneeded and analyzed using standard colorimetric, radioactive, orfluorescent detection means. All processing and detection steps areperformed simultaneously to all of the microarrays on the solid supportensuring uniform assay conditions for all of the microarrays on thesolid support.

Detection. The most common detection method is laser-inducedfluorescence detection using confocal optics (Cheung et al., 1998, Nat.Genet. 18: 225-230). The array is examined under fluorescence excitationconditions such that (i) the Bacillus licheniformis genes on the arraythat hybridize to the first nucleic acid probes obtained from the straincultured without inducing substrate and to the second nucleic acidprobes obtained from the strain cultured with inducing substrate producea distinct first fluorescence emission color and a distinct secondfluorescence emission color, respectively, and (ii) the Bacilluslicheniformis genes on the array that hybridize to substantially equalnumbers of nucleic acid probes obtained from the strain cultured withoutinducing substrate and from the strain cultured with inducing substrateproduce a distinct combined fluorescence emission color; wherein therelative expression of the genes in the strains can be determined by theobserved fluorescence emission color of each spot on the array.

The fluorescence excitation conditions are based on the selection of thefluorescence reporters. For example, Cy3 and Cy5 reporters are detectedwith solid state lasers operating at 532 nm and 632 nm, respectively.

However, other methods of detection well known in the art may be usedsuch as standard photometric, colorimetric, or radioactive detectionmeans, as described earlier.

Data Analysis. The data obtained from the scanned image may then beanalyzed using any of the commercially available image analysissoftware. The software preferably identifies array elements, subtractsbackgrounds, deconvolutes multi-color images, flags or removesartifacts, verifies that controls have performed properly, andnormalizes the signals (Chen et al., 1997, Journal of Biomedical Optics2: 364-374).

Several computational methods have been described for the analysis andinterpretation of microarray-based expression profiles including clusteranalysis (Eisen et al., 1998, Proc. Nat. Acad. Sci. USA 95:14863-14868), parametric ordering of genes (Spellman et al., 1998, Mol.Biol. Cell 9: 3273-3297), and supervised clustering methods based onrepresentative hand-picked or computer-generated expression profiles(Chu et al., 1998. Science 282: 699-705). Preferred methods forevaluating the results of the microarrays employ statistical analysis todetermine the significance of the differences in expression levels. Inthe methods of the present invention, the difference in the detectedexpression level is at least about 10% or greater, preferably at leastabout 20% or greater, more preferably at least about 50% or greater,even more preferably at least about 75% or greater; and most preferablyat least about 100% or greater.

One such preferred system is the Significance Analysis of Microarrays(SAM) (Tusher et al., 2001, Proc. Natl. Acad. Sci. USA 98: 5116-5121).Statistical analysis allows the determination of significantly alteredexpression of levels of about 50% or even less. The PAM (or predictiveanalysis for microarrays) represents another approach for analyzing theresults of the microarrays (Tibshirani et al., 2002, Proc. Natl. Acad.Sci. USA 99: 6567-6572).

Cluster algorithms may also be used to analyze microarray expressiondata. From the analysis of the expression profiles it is possible toidentify co-regulated genes that perform common metabolic orbiosynthetic functions. Hierarchical clustering has been employed in theanalysis of microarray expression data in order to place genes intoclusters based on sharing similar patterns of expression (Eisen et al.,1998, supra). This method yields a graphical display that resembles akind of phylogenetic tree where the relatedness of the expressionbehavior of each gene to every other gene is depicted by branch lengths.The programs Cluster and TreeView, both written by Michael Eisen (Eisenet al., 1998 Proc. Nat. Acad. Sci. USA 95: 14863-14868) are freelyavailable. Genespring is a commercial program available for suchanalysis (Silicon Genetics, Redwood City, Calif.),

Self-organizing maps (SOMs), a non-hierarchical method, have also beenused to analyze microarray expression data (Tamayo et al., 1999, Proc.Natl. Acad. Sci. USA 96: 2907-2912). This method involves selecting ageometry of nodes, where the number of nodes defines the number ofclusters. Then, the number of genes analyzed and the number ofexperimental conditions that were used to provide the expression valuesof these genes are subjected to an iterative process (20,000-50,000iterations) that maps the nodes and data points into multidimensionalgene expression space. After the identification of significantlyregulated genes, the expression level of each gene is normalized acrossexperiments. As a result, the expression profile of the genome ishighlighted in a manner that is relatively independent of each gene'sexpression magnitude. Software for the “GENECLUSTER” SOM program formicroarray expression analysis can be obtained from the Whitehead/MITCenter for Genome Research. SOMs can also be constructed using theGeneSpring software package.

Isolation of Genes. Probes containing genes or portions thereofidentified to be induced by the present of substrate in the medium arecharacterized by determining the sequence of the probe. Based on thesequence, the gene can then be isolated using methods well known in theart.

The techniques used to isolate or clone a gene include isolation fromgenomic DNA, preparation from cDNA, or a combination thereof. Thecloning of the gene from such genomic DNA can be effected, e.g., byusing the well known polymerase chain reaction (PCR) or antibodyscreening of expression libraries to detect cloned DNA fragments withshared structural features. See, e.g., Innis et al., 1990, PCR: A Guideto Methods and Application, Academic Press, New York. Other nucleic acidamplification procedures such as ligase chain reaction (LCR), ligatedactivated transcription (LAT) and nucleic acid sequence-basedamplification (NASBA) may be used. The gene may be cloned from thestrain of interest, or another or related organism and thus, forexample, may be an allelic or species variant of the gene.

Methods for Monitoring Differential Expression of a Plurality of Genes

The present invention also relates to methods for monitoringdifferential expression of a plurality of genes in a first bacterialcell relative to expression of the same genes in one or more secondbacterial cells, comprising:

(a) adding a mixture of detection reporter-labeled nucleic acidsisolated from the bacterial cells to a substrate containing an array ofBacillus licheniformis genes selected from the group consisting ofnucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs:2-4198, or fragments of SEQ ID NOs: 2-4198, under conditions where thedetection reporter-labeled nucleic acids hybridize to complementarysequences of the Bacillus licheniformis genes on the array, wherein thenucleic acids from the first bacterial cell and the one or more secondbacterial cells are labeled with a first detection reporter and one ormore different second detection reporters, respectively; and

(b) examining the array under conditions wherein the relative expressionof the genes in the bacterial cells is determined by the observeddetection signal of each spot on the array in which (i) the Bacilluslicheniformis genes on the array that hybridize to the nucleic acidsobtained from either the first or the one or more second bacterial cellsproduce a distinct first detection signal or one or more seconddetection signals, respectively, and (ii) the Bacillus licheniformisgenes on the array that hybridize to the nucleic acids obtained fromboth the first and one or more second bacterial produce a distinctcombined detection signal.

The methods of the present invention may be used to monitor globalexpression of a plurality of genes from a Bacillus cell, discover newgenes, identify possible functions of unknown open reading frames, andmonitor gene copy number variation and stability. For example, theglobal view of changes in expression of genes may be used to provide apicture of the way in which Bacillus cells adapt to changes in cultureconditions, environmental stress, or other physiological provocation.Other possibilities for monitoring global expression include sporemorphogenesis, recombination, metabolic or catabolic pathwayengineering.

The methods of the present invention are particularly advantageous sinceone spot on an array equals one gene or open reading frame becauseextensive follow-up characterization is unnecessary since sequenceinformation is available, and the Bacillus licheniformis microarrays canbe organized based on function of the gene products.

Microarrays. Methods for preparing the microarrays are described herein.

Bacterial Cells. In the methods of the present invention, the two ormore Bacillus cells may be any Bacillus cell where one of the cells isused as a reference for identifying differences in expression of thesame or similar complement of genes in the other cell(s). In one aspect,the two or more cells are the same cell. For example, they may becompared under different growth conditions, e.g., oxygen limitation,nutrition, and/or physiology. In another aspect, one or more cells aremutants of the reference cell. For example, the mutant(s) may have adifferent phenotype. In a further aspect, the two or more cells are ofdifferent species (e.g., Bacillus clausii and Bacillus subtilis). Inanother further aspect, the two or more cells are of different genera.In an even further aspect, one or more cells are transformants of thereference cell, wherein the one or more transformants exhibit adifferent property. For example, the transformants may have an improvedphenotype relative to the reference cell and/or one of the othertransformants. The term “phenotype” is defined herein as an observableor outward characteristic of a cell determined by its genotype andmodulated by its environment. Such improved phenotypes may include, butare not limited to, improved secretion or production of a protein orcompound, reduced or no secretion or production of a protein orcompound, improved or reduced expression of a gene, desirablemorphology, an altered growth rate under desired conditions, relief ofover-expression mediated growth inhibition, or tolerance to low oxygenconditions.

The Bacillus cells may be any Bacillus cells, but preferably Bacillusalkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacilluscereus, Bacillus circulans, Bacillus clausii, Bacillus coagulans,Bacillus fastidiosus, Bacillus firmus, Bacillus lautus, Bacillus lentus,Bacillus licheniformis, Bacillus macerans, Bacillus megaterium, Bacillusmethanolicus, Bacillus pumilus, Bacillus sphaericus, Bacillusstearothermophilus, Bacillus subtilis, or Bacillus thuringiensis cells.

In a preferred aspect, the Bacillus cells are Bacillus alkalophiluscells. In another preferred aspect, the Bacillus cells are Bacillusamyloliquefaciens cells. In another preferred aspect, the Bacillus cellsare Bacillus brevis cells. In another preferred aspect, the Bacilluscells are Bacillus cereus cells In another preferred aspect, theBacillus cells are Bacillus circulans cells. In another preferredaspect, the Bacillus cells are Bacillus clausii cells. In anotherpreferred aspect, the Bacillus cells are Bacillus coagulans cells. Inanother preferred aspect, the Bacillus cells are Bacillus fastidiosuscells. In another preferred aspect, the Bacillus cells are Bacillusfirmus cells. In another preferred aspect, the Bacillus cells areBacillus lautus cells. In another preferred aspect, the Bacillus cellsare Bacillus lentus cells. In another preferred aspect, the Bacilluscells are Bacillus licheniformis cells. In another preferred aspect, theBacillus cells are Bacillus macerans cells. In another preferred aspect,the Bacillus cells are Bacillus megaterium cells. In another preferredaspect, the Bacillus cells are Bacillus methanolicus cells. In anotherpreferred aspect, the Bacillus cells are Bacillus pumilus cells. Inanother preferred aspect, the Bacillus cells are Bacillus sphaericuscells. In another preferred aspect, the Bacillus cells are Bacillusstearothermophilus cells. In another preferred aspect, the Bacilluscells are Bacillus subtilis cells. In another preferred aspect, theBacillus cells are Bacillus thuringiensis cells.

In a more preferred aspect, the Bacillus cells are Bacilluslicheniformis cells. In a most preferred aspect, the Bacilluslicheniformis cells are Bacillus licheniformis ATCC 14580 cells.

In another more preferred aspect, the Bacillus cells are Bacillusclausii cells. In another most preferred aspect, the Bacillus clausiicells are Bacillus clausii NUB 10309 cells.

It will be understood that the term “Bacillus” also encompassesrelatives of Bacillus such as Paenibacillus, Oceanobacillus, and thelike.

In the methods of the present invention, the cells are cultivated in anutrient medium suitable for growth using methods well known in the artfor isolation of the nucleic acids to be used as probes. For example,the cells may be cultivated by shake flask cultivation, small-scale orlarge-scale fermentation (including continuous, batch, fed-batch, orsolid state fermentations) in laboratory or industrial fermentorsperformed in a suitable medium. The cultivation takes place in asuitable nutrient medium comprising carbon and nitrogen sources andinorganic salts, using procedures known in the art. Suitable media areavailable from commercial suppliers or may be prepared according topublished compositions (e.g., in catalogues of the American Type CultureCollection).

Nucleic Add Probes. The nucleic acid probes from the two or moreBacillus cells may be any nucleic acid including genomic DNA, cDNA, andRNA, and may be isolated using standard methods known in the art, asdescribed herein. The populations of isolated nucleic acid probes may belabeled with colorimetric, radioactive, fluorescent reporters, or otherreporters using methods described herein.

In a preferred aspect, the probes are labeled with fluorescentreporters, e.g., Cy3 (a green fluorescent dye) and Cy5 (red fluorescentdye), as described herein.

Array Hybridization. The labeled nucleic acids from the two or moreBacillus cells are then added to a substrate containing an array ofBacillus licheniformis genes under conditions, as described herein,where the nucleic acid pools from the two or more Bacillus cellshybridize to complementary sequences of the Bacillus licheniformis geneson the array.

Detection and Data Analysis. The same methods as described herein areused for detection and data analysis.

Computer Readable Media and Computer-Based Systems

The Bacillus licheniformis chromosome and its genes described herein maybe “provided” in a variety of media to facilitate their use. The term“provided” refers to a manufacture comprising an array of Bacilluslicheniformis genes. Such manufactures provide the Bacilluslicheniformis genes in a form which allows one skilled in the art toexamine the manufacture using means not directly applicable to examiningthe chromosome or a subset thereof as it exists in nature or in purifiedform.

Thus, the present invention also relates to such a manufacture in theform of a computer readable medium comprising an array of Bacilluslicheniformis genes selected from the group consisting of nucleotidesSEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs: 2-4198, orfragments of SEQ ID NOs: 2-4198.

In one application of this aspect, the Bacillus licheniformis genes ofthe present invention can be recorded on computer readable media. Theterm “computer readable media” is defined herein as any medium which canbe read and accessed by a computer. Such computer readable mediainclude, but are not limited to, magnetic storage media, e.g., floppydiscs, hard disc storage medium, and magnetic tape; optical storagemedia, e.g., CD-ROM, DVD; electrical storage media, e.g., RAM and ROM;and hybrids of these categories, e.g., magnetic/optical storage media.One skilled in the art can readily appreciate how any of the presentlyknown computer readable media can be used to create a manufacturecomprising computer readable medium having recorded thereon a nucleotidesequence of the present invention. Likewise, it will be clear to thoseof skill how additional computer readable media that may be developedalso can be used to create analogous manufactures having recordedthereon a nucleotide sequence of the present invention.

As used herein, “recorded” refers to a process for storing informationon computer readable medium. One skilled in the art can readily adoptany of the presently known methods for recording information on computerreadable medium to generate manufactures comprising the nucleotidesequence information of the present invention.

A variety of data storage structures are available for creating acomputer readable medium having recorded thereon a nucleotide sequenceof the present invention. The choice of the data storage structure willgenerally be based on the means chosen to access the stored information.In addition, a variety of data processor programs and formats can beused to store the nucleotide sequence information of the presentinvention on computer readable medium. The sequence information can berepresented in a word processing text file, formatted incommercially-available software such as WordPerfect and Microsoft Word,or represented in the form of an ASCII file, stored in a databaseapplication, such as DB2, Sybase, Oracle, or the like. A skilled artisancan readily adapt any number of data-processor structuring formats(e.g., text file or database) in order to obtain computer readablemedium having recorded thereon the nucleotide sequence information ofthe present invention.

Various computer software programs are publicly available that allow askilled artisan to access sequence information provided in a computerreadable medium. Thus, by providing in computer readable form an arrayof Bacillus licheniformis genes selected from the group consisting ofnucleotides SEQ ID NOs: 2-4198, complementary strands of SEQ ID NOs:2-4198, or fragments of SEQ ID NOs: 2-4198, enables one skilled in theart to routinely access the provided sequence information for a widevariety of purposes.

Software utilizing the BLAST (Altschul et al., 1990, supra), BLAZE(Brutlag et al., 1993, Comp. Chem. 17: 203-207), GENEMARK (Lukashin andBorodovsky, 1998, Nucleic Acids Research 26: 1107-1115), GENSCAN (Burgeand Karlin, 1997, Journal of Molecular Biology 268: 78-94), GLIMMER(Salzberg et al., 1998, Nucleic Acids Research 26: 544-548), and GRAIL(Xu et al., 1994, Comput. Appl. Biosci. 10: 613-623) search algorithmsmay be used to identify open reading frames (ORFs) within a genome ofinterest, which contain homology to ORFs or proteins from both Bacilluslicheniformis and Bacillus clausii and from other organisms. Among theORFs discussed herein are protein encoding fragments of the Bacilluslicheniformis and Bacillus clausii genomes useful in producingcommercially important proteins, such as enzymes used in fermentationreactions and in the production of commercially useful metabolites.

The present invention further provides systems, particularlycomputer-based systems, which contain the sequence information describedherein. Such systems are designed to identify, among other things, genesand gene products—many of which could be products themselves or used togenetically modify an industrial expression host through increased ordecreased expression of a specific gene sequence(s).

The term “a computer-based system” is herein defined as the hardwaremeans, software means, and data storage means used to analyze thenucleotide sequence information of the present invention. The minimumhardware means of the computer-based systems of the present inventioncomprises a central processing unit (CPU), input means, output means,and data storage means. One skilled in the art can readily appreciatethat any currently available computer-based system is suitable for usein the present invention.

As stated above, the computer-based systems of the present inventioncomprise a data storage means having stored therein a nucleotidesequence of the present invention and the necessary hardware means andsoftware means for supporting and implementing a search means.

The term “data storage means” is defined herein as memory which canstore nucleotide sequence information of the present invention, or amemory access means which can access manufactures having recordedthereon the nucleotide sequence information of the present invention.

The term “search means” refers is defined herein as one or more programswhich are implemented on the computer-based system to compare a targetsequence or target structural motif with the sequence information storedwithin the data storage means. Search means are used to identifyfragments or regions of the present genomic sequences which match aparticular target sequence or target motif. A variety of knownalgorithms are disclosed publicly and a variety of commerciallyavailable software for conducting search means are and can be used inthe computer-based systems of the present invention. Examples of suchsoftware includes, but is not limited to, MacPattern (Fuchs, 1991,Comput. Appl. Biosci. 7: 105-106), BLASTN and BLASTX National Center forBiotechnology information (NCBI). One skilled in the art can readilyrecognize that any one of the available algorithms or implementingsoftware packages for conducting homology searches can be adapted foruse in the present computer-based systems.

The term “target sequence” is defined here as any DNA (genomic DNA,cDNA) or amino acid sequence of six or more nucleotides or two or moreamino acids. One skilled in the art can readily recognize that thelonger a target sequence is, the less likely a target sequence will bepresent as a random occurrence in the database. The most preferredsequence length of a target sequence is from about 10 to 100 amino acidsor from about 30 to 300 nucleotide residues. However, it is wellrecognized that searches for commercially important fragments, such assequence fragments involved in gene expression and protein processing,may be of shorter length.

The term “a target structural motif” or “target motif” is defined hereinas any rationally selected sequence or combination of sequences in whichthe sequence(s) are chosen based on a three-dimensional configurationwhich is formed upon the folding of the target motif. There are avariety of target motifs known in the art. Protein target motifsinclude, but are not limited to, enzyme active sites and signalsequences, substrate and cofactor binding domains, transmembranedomains, and sites for post-translational modifications. Nucleic acidtarget motifs include, but are not limited to, promoter sequences,hairpin structures and inducible expression elements (protein bindingsequences), repeats, palindromes, dyad symmetries, and transcription andtranslation start and stop sites.

A variety of structural formats for the input and output means can beused to input and output the information in the computer-based systemsof the present invention. A preferred format for an output means ranksfragments of the Bacillus licheniformis or Bacillus clausii genomicsequences possessing varying degrees of homology to the target sequenceor target motif. Such presentation provides one skilled in the art witha ranking of sequences which contain various amounts of the targetsequence or target motif and identifies the degree of homology containedin the identified fragment.

A variety of comparing means can be used to compare a target sequence ortarget motif with the data storage means to identify sequence fragmentsof the Bacillus licheniformis and Bacillus clausii genomes. For example,implementing software which utilize the BLAST and BLAZE algorithms,described in Altschul et al., 1990, supra, may be used to identify openreading frames within the Bacillus licheniformis or Bacillus clausiigenome or the genomes of other organisms. A skilled artisan can readilyrecognize that any one of the publicly available homology searchprograms can be used as the search means for the computer-based systemsof the present invention. Suitable proprietary systems that may be knownto those of skill also may be employed in this regard.

Codon Usage Tables

The present invention further relates to methods for preparing asynthetic gene, comprising (a) generating a codon usage table based oncodons used in one or more open reading frames or portions thereof ofSEQ ID NO: 1, (b) constructing a synthetic gene or portion thereof thatcontains in place of one or more native codons one or more preferredcodons from the codon usage table, and (c) recovering the syntheticgene. In a preferred aspect, the codon usage table is Table 4 and/orTable 5.

The Bacillus licheniformis chromosomal sequence of SEQ ID NO: 1 orportions thereof can be used to generate codon usage tables to designsynthetic genes for their efficient heterologous expression in Bacilluslicheniformis host cells. The codon usage tables can be based on (1) thecodon used in all the open reading frames, (2) selected open readingframes, (3) fragments of the open reading frames, or (4) fragments ofselected open reading frames. With a codon usage table, synthetic genescan be designed with only the most preferred codon for each amino acid;with a number of common codons for each amino acid; or with the same ora similar statistical average of codon usages found in the table ofchoice.

The synthetic gene can be constructed using any method such assite-directed mutagenesis or PCR generated mutagenesis in accordancewith methods known in the art. Although, in principle, the modificationmay be performed in vivo, i.e., directly on the cell expressing thenucleotide sequence to be modified, it is preferred that themodification is performed in vitro.

The synthetic gene can be further modified by operably linking thesynthetic gene to one or more control sequences which direct theexpression of the coding sequence in a suitable host cell underconditions compatible with the control sequences using the methodsdescribed herein. Nucleic acid constructs, recombinant expressionvectors, and recombinant host cells comprising the synthetic gene canalso be prepared using the methods described herein.

The present invention also relates to methods for producing apolypeptide encoded by such a synthetic gene comprising (a) cultivatinga host cell comprising the synthetic gene under conditions conducive forproduction of the polypeptide; and (b) recovering the polypeptide.

The present invention is further described by the following exampleswhich should not be construed as limiting the scope of the invention.

EXAMPLES Example 1 Shotgun DNA Sequencing and Genome Assembly

The genome of the type strain Bacillus licheniformis ATCC 14580 wassequenced by a combination of the whole genome shotgun method describedby Wilson, R. K. and Mardis, E. R., 1997, In Genome Analysis: ALaboratory Manual, Vol. 1, eds. Birren, B., Green, E. D., Meyers, R. M.,and Roskams, J. (Cold Spring Harbor Press, Cold Spring Harbor, N.Y.),pp. 397-454, and fosmid end sequencing (Kim, U. J., Shizuya, H., deJong, P. J., Birren, B. and Simon, M. I., 1992, Nucleic Acids Res. 20:1083-1085; Longmire, J. L. and Brown, N. C., 2003, Biotechniques 35:50-54; Zhao, S., Malek, J., Mahairas, G., Fu, L., Nierman, W., Venter,J. C., and Adams, M. D., 2000, Genomics 63: 321-332).

Genomic DNA of Bacillus licheniformis ATCC 14580 was isolated using thefollowing method: A single colony was used to inoculate 20 ml of LBbroth (Davis, R. W., Botstein, D., and Roth, J. R. 1980, AdvancedBacterial Genetics, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.)in a sterile 125 ml Erlenmeyer flask. The culture was incubated at 37°C. overnight with agitation at 240 rpm. The resulting cells werecollected by centrifugation in a 45 ml Oak Ridge tube for 10 minutes at6000×g, and the cell pellet was resuspended in 5 ml of Tris-glucosebuffer (50 mM Tris-HCl, pH 8.0, 50 mM glucose, 10 mM EDTA). Lysozyme wasadded to a final concentration of 50 μg/ml and the suspension wasincubated in a 37° C. water bath for 25 minutes. Next, 200 μl of 10% SDSwas added and the tubes were gently inverted several times. Fivemilliliters of a second detergent mixture (1% Brij, 1% deoxycholate, 50mM EDTA, pH 7.5) was added, and the tubes were inverted several timeswhile incubating for 20 minutes at room temperature. An equal volume ofphenol:chloroform (1:1 v/v) was added and the tubes were inverted gentlyat room temperature for 20-30 minutes. The tubes were centrifuged for 20minutes at 12,000×g, 4° C. The top aqueous layer was carefully removedwith a wide-bore pipette and placed in a dean 45 ml Oak Ridge tube. Thephenol:chloroform extraction was repeated and 1/10 volume of 3 M sodiumacetate pH 5.2 was added to the aqueous layer. Two volumes of coldethanol were carefully layered on top and the DNA was spooled from thesolution onto a sterile glass rod. Spooled DNA was carefully rinsed in70% ethanol and resuspended in a suitable amount of TE buffer (10 mMTris-HCl, pH 8.0, 1 mM EDTA).

Plasmid libraries were constructed using randomly-sheared andBamHI-digested genomic DNA that was enriched for 2-3 kb fragments bypreparative agarose gel electrophoresis (Berka, R. M., Schneider, P.,Golightly, E. J., Brown, S. H., Madden, M., Brown, K. M., Halkier, T.,Mondorf, K., and Xu, F., 1997, Appl. Environ. Microbiol. 63: 3151-3157),Approximately 49,000 random clones were sequenced using dye-terminatorchemistry (Applied Biosystems, Foster City, Calif.) with ABI 377 and ABI3700 automated sequencers yielding approximately 6× coverage of thegenome. A combination of methods was employed for gap closure includingsequencing on fosmids (Kim, U. J., Shizuya, H., de Jong, P. J., Birren,B., and Simon, M. I., 1992, Nucleic Acids Res. 20: 1083-1085), primerwalking on selected clones, and PCR-amplified DNA fragments. Fosmidlibraries were constructed using a commercial kit from Epicentre(Madison, Wis.). Data from both ends of approximately 1975 fosmid cloneswith an average insert size of 40 kb were incorporated to aid invalidation of the final assembly. In total, the number of input readswas 62,685 with 78.6% of these incorporated into the final assembly.Sequences were base called using TraceTuner 2.0 (Paracel, Inc.,Pasadena, Calif.) and assembled using the Paracel Genome Assembler(Paracel, Inc., Pasadena, Calif.) with optimized parameters and thequality score set to >20. Phrap, Crossmatch, and Consed were used forsequence finishing (Gordon D., Abajian C., and Green P., 1998, GenomeRes. 8: 195-202).

Example 2 Identification and Annotation of Open Reading Frames (ORFs)

Protein coding regions in the assembled genome sequence data wereidentified using EasyGene (Larsen, T. S., and Krogh, A., 2003, BMCBioinformatics 4: 21), Glimmer (Delcher, A. L., Harmon, D., Kasif, S.,White, O. and Salzberg, S. L., 1999, Nucleic Acids Res. 27, 4636-4641),and FrameD (Schiex, T., Gouzy, J., Moisan, A. and de Oliveira, Y., 2003,Nucleic Acids Res. 31, 3738-3741). Only EasyGene gene models with anR-value of less than 2 and log-odds score greater than −10 were used.Predicted proteins were compared to the non-redundant database PIR-NREF(Wu, C. H., Huang, H., Arminski, L., Castro-Alvear, J., Chen, Y., Hu, Z.Z., Ledley, R. S., Lewis, K. C., Mewes, H. W., Orcutt, B. C., 2002,Nucleic Acids Research 30: 35-37) and the Bacillus subtilis genome(SubtilList) using BLASTP with an E-value threshold of 10⁻⁵.InterProScan was used to predict putative function. (Zdobnov, E. M. andApweiler, R., 2001, Bioinformatics 17, 847-848). The InterPro analysisincluded comparison to Pfam (Bateman, A., Coin, L., Durbin, R., Finn, R.D., Hollich, V., Griffiths-Jones, S., Khanna, A., Sonnhammer, E. L., etal., 2004, Nucleic Acids Res. 32, D138-D141), TIGRfam (Haft, D. J.,Selengut, J. D. and White, O., 2003, Nucleic Acids Res. 31: 371-373),Interpro (Apweiler, R., Attwood, T. K., Bairock, A., Bateman, A.,Birney, E., Biswas, M., Bucher, P., Cerutti, L., Corpet, F., Croning, M.D., et al., 2001, Nucleic Acids Res. 29: 37-40), signal peptideprediction using SignalP (Nielsen, H., Engelbrecht, J., Brunak, S., andvon Heijne, G., 1997, Protein Engineering 10: 1-6), and trans-membranedomain prediction using TMHMM (Krogh, A., Larsson, B., von Heijne, G.and Sonnhammer, E. L. L., 2000, J. Mol. Biol. 305, 567-580). These ORFswere assigned to functional categories based on the Cluster ofOrthologous Groups (COG) database (www.ncbi.nlm.nih.gov/COG) with manualverification as described (Tatusov, R. L., Koonin, E. V. and Lipman, D.J., 1997, Science 278: 631-637; Koonin, E. V. and Galperin, M. Y., 2002,Sequence—Evolution—Function: Computational Approaches in ComparativeGenomics (Kluwer, Boston)). Transfer RNA genes were identified usingtRNAscan-SE (Lowe, T. M. and Eddy, S. R., 1997, Nucleic Acids Res. 25:955-964).

Example 3 General Features of the Bacillus licheniformis Genome

The genome of Bacillus licheniformis ATCC 14580 was determined toconsist of a circular molecule of 4,222,336 bp with an average GCcontent of 46.2% (Table 2). No plasmids were found during the genomeanalysis, and none were found by agarose gel electrophoresis.

The genome contains 4208 predicted protein-coding genes with an averagesize of 873 bp, seven rRNA operons, and 81 RNA genes. Using acombination of several gene-finding algorithms 4208 protein coding ORFswere predicted. These ORFs constitute 87% of the genome and have anaverage length of 873 bp. Approximately 48% of the ORFs are encoded onone DNA strand and 52% on the other strand. Among the protein codingORFs, 3948 (94%) have significant similarity to proteins in PIR, and3187 of these gene models contain Interpro motifs and 2895 containprotein motifs found in PFAM. The number of hypothetical and conservedhypothetical proteins in the Bacillus licheniformis genome with hits inthe PIR database was 1318 (212 conserved hypothetical ORFs). Among thelist of hypothetical and conserved hypothetical ORFs, 683 (52%) haveprotein motifs contained in PFAM (148 conserved hypothetical ORFs).There are 72 tRNA genes representing all 20 amino acids and 7 rRNAoperons.

The likely origin of replication was identified by similarities toseveral features of Bacillus subtilis origin (Moriya, S., Fukuoka, T.,Ogasawara, N., and Yoshikawa, H., 1988, EMBO Journal 7: 2911-2917;Ogasawara, N., Nakai, S., and Yoshikawa, H., 1994, DNA Res. 1, 1-14;Kadoya, R., Hassan, A. K., Kasahara, Y., Ogasawara, N., and Moriya, S.,2002, Mol. Microbiol. 45: 73-87; Tosato, V., Gjuracic, K., Vlahovicek,K., Pongor, S., Danchin, A., and Bruschi, C. V., 2003, FEMS Microbiol.Lett. 218: 23-30). These included (a) colocalization of four genes(rpmH, dnaA, dnaN, and recF) found near the origin of the Bacillussubtilis chromosome, (b) GC nucleotide skew [(G−C)/(G+C)] analysis, and(c) the presence of multiple dnaA-boxes (Pedersen, A. G., Jensen, L. J.,Brunak, S., Staerfeldt, H. H., and Ussery, D. W., 2000, Mol. Biol. 299:907-930; Christensen, B. B., Atlung, T., and Hansen, F. G., 1999, J.Bacteriol. 181: 2683-2688; Majka, J., Jakimowicz, D., Messer, W.,Schrernpf, H., Lisowski, M., and Zakrzewska-Czerwińska, J., 1999, Eur.J. Biochem. 260: 325-335) and AT-rich sequences in the regionimmediately upstream of the dnaA gene. On the basis of theseobservations, a cytosine residue of the BstBI restriction site wasassigned between the rprnH and dnaA genes to be the first nucleotide ofthe Bacillus licheniformis genome. The replication termination site waslocalized near 2.02 Mb by GC skew analysis. This region lies roughlyopposite the origin of replication.

Unlike Bacillus subtilis, no apparent gene encoding a replicationterminator protein (rtp) was found in Bacillus licheniformis. TheBacillus halodurans genome also lacks an rtp function (Takami, H.,Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F.,Hirama, C., Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res.28; 4317-4331), and it seems likely that Bacillus subtilis acquired therip gene following its divergence from Bacillus halodurans and Bacilluslicheniformis.

Transposable elements and prophages. The genome of Bacilluslicheniformis ATCC 14580 was determined to contain nine identical copiesof a 1285 hp insertion sequence element termed IS3Bli1 (Lapidus, A.,Galleron, N., Andersen, J. T., Jørgensen, P. L. Ehrlich, S. D., andSorokin, A., 2002, FEMS Microbiol. Lett. 209: 23-30). This sequenceshares a number of features with other IS3 family elements includingdirect repeats of three to five bp, a ten bp left inverted repeat, and anine bp right inverted repeat. IS3Bli1 encodes two predicted overlappingORFs, designated orfA and orfB in relative translational reading framesof 0 and −1. The presence of a “slippery heptamer” motif, AAAAAAG,before the stop codon in orfA suggests that programmed translationalframeshifting occurs between these two ORFs, resulting in a single geneproduct (Farabaugh, P., 1996, Microbiol. Rev. 60: 103-134). The orfBgene product harbors the DD[35]E[7]K motif, a highly conserved patternamong insertion sequences. Eight of the IS3Bli1 elements lie inintergenic regions, and one interrupts the comP gene. In addition tothese insertion sequences, the genome encodes a putative transposasethat is most closely related (E=1.8e−11) to one identified in theThermoanaerobacter tengcongensis genome (Bao, Q., Tian, Y., Li, W., Xu,Z.; Xuan, Z., Hu, S., Dong, W., Yang, J., Chen, Y., Xue, Y., et al.,2002, Genome Res. 12: 689-700), however, similar genes are also found inthe chromosomes of Bacillus halodurans (Takami, H., Nakasone, K.,Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C.,Nakamura, Y., Ogasawara, N. et al., 2000, Nucleic Acids Res. 28:4317-4331), Oceanobacillus iheyensis (Takami, H., Takaki, Y., andUchiyama, I., 2002, Nucleic Acids Res. 30: 3927-3935.), Streptococcusagalactiae (Takahashi, S., Detrick, S., Whiting, A. A,Blaschke-Bonkowksy, A. J., Aoyagi, Y., Adderson, E. E., and Bohnsack, J.F., 2002, J. Infect. Dis. 186: 1034-1038), and Streptococcus pyogenes(Smoot, J. C., Barbian, K. D., Van Gompel, J. J., Smoot, L. M.,Chaussee, M. S., Sylva, G. L., Sturdevant, D. E., Ricklefs, S. M.,Porcella, S. F., Parkins, L. D., et al., 2002, Proc. Natl. Acad. Sci.U.S.A. 99: 4668-4673).

The presence of several bacteriophage lysogens or prophage-like elementswas revealed by Smith-Waterman comparisons to other bacterial genomesand by their AT-rich signatures. Prophage sequences, designated NZP1 andNZP3 (similar to PBSX and φ-105, respectively), were uncovered by notingthe presence of nearby genes encoding the large subunit of terminase, asignature protein that is highly conserved among prophages (Casjens, S.,2003, Mol. Microbiol. 49: 277-300). A terminase gene was not observed ina third putative prophage, termed NZP2 (similarity to SPP1), however,its absence may be the result of genome deterioration during evolution.Regions were observed in which the GC content is less than 39% usuallyencoded proteins that have no Bacillus subtilis orthologue and shareidentity only to hypothetical and conserved hypothetical genes. Two ofthese AT-rich segments correspond to the NZP2 and NZP3 prophages.

An isochore plot also revealed the presence of a region with anatypically high (62%) G+C content. This segment contains twohypothetical ORFs whose sizes (3831 and 2865 bp) greatly exceed the sizeof an average gene in Bacillus licheniformis. The first protein encodesa protein of 1277 amino acids for which Interpro predicted 16 collagentriple helix repeats, and the amino acid pattern TGATGPT is repeated 75times within the polypeptide. The second ORF is smaller, and encodes aprotein with 11 collagen triple helix repeats, and the same TGATGPTmotif recurs 56 times. Interestingly, the chromosomal region (19 kb)adjacent to these genes is clearly non-colinear with the Bacillussubtilis genome, and virtually all of the predicted ORFs arehypothetical or conserved hypothetical proteins. There are a number ofbacterial proteins listed in PIR that contain collagen triple helixrepeat regions including two from Mesorhizobium loti (accession numbersNF00607049 and NF00607035) and three from Bacillus cereus (accessionnumbers NF01692528, NF01269899, and NF01694666). These putativeorthologs share 53-76% amino acid sequence identity with theircounterparts in Bacillus licheniformis, although their functions areunknown.

Extracellular enzymes. In the Bacillus licheniformis genome, 689 of the4208 gene models have signal peptides as forecasted by SignalP(Identification of prokaryotic and eukaryotic signal peptides andprediction of their cleavage sites. Henrik Nielsen, Jacob Engelbrecht,Søren Brunak and Gunnar von Heijne, 1997, Protein Engineering 10: 1-6).Of these, 309 have no trans-membrane domain as predicted with TMHMM (A.Krogh, B. Larsson, G. von Heijne, and E. L. L. Sonnhammer, 2000, Journalof Molecular Biology 305: 567-580) and 134 are hypothetical or conservedhypothetical genes. Based on a manual examination of the remaining 175ORFs, at least 82 were determined to likely encode secreted proteins andenzymes. The sequence ID numbers for each of these genes are listed inTable 3.

Protein secretion, sporulation, and competence pathways. Kunst et al.(Kunst, F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G.,Azebedo, V., Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert,S., 1997, Nature 390: 249-256) listed 18 genes that play a major role inthe secretion of extracellular enzymes by Bacillus subtilis 168. Thislist includes several chaperonins, signal peptidases, components of thesignal recognition particle and protein translocase complexes. Thus, itis reasonable to expect that the central features of the secretoryapparatus are conserved in Bacillus subtilis and Bacillus licheniformis.

From the list of 139 sporulation genes tabulated by Kunst et al. (Kunst,F., Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V.,Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al.,1997, Nature 390: 249-256), all but six have obvious counterparts inBacillus licheniformis. These six exceptions (spsABCEFG) comprise anoperon involved in synthesis of spore coat polysaccharide in Bacillussubtilis. Additionally, the response regulator gene family (phrACEFGI)appears to have a low level of sequence conservation between Bacillussubtilis and Bacillus licheniformis.

Natural competence (the ability to take up and process exogenous DNA inspecific growth conditions) is a feature of few Bacillus licheniformisstrains (Gwinn, D. D. and Thorne, C. B., 1964, J. Bacteriol. 87:519-526). The reasons for variability in competence phenotype have notbeen explored at the genetic level, but the genome data offered severalpossible explanations. Although the type strain genome encodes all ofthe late competence functions ascribed in Bacillus subtilis (e.g., comC,comEFG operons, comK, mecA), it lacks an obvious comS gene, and the comPgene is punctuated by an insertion sequence element, suggesting that theearly stages of competence development have been preempted in Bacilluslicheniformis ATCC 14580. Whether these early functions can be restoredby introducing the corresponding genes from Bacillus subtilis isunknown. In addition to an apparent deficiency in DNA uptake, two Type Irestriction-modification systems were discovered that may alsocontribute diminished transformation efficiencies. These are distinctfrom the ydiOPS genes of Bacillus subtilis, and could participate indegradation of improperly modified DNA from heterologous hosts usedduring construction of recombinant expression vectors. Lastly, thesynthesis of a glutamyl polypeptide capsule has also been implicated asa potential barrier to transformation of Bacillus licheniformis strains(Thorne, C. B. and Stull, H. B., 1966, J. Bacteriol. 91: 1012-1020). Sixgenes were predicted (ywtABDEF and ywsC orthologues) that may beinvolved in the synthesis of this capsular material.

Antibiotics and secondary metabolites. Bacitracin is a cyclic peptideantibiotic that is synthesized non-ribosomally in Bacillus licheniformis(Katz, E. and Demain, A. L., 1977, Bacteriol. Rev. 41: 449-474). Whilethere is variation in the prevalence of bacitracin synthase genes inlaboratory strains of this species, one study suggested that up to 50%may harbor the bac operon (Ishihara, H., Takoh, M., Nishibayashi, R.,and Sato, A., 2002, Curr. Microbiol. 45: 18-23). The bac operon wasdetermined not to be present in the type strain (ATCC 14580) genome.Seemingly, the only non-ribosomal peptide synthase operon encoded by theBacillus licheniformis type strain genome is that which is responsiblefor lichenysin biosynthesis. Lichenysin structurally resembles surfactinfrom Bacillus subtilis (Grangemard, I., Wallach, J., Maget-Dana, R., andPeypoux, F., 2001, Appl. Biochem. Biotechnol. 90: 199-210), and theirrespective biosynthetic operons are highly similar. No Bacilluslicheniformis counterparts were found for the pps (plipastatin synthase)and polyketide synthase (pks) operons of Bacillus subtilis.Collectively, these two regions represent sizeable portions (80 kb and38 kb, respectively) of the chromosome in Bacillus subtilis, althoughthey are reportedly dispensable (Westers, H., Dorenbos, R., van Dijl, J.M., Kable, J., Flanagan, T., Devine, K. M., Jude, F., Séror, S. J.,Beekman, A. C., Darmon, E., 2003, Mol. Biol. Evol. 20: 2076-2090).Unexpectedly, a gene cluster was found encoding a lantibiotic andassociated processing and transport functions. This peptide of 69 aminoacids was designated as lichenicidin, and its closest known orthologueis mersacidin from Bacillus sp. strain HIL-Y85/54728 (Altena, K., Guder,A., Cramer, C., and Bierbaum, G., 2000, Appl. Environ. Microbiol. 66:2565-2571). Lantibiotics are ribosomally synthesized peptides that aremodified post-translationally so that the final molecules contain rarethioether amino acids such as lanthionine and/or methyl-lanthionine(Pag, U. and Sahl, H. G., 2002, Curr. Pharm. Des. 8: 815-833). Theseantimicrobial compounds have attracted much attention in recent years asmodels for the design of new antibiotics (Hoffmann, A., Pag, U.,Wiedemann, I., and Sahl, H. G., 2002, Farmaco, 57: 685-691).

Essential Genes. The gene models were also compared to the list ofessential genes in Bacillus subtilis (Kobayashi, K., Ehrlich, S. D.,Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K.,Ashikaga, S., Aymerch, S., Bessieres, P., 2003, Proc. Natl Acad. Sci.USA 100: 4678-4683). All the essential genes in Bacillus subtilis haveorthologues in Bacillus licheniformis, and most are present in a widerange of bacterial taxa (Pedersen, P. B., Bjørnvad, M. E., Rasmussen, M.D., and Petersen, J. N., 2002, Reg. Toxicol. Pharmacol. 36: 155-161).

Example 4 Comparison of Bacillus licheniformis Genome with Other Bacilli

VisualGenome software (Rational Genomics, San Francisco, Calif.) wasused for GC-skew analysis and global homology comparisons of theBacillus licheniformis, Bacillus subtilis, and Bacillus haloduransgenomes with pre-computed BLAST results stored in a local database. Inpairwise comparisons (E-score threshold of 10⁻⁵) 66% (2771/4208) of thepredicted Bacillus licheniformis ORFs have orthologs in Bacillussubtilis, and 55% (2321/4208) of the gene models are represented byorthologous sequences in Bacillus halodurans. Using a reciprocal BLASTPanalysis 1719 orthologs were found that are common to all three species(E-score threshold of 10⁻⁵).

As rioted by Lapidus et al. (Lapidus, A., Galleron, N., Andersen, J. T.,Jørgensen, P. L. Ehrlich, S. D., and Sorokin, A., 2002, FEMS Microbiol.Lett. 209: 23-30), there are broad regions of colinearity between thegenomes of Bacillus licheniformis and Bacillus subtilis. Lessconservation of genome organization exists between Bacilluslicheniformis and Bacillus halodurans, and inversion of one or morelarge genomic segments is evident. Clearly this supports previousfindings (Xu, D. and Côté, J. C., 2003, Internat. J. Syst. Evol.Microbiol. 53: 695-704) that Bacillus subtilis and Bacilluslicheniformis are phylogenetically and evolutionarily closer than eitherspecies is to Bacillus halodurans. However, a number of importantdifferences were also observed, both in the numbers and locations ofprophages and transposable elements and in a number of biochemicalpathways, which distinguish Bacillus licheniformis from Bacillussubtilis, including a region of more than 80 kb that comprises a clusterof polyketide synthase genes that are absent in Bacillus licheniformis.

Example 5 Codon Usage Tables

The evolution of codon bias, the unequal usage of synonomous codons, isthought to be due to natural selection for the use of preferred codonsthat match the most abundant species of isoaccepting tRNAs, resulting inincreased translational efficiency and accuracy. The practicalapplications for utilizing codon bias information include optimizingexpression of heterologous and mutant genes (Jiang and Mannervik, 1999,Protein Expression and Purification 15: 92-98), site-directedmutagenesis to derive variant polypeptides from a given gene (Wong etal., 1995, J. Immunol. 154: 3351-3358; Kaji, H. et al., 1999, J.Biochem. 126: 769-775), design and synthesis of synthetic genes(Libertini and Di Donato, 1992, Protein Engineering 5: 821-825; Fang etal., 2000, Biochem. 39: 15399-15409), and fine-tuning or reducing oftranslation efficiency of specific genes by introduction ofnon-preferred codons (Crombie, T. et al., 1992, J. Mol. Biol. 228: 7-12;Carlini and Stephan, 2003, Genetics 163: 239-243).

A codon usage table (Table 4) was generated from SEQ ID NO: 1 with CUSP,a software component of the EMBOSS package (Rice, Longden, and Bleasby,2000, EMBOSS: The European Molecular Biology Open Software Suite. Trendsin Genetics 16: 276-277) on all the predicted protein-coding genes ofthe Bacillus licheniformis chromosome. CUSP read the coding sequencesand calculated the codon frequency table shown in Table 4.

A codon usage table (Table 5) was also generated based on the signalpeptides of the 82 extracellular proteins described in Example 3.

TABLE 1 Predicted functions Bacillus subtilis homolog SEQ ID (Gene NO.Description UniRef Accession No. Organism Name) 2 Chromosomalreplication initiator UniRef100_P05648 Bacillus subtilis DnaA proteindnaA [Bacillus subtilis] 3 DNA polymerase III, beta chainUniRef100_P05649 Bacillus subtilis DnaN [Bacillus subtilis] 4 5 DNAreplication and repair protein recF UniRef100_P05651 Bacillus subtilisRecF [Bacillus subtilis] 6 7 DNA gyrase subunit B [Bacillus subtilis]UniRef100_P05652 Bacillus subtilis GyrB 8 DNA gyrase subunit A [Bacillussubtilis] UniRef100_P05653 Bacillus subtilis GyrA 9 YaaC 10Inosine-5′-monophosphate UniRef100_P21879 Bacillus subtilis GuaBdehydrogenase [Bacillus subtilis] 11 D-alanyl-D-alanine carboxypeptidaseUniRef100_P08750 Bacillus subtilis DacA precursor [Bacillus subtilis] 12Pyridoxine biosynthesis protein pdx1 UniRef100_P37527 Bacillus subtilisYaaD [Bacillus subtilis] 13 Hypothetical UPF0030 protein yaaEUniRef100_P37528 Bacillus subtilis YaaE [Bacillus subtilis] 14Seryl-tRNA synthetase [Bacillus UniRef100_P37464 Bacillus subtilis SerSsubtilis] 15 Glycerate kinase [Bacillus subtilis] UniRef100_P42100Bacillus subtilis YxaA 16 H+/gluconate symporter [VibrioUniRef100_Q7MHW6 Vibrio vulnificus YuiF vulnificus] 17 Sugar diacidutilization regulator [Vibrio UniRef100_Q8DBZ9 Vibrio vulnificus YsfBvulnificus] 18 Hypothetical protein [Bacillus UniRef100_Q6HH43 Bacillusthuringiensis thuringiensis] 19 Hypothetical protein yaaF [BacillusUniRef100_P37529 Bacillus subtilis Dck subtilis] 20 Hypothetical proteinyaaG [Bacillus UniRef100_P37530 Bacillus subtilis Dgk subtilis] 21Hypothetical protein yaaH [Bacillus UniRef100_P37531 Bacillus subtilisYaaH subtilis] 22 Hypothetical protein yaaI [Bacillus UniRef100_P37532Bacillus subtilis YaaI subtilis] 23 YaaJ 24 DnaX 25 Hypothetical UPF0133protein yaaK UniRef100_P24281 Bacillus subtilis YaaK [Bacillus subtilis]26 Recombination protein recR [Bacillus UniRef100_P24277 Bacillussubtilis RecR subtilis] 27 Hypothetical protein yaaL [BacillusUniRef100_P37533 Bacillus subtilis subtilis] 28 Sigma-K factorprocessing regulatory UniRef100_P24282 Bacillus subtilis protein BOFA[Bacillus subtilis] 29 CsfB protein [Bacillus subtilis] UniRef100_P37534Bacillus subtilis 30 XpaC protein [Bacillus subtilis] UniRef100_P37467Bacillus subtilis XpaC 31 Hypothetical protein yaaN [BacillusUniRef100_P37535 Bacillus subtilis YaaN subtilis] 32 YaaO 33 Thymidylatekinase [Bacillus subtilis] UniRef100_P37537 Bacillus subtilis Tmk 34Hypothetical protein yaaQ [Bacillus UniRef100_P37538 Bacillus subtilisYaaQ subtilis] 35 36 DNA polymerase III, delta′ subunit UniRef100_P37540Bacillus subtilis HolB [Bacillus subtilis] 37 Hypothetical protein yaaT[Bacillus UniRef100_P37541 Bacillus subtilis YaaT subtilis] 38Hypothetical protein yabA [Bacillus UniRef100_P37542 Bacillus subtilisYabA subtilis] 39 Hypothetical protein yabB [Bacillus UniRef100_P37543Bacillus subtilis YabB subtilis] 40 Hypothetical UPF0213 protein yazAUniRef100_O31414 Bacillus subtilis [Bacillus subtilis] 41 HypotheticalUPF0011 protein yabC UniRef100_P37544 Bacillus subtilis YabC [Bacillussubtilis] 42 Transition state regulatory protein abrB UniRef100_P08874Bacillus subtilis [Bacillus subtilis] 43 Methionyl-tRNA synthetase[Bacillus UniRef100_P37465 Bacillus subtilis MetS subtilis] 44 Putativedeoxyribonuclease yabD UniRef100_P37545 Bacillus subtilis YabD [Bacillussubtilis] 45 YabE 46 Hypothetical protein yabF [BacillusUniRef100_P37547 Bacillus subtilis RnmV subtilis] 47 Dimethyladenosinetransferase (EC UniRef100_P37468 Bacillus subtilis KsgA 2.1.1.—)(S-adenosylmethionine-6-N′,N′- adenosyl(rRNA) dimethyltransferase)[Bacillus subtilis] 48 Hypothetical protein yabG [BacillusUniRef100_P37548 Bacillus subtilis YabG subtilis] 49 Veg protein[Bacillus subtilis] UniRef100_P37466 Bacillus subtilis 50 SspF protein[Bacillus subtilis] UniRef100_P37549 Bacillus subtilis 51 IspE 52 Puroperon repressor [Bacillus subtilis] UniRef100_P37551 Bacillus subtilisPurR 53 UPF0076 protein yabJ [Bacillus subtilis] UniRef100_P37552Bacillus subtilis YabJ 54 Stage V sporulation protein G [BacillusUniRef100_P28015 Bacillus subtilis subtilis] 55 Bifunctional gcaDprotein (TMS protein) UniRef100_P14192 Includes: UDP- GcaD [Includes:UDP-N-acetylglucosamine N- pyrophosphorylase (EC 2.7.7.23) (N-acetylglucosamine acetylglucosamine-1-phosphate pyrophosphorylaseuridyltransferase); Glucosamine-1- (EC 2.7.7.23) phosphateN-acetyltransferase (EC (N- 2.3.1.157)] [Bacillus subtilis]acetylglucosamine- 1-phosphate uridyltransferase); Glucosamine-1-phosphate N- acetyltransferase (EC 2.3.1.157) 56 Ribose-phosphatepyrophosphokinase UniRef100_P14193 Bacillus subtilis Prs [Bacillussubtilis] 57 General stress protein ctc [Bacillus UniRef100_P14194Bacillus subtilis Ctc subtilis] 58 Peptidyl-tRNA hydrolase [BacillusUniRef100_P37470 Bacillus subtilis SpoVC subtilis] 59 Hypotheticalprotein yabK [Bacillus UniRef100_P37553 Bacillus subtilis subtilis] 60Transcription-repair coupling factor UniRef100_P37474 Bacillus subtilisMfd [Bacillus subtilis] 61 Stage V sporulation protein T [BacillusUniRef100_P37554 Bacillus subtilis SpoVT subtilis] 62 Hypotheticalprotein yabM [Bacillus UniRef100_P37555 Bacillus subtilis YabM subtilis]63 Hypothetical protein yabN [Bacillus UniRef100_P37556 Bacillussubtilis YabN subtilis] 64 Hypothetical protein yabO [BacillusUniRef100_P37557 Bacillus subtilis subtilis] 65 Hypothetical proteinyabP [Bacillus UniRef100_P37558 Bacillus subtilis YabP subtilis] 66Hypothetical protein yabQ [Bacillus UniRef100_P37559 Bacillus subtilisYabQ subtilis] 67 Cell division protein divlC [Bacillus UniRef100_P37471Bacillus subtilis DivlC subtilis] 68 Hypothetical protein yabR [BacillusUniRef100_P37560 Bacillus subtilis YabR subtilis] 69 Stage IIsporulation protein E [Bacillus UniRef100_P37475 Bacillus subtilisSpoIIE subtilis] 70 Hypothetical protein yabS [Bacillus UniRef100_P37561Bacillus subtilis YabS subtilis] 71 Probable serine/threonine-proteinUniRef100_P37562 Bacillus subtilis YabT kinase yabT [Bacillus subtilis]72 Hypothetical UPF0072 protein yacA UniRef100_P37563 Bacillus subtilisYacA [Bacillus subtilis] 73 Hypoxanthine-guanine UniRef100_P37472Bacillus subtilis HprT phosphoribosyltransferase [Bacillus subtilis] 74Cell division protein ftsH homolog UniRef100_P37476 Bacillus subtilisFtsH [Bacillus subtilis] 75 Putative 32 kDa replication proteinUniRef100_Q9F985 Bacillus YacB [Bacillus stearothermophilus]stearothermophilus 76 33 kDa chaperonin [Bacillus subtilis]UniRef100_P37565 Bacillus subtilis YacC 77 YacD 78 CysK 79Para-aminobenzoate synthase UniRef100_P28820 Bacillus subtilis PabBcomponent I [Bacillus subtilis] 80 Para-aminobenzoate/anthranilateUniRef100_P28819 Includes: Para- PabA synthase glutamineamidotransferase aminobenzoate component II [Includes: Para- synthaseaminobenzoate synthase glutamine glutamine amidotransferase component II(EC amidotransferase 6.3.5.8) (ADC synthase); Anthranilate component IIsynthase component II (EC 4.1.3.27)] (EC 6.3.5.8) [Bacillus subtil (ADCsynthase); Anthranilate synthase component II (EC 4.1.3.27) 81Aminodeoxychorismate lyase [Bacillus UniRef100_P28821 Bacillus subtilisPabC subtilis] 82 Dihydropteroate synthase [Bacillus UniRef100_P28822Bacillus subtilis SuI subtilis] 83 Dihydroneopterin aldolase [BacillusUniRef100_P28823 Bacillus subtilis FolB subtilis] 842-amino-4-hydroxy-6- UniRef100_P29252 Bacillus subtilis FolKhydroxymethyldihydropteridine pyrophosphokinase [Bacillus subtilis] 85YazB protein [Bacillus subtilis] UniRef100_O31417 Bacillus subtilis 86Probable tRNA-dihydrouridine synthase UniRef100_P37567 Bacillus subtilisYacF 1 [Bacillus subtilis] 87 Lysyl-tRNA synthetase [BacillusUniRef100_P37477 Bacillus subtilis LysS subtilis] 88 Transcriptionalregulator ctsR [Bacillus UniRef100_P37568 Bacillus subtilis CtsRsubtilis] 89 Hypothetical protein yacH [Bacillus UniRef100_P37569Bacillus subtilis McsA subtilis] 90 Hypothetical ATP:guanidoUniRef100_P37570 Bacillus subtilis McsB phosphotransferase yacI[Bacillus subtilis] 91 Negative regulator of genetic UniRef100_P37571Bacillus subtilis ClpC competence clpC/mecB [Bacillus subtilis] 92 RadA93 Hypothetical protein yacK [Bacillus UniRef100_P37573 Bacillussubtilis YacK subtilis] 94 Hypothetical protein yacL [BacillusUniRef100_Q06754 Bacillus subtilis YacL subtilis] 952-C-methyl-D-erythritol 4-phosphate UniRef100_Q06755 Bacillus subtilisYacM cytidylyltransferase [Bacillus subtilis] 96 2-C-methyl-D-erythritol2,4- UniRef100_Q06756 Bacillus subtilis YacN cyclodiphosphate synthase[Bacillus subtilis] 97 Glutamyl-tRNA synthetase [BacillusUniRef100_P22250 Bacillus subtilis GltX subtilis] 98 Serineacetyltransferase [Bacillus UniRef100_Q06750 Bacillus subtilis CysEsubtilis] 99 Cysteinyl-tRNA synthetase [Bacillus UniRef100_Q06752Bacillus subtilis CysS subtilis] 100 YazC protein [Bacillus subtilis]UniRef100_O31418 Bacillus subtilis YazC 101 Hypothetical tRNA/rRNAUniRef100_Q06753 Bacillus subtilis YacO methyltransferase yacO [Bacillussubtilis] 102 Hypothetical protein yacP [Bacillus UniRef100_P37574Bacillus subtilis YacP subtilis] 103 RNA polymerase sigma-H factorUniRef100_P17869 Bacillus subtilis SigH [Bacillus subtilis] 104Preprotein translocase secE subunit UniRef100_Q06799 Bacillus subtilis[Bacillus subtilis] 105 NusG 106 50S ribosomal protein L11 [BacillusUniRef100_Q06796 Bacillus subtilis RplK subtilis] 107 50S ribosomalprotein L1 [Bacillus UniRef100_Q06797 Bacillus subtilis RplA subtilis]108 50S ribosomal protein L10 [Bacillus UniRef100_P42923 Bacillussubtilis RplJ subtilis] 109 50S ribosomal protein L7/L12 [BacillusUniRef100_P02394 Bacillus subtilis RplL subtilis] 110 Hypotheticalprotein ybxB [Bacillus UniRef100_P37872 Bacillus subtilis YbxB subtilis]111 DNA-directed RNA polymerase beta UniRef100_P37870 Bacillus subtilisRpoB chain [Bacillus subtilis] 112 DNA-directed RNA polymerase beta′UniRef100_P37871 Bacillus subtilis RpoC chain [Bacillus subtilis] 113Putative ribosomal protein L7Ae-like UniRef100_P46350 Bacillus subtilis[Bacillus subtilis] 114 30S ribosomal protein S12 [BacillusUniRef100_P21472 Bacillus subtilis RpsL subtilis] 115 30S ribosomalprotein S7 [Bacillus UniRef100_P21469 Bacillus subtilis RpsG subtilis]116 Elongation factor G [Bacillus subtilis] UniRef100_P80868 Bacillussubtilis FusA 117 Elongation factor Tu [Bacillus subtilis]UniRef100_P33166 Bacillus subtilis TufA 118 30S ribosomal protein S10[Bacillus UniRef100_Q9Z9L5 Bacillus RpsJ halodurans] halodurans 119 50Sribosomal protein L3 [Bacillus UniRef100_P42920 Bacillus subtilis RplCsubtilis] 120 50S ribosomal protein L4 [Bacillus UniRef100_P42921Bacillus subtilis RplD subtilis] 121 50S ribosomal protein L23 [BacillusUniRef100_P42924 Bacillus subtilis subtilis] 122 50S ribosomal proteinL2 [Bacillus UniRef100_P42919 Bacillus subtilis RplB subtilis] 123 30Sribosomal protein S19 [Bacillus UniRef100_P21476 Bacillus subtilissubtilis] 124 50S ribosomal protein L22 [Bacillus UniRef100_P42060Bacillus subtilis RplV subtilis] 125 30S ribosomal protein S3 [BacillusUniRef100_P21465 Bacillus subtilis RpsC subtilis] 126 50S ribosomalprotein L16 [Bacillus UniRef100_P14577 Bacillus subtilis RplP subtilis]127 50S ribosomal protein L29 [Bacillus UniRef100_P12873 Bacillussubtilis subtilis] 128 30S ribosomal protein S17 [BacillusUniRef100_P12874 Bacillus subtilis subtilis] 129 130 50S ribosomalprotein L24 [Bacillus UniRef100_P12876 Bacillus subtilis RplX subtilis]131 50S ribosomal protein L5 [Bacillus UniRef100_P12877 Bacillussubtilis RplE subtilis] 132 Ribosomal protein S14 [Bacillus cereusUniRef100_Q63H77 Bacillus cereus ZK ZK] 133 30S ribosomal protein S8[Bacillus UniRef100_P12879 Bacillus subtilis RpsH subtilis] 134 50Sribosomal protein L6 [Bacillus UniRef100_P46898 Bacillus subtilis RplFsubtilis] 135 50S ribosomal protein L18 [Bacillus UniRef100_P46899Bacillus subtilis RplR subtilis] 136 30S ribosomal protein S5 [BacillusUniRef100_P21467 Bacillus subtilis RpsE subtilis] 137 50S ribosomalprotein L30 [Bacillus UniRef100_P19947 Bacillus subtilis subtilis] 13850S ribosomal protein L15 [Bacillus UniRef100_P19946 Bacillus subtilisRplO subtilis] 139 SecY 140 Adenylate kinase [Bacillus subtilis]UniRef100_P16304 Bacillus subtilis Adk 141 Methionine aminopeptidase[Bacillus UniRef100_P19994 Bacillus subtilis Map subtilis] 142 C-125initiation factor IF-I, RNA UniRef100_O50629 Bacillus haloduranspolymerase alpha subunit and ribosomal proteins, partial and completecds [Bacillus halodurans] 143 Translation initiation factor IF-1UniRef100_P20458 Bacillus subtilis [Bacillus subtilis] 144 145 RpsM 14630S ribosomal protein S11 [Bacillus UniRef100_P04969 Bacillus subtilisRpsK subtilis] 147 DNA-directed RNA polymerase alpha UniRef100_P20429Bacillus subtilis RpoA chain [Bacillus subtilis] 148 50S ribosomalprotein L17 [Bacillus UniRef100_P20277 Bacillus subtilis RplQ subtilis]149 Hypothetical ABC transporter ATP- UniRef100_P40735 Bacillus subtilisYbxA binding protein ybxA [Bacillus subtilis] 150 Hypothetical proteinorf5 [Bacillus UniRef100_P70970 Bacillus subtilis YbaE subtilis] 151YbaF protein [Bacillus subtilis] UniRef100_P70972 Bacillus subtilis YbaF152 tRNA pseudouridine synthase A UniRef100_P70973 Bacillus subtilisTruA [Bacillus subtilis] 153 50S ribosomal protein L13 [BacillusUniRef100_P70974 Bacillus subtilis RplM subtilis] 154 30S ribosomalprotein S9 [Bacillus UniRef100_P21470 Bacillus subtilis RpsI subtilis]155 Hypothetical protein [Bacillus cereus] UniRef100_Q737T6 Bacilluscereus YizA 156 Hypothetical protein ybaK [Bacillus UniRef100_P50862Bacillus subtilis YbaK subtilis] 157 Germination-specific N-UniRef100_P50864 Bacillus subtilis CwlD acetylmuramoyl-L-alanine amidase[Bacillus subtilis] 158 YbaL 159 Spore germination protein gerDUniRef100_P16450 Bacillus subtilis GerD precursor [Bacillus subtilis]160 KinB signaling pathway activation UniRef100_P16449 Bacillus subtilisKbaA protein [Bacillus subtilis] 161 Hypothetical protein ybaN precursorUniRef100_P50865 Bacillus subtilis YbaN [Bacillus subtilis] 162Penicillin-binding protein [Bacillus UniRef100_O31773 Bacillus subtilisPbpX subtilis] 163 164 Hypothetical protein ybaS [BacillusUniRef100_P55190 Bacillus subtilis YbaS subtilis] 165 Hypotheticalprotein yxaJ [Bacillus UniRef100_P42109 Bacillus subtilis YxaJ subtilis]166 Phenazine biosynthetic protein UniRef100_Q9HHG6 Halobacterium YfhB[Halobacterium sp.] sp. 167 Hypothetical protein ybbC precursorUniRef100_P40407 Bacillus subtilis YbbC [Bacillus subtilis] 168Hypothetical lipoprotein ybbD precursor UniRef100_P40406 Bacillussubtilis YbbD [Bacillus subtilis] 169 Hypothetical UPF0214 protein ybbEUniRef100_O05213 Bacillus subtilis YbbE precursor [Bacillus subtilis]170 YbbF protein [Bacillus subtilis] UniRef100_Q797S1 Bacillus subtilisYbbF 171 Putative HTH-type transcriptional UniRef100_Q45581 Bacillussubtilis YbbH regulator ybbH [Bacillus subtilis] 172 Hypotheticalprotein ybbI [Bacillus UniRef100_Q45582 Bacillus subtilis YbbI subtilis]173 Hypothetical protein ybbK [Bacillus UniRef100_Q45584 Bacillussubtilis YbbK subtilis] 174 Arginase [Bacillus caldovelox]UniRef100_P53608 Bacillus RocF caldovelox 175 RNA polymerase sigmafactor sigW UniRef100_Q45585 Bacillus subtilis SigW [Bacillus subtilis]176 YbbM protein [Bacillus subtilis] UniRef100_Q45588 Bacillus subtilisYbbM 177 YbbP protein [Bacillus subtilis] UniRef100_Q45589 Bacillussubtilis YbbP 178 YbbR protein [Bacillus subtilis] UniRef100_O34659Bacillus subtilis YbbR 179 YbbT protein [Bacillus subtilis]UniRef100_O34824 Bacillus subtilis YbbT 180Glucosamine--fructose-6-phosphate UniRef100_P39754 isomerizing GlmSaminotransferase [isomerizing] [Bacillus subtilis] 181 182 Hypotheticalprotein [Bacillus UniRef100_Q6HH47 Bacillus thuringiensis thuringiensis]183 UPI00003CBF92 UniRef100 entry UniRef100_UPI00003CBF92 YtrB 184Transcriptional regulator [Bacillus UniRef100_Q9KF35 Bacillus YtrAhalodurans] halodurans 185 186 YbcL protein [Bacillus subtilis]UniRef100_O34663 Bacillus subtilis YbcL 187 BH0186 protein [Bacillushalodurans] UniRef100_Q9KGB8 Bacillus halodurans 188 ABC transporter[Bacillus halodurans] UniRef100_Q9KEY6 Bacillus YvcC halodurans 189Hypothetical protein ywbO [Bacillus UniRef100_P39598 Bacillus subtilisYwbO subtilis] 190 191 BH0695 protein [Bacillus halodurans]UniRef100_Q9KF04 Bacillus halodurans 192 Aminoglycoside6-adenylyltranserase UniRef100_Q6V4U6 Enterococcus AadK [Enterococcusfaecium] faecium 193 Hypothetical conserved protein UniRef100_Q8ERX2Oceanobacillus iheyensis [Oceanobacillus iheyensis] 194 YfnB [Bacillussubtilis] UniRef100_O06480 Bacillus subtilis YfnB 195 Hypotheticaltransport protein ybxG UniRef100_P54425 Bacillus subtilis YbxG [Bacillussubtilis] 196 Mg(2+)/citrate complex secondary UniRef100_P55069 Bacillussubtilis CitM transporter [Bacillus subtilis] 197 YflP protein [Bacillussubtilis] UniRef100_O34439 Bacillus subtilis YflP 198 CitT 199 Sensorprotein citS [Bacillus subtilis] UniRef100_O34427 Bacillus subtilis CitS200 Transcriptional regulator UniRef100_Q8RB37 Thermoanaerobacter YwfK[Thermoanaerobacter tengcongensis] tengcongensis 201 Complete genome;segment 8/17 UniRef100_Q7N4W7 Photorhabdus YwfE [Photorhabdusluminescens] luminescens 202 Multidrug resistance protein B [BacillusUniRef100_Q81AF0 Bacillus cereus YqjV cereus] 203 Sigma-G-dependentsporulation UniRef100_P54379 Bacillus subtilis specific SASP protein[Bacillus subtilis] 204 Hypothetical protein ybxH [BacillusUniRef100_P54426 Bacillus subtilis subtilis] 205 YbyB protein [Bacillussubtilis] UniRef100_O31441 Bacillus subtilis 206 Hypothetical proteinyyaL [Bacillus UniRef100_P37512 Bacillus subtilis YyaL subtilis] 207Hypothetical protein yyaO [Bacillus UniRef100_P37509 Bacillus subtilissubtilis] 208 Threonyl-tRNA synthetase 2 [Bacillus UniRef100_P18256Bacillus subtilis ThrZ subtilis] 209 210 YttB 211 Hypothetical protein[Acinetobacter sp.] UniRef100_Q6FDN0 Acinetobacter sp. 212 HypotheticalUPF0053 protein yrkA UniRef100_P54428 Bacillus subtilis YrkA [Bacillussubtilis] 213 YdeG 214 YbaJ 215 216 YqeW 217 YubF protein [Bacillussubtilis] UniRef100_O32082 Bacillus subtilis 218 YbfF 219 Hypotheticalprotein [Bacteroides UniRef100_Q64UG6 Bacteroides fragilis fragilis] 220Hypothetical transport protein ybhF UniRef100_O31448 Bacillus subtilisYbfH [Bacillus subtilis] 221 YbfI protein [Bacillus subtilis]UniRef100_O31449 Bacillus subtilis YbfI 222 223 UPI00003CBC98 UniRef100entry UniRef100_UPI00003CBC98 224 225 Oxidoreductase [Lactococcuslactis] UniRef100_Q9CFC5 Lactococcus lactis 226 Methyl-acceptingchemotaxis protein UniRef100_Q8EST3 Oceanobacillus YvaQ [Oceanobacillusiheyensis] iheyensis 227 D-xylose-binding protein UniRef100_O68456Thermoanaerobacter RbsB [Thermoanaerobacter ethanolicus] ethanolicus 228Hypothetical protein OB0544 UniRef100_Q8ESS4 Oceanobacillus YomI[Oceanobacillus iheyensis] iheyensis 229 Hypothetical protein [Bacilluscereus] UniRef100_Q81IR4 Bacillus cereus 230 Streptogramin B lactonaseUniRef100_O87275 Staphylococcus cohnii [Staphylococcus cohnii] 231Hypothetical protein ycbP [Bacillus UniRef100_P42248 Bacillus subtilisYcbP subtilis] 232 YbgF protein [Bacillus subtilis] UniRef100_O31462Bacillus subtilis YbgF 233 YbgG protein [Bacillus subtilis]UniRef100_O31463 Bacillus subtilis YbgG 234 235 PTS system,n-acetylglucosamine- UniRef100_Q9KF24 Bacillus NagP specific enzyme II,ABC component halodurans [Bacillus halodurans] 236 UPI00003CBDBCUniRef100 entry UniRef100_UPI00003CBDBC Mta 237 Hypothetical protein[Bacillus UniRef100_Q6HIW0 Bacillus YdfS thuringiensis] thuringiensis238 Carbonic anhydrase [Methanosarcina UniRef100_Q8PSJ1 MethanosarcinaYtoA mazei] mazei 239 Hypothetical protein yjgA [BacillusUniRef100_Q6HHU2 Bacillus YjgA thuringiensis] thuringiensis 240 Aminoacid carrier protein [Bacillus UniRef100_Q6HGU4 Bacillus YbgHthuringiensis] thuringiensis 241 Probable glutaminase ybgJ [BacillusUniRef100_O31465 Bacillus subtilis YbgJ subtilis] 242 Two-componentsensor kinase ycbA UniRef100_Q81BN8 Bacillus cereus YcbA [Bacilluscereus] 243 Hypothetical sensory transduction UniRef100_P40759 Bacillussubtilis YcbB protein ycbB [Bacillus subtilis] 244 YoaA 245 YbxI 246 247Beta-lactamase precursor (EC 3.5.2.6) UniRef100_P00808 Contains: LargePenP (Penicillinase) [Contains: Large exopenicillinase;exopenicillinase; Small Small exopenicillinase] [Bacillus licheniformis]exopenicillinase 248 Alkaline phosphatase D precursor UniRef100_P42251Bacillus subtilis PhoD [Bacillus subtilis] 249 250 Hypothetical proteinycbT [Bacillus UniRef100_P42252 Bacillus subtilis TatCD subtilis] 251YcbC 252 Probable aldehyde dehydrogenase UniRef100_P42236 Bacillussubtilis YcbD ycbD [Bacillus subtilis] 253 Probable glucaratetransporter [Bacillus UniRef100_P42237 Bacillus subtilis YcbE subtilis]254 Probable glucarate dehydratase UniRef100_P42238 Bacillus subtilisYcbF [Bacillus subtilis] 255 Hypothetical transcriptional regulatorUniRef100_P42239 Bacillus subtilis YcbG ycbG [Bacillus subtilis] 256Probable D-galactarate dehydratase UniRef100_P42240 Bacillus subtilisYcbH [Bacillus subtilis] 257 Hypothetical sensory transductionUniRef100_P42244 Bacillus subtilis YcbL protein ycbL [Bacillus subtilis]258 Sensor histidine kinase [Bacillus cereus UniRef100_Q633Q6 Bacilluscereus ZK ZK] 259 Hypothetical ABC transporter ATP- UniRef100_P42246Bacillus subtilis YcbN binding protein ycbN [Bacillus subtilis] 260Hypothetical protein ycbO [Bacillus UniRef100_P42247 Bacillus subtilisYcbO subtilis] 261 YcbO 262 YfnK [Bacillus subtilis] UniRef100_O06490Bacillus subtilis YetN 263 Hypothetical protein ybdO [BacillusUniRef100_O31437 Bacillus subtilis YbdO subtilis] 264 Hypotheticalprotein ycbJ [Bacillus UniRef100_P42242 Bacillus subtilis YcbJ subtilis]265 Hypothetical protein ywhA [Bacillus UniRef100_P70993 Bacillussubtilis YwhA subtilis] 266 YdaB 267 AII0778 protein [Anabaena sp.]UniRef100_Q8YYR8 Anabaena sp. 268 BH1298 protein [Bacillus halodurans]UniRef100_Q9KDB5 Bacillus YbdN halodurans 269 BH1299 protein [Bacillushalodurans] UniRef100_Q9KDB4 Bacillus halodurans 270 Transcriptionalactivator tipA, putative UniRef100_Q81XN3 Bacillus Mta [Bacillusanthracis] anthracis 271 272 Transcriptional regulator, PadR familyUniRef100_Q81BZ0 Bacillus cereus [Bacillus cereus] 273 Hypotheticalprotein [Bacillus cereus UniRef100_Q639U7 Bacillus cereus ZK ZK] 274Tryptophan RNA-binding attenuator UniRef100_O31466 Bacillus subtilisprotein-inhibitory protein [Bacillus subtilis] 275 Hypotheticaltransport protein ycbK UniRef100_P42243 Bacillus subtilis YcbK [Bacillussubtilis] 276 YczC 277 Hypothetical protein yccF [BacillusUniRef100_O34478 Bacillus subtilis YccF subtilis] 278 SpaF [Bacillussubtilis] UniRef100_Q45404 Bacillus subtilis YhcH 279 SpaE [Bacillussubtilis] UniRef100_O52853 Bacillus subtilis 280 Putative SpaG [Bacillussubtilis] UniRef100_Q93GG9 Bacillus subtilis 281 Subtilin biosynthesisregulatory protein UniRef100_P33112 Bacillus subtilis YycF spaR[Bacillus subtilis] 282 Putative histidine kinase [BacillusUniRef100_Q93GG7 Bacillus subtilis ResE subtilis] 283 UPI00003CA401UniRef100 entry UniRef100_UPI00003CA401 YtlI 284 YusQ protein [Bacillussubtilis] UniRef100_O32183 Bacillus subtilis YusQ 285 Complete genome;segment 6/17 UniRef100_Q7N6K9 Photorhabdus YusR [Photorhabdusluminescens] luminescens 286 Methyltransferase [BacillusUniRef100_Q6HK82 Bacillus thuringiensis thuringiensis] 287 Hypotheticallipoprotein ycdA precursor UniRef100_O34538 Bacillus subtilis YcdA[Bacillus subtilis] 288 UPI00003CB481 UniRef100 entryUniRef100_UPI00003CB481 YcgA 289 Hypothetical conserved proteinUniRef100_Q8ESQ2 Oceanobacillus YtnL [Oceanobacillus iheyensis]iheyensis 290 Hypothetical conserved protein UniRef100_Q8ET83Oceanobacillus YvbK [Oceanobacillus iheyensis] iheyensis 291 GatA 292Oligoendopeptidase F, putative UniRef100_Q81JJ8 Bacillus YjbG [Bacillusanthracis] anthracis 293 294 UPI00003CC42D UniRef100 entryUniRef100_UPI00003CC42D PbpX 295 Hypothetical protein ysfD [BacillusUniRef100_P94534 Bacillus subtilis YsfD subtilis] 296 Hypotheticalprotein ysfC [Bacillus UniRef100_P94535 Bacillus subtilis YsfC subtilis]297 PTS system, cellobiose-specific UniRef100_Q9KEE3 Bacillus haloduransenzyme II, B component [Bacillus halodurans] 298 PTS system,cellobiose-specific UniRef100_Q9KEE2 Bacillus LicA enzyme II, Acomponent [Bacillus halodurans halodurans] 299 PTS system,cellobiose-specific UniRef100_Q8EP43 Oceanobacillus YwbA enzyme II, Ccomponent iheyensis [Oceanobacillus iheyensis] 3006-phospho-beta-glucosidase [Bacillus UniRef100_Q9KEE0 Bacillus LicHhalodurans] halodurans 301 Transcriptional regulator [BacillusUniRef100_Q9KED8 Bacillus YbgA halodurans] halodurans 302 303 YvbX 304Chitinase precursor [Streptomyces UniRef100_Q9L3E8 Streptomycesolivaceoviridis olivaceoviridis] 305 Extracellular metalloproteaseprecursor UniRef100_P39790 Bacillus subtilis Mpr [Bacillus subtilis] 306Glucose 1-dehydrogenase II [Bacillus UniRef100_P80869 Bacillus subtilisYcdF subtilis] 307 Hypothetical protein OB0244 UniRef100_Q8ETL6Oceanobacillus iheyensis [Oceanobacillus iheyensis] 308 Hypotheticalprotein ycdC [Bacillus UniRef100_O34772 Bacillus subtilis YcdC subtilis]309 310 Cell wall hydrolase cwlJ [Bacillus UniRef100_P42249 Bacillussubtilis CwlJ subtilis] 311 Hypothetical protein yceB [BacillusUniRef100_O34504 Bacillus subtilis YceB subtilis] 312 Hypotheticalprotein yvcE [Bacillus UniRef100_P40767 Bacillus subtilis YvcE subtilis]313 ABC transporter, permease [Bacillus UniRef100_Q637N2 Bacillus cereusYfiM cereus ZK] ZK 314 ABC transporter, ATP-binding proteinUniRef100_Q733P8 Bacillus cereus YfiL [Bacillus cereus] 315UPI00003CC482 UniRef100 entry UniRef100_UPI00003CC482 YsiA 316Hypothetical protein OB3113 UniRef100_Q8ELV2 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 317 BH3953 protein [Bacillus halodurans]UniRef100_Q9K5Y3 Bacillus halodurans 318 BH3951 protein [Bacillushalodurans] UniRef100_Q9K5Y4 Bacillus PadR halodurans 319 Stressresponse protein SCP2 [Bacillus UniRef100_P81100 Bacillus subtilis YceCsubtilis] 320 General stress protein 16U [Bacillus UniRef100_P80875Bacillus subtilis YceD subtilis] 321 Hypothetical protein yceE [BacillusUniRef100_O34384 Bacillus subtilis YceE subtilis] 322 Hypotheticalprotein yceF [Bacillus UniRef100_O34447 Bacillus subtilis YceF subtilis]323 Hypothetical protein [Bacillus cereus] UniRef100_Q72YD0 Bacilluscereus 324 YceG [Bacillus subtilis] UniRef100_O34809 Bacillus subtilisYceG 325 Hypothetical protein yceH [Bacillus UniRef100_O34833 Bacillussubtilis YceH subtilis] 326 UPI00003CB694 UniRef100 entryUniRef100_UPI00003CB694 CcdA 327 Hypothetical conserved proteinUniRef100_Q8ELB4 Oceanobacillus YdiL [Oceanobacillus iheyensis]iheyensis 328 Mta 329 Nitrate transporter [Bacillus subtilis]UniRef100_P42432 Bacillus subtilis NasA 330 L-lactate dehydrogenase[Bacillus UniRef100_P13714 Bacillus subtilis Ldh subtilis] 331 L-lactatepermease [Bacillus subtilis] UniRef100_P55910 Bacillus subtilis LctP 332YcgF protein [Bacillus subtilis] UniRef100_P94381 Bacillus subtilis YcgF333 Homologue of aromatic amino acids UniRef100_P94383 Bacillus subtilisYcgH transport protein of E. coli [Bacillus subtilis] 334NH(3)-dependent NAD(+) synthetase UniRef100_P08164 Bacillus subtilisNadE [Bacillus subtilis] 335 Shikimate kinase [Bacillus subtilis]UniRef100_P37944 Bacillus subtilis AroK 336 YcgL protein [Bacillussubtilis] UniRef100_P94389 Bacillus subtilis YcgL 337 Prolinedehydrogenase [Bacillus UniRef100_Q8RL79 Bacillus subtilis YcgMsubtilis] 338 1-pyrroline-5-carboxylate UniRef100_P94391 Bacillussubtilis YcgN dehydrogenase 2 [Bacillus subtilis] 339 Homologue ofproline permease of E. coli UniRef100_P94392 Bacillus subtilis YcgO[Bacillus subtilis] 340 Hypothetical protein ycgP [BacillusUniRef100_P94393 Bacillus subtilis YcgP subtilis] 341 YcgQ protein[Bacillus subtilis] UniRef100_P94394 Bacillus subtilis YcgQ 342 YcgR 343Cephalosporin-C deacetylase [Bacillus UniRef100_Q59233 Bacillus subtilisCah subtilis] 344 Transcriptional regulator [Bacillus UniRef100_Q9KF41Bacillus YdgG halodurans] halodurans 345 346 347 Probable amino-acid ABCtransporter UniRef100_P42399 Bacillus subtilis YckA permease proteinyckA [Bacillus subtilis] 348 Probable ABC transporter extracellularUniRef100_P42400 Bacillus subtilis YckB binding protein yckB precursor[Bacillus subtilis] 349 NAD(P)H dehydrogenase (Quinone);UniRef100_Q63FG6 Bacillus cereus YwrO possible modulator of drugactivity B ZK [Bacillus cereus ZK] 350 BH0315 protein [Bacillushalodurans] UniRef100_Q9KG01 Bacillus halodurans 351 Probablebeta-glucosidase [Bacillus UniRef100_P42403 Bacillus subtilis YckEsubtilis] 352 Nin [Bacillus amyloliquefaciens] UniRef100_Q70KK3 BacillusNin amyloliquefaciens 353 DNA-entry nuclease [Bacillus subtilis]UniRef100_P12667 Bacillus subtilis NucA 354 YbdM 355 Methyl-acceptingchemotaxis protein UniRef100_P39209 Bacillus subtilis TlpC tlpC[Bacillus subtilis] 356 Hypothetical protein yqcG [BacillusUniRef100_P45942 Bacillus subtilis YqcG subtilis] 357 Hydantoinutilization protein A UniRef100_Q7WCS3 Bordetella bronchiseptica[Bordetella bronchiseptica] 358 Hydantoin utilization protein BUniRef100_Q987J6 Rhizobium loti [Rhizobium loti] 359 UPI000027D233UniRef100 entry UniRef100_UPI000027D233 YvfK 360 358aa long hypotheticaltransporter UniRef100_Q9YB65 Aeropyrum MsmX ATP-binding protein[Aeropyrum pernix] pernix 361 AttA2-like ABC transporter, permeaseUniRef100_Q92ZH0 Rhizobium YesP protein [Rhizobium meliloti] meliloti362 YurM 363 SrfAA 364 SrfAB 365 SrfAC 366 SrfAD 367 YcxC 368 YcxD 3694′-phosphopantetheinyl transferase sfp UniRef100_P39135 Bacillussubtilis Sfp [Bacillus subtilis] 370 Predicted esterase of alpha/betaUniRef100_Q97HP7 Clostridium YbbA hydrolase superfamily, YBBA B.subtilis acetobutylicum ortholog [Clostridium acetobutylicum] 371Transcriptional regulator [Clostridium UniRef100_Q97LX8 Clostridium YdeEacetobutylicum] acetobutylicum 372 YczE [Bacillus subtilis]UniRef100_Q9F4F8 Bacillus subtilis YczE 373 Hypothetical protein[Symbiobacterium UniRef100_Q67MZ7 Symbiobacterium thermophilum]thermophilum 374 YfiL 375 376 Hypothetical protein [SymbiobacteriumUniRef100_Q67MZ9 Symbiobacterium thermophilum] thermophilum 377 378 379Methyl-accepting chemotaxis protein UniRef100_Q6HJV7 Bacillus TlpB[Bacillus thuringiensis] thuringiensis 380 YckI [Bacillus subtilis]UniRef100_Q9F4F9 Bacillus subtilis YckI 381 YckJ [Bacillus subtilis]UniRef100_Q9F4G0 Bacillus subtilis YckJ 382 YckK 383 RocR 384 Ornithineaminotransferase [Bacillus UniRef100_P38021 Bacillus subtilis RocDsubtilis] 385 Amino-acid permease rocE [Bacillus UniRef100_P39137Bacillus subtilis RocE subtilis] 386 Arginase [Bacillus subtilis]UniRef100_P39138 Bacillus subtilis RocF 387 Homologue of als operonregulatory UniRef100_P94403 Bacillus subtilis YclA protein AlsR of B.subtilis [Bacillus subtilis] 388 Probable aromatic acid decarboxylaseUniRef100_P94404 Bacillus subtilis YclB [Bacillus subtilis] 389Hypothetical protein yclC [Bacillus UniRef100_P94405 Bacillus subtilisYclC subtilis] 390 YclD protein [Bacillus subtilis] UniRef100_P94406Bacillus subtilis YclD 391 392 393 394 Hypothetical protein [BacillusUniRef100_Q6HMQ9 Bacillus thuringiensis thuringiensis] 395 Hypotheticalprotein OB2810 UniRef100_Q8EMN2 Oceanobacillus iheyensis [Oceanobacillusiheyensis] 396 397 YxiD 398 399 YxiB 400 Sugar ABC transporter [BacillusUniRef100_Q9K7B8 Bacillus RbsB halodurans] halodurans 401 Two-componentsensor histidine kinase UniRef100_Q9K7B9 Bacillus YesM [Bacillushalodurans] halodurans 402 YesN 403 Multiple sugar transport systemUniRef100_Q9K7C2 Bacillus RbsB [Bacillus halodurans] halodurans 404L-arabinose transport ATP-binding UniRef100_Q9S472 Bacillus RbsA proteinaraG [Bacillus stearothermophilus stearothermophilus] 405 L-arabinosemembrane permease UniRef100_Q9S471 Bacillus RbsC [Bacillusstearothermophilus] stearothermophilus 406 Transporter [Bacillushalodurans] UniRef100_Q9K9F3 Bacillus YbfB halodurans 407 YbfA protein[Bacillus subtilis] UniRef100_O31443 Bacillus subtilis YbfA 408Beta-D-galactosidase [Bacillus UniRef100_Q45093 Bacillus circulans LacAcirculans] 409 Phy 410 Hypothetical transporter yclF [BacillusUniRef100_P94408 Bacillus subtilis YclF subtilis] 411 YclG protein[Bacillus subtilis] UniRef100_P94409 Bacillus subtilis YclG 412 413GerKA 414 Spore germination protein KC precursor UniRef100_P49941Bacillus subtilis GerKC [Bacillus subtilis] 415 Spore germinationprotein KB [Bacillus UniRef100_P49940 Bacillus subtilis GerKB subtilis]416 Mta 417 Hypothetical protein yclH [Bacillus UniRef100_P94411Bacillus subtilis YclH subtilis] 418 Hypothetical protein yclI [BacillusUniRef100_P94412 Bacillus subtilis YclI subtilis] 419 Hypotheticalsensory transduction UniRef100_P94413 Bacillus subtilis YclJ proteinyclJ [Bacillus subtilis] 420 Hypothetical sensor-like histidineUniRef100_P94414 Bacillus subtilis YclK kinase yclK [Bacillus subtilis]421 Methyl-accepting chemotaxis protein UniRef100_Q9K632 Bacillus TlpA[Bacillus halodurans] halodurans 422 Probable aspartokinase [BacillusUniRef100_P94417 Bacillus subtilis YclM subtilis] 423 Homologue offerric anguibactin UniRef100_P94418 Bacillus subtilis YclN transportsystem permerase protein FatD of V. anguillarum [Bacillus subtilis] 424Homologue of ferric anguibactin UniRef100_P94419 Bacillus subtilis YclOtransport system permerase protein FatC of V. anguillarum [Bacillussubtilis] 425 Homologue of iron dicitrate transport UniRef100_P94420Bacillus subtilis YclP ATP-binding protein FecE of E. coli [Bacillussubtilis] 426 Ferric anguibactin-binding protein UniRef100_P94421Bacillus subtilis YclQ precusor FatB of V. anguillarum [Bacillussubtilis] 427 Homologue of multidrug resistance UniRef100_P94422Bacillus subtilis YcnB protein B, EmrB, of E. coli [Bacillus subtilis]428 YcnC protein [Bacillus subtilis] UniRef100_P94423 Bacillus subtilisYcnC 429 Hypothetical protein [Bacillus cereus UniRef100_Q636R0 Bacilluscereus YqiQ ZK] ZK 430 Hypothetical oxidoreductase ycnD UniRef100_P94424Bacillus subtilis YcnD [Bacillus subtilis] 431 Hypothetical protein ycnE[Bacillus UniRef100_P94425 Bacillus subtilis subtilis] 432 YczG protein[Bacillus subtilis] UniRef100_O31480 Bacillus subtilis 433 Homologue ofregulatory protein MocR UniRef100_P94426 Bacillus subtilis GabR of R.meliloti [Bacillus subtilis] 434 Probable 4-aminobutyrateUniRef100_P94427 Bacillus subtilis GabT aminotransferase (EC 2.6.1.19)((S)-3- amino-2-methylpropionate transaminase) [Bacillus subtilis] 435Cationic amino acid transporter UniRef100_Q8ESX7 Oceanobacillus YhdG[Oceanobacillus iheyensis] iheyensis 436 Homologue of succinatesemialdehyde UniRef100_P94428 Bacillus subtilis GabD dehydrogenase GabDof E. coli [Bacillus subtilis] 437 YwfM 438 439 YcnI protein [Bacillussubtilis] UniRef100_P94431 Bacillus subtilis YcnI 440 Homologue ofcopper export protein UniRef100_P94432 Bacillus subtilis YcnJ PcoD of E.coli [Bacillus subtilis] 441 YcnK protein [Bacillus subtilis]UniRef100_P94433 Bacillus subtilis YcnK 442 Assimilatory nitratereductase electron UniRef100_P42433 Bacillus subtilis NasB transfersubunit [Bacillus subtilis] 443 Assimilatory nitrate reductase catalyticUniRef100_P42434 Bacillus subtilis NasC subunit [Bacillus subtilis] 444Nitrite reductase [NAD(P)H] [Bacillus UniRef100_P42435 NAD(P)H NasDsubtilis] 445 Assimilatory nitrite reductase UniRef100_P42436 NAD(P)HNasE [NAD(P)H] small subunit [Bacillus subtilis] 446 Uroporphyrin-IIIC-methyltransferase UniRef100_P42437 Bacillus subtilis NasF [Bacillussubtilis] 447 Hypothetical transcriptional regulator UniRef100_O05494Bacillus subtilis YdhC ydhC [Bacillus subtilis] 448 Sodium-dependenttransporter UniRef100_Q8ENE3 Oceanobacillus YflS [Oceanobacillusiheyensis] iheyensis 449 Hypothetical protein ycsD [BacillusUniRef100_P42961 Bacillus subtilis subtilis] 450 UPI00003CC424 UniRef100entry UniRef100_UPI00003CC424 451 Possible transcriptionalantiterminator, UniRef100_Q63A16 Bacillus cereus MtlR bglG family[Bacillus cereus ZK] ZK 452 Putative sugar-specific PTS componentUniRef100_Q7X1N9 Lactococcus raffinolactis EIIB [Lactococcusraffinolactis] 453 SgaT protein [Mannheimia UniRef100_Q65WA2 Mannheimiasucciniciproducens MBEL55E] succiniciproducens MBEL55E 454Transketolase, N-terminal subunit UniRef100_Q8E202 Streptococcus Tkt[Streptococcus agalactiae] agalactiae 455 Putative transketolase[Salmonella UniRef100_Q8ZND5 Salmonella Dxs typhimurium] typhimurium 456Hypothetical protein ycsE [Bacillus UniRef100_P42962 Bacillus subtilisYcsE subtilis] 457 Hypothetical UPF0271 protein ycsF UniRef100_P42963Bacillus subtilis YcsF [Bacillus subtilis] 458 Hypothetical protein ycsG[Bacillus UniRef100_P42964 Bacillus subtilis YcsG subtilis] 459Hypothetical UPF0317 protein ycsI UniRef100_P42966 Bacillus subtilisYcsI [Bacillus subtilis] 460 Kinase A inhibitor [Bacillus subtilis]UniRef100_P60495 Bacillus subtilis KipI 461 KipI antagonist [Bacillussubtilis] UniRef100_Q7WY77 Bacillus subtilis KipA 462 HTH-typetranscriptional regulator kipR UniRef100_P42968 Bacillus subtilis KipR[Bacillus subtilis] 463 Hypothetical protein ycsK [BacillusUniRef100_P42969 Bacillus subtilis YcsK subtilis] 464 PTS system,mannitol-specific IIABC UniRef100_P42956 Bacillus subtilis MtlAcomponent [Bacillus subtilis] 465 Mannitol-1-phosphate 5-UniRef100_P42957 Bacillus subtilis MtlD dehydrogenase [Bacillussubtilis] 466 YdaA protein [Bacillus subtilis] UniRef100_P96574 Bacillussubtilis MtlR 467 General stress protein 39 [Bacillus UniRef100_P80873Bacillus subtilis YdaD subtilis] 468 Hypothetical protein ydaE [BacillusUniRef100_P96578 Bacillus subtilis YdaE subtilis] 469 Hypotheticalprotein [Bacillus anthracis] UniRef100_Q81U55 Bacillus anthracis 470General stress protein 26 [Bacillus UniRef100_P80238 Bacillus subtilisYdaG subtilis] 471 YdaH protein [Bacillus subtilis] UniRef100_P96581Bacillus subtilis YdaH 472 Lin0463 protein [Listeria innocua]UniRef100_Q92EJ6 Listeria innocua YvhJ 473 YdzA protein [Bacillussubtilis] UniRef100_O31485 Bacillus subtilis 474 BH0424 protein[Bacillus halodurans] UniRef100_Q9KFQ4 Bacillus halodurans 475 HTH-typetranscriptional regulator lrpC UniRef100_P96582 Bacillus subtilis LrpC[Bacillus subtilis] 476 PROBABLE DNA TOPOISOMERASE UniRef100_P96583Bacillus subtilis TopB III [Bacillus subtilis] 477 478 479 480 YdaO 481YdaO 482 YdaP protein [Bacillus subtilis] UniRef100_P96591 Bacillussubtilis YdaP 483 484 UPI00003CC069 UniRef100 entryUniRef100_UPI00003CC069 485 IS1627s1-related, transposase [BacillusUniRef100_Q7CMD0 Bacillus anthracis str. A2012 anthracis str. A2012] 486487 Similar to ribosomal-protein-serine N- UniRef100_Q99WN5Staphylococcus YdaF acetyltransferase [Staphylococcus aureus aureus] 488Manganese transport protein mntH UniRef100_P96593 Bacillus subtilis MntH[Bacillus subtilis] 489 490 AnsB 491 YojK 492 YdaT protein [Bacillussubtilis] UniRef100_P96595 Bacillus subtilis YdaT 493 Hypotheticalprotein ydbA [Bacillus UniRef100_P96596 Bacillus subtilis YdbA subtilis]494 Na+/H+ antiporter NhaC [Bacillus UniRef100_Q81FX8 Bacillus cereusNhaC cereus] 495 YdbB protein [Bacillus subtilis] UniRef100_P96597Bacillus subtilis YdbB 496 Glucose starvation-inducible protein BUniRef100_P26907 Bacillus subtilis GsiB [Bacillus subtilis] 497Hypothetical UPF0118 protein ydbI UniRef100_P96604 Bacillus subtilisYdbI [Bacillus subtilis] 498 GltT 499 YdbJ protein [Bacillus subtilis]UniRef100_P96605 Bacillus subtilis YdbJ 500 YdbK protein [Bacillussubtilis] UniRef100_P96606 Bacillus subtilis YdbK 501 Hypotheticalprotein ydbL [Bacillus UniRef100_P96607 Bacillus subtilis YdbL subtilis]502 YdbM protein [Bacillus subtilis] UniRef100_P96608 Bacillus subtilisYdbM 503 504 505 YdbP protein [Bacillus subtilis] UniRef100_P96611Bacillus subtilis YdbP 506 D-alanine--D-alanine ligase [BacillusUniRef100_P96612 Bacillus subtilis Ddl subtilis] 507 MurF 508 Esterase[Oceanobacillus iheyensis] UniRef100_Q8ESM0 Oceanobacillus YvaKiheyensis 509 YdbR protein [Bacillus subtilis] UniRef100_P96614 Bacillussubtilis YdbR 510 YdbS protein [Bacillus subtilis] UniRef100_P96615Bacillus subtilis YdbS 511 YdbT protein [Bacillus subtilis]UniRef100_P96616 Bacillus subtilis YdbT 512 YdcA protein [Bacillussubtilis] UniRef100_P96617 Bacillus subtilis YdcA 513 YdcC 514 Alr 515516 YdcE protein [Bacillus subtilis] UniRef100_P96622 Bacillus subtilisYdcE 517 RsbR 518 RsbS 519 RsbT 520 RsbU 521 RsbV 522 RsbW 523 SigB 524RsbX 525 YdcI protein [Bacillus subtilis] UniRef100_O31489 Bacillussubtilis YdcI 526 Transcriptional regulator, TetR familyUniRef100_Q6HGY5 Bacillus YxbF [Bacillus thuringiensis] thuringiensis527 Lin1189 protein [Listeria innocua] UniRef100_Q92CI2 Listeria innocuaYdgH 528 529 Protein sprT-like [Bacillus subtilis] UniRef100_P96628Bacillus subtilis YdcK 530 Possible transporter, EamA familyUniRef100_Q638K5 Bacillus cereus ZK [Bacillus cereus ZK] 531 532Hypothetical protein ORF00034 UniRef100_O87235 Lactococcus lactis[Lactococcus lactis] 533 Delta5 acyl-lipid desaturase [BacillusUniRef100_Q81C02 Bacillus cereus Des cereus] 534 Cold shock protein cspC[Bacillus UniRef100_P39158 Bacillus subtilis subtilis] 535 YogA 536Membrane protein, putative [Bacillus UniRef100_Q734Y0 Bacillus cereusYyaS cereus] 537 Transcriptional regulator, MarR family UniRef100_Q734X9Bacillus cereus YybA [Bacillus cereus] 538 Acetyltransferase, GNATfamily UniRef100_Q734X8 Bacillus cereus PaiA [Bacillus cereus] 539Protease synthase and sporulation UniRef100_Q734X7 Bacillus cereus PaiBnegative regulatory protein PAI 2 [Bacillus cereus] 540 BH0654 protein[Bacillus halodurans] UniRef100_Q9KF33 Bacillus RocF halodurans 541 542YdeO protein [Bacillus subtilis] UniRef100_P96672 Bacillus subtilis YdeO543 Transporter, LysE family [Bacillus UniRef100_Q81DI7 Bacillus cereusYrhP cereus] 544 YwqM 545 546 Permease, putative [Bacillus anthracis]UniRef100_Q81QY7 Bacillus YvqJ anthracis 547 Putative cyclase[Rhodopseudomonas UniRef100_Q6N497 Rhodopseudomonas palustris palustris]548 Hypothetical transport protein ydgF UniRef100_P96704 Bacillussubtilis YdgF [Bacillus subtilis] 549 550 YknW 551 RNA polymerase sigmafactor sigV UniRef100_O05404 Bacillus subtilis SigV [Bacillus subtilis]552 Putative anti-SigV factor [Bacillus UniRef100_O05403 Bacillussubtilis YrhM subtilis] 553 Hypothetical protein yrhL [BacillusUniRef100_O05402 Bacillus subtilis YrhL subtilis] 554 YdgK 555 YwpD 556LytT 557 Collagen adhesion protein [Bacillus UniRef100_Q630P2 Bacilluscereus ZK cereus ZK] 558 Lin0929 protein [Listeria innocua]UniRef100_Q92D88 Listeria innocua 559 560 Metabolite transport protein[Bacillus UniRef100_O34718 Bacillus subtilis YdjK subtilis] 561Thiamine-monophosphate kinase UniRef100_O05514 Bacillus subtilis ThiL[Bacillus subtilis] 562 Hypothetical UPF0079 protein ydiBUniRef100_O05515 Bacillus subtilis YdiB [Bacillus subtilis] 563 YdiCprotein [Bacillus subtilis] UniRef100_O05516 Bacillus subtilis YdiC 564YdiD protein [Bacillus subtilis] UniRef100_O05517 Bacillus subtilis YdiD565 Probable O-sialoglycoprotein UniRef100_O05518 Bacillus subtilis Gcpendopeptidase [Bacillus subtilis] 566 YdiF 567 YdiF 568 Molybdenumcofactor biosynthesis UniRef100_O05520 Bacillus subtilis YdiG protein C[Bacillus subtilis] 569 Redox-sensing transcriptional repressorUniRef100_O05521 Bacillus subtilis YdiH rex [Bacillus subtilis] 570 YdiI[Bacillus halodurans] UniRef100_Q9Z9P5 Bacillus halodurans 571 TatCY 572Hypothetical lipoprotein ydiK precursor UniRef100_O05524 Bacillussubtilis [Bacillus subtilis] 573 YdiL 574 10 kDa chaperonin [Bacillussubtilis] UniRef100_P28599 Bacillus subtilis 575 60 kDa chaperonin[Bacillus subtilis] UniRef100_P28598 Bacillus subtilis GroEL 576 577 578Hypothetical protein yolD UniRef100_O64030 Bacteriophage SPBc2[Bacteriophage SPBc2] 579 580 581 YoaR [Bacillus subtilis]UniRef100_O34611 Bacillus subtilis YoaR 582 Hypothetical protein yfmQ[Bacillus UniRef100_O06475 Bacillus subtilis YfmQ subtilis] 583 584 YoqWprotein [Bacteriophage SPBc2] UniRef100_O64131 Bacteriophage YoqW SPBc2585 Lin2076 protein [Listeria innocua] UniRef100_Q92A46 Listeria innocuaYerO 586 PEP synthase [Bacillus subtilis] UniRef100_O34309 Bacillussubtilis Pps 587 Hypothetical protein yoaF [Bacillus UniRef100_O31829Bacillus subtilis subtilis] 588 Hypothetical protein [StaphylococcusUniRef100_Q6GK76 Staphylococcus aureus aureus] 589 Short-chaindehydrodenase UniRef100_Q97LM1 Clostridium DltE [Clostridiumacetobutylicum] acetobutylicum 590 Type B carboxylesterase [Bacillus sp.UniRef100_Q9L378 Bacillus sp. BP-7 PnbA BP-7] 591 Inositol transportprotein UniRef100_Q8ESX2 Oceanobacillus IolF [Oceanobacillus iheyensis]iheyensis 592 Phage shock protein A homolog UniRef100_P54617 Bacillussubtilis PspA [Bacillus subtilis] 593 YdjG protein [Bacillus subtilis]UniRef100_O34434 Bacillus subtilis YdjG 594 YdjH protein [Bacillussubtilis] UniRef100_O35004 Bacillus subtilis YdjH 595 YdjI protein[Bacillus subtilis] UniRef100_O34789 Bacillus subtilis YdjI 596 Putativeoxidoreductase UniRef100_Q67S08 Symbiobacterium YtmO [Symbiobacteriumthermophilum] thermophilum 597 YrhO [Bacillus subtilis] UniRef100_O05405Bacillus subtilis YrhO 598 YrhP 599 Helix-turn-helix domain protein[Bacillus UniRef100_Q73C00 Bacillus cereus cereus] 600 Stage Vsporulation protein E [Bacillus UniRef100_Q9K7T4 Bacillus SpoVEhalodurans] halodurans 601 Stage V sporulation protein E [BacillusUniRef100_Q9K7T3 Bacillus FtsW halodurans] halodurans 602 603Hypothetical protein [Bacillus cereus] UniRef100_Q72Z89 Bacillus cereus604 BH1889 protein [Bacillus halodurans] UniRef100_Q9KBN6 Bacillus YobVhalodurans 605 YjeA 606 YjeA 607 TreA 608 Putative HTH-typetranscriptional UniRef100_O06987 Bacillus subtilis YvdE regulator yvdE[Bacillus subtilis] 609 Hypothetical protein yvdF [BacillusUniRef100_O06988 Bacillus subtilis YvdF subtilis] 610 Hypotheticalprotein yvdG [Bacillus UniRef100_O06989 Bacillus subtilis YvdG subtilis]611 Hypothetical protein yvdH [Bacillus UniRef100_O06990 Bacillussubtilis YvdH subtilis] 612 Hypothetical protein yvdI [BacillusUniRef100_O06991 Bacillus subtilis YvdI subtilis] 613 Hypotheticalprotein yvdJ [Bacillus UniRef100_O06992 Bacillus subtilis YvdJ subtilis]614 Hypothetical glycosyl hydrolase yvdK UniRef100_O06993 Bacillussubtilis YvdK [Bacillus subtilis] 615 Oligo-1,6-glucosidase [Bacillussubtilis] UniRef100_O06994 Bacillus subtilis MalL 616 Putativebeta-phosphoglucomutase UniRef100_O06995 Bacillus subtilis PgcM[Bacillus subtilis] 617 Tyrosyl-tRNA synthetase 2 [BacillusUniRef100_P25151 Bacillus subtilis TyrZ subtilis] 618 Putative HTH-typetranscriptional UniRef100_P25150 Bacillus subtilis YwaE regulator ywaE[Bacillus subtilis] 619 620 Hypothetical protein SE2399 UniRef100_Q8CQM7Staphylococcus epidermidis [Staphylococcus epidermidis 621 Hypotheticalprotein ydjM [Bacillus UniRef100_P40775 Bacillus subtilis YdjM subtilis]622 YdjN protein [Bacillus subtilis] UniRef100_O34353 Bacillus subtilisYdjN 623 Hypothetical protein [Bacillus cereus] UniRef100_Q81AT6Bacillus cereus YeaA 624 625 Signal peptidase I [Bacillus cereus]UniRef100_Q73C25 Bacillus cereus SipS 626 627 Hypothetical protein yhfK[Bacillus UniRef100_O07609 Bacillus subtilis YhfK subtilis] 628Transcriptional regulator [Bacillus UniRef100_Q9K766 Bacillus YdeEhalodurans] halodurans 629 Spore coat protein A [Bacillus subtilis]UniRef100_P07788 Bacillus subtilis CotA 630 YkrP 631 Extracellularprotein [Lactobacillus UniRef100_Q88T27 Lactobacillus YcdA plantarumplantarum 632 Hypothetical UPF0018 protein yeaB UniRef100_P46348Bacillus subtilis YeaB [Bacillus subtilis] 633 YeaC [Bacillus subtilis]UniRef100_P94474 Bacillus subtilis YeaC 634 Hypothetical protein[Bacillus cereus] UniRef100_Q739D8 Bacillus cereus YeaD 635 YebA[Bacillus subtilis] UniRef100_P94476 Bacillus subtilis YebA 636 GMPsynthase [glutamine-hydrolyzing] UniRef100_P29727 glutamine- GuaA[Bacillus subtilis] hydrolyzing 637 Hypoxanthine/guanine permeaseUniRef100_O34987 Bacillus subtilis PbuG [Bacillus subtilis] 638 YebC 639Hypothetical UPF0316 protein yebE UniRef100_O34624 Bacillus subtilisYebE [Bacillus subtilis] 640 YebG protein [Bacillus subtilis]UniRef100_O34700 Bacillus subtilis 641 PhosphoribosylaminoimidazoleUniRef100_P12044 Bacillus subtilis PurE carboxylase catalytic subunit[Bacillus subtilis] 642 PurK 643 Adenylosuccinate lyase [BacillusUniRef100_P12047 Bacillus subtilis PurB subtilis] 644Phosphoribosylaminoimidazole- UniRef100_P12046 Bacillus subtilis PurCsuccinocarboxamide synthase [Bacillus subtilis] 645 Hypothetical UPF0062protein yexA UniRef100_P12049 Bacillus subtilis [Bacillus subtilis] 646Phosphoribosylformylglycinamidine UniRef100_P12041 Bacillus subtilisPurQ synthase I [Bacillus subtilis] 647Phosphoribosylformylglycinamidine UniRef100_P12042 Bacillus subtilisPurL synthase II [Bacillus subtilis] 648 AmidophosphoribosyltransferaseUniRef100_P00497 Bacillus subtilis PurF precursor [Bacillus subtilis]649 PurM 650 Phosphoribosylglycinamide UniRef100_P12040 Bacillussubtilis PurN formyltransferase [Bacillus subtilis] 651 Bifunctionalpurine biosynthesis protein UniRef100_P12048 Includes: PurH purH[Includes: PhosphoribosylaminoimidazolecarboxamidePhosphoribosylaminoimidazolecarboxamide formyltransferaseformyltransferase (EC 2.1.2.3) (EC 2.1.2.3) (AICAR transformylase); IMP(AICAR cyclohydrolase (EC 3.5.4.10) transformylase); (Inosinicase) (IMPsynthetase) (ATIC)] IMP [Bacillus subtilis] cyclohydrolase (EC 3.5.4.10)(Inosinicase) (IMP synthetase) (ATIC) 652 Phosphoribosylamine--glycineligase UniRef100_P12039 Bacillus subtilis PurD [Bacillus subtilis] 653YxbF 654 Putative cytochrome P450 yjiB [Bacillus UniRef100_O34374Bacillus subtilis YjiB subtilis] 655 656 Hypothetical lipoprotein yybPprecursor UniRef100_P37488 Bacillus subtilis YybP [Bacillus subtilis]657 Transposase [Thermoanaerobacter UniRef100_Q8RCM3 Thermoanaerobactertengcongensis] tengcongensis 658 Hypothetical protein [Bacillusanthracis] UniRef100_Q81ZG4 Bacillus anthracis 659 Putative adeninedeaminase yerA UniRef100_O34909 Bacillus subtilis YerA [Bacillussubtilis] 660 YerB protein [Bacillus subtilis] UniRef100_O34968 Bacillussubtilis YerB 661 YecD [Bacillus subtilis] UniRef100_Q7BVT7 Bacillussubtilis YerC 662 PcrB protein homolog [Bacillus subtilis]UniRef100_O34790 Bacillus subtilis PcrB 663 ATP-dependent DNA helicasepcrA UniRef100_O34580 Bacillus subtilis PcrA [Bacillus subtilis] 664 DNAligase [Bacillus subtilis] UniRef100_O31498 Bacillus subtilis LigA 665YerH protein [Bacillus subtilis] UniRef100_O34629 Bacillus subtilis YerH666 BH0586 protein [Bacillus halodurans] UniRef100_Q9KF99 Bacillushalodurans 667 668 669 BH0589 protein [Bacillus halodurans]UniRef100_Q9KF96 Bacillus halodurans 670 Phosphotriesterase homologyprotein UniRef100_P45548 Escherichia coli [Escherichia coli] 671 Similarto unknown protein YhfS of UniRef100_Q7N5F2 Photorhabdus Csd Escherichiacoli [Photorhabdus luminescens luminescens] 672 PhosphopentomutaseUniRef100_Q67S06 Symbiobacterium Drm [Symbiobacterium thermophilum]thermophilum 673 Putative alanine racemase UniRef100_Q67S05Symbiobacterium [Symbiobacterium thermophilum] thermophilum 674Glucosamine-6-phosphate deaminase UniRef100_Q8ESL6 Oceanobacillus NagB[Oceanobacillus iheyensis] iheyensis 675 SapB protein [Bacillussubtilis] UniRef100_Q45514 Bacillus subtilis SapB 676 OpuE 677Glutamyl-tRNA(Gln) amidotransferase UniRef100_O06492 Bacillus subtilissubunit C [Bacillus subtilis] 678 Glutamyl-tRNA(Gln) amidotransferaseUniRef100_O06491 Bacillus subtilis GatA subunit A [Bacillus subtilis]679 GatB 680 Hypothetical protein [Bacillus UniRef100_Q848Y2 Bacillusmegaterium megaterium] 681 682 YdhT 683 684 Putative HTH-typetranscriptional UniRef100_O31500 Bacillus subtilis YerO regulator yerO[Bacillus subtilis] 685 Swarming motility protein swrC [BacillusUniRef100_O31501 Bacillus subtilis YerP subtilis] 686 YjcK 687Inosine-uridine preferring nucleoside UniRef100_Q81DM6 Bacillus cereushydrolase [Bacillus cereus] 688 YerQ protein [Bacillus subtilis]UniRef100_O31502 Bacillus subtilis YerQ 689 Hypothetical RNAmethyltransferase UniRef100_O31503 Bacillus subtilis YefA yefA [Bacillussubtilis] 690 Type I restriction-modification system UniRef100_Q817S1Bacillus cereus specificity subunit [Bacillus cereus] 691 Type Irestriction-modification system UniRef100_Q817S2 Bacillus cereusmethylation subunit [Bacillus cereus] 692 Type IC specificity subunitUniRef100_Q9RNW0 Streptococcus thermophilus [Streptococcus thermophilus]693 Type I restriction-modification system UniRef100_Q817S4 Bacilluscereus restriction subunit [Bacillus cereus] 694 695 Beta-glucosidesPTS, EIIBCA UniRef100_Q88T54 Lactobacillus BglP [Lactobacillusplantarum] plantarum 696 6-phospho-beta-glucosidase UniRef100_Q88T55Lactobacillus BglH [Lactobacillus plantarum] plantarum 697 LicT 698Response regulator aspartate UniRef100_O34930 Bacillus subtilis RapKphosphatase K [Bacillus subtilis] 699 700 Methyltransferase [Bacilluscereus ZK] UniRef100_Q639N2 Bacillus cereus ZK 701 Hypothetical UPF0082protein UniRef100_P62032 Bacillus cereus YeeI BCE0595 [Bacillus cereus]702 Putative HTH-type transcriptional UniRef100_O28646 Archaeoglobusfulgidus regulator AF1627 [Archaeoglobus fulgidus] 703 704 YfmT[Bacillus subtilis] UniRef100_O06478 Bacillus subtilis YfmT 705 YfmS[Bacillus subtilis] UniRef100_O06477 Bacillus subtilis YfmS 706 YflS 707YfmR [Bacillus subtilis] UniRef100_O06476 Bacillus subtilis YfmR 708 709YciA protein [Bacillus subtilis] UniRef100_P94398 Bacillus subtilis YciA710 UPI00002BDF65 UniRef100 entry UniRef100_UPI00002BDF65 YpdA 711Ferrus ion transporter protein UniRef100_Q6U5S9 Klebsiella pneumoniae[Klebsiella pneumoniae] 712 713 YciC protein [Bacillus UniRef100_Q70KK8Bacillus YciC amyloliquefaciens] amyloliquefaciens 714 BioW 715Adenosylmethionine-8-amino-7- UniRef100_P53555 Bacillus subtilis BioAoxononanoate aminotransferase [Bacillus subtilis] 7168-amino-7-oxononanoate synthase UniRef100_P53556 Bacillus subtilis BioF[Bacillus subtilis] 717 BioD protein [Bacillus UniRef100_Q70JZ0 BacillusBioD amyloliquefaciens] amyloliquefaciens 718 BioB protein [BacillusUniRef100_Q70JZ1 Bacillus BioB amyloliquefaciens] amyloliquefaciens 719BioI protein [Bacillus amyloliquefaciens] UniRef100_Q70JZ2 Bacillus BioIamyloliquefaciens 720 BH1501 protein [Bacillus halodurans]UniRef100_Q9KCR8 Bacillus halodurans 721 YfmP 722 Multidrug effluxprotein yfmO [Bacillus UniRef100_O06473 Bacillus subtilis YfmO subtilis]723 YfmM protein [Bacillus subtilis] UniRef100_O34512 Bacillus subtilisYfmM 724 YfmL protein [Bacillus subtilis] UniRef100_O34750 Bacillussubtilis YfmL 725 YfmJ protein [Bacillus subtilis] UniRef100_O34812Bacillus subtilis YfmJ 726 Hypothetical protein yfmB [BacillusUniRef100_O34626 Bacillus subtilis YfmB subtilis] 727 General stressprotein 17M [Bacillus UniRef100_P80241 Bacillus subtilis YflT subtilis]728 YflS 729 YflS 730 Putative permease [Clostridium tetani]UniRef100_Q895A0 Clostridium tetani 731 Possible Zn-dependent hydrolase,UniRef100_Q6HKH5 Bacillus YqgX beta-lactamase superfamily [Bacillusthuringiensis thuringiensis] 732 YflN protein [Bacillus subtilis]UniRef100_O34409 Bacillus subtilis YflN 733 Zink-carboxypeptidase[Clostridium UniRef100_Q898E1 Clostridium tetani tetani] 734 Nitricoxide synthase oxygenase UniRef100_O34453 Bacillus subtilis YflM[Bacillus subtilis] 735 Membrane protein, putative [BacillusUniRef100_Q72YN4 Bacillus cereus YvaZ cereus] 736 Transcriptionalregulator, ArsR family UniRef100_Q632K6 Bacillus cereus ZK [Bacilluscereus ZK] 737 Putative acylphosphatase [Bacillus UniRef100_O35031Bacillus subtilis subtilis] 738 YflK protein [Bacillus subtilis]UniRef100_O34542 Bacillus subtilis YflK 739 740 741 YflG protein[Bacillus subtilis] UniRef100_O34484 Bacillus subtilis YflG 742 YflE 743Hypothetical protein yflB [Bacillus UniRef100_O34887 Bacillus subtilissubtilis] 744 YflA 745 Probable PTS system, trehalose- UniRef100_P39794Bacillus subtilis TreP specific IIBC component [Bacillus subtilis] 746Alpha-glucosidase [Bacillus sp. UniRef100_Q9L872 Bacillus sp. TreADG0303] DG0303 747 Trehalose operon transcriptional UniRef100_P39796Bacillus subtilis TreR repressor [Bacillus subtilis] 748Acetyltransferases UniRef100_Q8RBZ9 Thermoanaerobacter YvfD[Thermoanaerobacter tengcongensis] tengcongensis 749 750 751 Predictedpyridoxal phosphate- UniRef100_Q8RBY7 Thermoanaerobacter SpsC dependentenzyme apparently involved tengcongensis in regulation of cell wallbiogenesis [Thermoanaerobacter tengcongensis] 752 YkuQ 753 UPI000029FB28UniRef100 entry UniRef100_UPI000029FB28 YtcB 754 Hypothetical proteinMA2181 UniRef100_Q8TNU7 Methanosarcina acetivorans [Methanosarcinaacetivorans] 755 756 Beta 1,4 glucosyltransferase [BacillusUniRef100_Q81GJ1 Bacillus cereus YolJ cereus] 757 Predicted pyridoxalphosphate- UniRef100_Q8RC02 Thermoanaerobacter SpsC dependent enzymeapparently involved tengcongensis in regulation of cell wall biogenesis[Thermoanaerobacter tengcongensis] 758 Predicted dehydrogenases andrelated UniRef100_Q8RC00 Thermoanaerobacter YrbE proteins[Thermoanaerobacter tengcongensis tengcongensis] 759UDP-glucose:GDP-mannose UniRef100_Q8CXB6 Oceanobacillus TuaDdehydrogenase [Oceanobacillus iheyensis iheyensis] 760 Hypotheticalprotein [Bacillus cereus UniRef100_Q635H5 Bacillus cereus YqkD ZK] ZK761 Hypothetical UPF0087 protein ydeP UniRef100_P96673 Bacillus subtilisYdeP [Bacillus subtilis] 762 Putative NAD(P)H nitroreductase yfkOUniRef100_O34475 Bacillus subtilis YfkO [Bacillus subtilis] 763 YfkN 764General stress protein 18 [Bacillus UniRef100_P80876 Bacillus subtilisYfkM subtilis] 765 YfkK protein [Bacillus subtilis] UniRef100_O35019Bacillus subtilis 766 Amino acid transporter [Bacillus UniRef100_Q9K5Q5Bacillus YflA halodurans] halodurans 767 YfkJ protein [Bacillussubtilis] UniRef100_O35016 Bacillus subtilis YfkJ 768 Hypotheticalprotein yfkI precursor UniRef100_O34418 Bacillus subtilis YfkI [Bacillussubtilis] 769 YfkH protein [Bacillus subtilis] UniRef100_O34437 Bacillussubtilis YfkH 770 YfkF protein [Bacillus subtilis] UniRef100_O34929Bacillus subtilis YfkF 771 Hypothetical conserved proteinUniRef100_Q8ELS1 Oceanobacillus iheyensis [Oceanobacillus iheyensis] 772YfkE protein [Bacillus subtilis] UniRef100_O34840 Bacillus subtilis YfkE773 YfkD protein [Bacillus subtilis] UniRef100_O34579 Bacillus subtilisYfkD 774 Thioredoxin-like oxidoreductases UniRef100_Q81IC7 Bacilluscereus YfkA [Bacillus cereus] 775 YljT protein [Bacillus subtilis]UniRef100_O35041 Bacillus subtilis 776 YfjS 777 UPI000029390C UniRef100entry UniRef100_UPI000029390C AraM 778 779 YfjQ protein [Bacillussubtilis] UniRef100_O31543 Bacillus subtilis YfjQ 780 YfjP protein[Bacillus subtilis] UniRef100_O31544 Bacillus subtilis YfjP 781 YfjO 782YfjM protein [Bacillus subtilis] UniRef100_O31547 Bacillus subtilis YfjM783 784 Hypothetical protein yfjL [Bacillus UniRef100_P40773 Bacillussubtilis YfjL subtilis] 785 UPI00003CB259 UniRef100 entryUniRef100_UPI00003CB259 YvkB 786 YdhE protein [Bacillus subtilis]UniRef100_O05496 Bacillus subtilis YdhE 787 Hypothetical protein yckDprecursor UniRef100_P42402 Bacillus subtilis [Bacillus subtilis] 788Hypothetical metabolite transport UniRef100_O34691 Bacillus subtilisYceI protein yceI [Bacillus subtilis] 789 SacX 790 Levansucrase andsucrase synthesis UniRef100_P15401 Bacillus subtilis SacY operonantiterminator [Bacillus subtilis] 791 Hypothetical protein [Bacillusanthracis] UniRef100_Q81NL1 Bacillus YbcF anthracis 792 Hypotheticalprotein ybcD [Bacillus UniRef100_Q639F7 Bacillus cereus YbcD cereus ZK]ZK 793 Potential NADH-quinone UniRef100_P39755 Bacillus subtilis NdhFoxidoreductase subunit 5 [Bacillus subtilis] 794 YbcI protein [Bacillussubtilis] UniRef100_O34380 Bacillus subtilis YbcI 795 YraA 796TPP-dependent acetoin dehydrogenase UniRef100_Q81PM6 Bacillus AcoA E1alpha-subunit [Bacillus anthracis] anthracis 797 TPP-dependent acetoindehydrogenase UniRef100_Q736U7 Bacillus cereus AcoB E1 beta-subunit[Bacillus cereus] 798 Dihydrolipoyllysine-residue UniRef100_O31550Bacillus subtilis AcoC acetyltransferase component of acetoin cleavingsystem [Bacillus subtilis] 799 Dihydrolipoyl dehydrogenase [BacillusUniRef100_O34324 Bacillus subtilis AcoL subtilis] 800 Acetoin operontranscriptional activator, UniRef100_Q736V6 Bacillus cereus AcoRputative [Bacillus cereus] 801 Hypothetical UPF0060 protein yfjFUniRef100_O31553 Bacillus subtilis YfjF [Bacillus subtilis] 802Maltose-6′-phosphate glucosidase UniRef100_P54716 Bacillus subtilis MalA[Bacillus subtilis] 803 HTH-type transcriptional regulator glvRUniRef100_P54717 Bacillus subtilis YfiA [Bacillus subtilis] 804 PTSsystem, arbutin-like IIBC UniRef100_P54715 Bacillus subtilis MalPcomponent [Bacillus subtilis] 805 UPI00003651CF UniRef100 entryUniRef100_UPI00003651CF 806 UPI000034AA1D UniRef100 entryUniRef100_UPI000034AA1D 807 Transcriptional regulator, MarR familyUniRef100_Q638I2 Bacillus cereus YvaP [Bacillus cereus ZK] ZK 808 YfiD809 Hypothetical protein yfiE [Bacillus UniRef100_P54721 Bacillussubtilis YfiE subtilis] 810 Xylosidase/arabinosidase [BacteroidesUniRef100_Q8A036 Bacteroides XynB thetaiotaomicron] thetaiotaomicron 811Xylan beta-1,4-xylosidase [Bacillus UniRef100_Q9K6P5 Bacillus XynBhalodurans] halodurans 812 Trancriptional regulator of AraC familyUniRef100_Q97FW8 Clostridium YbfI [Clostridium acetobutylicum]acetobutylicum 813 YtcQ 814 NAD(P)H dehydrogenase, quinoneUniRef100_Q638S8 Bacillus cereus ZK family [Bacillus cereus ZK] 815Mutator MutT protein [Bacillus UniRef100_Q9K8B7 Bacillus YjhBhalodurans] halodurans 816 YfiT protein [Bacillus subtilis]UniRef100_O31562 Bacillus subtilis YfiT 817 YfiX [Bacillus subtilis]UniRef100_O52961 Bacillus subtilis YfiX 818 Hypothetical protein yfhB[Bacillus UniRef100_O31570 Bacillus subtilis YfhB subtilis] 819 YfhCprotein [Bacillus subtilis] UniRef100_O31571 Bacillus subtilis YfhC 820Hypothetical protein yfhD [Bacillus UniRef100_O31572 Bacillus subtilissubtilis] 821 822 BH0923 homolog [Bacillus cereus] UniRef100_Q81IA0Bacillus cereus 823 Hypothetical UPF0105 protein yfhF UniRef100_O31574Bacillus subtilis YfhF [Bacillus subtilis] 824 Regulatory protein recX[Bacillus UniRef100_O31575 Bacillus subtilis YfhG subtilis] 825 YfhHprotein [Bacillus subtilis] UniRef100_O31576 Bacillus subtilis YfhH 826827 YfhJ protein [Bacillus subtilis] UniRef100_O31578 Bacillus subtilis828 CsbB protein [Bacillus subtilis] UniRef100_Q45539 Bacillus subtilisCsbB 829 Hypothetical protein SE1997 UniRef100_Q8CR87 Staphylococcusepidermidis [Staphylococcus epidermidis] 830 YfhO protein [Bacillussubtilis] UniRef100_O31582 Bacillus subtilis YfhO 831 YfhP protein[Bacillus subtilis] UniRef100_O31583 Bacillus subtilis YfhP 832 YfhQprotein [Bacillus subtilis] UniRef100_O31584 Bacillus subtilis YfhQ 833YfhS protein [Bacillus subtilis] UniRef100_O31585 Bacillus subtilis 834Unidentfied dehydrogenase [Bacillus UniRef100_P71079 Bacillus subtilisFabL subtilis] 835 836 Hypothetical protein ygaB [BacillusUniRef100_P71080 Bacillus subtilis subtilis] 837 YgaC protein [Bacillussubtilis] UniRef100_Q796Z1 Bacillus subtilis YgaC 838 Unidentifiedtransporter-ATP binding UniRef100_P71082 Bacillus subtilis YgaD[Bacillus subtilis] 839 Oligopeptide ABC transporter [BacillusUniRef100_Q9K6T0 Bacillus AppD halodurans] halodurans 840 OligopeptideABC transporter [Bacillus UniRef100_Q9K6T1 Bacillus AppF halodurans]halodurans 841 Dipeptide transporter protein DppA UniRef100_P94310Bacillus firmus OppA [Bacillus firmus] 842 Dipeptide ABC transporter[Bacillus UniRef100_Q9K6T3 Bacillus AppB halodurans] halodurans 843Dipeptide transport system permease UniRef100_P94312 Bacillus AppCprotein dppC [Bacillus pseudofirmus] pseudofirmus 844 Hypothetical 40.7kd protein [Bacillus UniRef100_P71083 Bacillus subtilis YgaE subtilis]845 Glutamate-1-semialdehyde 2,1- UniRef100_P71084 Bacillus subtilisGsaB aminomutase 2 [Bacillus subtilis] 846 YgaF protein [Bacillussubtilis] UniRef100_Q796Y8 Bacillus subtilis YgaF 847 Peroxide operonregulator [Bacillus UniRef100_P71086 Bacillus subtilis PerR subtilis]848 849 Hypothetical protein ygxA [Bacillus UniRef100_Q04385 Bacillussubtilis YgxA subtilis] 850 RapD 851 852 853 854 855 856 Hypotheticalprotein [Bacillus UniRef100_Q6HH72 Bacillus thuringiensis thuringiensis]857 YxiD 858 Hypothetical protein [uncultured UniRef100_Q64DF2uncultured archaeon archaeon GZfos18F2] GZfos18F2 859 860 YxiD 861 RapE862 Putative membrane protein UniRef100_Q82NM0 Streptomyces avermitilis[Streptomyces avermitilis] 863 3-dehydroquinate dehydratase [ListeriaUniRef100_Q8Y9N4 Listeria AroC monocytogenes] monocytogenes 864 ThiC 865Putative aliphatic sulfonates transport UniRef100_P97027 Bacillussubtilis SsuB ATP-binding protein ssuB [Bacillus subtilis] 866 Putativealiphatic sulfonates binding UniRef100_P40400 Bacillus subtilis SsuAprotein precursor [Bacillus subtilis] 867 Putative aliphatic sulfonatestransport UniRef100_P40401 Bacillus subtilis SsuC permease protein ssuC[Bacillus subtilis] 868 SsuD 869 SsuD 870 Hypothetical lipoprotein ygaOprecursor UniRef100_P97029 Bacillus subtilis YgaO [Bacillus subtilis]871 DNA-binding protein [Bacillus anthracis] UniRef100_Q81V18 Bacillusanthracis 872 873 ABC-type multidrug transport system, UniRef100_Q8RBH0Thermoanaerobacter YhaQ ATPase component tengcongensis[Thermoanaerobacter tengcongensis] 874 875 876 877 878 30S ribosomalprotein S14-2 [Bacillus UniRef100_O31587 Bacillus subtilis subtilis] 879Hypothetical protein yhzB [Bacillus UniRef100_O31588 Bacillus subtilisYhzB subtilis] 880 Hypothetical 48.5 kd protein [BacillusUniRef100_P97030 Bacillus subtilis YhbA subtilis] 881 Hypothetical 35.8kd protein [Bacillus UniRef100_P97031 Bacillus subtilis YhbB subtilis]882 CspR [Bacillus subtilis] UniRef100_Q45512 Bacillus subtilis CspR 883Hypothetical 27 kd protein [Bacillus UniRef100_P97032 Bacillus subtilisYhbD subtilis] 884 Hypothetical Cytosolic Protein [BacillusUniRef100_Q813H1 Bacillus cereus YhbE cereus] 885 Hypothetical protein[Bacillus anthracis] UniRef100_Q81PD2 Bacillus YhbF anthracis 886 887PrkA protein [Bacillus subtilis] UniRef100_P39134 Bacillus subtilis PrkA888 Stress response UPF0229 protein yhbH UniRef100_P45742 Bacillussubtilis YhbH [Bacillus subtilis] 889 YhbJ protein [Bacillus subtilis]UniRef100_O31593 Bacillus subtilis YhbJ 890 Hypothetical transportprotein yhcA UniRef100_P54585 Bacillus subtilis YhcA [Bacillus subtilis]891 Hypothetical protein yhcB [Bacillus UniRef100_P54586 Bacillussubtilis YhcB subtilis] 892 YhcC 893 894 Hypothetical ABC transporterATP- UniRef100_P54591 Bacillus subtilis binding protein yhcG [Bacillussubtilis] 895 Hypothetical ABC transporter ATP- UniRef100_P54592Bacillus subtilis YhcH binding protein yhcH [Bacillus subtilis] 896Hypothetical protein yhcI [Bacillus UniRef100_P54593 Bacillus subtilisYhcI subtilis] 897 Cold shock protein cspB [Bacillus UniRef100_P32081Bacillus subtilis subtilis] 898 ABC transporter, ATP-binding proteinUniRef100_Q6HP89 Bacillus YusC [Bacillus thuringiensis] thuringiensis899 MW0417 protein [Staphylococcus UniRef100_Q8NY20 Staphylococcus YusBaureus] aureus 900 ABC transporter substrate-binding UniRef100_Q81IN6Bacillus cereus YhcJ protein [Bacillus cereus] 901 Hypotheticalsymporter yhcL [Bacillus UniRef100_P54596 Bacillus subtilis YhcLsubtilis] 902 Hypothetical protein yhcM [Bacillus UniRef100_P54597Bacillus subtilis YhcM subtilis] 903 Acylamino-acid-releasing enzymeUniRef100_Q8CXN6 Oceanobacillus YuxL [Oceanobacillus iheyensis]iheyensis 904 Lipoprotein yhcN precursor [Bacillus UniRef100_P54598Bacillus subtilis YhcN subtilis] 905 Hypothetical protein yhcP [BacillusUniRef100_P54600 Bacillus subtilis YhcP subtilis] 906 Hypotheticalprotein yhcQ [Bacillus UniRef100_P54601 Bacillus subtilis YhcQ subtilis]907 Two-component sensor histidine kinase UniRef100_O31661 Bacillussubtilis KinE [Bacillus subtilis] 908 Hypothetical protein yhcRprecursor UniRef100_P54602 Bacillus subtilis YhcR [Bacillus subtilis]909 Hypothetical protein yhcS [Bacillus UniRef100_P54603 Bacillussubtilis YhcS subtilis] 910 Hypothetical pseudouridine synthaseUniRef100_P54604 Bacillus subtilis YhcT yhcT [Bacillus subtilis] 911Hypothetical protein yhcU [Bacillus UniRef100_P54605 Bacillus subtilisYhcU subtilis] 912 Hypothetical protein yhcV [Bacillus UniRef100_P54606Bacillus subtilis YhcV subtilis] 913 Hypothetical protein yhcW [BacillusUniRef100_P54607 Bacillus subtilis YhcW subtilis] 914 HypotheticalUPF0012 protein yhcX UniRef100_P54608 Bacillus subtilis YhcX [Bacillussubtilis] 915 ABC transporter [Bacillus halodurans] UniRef100_Q9KBA3Bacillus YdiF halodurans 916 Hypothetical transport protein yrhGUniRef100_O05399 Bacillus subtilis YrhG [Bacillus subtilis] 917 Lin0826protein [Listeria innocua] UniRef100_Q92DI7 Listeria innocua YwkB 918Alcohol dehydrogenase [Bacillus UniRef100_Q81CT4 Bacillus cereus YogAcereus] 919 Glycerol uptake operon antiterminator UniRef100_P30300Bacillus subtilis GlpP regulatory protein [Bacillus subtilis] 920Glycerol uptake facilitator protein UniRef100_P18156 Bacillus subtilisGlpF [Bacillus subtilis] 921 Glycerol kinase [Bacillus subtilis]UniRef100_P18157 Bacillus subtilis GlpK 922 Aerobic glycerol-3-phosphateUniRef100_P18158 Bacillus subtilis GlpD dehydrogenase [Bacillussubtilis] 923 Alpha-phosphoglucomutase [Bacillus UniRef100_Q68VA2Bacillus subtilis YhxB subtilis subsp. subtilis] subsp. subtilis 924Hypothetical conserved protein UniRef100_Q8ELS7 Oceanobacillus YcgB[Oceanobacillus iheyensis] iheyensis 925 Transcriptional regulatorUniRef100_Q8ELS8 Oceanobacillus iheyensis [Oceanobacillus iheyensis] 926YhcY 927 Hypothetical protein yhcZ [Bacillus UniRef100_O07528 Bacillussubtilis YhcZ subtilis] 928 Hypothetical protein yhdA [BacillusUniRef100_O07529 Bacillus subtilis YhdA subtilis] 929 930 931Hypothetical UPF0074 protein yhdE UniRef100_O07573 Bacillus subtilisYhdE [Bacillus subtilis] 932 Flavohemoprotein [Bacillus halodurans]UniRef100_Q9RC40 Bacillus Hmp halodurans 933 Stage V sporulation proteinR [Bacillus UniRef100_P37875 Bacillus subtilis SpoVR subtilis] 934Probable endopeptidase lytE precursor UniRef100_P54421 Bacillus subtilisLytE [Bacillus subtilis] 935 CitR 936 CitA 937 Glucose dehydrogenase-B[Bacillus UniRef100_Q9Z9R3 Bacillus halodurans halodurans] 938Hypothetical oxidoreductase yhdF UniRef100_O07575 Bacillus subtilis YhdF[Bacillus subtilis] 939 Hypothetical protein yhdH [BacillusUniRef100_O07577 Bacillus subtilis YhdH subtilis] 9402,4-diaminobutyrate decarboxylase UniRef100_Q9KFB9 Bacillus halodurans[Bacillus halodurans] 941 YdeE protein [Bacillus subtilis]UniRef100_P96662 Bacillus subtilis YdeE 942 YdeL protein [Bacillussubtilis] UniRef100_P96669 Bacillus subtilis YdeL 943 BH1582 protein[Bacillus halodurans] UniRef100_Q9KCI9 Bacillus YhdJ halodurans 944 YhdK[Bacillus subtilis subsp. spizizenii] UniRef100_Q7X2K9 Bacillus subtilissubsp. spizizenii 945 YhdL [Bacillus subtilis subsp. spizizenii]UniRef100_Q7X2L0 Bacillus subtilis YhdL subsp. spizizenii 946Hypothetical protein yhdM (RNA UniRef100_O07582 Bacillus subtilis SigMpolymerase ECF(Extracytoplasmic function)-type sigma factor) [Bacillussubtilis] 947 YrkC 948 Hypothetical protein yhdO [BacillusUniRef100_O07584 Bacillus subtilis YhdO subtilis] 949 950 Acyl-CoAthioesterase 1 [Clostridium UniRef100_Q97DR5 Clostridium acetobutylicumacetobutylicum] 951 UPI00003CB259 UniRef100 entryUniRef100_UPI00003CB259 YvkB 952 YhdP 953 HTH-type transcriptionalregulator cueR UniRef100_O07586 Bacillus subtilis YhdQ [Bacillussubtilis] 954 YhdT 955 956 BH3511 protein [Bacillus halodurans]UniRef100_Q9K762 Bacillus halodurans 957 Sporulation specificN-acetylmuramoyl- UniRef100_Q8CX69 Oceanobacillus CwlC L-alanine amidase[Oceanobacillus iheyensis iheyensis] 958 Protein crcB homolog 1[Bacillus UniRef100_O07590 Bacillus subtilis YhdU subtilis] 959 ProteincrcB homolog 2 [Bacillus UniRef100_O07591 Bacillus subtilis YhdVsubtilis] 960 YhdW 961 962 Hypothetical UPF0003 protein yhdYUniRef100_O07594 Bacillus subtilis YhdY [Bacillus subtilis] 963NAD-dependent deacetylase [Bacillus UniRef100_O07595 Bacillus subtilisYhdZ subtilis] 964 965 Hypothetical protein yheN [BacillusUniRef100_O07596 Bacillus subtilis YheN subtilis] 966 Dat 967 Na(+)/H(+)antiporter [Bacillus subtilis] UniRef100_O07553 Bacillus subtilis NhaC968 Hypothetical protein yoxA [Bacillus UniRef100_P39840 Bacillussubtilis YoxA subtilis] 969 Hypothetical protein ydhH [BacillusUniRef100_O05500 Bacillus subtilis YdhH subtilis] 970 Hypotheticalprotein [Bacillus cereus UniRef100_Q63EB4 Bacillus cereus ZK ZK] 971Hypothetical protein [Bacillus cereus] UniRef100_Q81GF4 Bacillus cereus972 Stress response protein nhaX [Bacillus UniRef100_O07552 Bacillussubtilis NhaX subtilis] 973 Hypothetical protein yheI [BacillusUniRef100_O07550 Bacillus subtilis YheI subtilis] 974 Hypotheticalprotein yheH [Bacillus UniRef100_O07549 Bacillus subtilis YheH subtilis]975 Hypothetical protein yheG [Bacillus UniRef100_O07548 Bacillussubtilis YheG subtilis] 976 Small, acid-soluble spore protein BUniRef100_P04832 Bacillus subtilis [Bacillus subtilis] 977 BH1139protein [Bacillus halodurans] UniRef100_Q9KDS2 Bacillus halodurans 978Sugar ABC transporter ATP-binding UniRef100_Q8CUH3 Oceanobacillus MsmXprotein [Oceanobacillus iheyensis] iheyensis 979 Fis-typehelix-turn-helix domain protein UniRef100_Q73A84 Bacillus cereus YxkF[Bacillus cereus] 980 Hypothetical protein yheE [BacillusUniRef100_O07546 Bacillus subtilis subtilis] 981 Hypothetical proteinyheD [Bacillus UniRef100_O07545 Bacillus subtilis YheD subtilis] 982Hypothetical protein yheC [Bacillus UniRef100_O07544 Bacillus subtilisYheC subtilis] 983 Hypothetical protein yheB [Bacillus UniRef100_O07543Bacillus subtilis YheB subtilis] 984 Hypothetical protein yheA [BacillusUniRef100_O07542 Bacillus subtilis YheA subtilis] 985 Stress responseprotein yhaX [Bacillus UniRef100_O07539 Bacillus subtilis YhaX subtilis]986 HemZ 987 YhaR protein [Bacillus subtilis] UniRef100_O07533 Bacillussubtilis YhaR 988 Response regulator aspartate UniRef100_P96649 Bacillussubtilis RapI phosphatase I [Bacillus subtilis] 989 990 Hypotheticalprotein yhaQ [Bacillus UniRef100_O07524 Bacillus subtilis YhaQ subtilis]991 Hypothetical protein yhaP [Bacillus UniRef100_O07523 Bacillussubtilis YhaP subtilis] 992 YhaO 993 Hypothetical protein yhaN [BacillusUniRef100_O08455 Bacillus subtilis YhaN subtilis] 994 YhaM 995Hypothetical protein yhaL [Bacillus UniRef100_O07520 Bacillus subtilissubtilis] 996 Foldase protein prsA precursor UniRef100_P24327 Bacillussubtilis PrsA [Bacillus subtilis] 997 998 Hypothetical protein yhaK[Bacillus UniRef100_O07519 Bacillus subtilis subtilis] 999 Hypotheticalprotein yhaI [Bacillus UniRef100_O07517 Bacillus subtilis YhaI subtilis]1000 Protease production regulatory protein UniRef100_P11065 Bacillussubtilis Hpr hpr [Bacillus subtilis] 1001 Hypothetical protein yhaH[Bacillus UniRef100_O07516 Bacillus subtilis YhaH subtilis] 1002Probable tryptophan transport protein UniRef100_O07515 Bacillus subtilisYhaG [Bacillus subtilis] 1003 Phosphoserine aminotransferaseUniRef100_P80862 Bacillus subtilis SerC [Bacillus subtilis] 1004 Hitprotein [Bacillus subtilis] UniRef100_O07513 Bacillus subtilis Hit 10051006 ABC-type transporter ATP-binding UniRef100_P55339 Bacillus subtilisEcsA protein ecsA [Bacillus subtilis] 1007 Protein ecsB [Bacillussubtilis] UniRef100_P55340 Bacillus subtilis EcsB 1008 Protein ecsC[Bacillus subtilis] UniRef100_P55341 Bacillus subtilis EcsC 1009 YhaAprotein [Bacillus subtilis] UniRef100_O07598 Bacillus subtilis YhaA 1010Hypothetical protein yhfA [Bacillus UniRef100_O07599 Bacillus subtilisYhfA subtilis] 1011 Hypothetical protein yhgC [Bacillus UniRef100_P38049Bacillus subtilis YhgC subtilis] 1012 Penicillin-binding protein 1F[Bacillus UniRef100_P38050 Bacillus subtilis PbpF subtilis] 1013Uroporphyrinogen decarboxylase UniRef100_P32395 Bacillus subtilis HemE[Bacillus subtilis] 1014 Ferrochelatase [Bacillus subtilis]UniRef100_P32396 Bacillus subtilis HemH 1015 Protoporphyrinogen oxidase[Bacillus UniRef100_P32397 Bacillus subtilis HemY subtilis] 1016 YhgD1017 Hypothetical protein yhgE [Bacillus UniRef100_P32399 Bacillussubtilis YhgE subtilis] 1018 3-oxoacyl-[acyl-carrier-protein]UniRef100_O07600 acyl-carrier- FabHB synthase III protein 2 [Bacillussubtilis] protein 1019 Hypothetical protein yhfE [BacillusUniRef100_O07603 Bacillus subtilis YhfE subtilis] 1020 1021 Hypotheticalprotein yhfG [Bacillus UniRef100_O07605 Bacillus subtilis GltT subtilis]1022 Hypothetical protein yhfI [Bacillus UniRef100_O07607 Bacillussubtilis YhfI subtilis] 1023 Hypothetical protein yhfJ [BacillusUniRef100_O07608 Bacillus subtilis YhfJ subtilis] 1024 Hypotheticalprotein yhfL [Bacillus UniRef100_O07610 Bacillus subtilis YhfL subtilis]1025 Hypothetical protein yhfM precursor UniRef100_O07611 Bacillussubtilis YhfM [Bacillus subtilis] 1026 BH2909 protein [Bacillushalodurans] UniRef100_Q9K8U3 Bacillus halodurans 1027 Branched-chainamino acid transporter UniRef100_Q9K8U2 Bacillus AzlC [Bacillushalodurans] halodurans 1028 BH2911 protein [Bacillus halodurans]UniRef100_Q9K8U1 Bacillus halodurans 1029 Putative metalloprotease yhfN[Bacillus UniRef100_P40769 Bacillus subtilis YhfN subtilis] 1030 AprE1031 Transporter, drug/metabolite exporter UniRef100_Q63D40 Bacilluscereus YdeD family [Bacillus cereus ZK] ZK 1032 Hypothetical proteinyhfQ [Bacillus UniRef100_O07616 Bacillus subtilis YhfQ subtilis] 1033YfmD protein [Bacillus subtilis] UniRef100_O34933 Bacillus subtilis YfmD1034 YfmE protein [Bacillus subtilis] UniRef100_O34832 Bacillus subtilisYfmE 1035 Hypothetical protein yhfR [Bacillus UniRef100_O07617 Bacillussubtilis YhfR subtilis] 1036 Heme-based aerotactic transducerUniRef100_O07621 Bacillus subtilis HemAT hemAT [Bacillus subtilis] 1037Rieske 2Fe—2S iron-sulfur protein, UniRef100_Q73E94 Bacillus cereus YhfWputative [Bacillus cereus] 1038 YhxC 1039 Hypothetical protein yhzC[Bacillus UniRef100_O31594 Bacillus subtilis subtilis] 1040 ComK 1041YhjD 1042 YhjE 1043 Signal peptidase I V [Bacillus subtilis]UniRef100_O07560 Bacillus subtilis SipV 1044 Minor extracellularprotease epr UniRef100_P16396 Bacillus subtilis Epr precursor [Bacillussubtilis] 1045 Putative permease [Klebsiella UniRef100_Q765R6 KlebsiellaYybO pneumoniae] pneumoniae 1046 Hypothetical protein [EnterococcusUniRef100_Q82ZQ4 Enterococcus PucR faecalis] faecalis 1047 Putativeallantoinase [Staphylococcus UniRef100_Q9EV52 Staphylococcus PucHxylosus] xylosus 1048 Peptidase, M20/M25/M40 family UniRef100_Q82ZQ2Enterococcus YurH [Enterococcus faecalis] faecalis 1049 Hypotheticalprotein STY0574 UniRef100_Q8XFX7 Salmonella typhi [Salmonella typhi]1050 Ureidoglycolate dehydrogenase UniRef100_Q838P9 Enterococcus YjmC[Enterococcus faecalis] faecalis 1051 Ureidoglycolate dehydrogenaseUniRef100_Q838P9 Enterococcus YjmC [Enterococcus faecalis] faecalis 1052Hypothetical protein [Enterococcus UniRef100_Q838Q3 Enterococcus SucDfaecalis] faecalis 1053 Hypothetical protein [EnterococcusUniRef100_Q838Q3 Enterococcus faecalis faecalis] 1054 1055 Carbamatekinase [Clostridium tetani] UniRef100_Q890W1 Clostridium tetani 1056Major facilitator family transporter UniRef100_Q838Q1 Enterococcus YcbE[Enterococcus faecalis] faecalis 1057 Hypothetical protein yjfAprecursor UniRef100_O34554 Bacillus subtilis [Bacillus subtilis] 1058Response regulator aspartate UniRef100_O32294 Bacillus subtilis RapGphosphatase G [Bacillus subtilis] 1059 1060 1061 1062 Lacl-familytranscription regulator UniRef100_O34829 Bacillus subtilis MsmR[Bacillus subtilis] 1063 Multiple sugar-binding protein [BacillusUniRef100_O34335 Bacillus subtilis MsmE subtilis] 1064 Sugar transporter[Bacillus subtilis] UniRef100_O34706 Bacillus subtilis AmyD 1065 Sugartransporter [Bacillus subtilis] UniRef100_O34518 Bacillus subtilis AmyC1066 Alpha-galactosidase [Bacillus subtilis] UniRef100_O34645 Bacillussubtilis MelA 1067 Hypothetical protein yhjN [Bacillus UniRef100_O07568Bacillus subtilis YhjN subtilis] 1068 Spore coat-associated protein JAUniRef100_Q63FK5 Bacillus cereus ZK [Bacillus cereus ZK] 1069 CotJBprotein [Bacillus subtilis] UniRef100_Q45537 Bacillus subtilis 1070CotJC protein [Bacillus subtilis] UniRef100_Q45538 Bacillus subtilisCotJC 1071 Long-chain fatty-acid-CoA ligase UniRef100_Q9KDT0 BacillusYngI [Bacillus halodurans] halodurans 1072 Hypothetical protein yhjO[Bacillus UniRef100_O07569 Bacillus subtilis YhjO subtilis] 1073Hypothetical protein [Bacillus cereus UniRef100_Q63FZ3 Bacillus cereusLytB ZK] ZK 1074 Sensor histidine kinase [Bacillus UniRef100_Q6HNG3Bacillus PhoR thuringiensis] thuringiensis 1075 Two-component responseregulator UniRef100_Q81I36 Bacillus cereus YclJ [Bacillus cereus] 1076YhjR 1077 Putative molybdate binding protein, UniRef100_O32208 Bacillussubtilis YvgL YvgL [Bacillus subtilis] 1078 Putative molybdate transportprotein, UniRef100_O32209 Bacillus subtilis YvgM YvgM [Bacillussubtilis] 1079 ATP-dependent nuclease subunit B UniRef100_P23477Bacillus subtilis AddB [Bacillus subtilis] 1080 ATP-dependent nucleasesubunit A UniRef100_P23478 Bacillus subtilis AddA [Bacillus subtilis]1081 Exonuclease sbcD homolog [Bacillus UniRef100_P23479 Bacillussubtilis SbcD subtilis] 1082 Nuclease sbcCD subunit C [BacillusUniRef100_O06714 Bacillus subtilis YirY subtilis] 1083 Probable sporegermination protein UniRef100_O06716 Bacillus subtilis gerPF [Bacillussubtilis] 1084 Probable spore germination protein UniRef100_O06717Bacillus subtilis GerPE gerPE [Bacillus subtilis] 1085 Probable sporegermination protein UniRef100_O06718 Bacillus subtilis gerPD [Bacillussubtilis] 1086 Probable spore germination protein UniRef100_O06719Bacillus subtilis GerPC gerPC [Bacillus subtilis] 1087 Probable sporegermination protein UniRef100_O06720 Bacillus subtilis gerPB [Bacillussubtilis] 1088 Probable spore germination protein UniRef100_O06721Bacillus subtilis gerPA [Bacillus subtilis] 1089 Hypothetical proteinyitR [Bacillus UniRef100_O06753 Bacillus subtilis subtilis] 1090 1091Spore coat protein H [Bacillus cereus] UniRef100_Q81EE9 Bacillus cereusCotH 1092 CotG 1093 YisK [Bacillus subtilis] UniRef100_O06724 Bacillussubtilis YisK 1094 Hypothetical protein yloQ [Bacillus UniRef100_Q63CF2Bacillus cereus YloQ cereus ZK] ZK 1095 YisL [Bacillus subtilis]UniRef100_O06725 Bacillus subtilis YisL 1096 Hypothetical protein yisN[Bacillus UniRef100_O06727 Bacillus subtilis YisN subtilis] 1097Asparagine synthetase [glutamine- UniRef100_O05272 glutamine- AsnOhydrolyzing] 3 [Bacillus subtilis] hydrolyzing 1098 NrgA 1099 Nitrogenregulatory PII protein UniRef100_Q8ERT8 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 1100 YisQ [Bacillus subtilis]UniRef100_O07940 Bacillus subtilis YisQ 1101 Putative HTH-typetranscriptional UniRef100_P40331 Bacillus subtilis YisR regulator yisR[Bacillus subtilis] 1102 Acetyltransferase, GNAT family UniRef100_Q63C80Bacillus cereus YokL [Bacillus cereus ZK] ZK 1103 HTH-typetranscriptional regulator degA UniRef100_P37947 Bacillus subtilis DegA[Bacillus subtilis] 1104 Hypothetical oxidoreductase yisSUniRef100_P40332 Bacillus subtilis YisS [Bacillus subtilis] 1105 YisVprotein [Bacillus subtilis] UniRef100_Q796Q6 Bacillus subtilis YisV 1106Diaminobutyrate--pyruvate UniRef100_Q9K9M1 Bacillus GabT transaminase[Bacillus halodurans] halodurans 1107 L-2,4-diaminobutyratedecarboxylase UniRef100_Q8YZR2 Anabaena sp. [Anabaena sp.] 1108 AII0394protein [Anabaena sp.] UniRef100_Q8YZR3 Anabaena sp. 1109 BH2621 protein[Bacillus halodurans] UniRef100_Q9K9M4 Bacillus halodurans 1110 BH2620protein [Bacillus halodurans] UniRef100_Q9K9M5 Bacillus halodurans 1111BH2618 protein [Bacillus halodurans] UniRef100_Q9K9M7 Bacillushalodurans 1112 YitI protein [Bacillus subtilis] UniRef100_O06744Bacillus subtilis YitI 1113 Glr2355 protein [Gloeobacter violaceus]UniRef100_Q7NI29 Gloeobacter YcdF violaceus 1114 BH0411 protein[Bacillus halodurans] UniRef100_Q9KFR6 Bacillus YobV halodurans 11155-methyltetrahydrofolate S- UniRef100_Q9KCE1 Bacillus YitJ homocysteinemethyltransferase halodurans [Bacillus halodurans] 1116 YitJ [Bacillussubtilis] UniRef100_O06745 Bacillus subtilis YitJ 1117 HypotheticalUPF0234 protein yitk UniRef100_O06746 Bacillus subtilis YitK [Bacillussubtilis] 1118 YitL protein [Bacillus subtilis] UniRef100_O06747Bacillus subtilis YitL 1119 1120 1121 Hypothetical UPF0230 protein yitSUniRef100_P70945 Bacillus subtilis YitS [Bacillus subtilis] 1122Hypothetical protein yitT [Bacillus UniRef100_P39803 Bacillus subtilisYitT subtilis] 1123 Intracellular proteinase inhibitor UniRef100_P39804Bacillus subtilis Ipi [Bacillus subtilis] 1124 GMP reductase [Bacillussubtilis] UniRef100_O05269 Bacillus subtilis GuaC 1125 1126 1127Putative orf protein [Bacillus subtilis] UniRef100_P70947 Bacillussubtilis YitU 1128 Putative orf protein [Bacillus subtilis]UniRef100_P70948 Bacillus subtilis YitV 1129 YitW 1130N-acetyl-gamma-glutamyl-phosphate UniRef100_P23715 Bacillus subtilisArgC reductase [Bacillus subtilis] 1131 Arginine biosynthesisbifunctional UniRef100_Q9ZJ14 Includes: ArgJ protein argJ [Includes:Glutamate N- Glutamate N- acetyltransferase (EC 2.3.1.35)acetyltransferase (Ornithine acetyltransferase) (Ornithine (EC 2.3.1.35)transacetylase) (OATase); Amino-acid (Ornithine acetyltransferase (EC2.3.1.1) (N- acetyltransferase) acetylglutamate synthase) (AGS)](Ornithine [Contai transacetylase) (OATase); Amino-acidacetyltransferase (EC 2.3.1.1) (N- acetylglutamate synthase) (AGS) 1132Acetylglutamate kinase [Bacillus UniRef100_P36840 Bacillus subtilis ArgBsubtilis] 1133 Acetylomithine aminotransferase UniRef100_P36839 Bacillussubtilis ArgD [Bacillus subtilis] 1134 Carbamoyl-phosphate synthase,UniRef100_P36838 Bacillus subtilis CarA arginine-specific, small chain[Bacillus subtilis] 1135 Carbamoyl-phosphate synthase, UniRef100_P18185Bacillus subtilis CarB arginine-specific, large chain [Bacillussubtilis] 1136 Ornithine carbamoyltransferase UniRef100_P18186 Bacillussubtilis ArgF [Bacillus subtilis] 1137 Undecaprenyl-diphosphatase 1UniRef100_Q81HV4 Bacillus cereus YubB [Bacillus cereus] 1138 YjzCprotein [Bacillus subtilis] UniRef100_O34585 Bacillus subtilis 1139 1140Hypothetical protein yjaU [Bacillus UniRef100_O35001 Bacillus subtilisYjaU subtilis] 1141 ArgF and med genes, partial and UniRef100_O32435Bacillus subtilis YjaV complete cds [Bacillus subtilis] 1142Transcriptional activator protein med UniRef100_O32436 Bacillus subtilisMed precursor [Bacillus subtilis] 1143 ComG operon repressor [BacillusUniRef100_O32437 Bacillus subtilis subtilis] 1144 Hypothetical proteinyjzB [Bacillus UniRef100_O34891 Bacillus subtilis subtilis] 11453-oxoacyl-[acyl-carrier-protein] UniRef100_O34746 acyl-carrier- FabHAsynthase III protein 1 [Bacillus subtilis] protein 1146Beta-ketoacyl-acyl carrier protein UniRef100_O34340 Bacillus subtilisFabF synthase II [Bacillus subtilis] 1147 YjaZ protein [Bacillussubtilis] UniRef100_O31596 Bacillus subtilis YjaZ 1148 Oligopeptidetransport ATP-binding UniRef100_P42064 Bacillus subtilis AppD proteinappD [Bacillus subtilis] 1149 Oligopeptide transport ATP-bindingUniRef100_P42065 Bacillus subtilis AppF protein appF [Bacillus subtilis]1150 Oligopeptide-binding protein appA UniRef100_P42061 Bacillussubtilis AppA precursor [Bacillus subtilis] 1151 Oligopeptide transportsystem UniRef100_P42062 Bacillus subtilis AppB permease protein appB[Bacillus subtilis] 1152 Oligopeptide transport system UniRef100_P42063Bacillus subtilis AppC permease protein appC [Bacillus subtilis] 1153Permease, putative [Bacillus cereus] UniRef100_Q734Y3 Bacillus cereusYvqJ 1154 YjbA 1155 Tryptophanyl-tRNA synthetase [BacillusUniRef100_P21656 Bacillus subtilis TrpS subtilis] 1156Oligopeptide-binding protein oppA UniRef100_P24141 Bacillus subtilisOppA precursor [Bacillus subtilis] 1157 Oligopeptide transport systemUniRef100_P24138 Bacillus subtilis OppB permease protein oppB [Bacillussubtilis] 1158 Oligopeptide transport system UniRef100_P24139 Bacillussubtilis OppC permease protein oppC [Bacillus subtilis] 1159Oligopeptide transport ATP-binding UniRef100_P24136 Bacillus subtilisOppD protein oppD [Bacillus subtilis] 1160 Oligopeptide transportATP-binding UniRef100_P24137 Bacillus subtilis OppF protein oppF[Bacillus subtilis] 1161 YjbC protein [Bacillus subtilis]UniRef100_O31601 Bacillus subtilis YjbC 1162 Regulatory protein spx[Bacillus subtilis] UniRef100_O31602 Bacillus subtilis YjbD 1163 YjbEprotein [Bacillus subtilis] UniRef100_O31603 Bacillus subtilis YjbE 1164Adapter protein mecA 1 [Bacillus UniRef100_P37958 Bacillus subtilis MecAsubtilis] 1165 Hypothetical conserved protein UniRef100_Q8ELH8Oceanobacillus YflP [Oceanobacillus iheyensis] iheyensis 1166Hypothetical protein OB3248 UniRef100_Q8ELH9 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 1167 Hypothetical conserved proteinUniRef100_Q8ELI0 Oceanobacillus iheyensis [Oceanobacillus iheyensis]1168 Response regulator of citrate/malate UniRef100_Q7ML23 Vibriovulnificus CitT metabolism [Vibrio vulnificus] 1169 Sensor protein citS[Bacillus UniRef100_Q9RC53 Bacillus YufL halodurans] halodurans 1170YjbF protein [Bacillus subtilis] UniRef100_O31604 Bacillus subtilis YjbF1171 YjbG 1172 1173 YjbH protein [Bacillus subtilis] UniRef100_O31606Bacillus subtilis YjbH 1174 YjbI protein [Bacillus subtilis]UniRef100_O31607 Bacillus subtilis YjbI 1175 YjbJ protein [Bacillussubtilis] UniRef100_O31608 Bacillus subtilis YjbJ 1176 YjbK protein[Bacillus subtilis] UniRef100_O31609 Bacillus subtilis YjbK 1177 YjbLprotein [Bacillus subtilis] UniRef100_O31610 Bacillus subtilis YjbL 1178YjbM protein [Bacillus subtilis] UniRef100_O31611 Bacillus subtilis YjbM1179 YjbN 1180 Hypothetical pseudouridine synthase UniRef100_O31613Bacillus subtilis YjbO yjbO [Bacillus subtilis] 1181 YjbP protein[Bacillus subtilis] UniRef100_O31614 Bacillus subtilis YjbP 1182 YjbQprotein [Bacillus subtilis] UniRef100_O31615 Bacillus subtilis YjbQ 1183Transcriptional activator tenA [Bacillus UniRef100_P25052 Bacillussubtilis TenA subtilis] 1184 Regulatory protein tenI [BacillusUniRef100_P25053 Bacillus subtilis TenI subtilis] 1185 Glycine oxidase[Bacillus subtilis] UniRef100_O31616 Bacillus subtilis GoxB 1186 ThiSprotein [Bacillus subtilis] UniRef100_O31617 Bacillus subtilis 1187Thiazole biosynthesis protein thiG UniRef100_O31618 Bacillus subtilisThiG [Bacillus subtilis] 1188 ThiF protein [Bacillus subtilis]UniRef100_O31619 Bacillus subtilis ThiF 1189 YjbV protein [Bacillussubtilis] UniRef100_O31620 Bacillus subtilis YjbV 1190Enoyl-[acyl-carrier-protein] reductase UniRef100_P54616 acyl-carrier-FabI [NADH] [Bacillus subtilis] protein 1191 YjbX protein [Bacillussubtilis] UniRef100_O31622 Bacillus subtilis YjbX 1192 Spore coatprotein Z [Bacillus subtilis] UniRef100_Q08312 Bacillus subtilis CotZ1193 Spore coat protein Y [Bacillus subtilis] UniRef100_Q08311 Bacillussubtilis CotY 1194 Spore coat protein X [Bacillus subtilis]UniRef100_Q08313 Bacillus subtilis CotX 1195 Spore coat protein W[Bacillus subtilis] UniRef100_Q08310 Bacillus subtilis CotW 1196 Sporecoat protein V [Bacillus subtilis] UniRef100_Q08309 Bacillus subtilisCotV 1197 YjcA protein [Bacillus subtilis] UniRef100_O31623 Bacillussubtilis YjcA 1198 1199 1200 YjcC protein [Bacillus subtilis]UniRef100_O31625 Bacillus subtilis 1201 YjcD 1202 YngC 1203 GalE 1204YngB protein [Bacillus subtilis] UniRef100_O31822 Bacillus subtilis YngB1205 YngA protein [Bacillus UniRef100_Q70JY6 Bacillus YngAamyloliquefaciens] amyloliquefaciens 1206 YjcF protein [Bacillussubtilis] UniRef100_O31628 Bacillus subtilis YjcF 1207 YjcG protein[Bacillus subtilis] UniRef100_O31629 Bacillus subtilis YjcG 1208 YjcHprotein [Bacillus subtilis] UniRef100_O31630 Bacillus subtilis YjcH 1209Hypothetical protein [Bacillus cereus] UniRef100_Q739H9 Bacillus cereus1210 BH1889 protein [Bacillus halodurans] UniRef100_Q9KBN6 Bacillus YobVhalodurans 1211 YjcI protein [Bacillus subtilis] UniRef100_O31631Bacillus subtilis YjcI 1212 YjcJ protein [Bacillus subtilis]UniRef100_O31632 Bacillus subtilis YjcJ 1213 YjcL protein [Bacillussubtilis] UniRef100_O31634 Bacillus subtilis YjcL 1214 Transcriptionalregulator, MarR/EmrR UniRef100_Q97DR6 Clostridium acetobutylicum family[Clostridium acetobutylicum] 1215 Penicillin-binding protein 4*[Bacillus UniRef100_P32959 Bacillus subtilis PbpE subtilis] 1216 AbnA1217 1218 Maltose transacetylase [Bacillus UniRef100_Q75TH6 Bacillus Maastearothermophilus] stearothermophilus 1219 1220 Putative HTH-typetranscriptional UniRef100_P39647 Bacillus subtilis YwfK regulator ywfK[Bacillus subtilis] 1221 Sulfite reductase [Bacillus halodurans]UniRef100_Q9KF76 Bacillus YvgR halodurans 1222 Sulfite reductase[Bacillus halodurans] UniRef100_Q9KF75 Bacillus YvgQ halodurans 1223Putative HTH-type transcriptional UniRef100_O34701 Bacillus subtilisYoaU regulator yoaU [Bacillus subtilis] 1224 Hypothetical transportprotein yoaV UniRef100_O34416 Bacillus subtilis YoaV [Bacillus subtilis]1225 Hypothetical protein VPA0302 [Vibrio UniRef100_Q87JF1 Vibrio YyaHparahaemolyticus] parahaemolyticus 1226 Hypothetical protein yoeBprecursor UniRef100_O34841 Bacillus subtilis YoeB [Bacillus subtilis]1227 YocH 1228 Permease, general substrate UniRef100_Q6HMC3 BacillusLmrB transporter [Bacillus thuringiensis] thuringiensis 1229 PutativeHTH-type transcriptional UniRef100_P42105 Bacillus subtilis YxaFregulator yxaF [Bacillus subtilis] 1230 1231 YmzD 1232 YeeF 1233 YjqB1234 Phage-like element PBSX protein xkdA UniRef100_P39780 Bacillussubtilis XkdA [Bacillus subtilis] 1235 HTH-type transcriptionalregulator xre UniRef100_P23789 Bacillus subtilis Xre [Bacillus subtilis]1236 1237 1238 Phage-like element PBSX protein xkdB UniRef100_P39781Bacillus subtilis XkdB [Bacillus subtilis] 1239 Phage-like element PBSXprotein xkdC UniRef100_P39782 Bacillus subtilis XkdC [Bacillus subtilis]1240 Phage-like element PBSX protein xkdD UniRef100_P39783 Bacillussubtilis XkdD [Bacillus subtilis] 1241 Phage-like element PBSX proteinxtrA UniRef100_P54344 Bacillus subtilis [Bacillus subtilis] 1242Positive control factor [Bacillus subtilis] UniRef100_P39784 Bacillussubtilis Xpf 1243 PBSX phage terminase small subunit UniRef100_P39785Bacillus subtilis XtmA [Bacillus subtilis] 1244 PBSX phage terminaselarge subunit UniRef100_P39786 Bacillus subtilis XtmB [Bacillussubtilis] 1245 Phage-like element PBSX protein xkdE UniRef100_P54325Bacillus subtilis XkdE [Bacillus subtilis] 1246 Phage-like element PBSXprotein xkdF UniRef100_P54326 Bacillus subtilis XkdF [Bacillus subtilis]1247 Phage-like element PBSX protein xkdG UniRef100_P54327 Bacillussubtilis XkdG [Bacillus subtilis] 1248 Hypothetical protein yqbG[Bacillus UniRef100_P45923 Bacillus subtilis YqbG subtilis] 1249Hypothetical protein yqbH [Bacillus UniRef100_P45924 Bacillus subtilisYqbH subtilis] 1250 Phage-like element PBSX protein xkdIUniRef100_P54329 Bacillus subtilis XkdI [Bacillus subtilis] 1251Phage-like element PBSX protein xkdJ UniRef100_P54330 Bacillus subtilisXkdJ [Bacillus subtilis] 1252 Lin1277 protein [Listeria innocua]UniRef100_Q92CB2 Listeria innocua 1253 Phage-like element PBSX proteinxkdK UniRef100_P54331 Bacillus subtilis XkdK [Bacillus subtilis] 1254Phage-like element PBSX protein xkdM UniRef100_P54332 Bacillus subtilisXkdM [Bacillus subtilis] 1255 Phage-like element PBSX protein xkdNUniRef100_P54333 Bacillus subtilis XkdN [Bacillus subtilis] 1256Phage-like element PBSX protein xkdO UniRef100_P54334 Bacillus subtilisXkdO [Bacillus subtilis] 1257 Phage-like element PBSX protein xkdPUniRef100_P54335 Bacillus subtilis YqbP [Bacillus subtilis] 1258Hypothetical protein yqbQ [Bacillus UniRef100_P45950 Bacillus subtilisYqbQ subtilis] 1259 Hypothetical protein yqbR [Bacillus UniRef100_P45933Bacillus subtilis YqbR subtilis] 1260 Phage-like element PBSX proteinxkdS UniRef100_P54338 Bacillus subtilis XkdS [Bacillus subtilis] 1261Hypothetical protein yqbT [Bacillus UniRef100_P45935 Bacillus subtilisYqbT subtilis] 1262 Phage-like element PBSX protein xkdUUniRef100_P54340 Bacillus subtilis XkdU [Bacillus subtilis] 1263 1264XkdV 1265 YomR 1266 1267 1268 BlyA 1269 Regulatory protein [BacillusUniRef100_Q9ZFL9 Bacillus YdhC stearothermophilus] stearothermophilus1270 Zinc-containing alcohol dehydrogenase UniRef100_O35045 Bacillussubtilis YjmD [Bacillus subtilis] 1271 Mannonate dehydratase 1 [BacillusUniRef100_Q9KDZ8 Bacillus UxuA halodurans] halodurans 1272 D-mannonateoxidoreductase [Bacillus UniRef100_Q9KDZ4 Bacillus YjmF halodurans]halodurans 1273 UPI00002F2634 UniRef100 entry UniRef100_UPI00002F2634YjmD 1274 Hexuronate transporter [Bacillus UniRef100_O34456 Bacillussubtilis ExuT subtilis] 1275 Stage II sporulation protein SB [BacillusUniRef100_O34800 Bacillus subtilis subtilis] 1276 Stage II sporulationprotein SA [Bacillus UniRef100_O34853 Bacillus subtilis SpoIISAsubtilis] 1277 UPI00003CC121 UniRef100 entry UniRef100_UPI00003CC121 Pit1278 Hypothetical UPF0111 protein ykaA UniRef100_O34454 Bacillussubtilis YkaA [Bacillus subtilis] 1279 Ggt 1280 YesL protein [Bacillussubtilis] UniRef100_O31515 Bacillus subtilis YesL 1281 YesM protein[Bacillus subtilis] UniRef100_O31516 Bacillus subtilis YesM 1282 YesNprotein [Bacillus subtilis] UniRef100_O31517 Bacillus subtilis YesN 1283YesO protein [Bacillus subtilis] UniRef100_O31518 Bacillus subtilis YesO1284 Probable ABC transporter permease UniRef100_O31519 Bacillussubtilis YesP protein yesP [Bacillus subtilis] 1285 Probable ABCtransporter permease UniRef100_O31520 Bacillus subtilis YesQ proteinyesQ [Bacillus subtilis] 1286 YesR protein [Bacillus subtilis]UniRef100_O31521 Bacillus subtilis YesR 1287 YesS protein [Bacillussubtilis] UniRef100_O31522 Bacillus subtilis YesS 1288 YesT protein[Bacillus subtilis] UniRef100_O31523 Bacillus subtilis YesT 1289 YesU1290 YesV protein [Bacillus subtilis] UniRef100_O31525 Bacillus subtilisYesV 1291 YesW protein [Bacillus subtilis] UniRef100_O31526 Bacillussubtilis YesW 1292 1293 YesT 1294 YesX protein [Bacillus subtilis]UniRef100_O31527 Bacillus subtilis YesX 1295 Putative ion-channelprotein UniRef100_Q8Z4X6 Salmonella typhi YccK [Salmonella typhi] 1296YesY protein [Bacillus subtilis] UniRef100_O31528 Bacillus subtilis YesY1297 YesZ protein [Bacillus subtilis] UniRef100_O31529 Bacillus subtilisYesZ 1298 YetA 1299 Lipoprotein lplA precursor [BacillusUniRef100_P37966 Bacillus subtilis LplA subtilis] 1300 LplB protein[Bacillus subtilis] UniRef100_P39128 Bacillus subtilis LplB 1301 LplCprotein [Bacillus subtilis] UniRef100_P39129 Bacillus subtilis LplC 1302YkbA protein [Bacillus subtilis] UniRef100_O34739 Bacillus subtilis YkbA1303 YkcA protein [Bacillus subtilis] UniRef100_O34689 Bacillus subtilisYkcA 1304 Hypothetical protein [Bacillus cereus] UniRef100_Q81CP9Bacillus cereus 1305 Probable serine protease do-like htrAUniRef100_O34358 Bacillus subtilis HtrA [Bacillus subtilis] 1306Pyrroline-5-carboxylate reductase 3 UniRef100_Q00777 Bacillus subtilisProG [Bacillus subtilis] 1307 D-aminopeptidase [Bacillus subtilis]UniRef100_P26902 Bacillus subtilis DppA 1308 Dipeptide transport systempermease UniRef100_P26903 Bacillus subtilis DppB protein dppB [Bacillussubtilis] 1309 Dipeptide transport system permease UniRef100_P26904Bacillus subtilis DppC protein dppC [Bacillus subtilis] 1310 Dipeptidetransport ATP-binding protein UniRef100_P26905 Bacillus subtilis DppDdppD [Bacillus subtilis] 1311 Dipeptide-binding protein dppEUniRef100_P26906 Bacillus subtilis DppE precursor [Bacillus subtilis]1312 Hypothetical protein ykfA [Bacillus UniRef100_O34851 Bacillussubtilis YkfA subtilis] 1313 YkfB [Bacillus subtilis] UniRef100_O34508Bacillus subtilis YkfB 1314 YkfC [Bacillus subtilis] UniRef100_O35010Bacillus subtilis YkfC 1315 YkfD [Bacillus subtilis] UniRef100_O34480Bacillus subtilis YkfD 1316 BH1779 protein [Bacillus halodurans]UniRef100_Q9KBZ5 Bacillus YkgA halodurans 1317 Putative acyl-CoAthioester hydrolase UniRef100_P49851 Bacillus subtilis YkhA ykhA[Bacillus subtilis] 1318 YkjA 1319 Pectate lyase 47 precursor [Bacillussp. UniRef100_Q9AJM4 Bacillus sp. TS- Pel TS-47] 47 1320 Transcriptionalregulator, PadR family UniRef100_Q6HED5 Bacillus thuringiensis [Bacillusthuringiensis] 1321 Hypothetical protein OB0568 UniRef100_Q8ESQ3Oceanobacillus iheyensis [Oceanobacillus iheyensis] 1322 BH1312 protein[Bacillus halodurans] UniRef100_Q9KDA2 Bacillus halodurans 1323Hypothetical protein ykkA [Bacillus UniRef100_P49854 Bacillus subtilisYkkA subtilis] 1324 Hypothetical protein ykkC [Bacillus UniRef100_P49856Bacillus subtilis YkkC subtilis] 1325 1326 YkkE [Bacillus subtilis]UniRef100_O34990 Bacillus subtilis YkkE 1327 Glutamate 5-kinase 1[Bacillus subtilis] UniRef100_P39820 Bacillus subtilis ProB 1328Gamma-glutamyl phosphate reductase UniRef100_P39821 Bacillus subtilisProA [Bacillus subtilis] 1329 Organic hydroperoxide resistanceUniRef100_O34762 Bacillus subtilis YklA protein ohrA [Bacillus subtilis]1330 Organic hydroperoxide resistance UniRef100_O34777 Bacillus subtilisYkmA transcriptional regulator [Bacillus subtilis] 1331 Organichydroperoxide resistance UniRef100_P80242 Bacillus subtilis YkzA proteinohrB [Bacillus subtilis] 1332 1333 Guanine deaminase [Bacillus subtilis]UniRef100_O34598 Bacillus subtilis GuaD 1334 Phosphoglycerate mutase[Bacillus UniRef100_Q9ALU0 Bacillus YhfR stearothermophilus]stearothermophilus 1335 1336 13375-methyltetrahydropteroyltriglutamate-- UniRef100_P80877 Bacillussubtilis MetE homocysteine methyltransferase [Bacillus subtilis] 1338Intracellular serine protease [Bacillus UniRef100_Q69DB4 Bacillus sp.IspA sp. WRD-2] WRD-2 1339 1340 YkoK [Bacillus subtilis]UniRef100_O34442 Bacillus subtilis YkoK 1341 1342 Integrase[Oceanobacillus iheyensis] UniRef100_Q8ETV2 Oceanobacillus YdcLiheyensis 1343 YqaB 1344 Hypothetical protein [Bacillus anthracis]UniRef100_Q81UE0 Bacillus anthracis 1345 YonS 1346 1347 PutativeHTH-type transcriptional UniRef100_P45902 Bacillus subtilis YqaEregulator yqaE [Bacillus subtilis] 1348 Transcriptional regulatorUniRef100_Q7P886 Fusobacterium nucleatum [Fusobacterium nucleatum subsp.subsp. vincentii ATCC 49256 vincentii ATCC 49256] 1349 1350 1351 Lin1236protein [Listeria innocua] UniRef100_Q92CD6 Listeria innocua 1352 13531354 1355 YqaJ 1356 35 protein [Bacteriophage SPP1] UniRef100_Q38143Bacteriophage YqaK SPP1 1357 YqaL 1358 YqaM 1359 Hypothetical proteinyqaO [Bacillus UniRef100_P45912 Bacillus subtilis subtilis] 1360Hypothetical protein yopY UniRef100_O64108 Bacteriophage SPBc2[Bacteriophage SPBc2] 1361 1362 1363 1364 Hypothetical protein CTC02137UniRef100_Q892G2 Clostridium tetani [Clostridium tetani] 1365Hypothetical protein MW1918 UniRef100_Q8NVN5 Staphylococcus YqaN[Staphylococcus aureus] aureus 1366 1367 Single-strand binding protein 2[Listeria UniRef100_Q8Y4X1 Listeria monocytogenes monocytogenes] 13681369 1370 Hypothetical protein yqaQ [Bacillus UniRef100_P45948 Bacillussubtilis YqaQ subtilis] 1371 1372 Hypothetical protein yqaS [BacillusUniRef100_P45915 Bacillus subtilis YqaS subtilis] 1373 Hypotheticalprotein yqaT [Bacillus UniRef100_P45916 Bacillus subtilis YqaT subtilis]1374 Hypothetical phage associated protein UniRef100_Q8K6I0Streptococcus pyogenes SpyM3_1326 [Streptococcus pyogenes] 1375 Minorhead structural component GP7 UniRef100_Q38442 Bacteriophage SPP1[Bacteriophage SPP1] 1376 1377 1378 1379 Hypothetical protein CTC01553UniRef100_Q894J0 Clostridium tetani [Clostridium tetani] 1380 Majorcapsid protein [Bacteriophage UniRef100_Q9T1B7 Bacteriophage A118 A118]1381 ORF28 [Bacteriophage phi-105] UniRef100_Q9ZXF5 Bacteriophagephi-105 1382 1383 15 protein [Bacteriophage SPP1] UniRef100_Q38584Bacteriophage SPP1 1384 Complete nucleotide sequence UniRef100_O48446Bacteriophage SPP1 [Bacteriophage SPP1] 1385 1386 Complete nucleotidesequence UniRef100_O48448 Bacteriophage SPP1 [Bacteriophage SPP1] 1387Complete nucleotide sequence UniRef100_O48449 Bacteriophage SPP1[Bacteriophage SPP1] 1388 1389 Complete nucleotide sequenceUniRef100_O48453 Bacteriophage SPP1 [Bacteriophage SPP1] 1390 1391Complete nucleotide sequence UniRef100_O48455 Bacteriophage XkdO[Bacteriophage SPP1] SPP1 1392 Complete nucleotide sequenceUniRef100_O48459 Bacteriophage SPP1 [Bacteriophage SPP1] 1393 Completenucleotide sequence UniRef100_O48463 Bacteriophage SPP1 [BacteriophageSPP1] 1394 1395 1396 LycA [Clostridium botulinum] UniRef100_Q6RI00Clostridium botulinum 1397 Hypothetical protein yrkC [BacillusUniRef100_P54430 Bacillus subtilis YrkC subtilis] 1398 YhjR 1399 YdfSprotein [Bacillus subtilis] UniRef100_P96697 Bacillus subtilis YdfS 1400HTH-type transcriptional regulator tnrA UniRef100_Q45666 Bacillussubtilis TnrA [Bacillus subtilis] 1401 Hypothetical protein ykzB[Bacillus UniRef100_O34923 Bacillus subtilis subtilis] 1402 1403 YkoM[Bacillus subtilis] UniRef100_O34949 Bacillus subtilis YkoM 1404 YkoUprotein [Bacillus subtilis] UniRef100_O34398 Bacillus subtilis YkoU 1405YkoV protein [Bacillus subtilis] UniRef100_O34859 Bacillus subtilis YkoV1406 Signaling protein ykoW [Bacillus UniRef100_O34311 Bacillus subtilisYkoW subtilis] 1407 YkoX protein [Bacillus subtilis] UniRef100_O34908Bacillus subtilis YkoX 1408 YkoY protein [Bacillus subtilis]UniRef100_O34997 Bacillus subtilis YkoY 1409 RNA polymerase sigma factor[Bacillus UniRef100_O31654 Bacillus subtilis SigI subtilis] 1410 YkrI1411 Small, acid-soluble spore protein C3 UniRef100_P10572 Bacillusmegaterium [Bacillus megaterium] 1412 YkrK protein [Bacillus subtilis]UniRef100_O31656 Bacillus subtilis YkrK 1413 Probable protease htpXhomolog UniRef100_O31657 Bacillus subtilis YkrL [Bacillus subtilis] 1414YkrM protein [Bacillus subtilis] UniRef100_O31658 Bacillus subtilis YkrM1415 Penicillin-binding protein 3 [Bacillus UniRef100_P42971 Bacillussubtilis PbpC subtilis] 1416 Hypothetical protein [Bacillus cereus]UniRef100_Q73BI4 Bacillus cereus 1417 1418 YkrP protein [Bacillussubtilis] UniRef100_O31660 Bacillus subtilis YkrP 1419 Two-componentsensor histidine kinase UniRef100_O31661 Bacillus subtilis KinE[Bacillus subtilis] 1420 Methylated-DNA--protein-cysteineUniRef100_P11742 Bacillus subtilis Ogt methyltransferase [Bacillussubtilis] 1421 1422 Methylthioribose-1-phosphate UniRef100_O31662Bacillus subtilis YkrS isomerase [Bacillus subtilis] 1423Methylthioribose kinase [Bacillus UniRef100_O31663 Bacillus subtilisYkrT subtilis] 1424 YkrU protein [Bacillus subtilis] UniRef100_O31664Bacillus subtilis YkrU 1425 Transaminase mtnE [Bacillus subtilis]UniRef100_O31665 Bacillus subtilis YkrV 14262,3-diketo-5-methylthiopentyl-1- UniRef100_O31666 Bacillus subtilis YkrWphosphate enolase [Bacillus subtilis] 1427Methylthioribulose-1-phosphate UniRef100_O31668 Bacillus subtilis YkrYdehydratase [Bacillus subtilis] 1428 1,2-dihydroxy-3-keto-5-UniRef100_O31669 Bacillus subtilis YkrZ methylthiopentene dioxygenase[Bacillus subtilis] 1429 Metallothiol transferase fosB UniRef100_Q8CXK5Oceanobacillus YndN [Oceanobacillus iheyensis] iheyensis 1430 YkvAprotein [Bacillus subtilis] UniRef100_O31670 Bacillus subtilis 1431Stage 0 sporulation regulatory protein UniRef100_P05043 Bacillussubtilis [Bacillus subtilis] 1432 Two-component sensor histidine kinaseUniRef100_O31671 Bacillus subtilis KinD [Bacillus subtilis] 1433 YkvE1434 Chemotaxis motB protein [Bacillus UniRef100_P28612 Bacillussubtilis MotB subtilis] 1435 Chemotaxis motA protein [BacillusUniRef100_P28611 Bacillus subtilis MotA subtilis] 1436 ATP-dependent Clpprotease-like UniRef100_O31673 Bacillus subtilis ClpE [Bacillussubtilis] 1437 YkvI protein [Bacillus subtilis] UniRef100_O31674Bacillus subtilis YkvI 1438 YkvJ protein [Bacillus subtilis]UniRef100_O31675 Bacillus subtilis YkvJ 1439 YkvK protein [Bacillussubtilis] UniRef100_O31676 Bacillus subtilis YkvK 1440 YkvL protein[Bacillus subtilis] UniRef100_O31677 Bacillus subtilis YkvL 1441 YkvMprotein [Bacillus subtilis] UniRef100_O31678 Bacillus subtilis YkvM 1442DNA integration/recombination protein UniRef100_Q894H7 Clostridiumtetani CodV [Clostridium tetani] 1443 Integrase/recombinase [Bacilluscereus UniRef100_Q633V7 Bacillus cereus RipX ZK] ZK 1444 1445 1446 1447YoqV protein [Bacteriophage SPBc2] UniRef100_O64130 Bacteriophage LigBSPBc2 1448 1449 1450 UPI00003CC586 UniRef100 entryUniRef100_UPI00003CC586 1451 1452 1453 1454 1455 Prophage LambdaBa02,HNH UniRef100_Q81W86 Bacillus anthracis endonuclease family protein[Bacillus anthracis] 1456 Terminase small subunit UniRef100_Q6GAL5Staphylococcus aureus [Staphylococcus aureus] 1457 Prophage LambdaBa02,terminase, UniRef100_Q6HUD2 Bacillus anthracis large subunit, putative[Bacillus anthracis] 1458 Hypothetical protein [Bacillus anthracis]UniRef100_Q81W89 Bacillus anthracis 1459 ClpP family serine protease,possible UniRef100_Q97HW4 Clostridium ClpP phage related [Clostridiumacetobutylicum acetobutylicum] 1460 Prophage LambdaBa02, major capsidUniRef100_Q81W91 Bacillus anthracis protein, putative [Bacillusanthracis] 1461 Precursor polypeptide (AA −37 to 1647) UniRef100_Q03658unidentified bacterium precursor [unidentified bacterium] 1462 Gp7protein [Bacteriophage phi3626] UniRef100_Q8SBP7 Bacteriophage phi36261463 Uncharacterized phage related protein UniRef100_Q97HW7 Clostridiumacetobutylicum [Clostridium acetobutylicum] 1464 Hypothetical proteinCAC1887 UniRef100_Q97HW9 Clostridium acetobutylicum [Clostridiumacetobutylicum] 1465 Prophage LambdaBa02, major tail UniRef100_Q81W97Bacillus anthracis protein, putative [Bacillus anthracis] 1466 1467 1468Prophage LambdaBa02, tape measure UniRef100_Q81WA0 Bacillus YqbOprotein, putative [Bacillus anthracis] anthracis 1469 Lin2382 protein[Listeria innocua] UniRef100_Q928Z8 Listeria innocua 1470 Protein gp18[Listeria monocytogenes] UniRef100_Q8Y4Z4 Listeria monocytogenes 1471YclG 1472 XkdV 1473 XkdW 1474 YomP protein [Bacteriophage SPBc2]UniRef100_O64052 Bacteriophage SPBc2 1475 Glycerophosphoryl diesterUniRef100_Q737E6 Bacillus cereus GlpQ phosphodiesterase, putative[Bacillus cereus] 1476 Protein bhlA [Bacteriophage SPBc2]UniRef100_O64039 Bacteriophage SPBc2 1477 ORF46 [Bacteriophage phi-105]UniRef100_Q9ZXD7 Bacteriophage XlyB phi-105 1478 1479 1480 1481Transcriptional regulator, DeoR family UniRef100_Q816D5 Bacillus cereus[Bacillus cereus] 1482 Hypothetical protein yolD UniRef100_O64030Bacteriophage SPBc2 [Bacteriophage SPBc2] 1483 DNAintegration/recombination/invertion UniRef100_Q81GD4 Bacillus cereusYdcL protein [Bacillus cereus] 1484 YkvM protein [Bacillus subtilis]UniRef100_O31678 Bacillus subtilis 1485 Response regulator aspartateUniRef100_P40771 Bacillus subtilis RapH phosphatase H [Bacillussubtilis] 1486 YoaT [Bacillus subtilis] UniRef100_O34535 Bacillussubtilis YoaT 1487 YozG protein [Bacillus subtilis] UniRef100_O31834Bacillus subtilis 1488 YoaS protein [Bacillus subtilis] UniRef100_O31833Bacillus subtilis YoaS 1489 1490 1491 1492 1493 1494 YkvS protein[Bacillus subtilis] UniRef100_O31684 Bacillus subtilis 1495 BH2327protein [Bacillus halodurans] UniRef100_Q9KAG0 Bacillus halodurans 1496YkvT protein [Bacillus subtilis] UniRef100_O31685 Bacillus subtilis YkvT1497 YkvU protein [Bacillus subtilis] UniRef100_O31686 Bacillus subtilisYkvU 1498 YkvV protein [Bacillus subtilis] UniRef100_O31687 Bacillussubtilis YkvV 1499 YkvW 1500 YkvY protein [Bacillus subtilis]UniRef100_O31689 Bacillus subtilis YkvY 1501 Necrosis and ethyleneinducing protein UniRef100_Q9KFT2 Bacillus halodurans [Bacillushalodurans] 1502 Putative HTH-type transcriptional UniRef100_O31690Bacillus subtilis YkvZ regulator ykvZ [Bacillus subtilis] 1503Transcription antiterminator [Bacillus UniRef100_O06710 Bacillussubtilis GlcT subtilis] 1504 PtsG 1505 Phosphocarrier protein HPr[Bacillus UniRef100_P08877 Bacillus subtilis subtilis] 1506Phosphoenolpyruvate-protein UniRef100_P08838 Bacillus subtilis PtsIphosphotransferase [Bacillus subtilis] 1507 SplA [Bacillusamyloliquefaciens] UniRef100_O54358 Bacillus amyloliquefaciens 1508Spore photoproduct lyase [Bacillus UniRef100_O54359 Bacillus SplBamyloliquefaciens] amyloliquefaciens 1509 Hypothetical protein orf1[Bacillus UniRef100_O05187 Bacillus subtilis YkwB subtilis] 1510Methyl-accepting chemotaxis protein UniRef100_P54576 Bacillus subtilisMcpC mcpC [Bacillus subtilis] 1511 3-oxoacyl-(Acyl carrier protein)UniRef100_Q8EMP9 Oceanobacillus FabG reductase [Oceanobacillusiheyensis] iheyensis 1512 Hypothetical oxidoreductase ykwCUniRef100_O34948 Bacillus subtilis YkwC [Bacillus subtilis] 1513 YkwDprotein [Bacillus subtilis] UniRef100_O31694 Bacillus subtilis YkwD 1514YkuA protein [Bacillus subtilis] UniRef100_O31399 Bacillus subtilis YkuA1515 Sporulation kinase A [Bacillus subtilis] UniRef100_P16497 Bacillussubtilis KinA 1516 Putative aminotransferase A [BacillusUniRef100_P16524 Bacillus subtilis PatA subtilis] 1517 1518 Hypotheticalprotein yxaI [Bacillus UniRef100_P42108 Bacillus subtilis YxaI subtilis]1519 YxiO 1520 Chemotaxis protein cheV [Bacillus UniRef100_P37599Bacillus subtilis CheV subtilis] 1521 Hypothetical protein ykyB[Bacillus UniRef100_P42430 Bacillus subtilis YkyB subtilis] 1522 YkuC1523 YkuD protein [Bacillus subtilis] UniRef100_O34816 Bacillus subtilisYkuD 1524 YkuE 1525 Hypothetical oxidoreductase ykuF UniRef100_O34717Bacillus subtilis YkuF [Bacillus subtilis] 1526 YkuI protein [Bacillussubtilis] UniRef100_O35014 Bacillus subtilis YkuI 1527 1528 YkuJ protein[Bacillus subtilis] UniRef100_O34588 Bacillus subtilis 1529 YkuK protein[Bacillus subtilis] UniRef100_O34776 Bacillus subtilis YkuK 1530Hypothetical protein ykzF [Bacillus UniRef100_O31697 Bacillus subtilissubtilis] 1531 YkuL protein [Bacillus subtilis] UniRef100_O31698Bacillus subtilis YkuL 1532 Putative HTH-type transcriptionalUniRef100_O34827 Bacillus subtilis CcpC regulator ykuM [Bacillussubtilis] 1533 Probable flavodoxin 1 [Bacillus subtilis]UniRef100_O34737 Bacillus subtilis YkuN 1534 YkuO protein [Bacillussubtilis] UniRef100_O34879 Bacillus subtilis YkuO 1535 Probableflavodoxin 2 [Bacillus subtilis] UniRef100_O34589 Bacillus subtilis YkuP1536 YkuQ protein [Bacillus subtilis] UniRef100_O34981 Bacillus subtilisYkuQ 1537 YkuR protein [Bacillus subtilis] UniRef100_O34916 Bacillussubtilis YkuR 1538 Hypothetical UPF0180 protein ykuS UniRef100_O34783Bacillus subtilis [Bacillus subtilis] 1539 YkuU protein [Bacillussubtilis] UniRef100_O34564 Bacillus subtilis YkuU 1540 YkuV protein[Bacillus subtilis] UniRef100_O31403 Bacillus subtilis YkuV 1541Repressor rok [Bacillus subtilis] UniRef100_O34857 Bacillus subtilis Rok1542 YknT protein [Bacillus subtilis] UniRef100_O31700 Bacillus subtilisYknT 1543 MobA 1544 Molybdopterin biosynthesis protein UniRef100_O31702Bacillus subtilis MoeB MoeB [Bacillus subtilis] 1545 Molybdopterinbiosynthesis protein UniRef100_O31703 Bacillus subtilis MoeA MoeA[Bacillus subtilis] 1546 Molybdopterin-guanine dinucleotideUniRef100_O31704 Bacillus subtilis MobB biosynthesis protein B [Bacillussubtilis] 1547 Molybdopterin converting factor subunit UniRef100_O31705Bacillus subtilis MoaE 2 [Bacillus subtilis] 1548 Molybdopterinconverting factor, UniRef100_O31706 Bacillus subtilis subunit 1[Bacillus subtilis] 1549 1550 YknU protein [Bacillus subtilis]UniRef100_O31707 Bacillus subtilis YknU 1551 YknV protein [Bacillussubtilis] UniRef100_O31708 Bacillus subtilis YknV 1552 Hypotheticalprotein yknW [Bacillus UniRef100_O31709 Bacillus subtilis YknW subtilis]1553 YknX protein [Bacillus subtilis] UniRef100_O31710 Bacillus subtilisYknX 1554 YknY protein [Bacillus subtilis] UniRef100_O31711 Bacillussubtilis YknY 1555 Hypothetical protein yknZ [Bacillus UniRef100_O31712Bacillus subtilis YknZ subtilis] 1556 FruR 1557 1-phosphofructokinase[Bacillus UniRef100_O31714 Bacillus subtilis FruK subtilis] 1558Phosphotransferase system (PTS) UniRef100_P71012 Bacillus subtilis FruAfructose-specific enzyme IIABC component [Bacillus subtilis] 1559 Signalpeptidase I T [Bacillus subtilis] UniRef100_P71013 Bacillus subtilisSipT 1560 Hypothetical protein ykoA [Bacillus UniRef100_O31715 Bacillussubtilis subtilis] 1561 1562 YkpA protein [Bacillus subtilis]UniRef100_O31716 Bacillus subtilis YkpA 1563 BH1921 protein [Bacillushalodurans] UniRef100_Q9KBK5 Bacillus halodurans 1564 AminopeptidaseampS [Bacillus subtilis] UniRef100_P39762 Bacillus subtilis AmpS 15651566 MreBH protein [Bacillus subtilis] UniRef100_P39763 Bacillussubtilis MreBH 1567 1568 Sporulation kinase C [Bacillus subtilis]UniRef100_P39764 Bacillus subtilis KinC 1569 Hypothetical protein ykqB[Bacillus UniRef100_P39760 Bacillus subtilis YkqB subtilis] 1570 Adeninedeaminase [Bacillus subtilis] UniRef100_P39761 Bacillus subtilis AdeC1571 YkqC 1572 YkzG protein [Bacillus subtilis] UniRef100_O31718Bacillus subtilis 1573 Hypothetical protein ykrA [BacillusUniRef100_Q45494 Bacillus subtilis YkrA subtilis] 1574 YkrB 1575 1576Hypothetical protein ykyA [Bacillus UniRef100_P21884 Bacillus subtilisYkyA subtilis] 1577 Pyruvate dehydrogenase E1 UniRef100_P21881 Bacillussubtilis PdhA component, alpha subunit [Bacillus subtilis] 1578 Pyruvatedehydrogenase E1 UniRef100_P21882 Bacillus subtilis PdhB component, betasubunit [Bacillus subtilis] 1579 Dihydrolipoyllysine-residueUniRef100_P21883 Bacillus subtilis PdhC acetyltransferase component ofpyruvate dehydrogenase complex [Bacillus subtilis] 1580 Dihydrolipoyldehydrogenase [Bacillus UniRef100_P21880 Bacillus subtilis PdhDsubtilis] 1581 UPI00003CC069 UniRef100 entry UniRef100_UPI00003CC0691582 IS1627s1-related, transposase [Bacillus UniRef100_Q7CMD0 Bacillusanthracis str. A2012 anthracis str. A2012] 1583 1584 1585 Argininedecarboxylase [Bacillus UniRef100_P21885 Bacillus subtilis SpeAsubtilis] 1586 Hypothetical UPF0223 protein yktA UniRef100_Q45497Bacillus subtilis [Bacillus subtilis] 1587 Hypothetical protein yktB[Bacillus UniRef100_Q45498 Bacillus subtilis YktB subtilis] 1588 YkzIprotein [Bacillus subtilis] UniRef100_O31719 Bacillus subtilis 1589Inositol-1-monophosphatase [Bacillus UniRef100_Q45499 Bacillus subtilisYktC subtilis] 1590 Hypothetical protein ykzC [Bacillus UniRef100_O31720Bacillus subtilis YkzC subtilis] 1591 Hypothetical protein ylaA[Bacillus UniRef100_O07625 Bacillus subtilis YlaA subtilis] 1592Hypothetical protein ylaB [Bacillus UniRef100_O07626 Bacillus subtilissubtilis] 1593 YlaC protein [Bacillus subtilis] UniRef100_O07627Bacillus subtilis YlaC 1594 Hypothetical protein ylaD [BacillusUniRef100_O07628 Bacillus subtilis subtilis] 1595 Hypothetical proteinylaF [Bacillus UniRef100_O07630 Bacillus subtilis subtilis] 1596GTP-binding protein typA/bipA homolog UniRef100_O07631 Bacillus subtilisYlaG [Bacillus subtilis] 1597 YlaH protein [Bacillus subtilis]UniRef100_O07632 Bacillus subtilis YlaH 1598 YhzA homolog [Bacillussubtilis] UniRef100_O07562 Bacillus subtilis YhjH 1599 Hypotheticalprotein yhjG [Bacillus UniRef100_O07561 Bacillus subtilis YhjG subtilis]1600 1601 Hypothetical lipoprotein ylaJ precursor UniRef100_O07634Bacillus subtilis YlaJ [Bacillus subtilis] 1602 YlaK protein [Bacillussubtilis] UniRef100_O07635 Bacillus subtilis YlaK 1603 UPI00003CB7B1UniRef100 entry UniRef100_UPI00003CB7B1 YlaL 1604 Probable glutaminaseylaM [Bacillus UniRef100_O07637 Bacillus subtilis YlaM subtilis] 1605YlaN protein [Bacillus subtilis] UniRef100_O07638 Bacillus subtilis 1606Hypothetical protein ylaO [Bacillus UniRef100_O07639 Bacillus subtilisFtsW subtilis] 1607 PycA 1608 Cytochrome AA3 controlling proteinUniRef100_P12946 Bacillus subtilis CtaA [Bacillus subtilis] 1609Protoheme IX farnesyltransferase UniRef100_P24009 Bacillus subtilis CtaB[Bacillus subtilis] 1610 Cytochrome c oxidase polypeptide IIUniRef100_P24011 Bacillus subtilis CtaC precursor (EC 1.9.3.1)(Cytochrome aa3 subunit 2) (Caa-3605 subunit 2) (Oxidase aa(3) subunit2) [Bacillus subtilis] 1611 Cytochrome c oxidase polypeptide IUniRef100_P24010 Bacillus subtilis CtaD (EC 1.9.3.1) (Cytochrome aa3subunit 1) (Caa-3605 subunit 1) (Oxidase aa(3) subunit 1) [Bacillussubtilis] 1612 Cytochrome c oxidase polypeptide III UniRef100_P24012Bacillus subtilis CtaE (EC 1.9.3.1) (Cytochrome aa3 subunit 3) (Caa-3605subunit 3) (Oxidase aa(3) subunit 3) [Bacillus subtilis] 1613 Cytochromec oxidase polypeptide IVB UniRef100_P24013 Bacillus subtilis CtaF[Bacillus subtilis] 1614 CtaG protein [Bacillus subtilis]UniRef100_O34329 Bacillus subtilis CtaG 1615 YlbA protein [Bacillussubtilis] UniRef100_O34743 Bacillus subtilis YlbA 1616 YlbB protein[Bacillus subtilis] UniRef100_O34682 Bacillus subtilis YlbB 1617 YlbCprotein [Bacillus subtilis] UniRef100_O34586 Bacillus subtilis YlbC 1618YlbD protein [Bacillus subtilis] UniRef100_O34880 Bacillus subtilis YlbD1619 YlbE protein [Bacillus subtilis] UniRef100_O34958 Bacillus subtilis1620 Regulatory protein ylbF [Bacillus UniRef100_O34412 Bacillussubtilis YlbF subtilis] 1621 Hypothetical UPF0298 protein ylbGUniRef100_O34658 Bacillus subtilis [Bacillus subtilis] 1622 YlbH protein[Bacillus subtilis] UniRef100_O34331 Bacillus subtilis YlbH 1623Phosphopantetheine UniRef100_O34797 Bacillus subtilis YlbIadenylyltransferase [Bacillus subtilis] 1624 YlbJ 1625 YlbL protein[Bacillus subtilis] UniRef100_O34470 Bacillus subtilis YlbL 1626 YlbMprotein [Bacillus subtilis] UniRef100_O34513 Bacillus subtilis YlbM 1627YlbN protein [Bacillus subtilis] UniRef100_O34445 Bacillus subtilis YlbN1628 50S ribosomal protein L32 [Bacillus UniRef100_O34687 Bacillussubtilis subtilis] 1629 Hypothetical protein ylbO [BacillusUniRef100_O34549 Bacillus subtilis YlbO subtilis] 1630 YlbP protein[Bacillus subtilis] UniRef100_O34468 Bacillus subtilis YlbP 1631Probable 2-dehydropantoate 2- UniRef100_O34661 Bacillus subtilis YlbQreductase [Bacillus subtilis] 1632 YllA 1633 Protein mraZ [Bacillussubtilis] UniRef100_P55343 Bacillus subtilis YllB 1634S-adenosyl-methyltransferase mraW UniRef100_Q07876 Bacillus subtilisYlxA [Bacillus subtilis] 1635 FtsL 1636 Penicillin-binding protein 2B[Bacillus UniRef100_Q07868 Bacillus subtilis PbpB subtilis] 1637 Stage Vsporulation protein D [Bacillus UniRef100_Q03524 Bacillus subtilis SpoVDsubtilis] 1638 UDP-N-acetylmuramoylalanyl-D- UniRef100_Q03523 Bacillussubtilis MurE glutamate--2,6-diaminopimelate ligase [Bacillus subtilis]1639 Phospho-N-acetylmuramoyl- UniRef100_Q03521 Bacillus subtilis MraYpentapeptide-transferase [Bacillus subtilis] 1640UDP-N-acetylmuramoylalanine--D- UniRef100_Q03522 Bacillus subtilis MurDglutamate ligase [Bacillus subtilis] 1641 Stage V sporulation protein E[Bacillus UniRef100_P07373 Bacillus subtilis SpoVE subtilis] 1642UDP-N-acetylglucosamine--N- UniRef100_P37585 Bacillus subtilis MurGacetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [Bacillus subtilis] 1643UDP-N-acetylenolpyruvoylglucosamine UniRef100_P18579 Bacillus subtilisMurB reductase [Bacillus subtilis] 1644 DivIB 1645 YlxW 1646 YlxX 1647Sbp 1648 FtsA 1649 Cell division protein ftsZ [Bacillus UniRef100_P17865Bacillus subtilis FtsZ subtilis] 1650 Bpr 1651 Bacillopeptidase Fprecursor [Bacillus UniRef100_P16397 Bacillus subtilis Bpr subtilis]1652 Sporulation sigma-E factor processing UniRef100_P13801 Bacillussubtilis SpoIIGA peptidase [Bacillus subtilis] 1653 RNA polymerasesigma-E factor UniRef100_P06222 Bacillus subtilis SigE precursor[Bacillus subtilis] 1654 RNA polymerase sigma-G factor UniRef100_P19940Bacillus subtilis SigG [Bacillus subtilis] 1655 YlmA protein [Bacillussubtilis] UniRef100_O31723 Bacillus subtilis YlmA 1656 1657 YlmC protein[Bacillus subtilis] UniRef100_O31725 Bacillus subtilis 1658 HypotheticalUPF0124 protein ylmD UniRef100_O31726 Bacillus subtilis YlmD [Bacillussubtilis] 1659 YlmE 1660 YlmF protein [Bacillus subtilis]UniRef100_O31728 Bacillus subtilis YlmF 1661 YlmG protein [Bacillussubtilis] UniRef100_O31729 Bacillus subtilis 1662 Minicell-associatedprotein [Bacillus UniRef100_P71020 Bacillus subtilis YlmH subtilis] 1663Minicell-associated protein DivIVA UniRef100_P71021 Bacillus subtilisDivIVA [Bacillus subtilis] 1664 Isoleucyl-tRNA synthetase [BacillusUniRef100_Q45477 Bacillus subtilis IleS subtilis] 1665 YlyA 1666 LspA1667 Hypothetical pseudouridine synthase UniRef100_Q45480 Bacillussubtilis YlyB ylyB [Bacillus subtilis] 1668 PyrR bifunctional protein[Includes: UniRef100_P39765 Includes: PyrR Pyrimidine operon regulatoryprotein; Pyrimidine Uracil phosphoribosyltransferase (EC operon 2.4.2.9)(UPRTase)] [Bacillus subtilis] regulatory protein; Uracilphosphoribosyltransferase (EC 2.4.2.9) (UPRTase) 1669 Uracil permease[Bacillus subtilis] UniRef100_P39766 Bacillus subtilis PyrP 1670Aspartate carbamoyltransferase UniRef100_P05654 Bacillus subtilis PyrB[Bacillus subtilis] 1671 Dihydroorotase [Bacillus subtilis]UniRef100_P25995 Bacillus subtilis PyrC 1672 Carbamoyl-phosphatesynthase, UniRef100_P25993 Bacillus subtilis PyrAA pyrimidine-specific,small chain [Bacillus subtilis] 1673 Carbamoyl-phosphate synthase,UniRef100_P25994 Bacillus subtilis PyrAB pyrimidine-specific, largechain [Bacillus subtilis] 1674 Dihydroorotate dehydrogenase electronUniRef100_P25983 Bacillus subtilis PyrK transfer subunit [Bacillussubtilis] 1675 Dihydroorotate dehydrogenase, UniRef100_P25996 Bacillussubtilis PyrD catalytic subunit [Bacillus subtilis] 1676 Orotidine5′-phosphate decarboxylase UniRef100_P25971 Bacillus subtilis PyrF[Bacillus subtilis] 1677 Orotate phosphoribosyltransferaseUniRef100_P25972 Bacillus subtilis PyrE [Bacillus subtilis] 1678 1679CysH 1680 YlnA protein [Bacillus subtilis] UniRef100_O34734 Bacillussubtilis CysP 1681 Sulfate adenylyltransferase [BacillusUniRef100_O34764 Bacillus subtilis Sat subtilis] 1682 Probableadenylyl-sulfate kinase UniRef100_O34577 Bacillus subtilis CysC[Bacillus subtilis] 1683 Putative S-adenosyl L-methionine:UniRef100_O34744 Bacillus subtilis YlnD uroporphyrinogen IIImethyltransferase [Bacillus subtilis] 1684 Sirohydrochlorinferrochelatase UniRef100_O34632 Bacillus subtilis YlnE [Bacillussubtilis] 1685 YlnF protein [Bacillus subtilis] UniRef100_O34813Bacillus subtilis YlnF 1686 Putative fibronectin-binding proteinUniRef100_O34693 Bacillus subtilis YloA [Bacillus subtilis] 1687 YloBprotein [Bacillus subtilis] UniRef100_O34431 Bacillus subtilis YloB 1688YloC protein [Bacillus subtilis] UniRef100_O34441 Bacillus subtilis YloC1689 Hypothetical UPF0296 protein ylzA UniRef100_Q7WY72 Bacillussubtilis [Bacillus subtilis] 1690 Guanylate kinase [Bacillus subtilis]UniRef100_O34328 Bacillus subtilis Gmk 1691 DNA-directed RNA polymeraseomega UniRef100_O35011 Bacillus subtilis chain [Bacillus subtilis] 1692YloI protein [Bacillus subtilis] UniRef100_O35033 Bacillus subtilis YloI1693 Primosomal protein N′ [Bacillus subtilis] UniRef100_P94461 Bacillussubtilis PriA 1694 Peptide deformylase 1 [Bacillus subtilis]UniRef100_P94462 Bacillus subtilis Def 1695 Methionyl-tRNAformyltransferase UniRef100_P94463 Bacillus subtilis Fmt [Bacillussubtilis] 1696 Ribosomal RNA small subunit UniRef100_P94464 Bacillussubtilis YloM methyltransferase B (EC 2.1.1.—) (rRNA(cytosine-C(5)-)-methyltransferase) [Bacillus subtilis] 1697Hypothetical UPF0063 protein yloN UniRef100_O34617 Bacillus subtilisYloN [Bacillus subtilis] 1698 Protein phosphatase [Bacillus subtilis]UniRef100_O34779 Bacillus subtilis PrpC 1699 Probableserine/threonine-protein UniRef100_O34507 Bacillus subtilis PrkC kinaseyloP [Bacillus subtilis] 1700 Probable GTPase engC [BacillusUniRef100_O34530 Bacillus subtilis YloQ subtilis] 1701Ribulose-phosphate 3-epimerase UniRef100_O34557 Bacillus subtilis Rpe[Bacillus subtilis] 1702 YloS protein [Bacillus subtilis]UniRef100_O34664 Bacillus subtilis YloS 1703 1704 50S ribosomal proteinL28 [Bacillus UniRef100_P37807 Bacillus subtilis subtilis] 1705Hypothetical protein yloU [Bacillus UniRef100_O34318 Bacillus subtilisYloU subtilis] 1706 YloV 1707 Probable L-serine dehydratase, betaUniRef100_O34635 Bacillus subtilis SdaAB chain [Bacillus subtilis] 1708Probable L-serine dehydratase, alpha UniRef100_O34607 Bacillus subtilisSdaAA chain [Bacillus subtilis] 1709 ATP-dependent DNA helicase recGUniRef100_O34942 Bacillus subtilis RecG [Bacillus subtilis] 1710Transcription factor fapR [Bacillus UniRef100_O34835 Bacillus subtilisYlpC subtilis] 1711 Fatty acid/phospholipid synthesis UniRef100_P71018Bacillus subtilis PlsX protein plsX [Bacillus subtilis] 1712 MalonylCoA-acyl carrier protein UniRef100_P71019 Bacillus subtilis FabDtransacylase [Bacillus subtilis] 1713 3-oxoacyl-[acyl-carrier-protein]UniRef100_P51831 acyl-carrier- FabG reductase [Bacillus subtilis]protein 1714 Acyl carrier protein [Bacillus subtilis] UniRef100_P80643Bacillus subtilis 1715 Rnc 1716 Chromosome partition protein smcUniRef100_P51834 Bacillus subtilis Smc [Bacillus subtilis] 1717 FtsY1718 1719 Signal recognition particle protein UniRef100_P37105 Bacillussubtilis Ffh [Bacillus subtilis] 1720 30S ribosomal protein S16[Bacillus UniRef100_P21474 Bacillus subtilis subtilis] 1721 1722 YlqDprotein [Bacillus subtilis] UniRef100_O31739 Bacillus subtilis YlqD 1723RimM 1724 TrmD 1725 50S ribosomal protein L19 [Bacillus UniRef100_O31742Bacillus subtilis RplS subtilis] 1726 YlqF protein [Bacillus subtilis]UniRef100_O31743 Bacillus subtilis YlqF 1727 Ribonuclease HII [Bacillussubtilis] UniRef100_O31744 Bacillus subtilis RnhB 1728 YlqG protein[Bacillus subtilis] UniRef100_O31745 Bacillus subtilis YlqG 1729 YlqHprotein [Bacillus subtilis] UniRef100_O34867 Bacillus subtilis 1730Succinyl-CoA synthetase beta chain UniRef100_P80886 Bacillus subtilisSucC [Bacillus subtilis] 1731 Succinyl-CoA synthetase alpha chainUniRef100_P80865 Bacillus subtilis SucD [Bacillus subtilis] 1732 Smf1733 TopA 1734 Protein gid [Bacillus subtilis] UniRef100_P39815 Bacillussubtilis Gid 1735 Tyrosine recombinase xerC [Bacillus UniRef100_P39776Bacillus subtilis CodV subtilis] 1736 ATP-dependent protease hslVUniRef100_P39070 Bacillus subtilis ClpQ precursor [Bacillus subtilis]1737 ATP-dependent hsl protease ATP- UniRef100_P39778 Bacillus subtilisClpY binding subunit hslU [Bacillus subtilis] 1738 GTP-sensingtranscriptional pleiotropic UniRef100_P39779 Bacillus subtilis CodYrepressor codY [Bacillus subtilis] 1739 Flagellar basal-body rod proteinflgB UniRef100_P24500 Bacillus subtilis FlgB [Bacillus subtilis] 1740Flagellar basal-body rod protein flgC UniRef100_P24501 Bacillus subtilisFlgC [Bacillus subtilis] 1741 Flagellar hook-basal body complexUniRef100_P24502 Bacillus subtilis FliE protein fliE [Bacillus subtilis]1742 Flagellar M-ring protein [Bacillus UniRef100_P23447 Bacillussubtilis FliF subtilis] 1743 Flagellar motor switch protein fliGUniRef100_P23448 Bacillus subtilis FliG [Bacillus subtilis] 1744Probable flagellar assembly protein fliH UniRef100_P23449 Bacillussubtilis FliH [Bacillus subtilis] 1745 Flagellum-specific ATP synthaseUniRef100_P23445 Bacillus subtilis FliI [Bacillus subtilis] 1746Flagellar fliJ protein [Bacillus subtilis] UniRef100_P20487 Bacillussubtilis FliJ 1747 FlaA locus 22.9 kDa protein [BacillusUniRef100_P23454 Bacillus subtilis YlxF subtilis] 1748 Probableflagellar hook-length control UniRef100_P23451 Bacillus subtilis FliKprotein [Bacillus subtilis] 1749 FlaA locus hypothetical protein ylxGUniRef100_P23455 Bacillus subtilis YlxG [Bacillus subtilis] 1750 FlgE1751 BH2448 protein [Bacillus halodurans] UniRef100_Q9KA42 Bacillushalodurans 1752 Flagellar fliL protein [Bacillus subtilis]UniRef100_P23452 Bacillus subtilis FliL 1753 Flagellar motor switchprotein fliM UniRef100_P23453 Bacillus subtilis FliM [Bacillus subtilis]1754 Flagellar motor switch protein fliY UniRef100_P24073 Bacillussubtilis FliY [Bacillus subtilis] 1755 Chemotaxis protein cheY homologUniRef100_P24072 Bacillus subtilis CheY [Bacillus subtilis] 1756Flagellar biosynthetic protein fliZ UniRef100_P35536 Bacillus subtilisFliZ precursor [Bacillus subtilis] 1757 Flagellar biosynthetic proteinfliP UniRef100_P35528 Bacillus subtilis FliP [Bacillus subtilis] 1758Flagellar biosynthetic protein fliQ UniRef100_P35535 Bacillus subtilis[Bacillus subtilis] 1759 Flagellar biosynthetic protein fliRUniRef100_P35537 Bacillus subtilis FliR [Bacillus subtilis] 1760Flagellar biosynthetic protein flhB UniRef100_P35538 Bacillus subtilisFlhB [Bacillus subtilis] 1761 Flagellar biosynthesis protein flhAUniRef100_P35620 Bacillus subtilis FlhA [Bacillus subtilis] 1762Flagellar biosynthesis protein flhF UniRef100_Q01960 Bacillus subtilisFlhF [Bacillus subtilis] 1763 Hypothetical protein ylxH [BacillusUniRef100_P40742 Bacillus subtilis YlxH subtilis] 1764 Chemotaxisresponse regulator protein- UniRef100_Q05522 Bacillus subtilis CheBglutamate methylesterase [Bacillus subtilis] 1765 Chemotaxis proteincheA [Bacillus UniRef100_P29072 Bacillus subtilis CheA subtilis] 1766Chemotaxis protein cheW [Bacillus UniRef100_P39802 Bacillus subtilisCheW subtilis] 1767 Chemotaxis protein cheC [Bacillus UniRef100_P40403Bacillus subtilis CheC subtilis] 1768 Chemotaxis protein cheD [BacillusUniRef100_P40404 Bacillus subtilis CheD subtilis] 1769 RNA polymerasesigma-D factor UniRef100_P10726 Bacillus subtilis SigD [Bacillussubtilis] 1770 Swarming motility protein swrB [Bacillus UniRef100_P40405Bacillus subtilis YlxL subtilis] 1771 30S ribosomal protein S2 [BacillusUniRef100_P21464 Bacillus subtilis RpsB subtilis] 1772 Translationelongation factor Ts UniRef100_Q65JJ8 Bacillus cereus Tsf [Bacilluscereus] 1773 PyrH 1774 Frr 1775 Undecaprenyl pyrophosphateUniRef100_O31751 Bacillus subtilis UppS synthetase [Bacillus subtilis]1776 Phosphatidate cytidylyltransferase UniRef100_O31752 Bacillussubtilis CdsA [Bacillus subtilis] 1777 1-deoxy-D-xylulose 5-phosphateUniRef100_O31753 Bacillus subtilis Dxr reductoisomerase [Bacillussubtilis] 1778 Hypothetical zinc metalloprotease yluc UniRef100_O31754Bacillus subtilis YluC [Bacillus subtilis] 1779 Prolyl-tRNA synthetase[Bacillus UniRef100_O31755 Bacillus subtilis ProS subtilis] 1780 DNApolymerase III polC-type [Bacillus UniRef100_P13267 Bacillus subtilisPolC subtilis] 1781 1782 Cellulose 1,4-beta-cellobiosidaseUniRef100_Q8KKF7 Paenibacillus sp. BP-23 precursor [Paenibacillus sp.BP-23] 1783 Endoglucanase B precursor UniRef100_P23550 Paenibacilluslautus [Paenibacillus lautus] 1784 Beta-mannosidase [ThermotogaUniRef100_Q9RIK7 Thermotoga neapolitana neapolitana] 1785 HypotheticalUPF0090 protein ylxS UniRef100_P32726 Bacillus subtilis YlxS [Bacillussubtilis] 1786 Transcription elongation protein nusA UniRef100_P32727Bacillus subtilis NusA [Bacillus subtilis] 1787 Hypothetical proteinylxR [Bacillus UniRef100_P32728 Bacillus subtilis subtilis] 1788Probable ribosomal protein ylxQ UniRef100_P32729 Bacillus subtilis[Bacillus subtilis] 1789 Translation initiation factor IF-2UniRef100_P17889 Bacillus subtilis InfB [Bacillus subtilis] 1790Hypothetical protein ylxP [Bacillus UniRef100_P32730 Bacillus subtilissubtilis] 1791 Ribosome-binding factor A [Bacillus UniRef100_P32731Bacillus subtilis RbfA subtilis] 1792 TruB 1793 Riboflavin biosynthesisprotein ribC UniRef100_P54575 Includes: RibC [Includes: Riboflavinkinase (EC Riboflavin kinase 2.7.1.26) (Flavokinase); FMN (EC 2.7.1.26)adenylyltransferase (EC 2.7.7.2) (FAD (Flavokinase); pyrophosphorylase)(FAD synthetase)] FMN [Bacillus subtilis] adenylyltransferase (EC2.7.7.2) (FAD pyrophosphorylase) (FAD synthetase) 1794 30S ribosomalprotein S15 [Bacillus UniRef100_P21473 Bacillus subtilis subtilis] 1795Polyribonucleotide UniRef100_P50849 Bacillus subtilis PnpAnucleotidyltransferase [Bacillus subtilis] 1796 Hypothetical proteinylxY precursor UniRef100_P50850 Bacillus subtilis YlxY [Bacillussubtilis] 1797 Hypothetical zinc protease ymxG UniRef100_Q04805 Bacillussubtilis MlpA [Bacillus subtilis] 1798 Hypothetical protein ymxH[Bacillus UniRef100_Q04811 Bacillus subtilis subtilis] 1799 Dipicolinatesynthase, A chain [Bacillus UniRef100_Q04809 Bacillus subtilis SpoVFAsubtilis] 1800 Dipicolinate synthase, B chain [Bacillus UniRef100_Q04810Bacillus subtilis SpoVFB subtilis] 1801 Aspartate-semialdehydeUniRef100_Q04797 Bacillus subtilis Asd dehydrogenase [Bacillus subtilis]1802 Aspartokinase 1 (EC 2.7.2.4) UniRef100_Q04795 Contains: DapG(Aspartokinase I) (Aspartate kinase 1) Aspartokinase I [Contains:Aspartokinase I alpha alpha subunit; subunit; Aspartokinase I betasubunit] Aspartokinase I [Bacillus subtilis] beta subunit 1803Dihydrodipicolinate synthase [Bacillus UniRef100_Q04796 Bacillussubtilis DapA subtilis] 1804 YmfA protein [Bacillus subtilis]UniRef100_O31760 Bacillus subtilis YmfA 1805 Translocation-enhancingprotein tepA UniRef100_Q99171 Bacillus subtilis TepA [Bacillus subtilis]1806 1807 DNA translocase ftsK [Bacillus subtilis] UniRef100_P21458Bacillus subtilis SpoIIIE 1808 Hypothetical transcriptional regulatorUniRef100_O31761 Bacillus subtilis YmfC ymfC [Bacillus subtilis] 1809Multidrug resistance protein [Bacillus UniRef100_Q9K7Q2 Bacillus YitGhalodurans] halodurans 1810 Hypothetical protein [Bacillus anthracis]UniRef100_Q81WP6 Bacillus YmfG anthracis 1811 YmfH protein [Bacillussubtilis] UniRef100_O31766 Bacillus subtilis YmfH 1812 1813 1814 YmfJprotein [Bacillus subtilis] UniRef100_O31768 Bacillus subtilis 1815Hypothetical protein [Bacillus cereus UniRef100_Q636P2 Bacillus cereusYmfK ZK] ZK 1816 YmfM 1817 CDP-diacylglycerol--glycerol-3-UniRef100_P46322 Bacillus subtilis PgsA phosphate3-phosphatidyltransferase [Bacillus subtilis] 1818 CinA-like protein[Bacillus subtilis] UniRef100_P46323 Bacillus subtilis CinA 1819 RecAprotein [Bacillus UniRef100_Q8GJG2 Bacillus RecA amyloliquefaciens]amyloliquefaciens 1820 Hypothetical UPF0144 protein ymdAUniRef100_O31774 Bacillus subtilis YmdA [Bacillus subtilis] 1821 YmdBprotein [Bacillus subtilis] UniRef100_O31775 Bacillus subtilis YmdB 1822Stage V sporulation protein S [Bacillus UniRef100_P45693 Bacillussubtilis subtilis] 1823 1824 1825 L-threonine 3-dehydrogenase [BacillusUniRef100_O31776 Bacillus subtilis Tdh subtilis] 18262-amino-3-ketobutyrate coenzyme A UniRef100_O31777 Bacillus subtilis Kblligase [Bacillus subtilis] 1827 Hypothetical UPF0004 protein ymcBUniRef100_O31778 Bacillus subtilis YmcB [Bacillus subtilis] 1828 YmcAprotein [Bacillus subtilis] UniRef100_O31779 Bacillus subtilis YmcA 1829Spore coat protein E [Bacillus subtilis] UniRef100_P14016 Bacillussubtilis CotE 1830 MutS 1831 DNA mismatch repair protein mutLUniRef100_P49850 Bacillus subtilis MutL [Bacillus subtilis] 1832 YjcSprotein [Bacillus subtilis] UniRef100_O31641 Bacillus subtilis 1833 YxiD1834 1835 1836 AII1751 protein [Anabaena sp.] UniRef100_Q8YW65 Anabaenasp. YciC 1837 BH0367 protein [Bacillus halodurans] UniRef100_Q9KFV4Bacillus halodurans 1838 Phosphinothricin N-acetyltransferaseUniRef100_Q9KFP5 Bacillus YwnH [Bacillus halodurans] halodurans 1839UPI00003CC0D8 UniRef100 entry UniRef100_UPI00003CC0D8 1840 1841 PutativeL-amino acid oxidase [Bacillus UniRef100_O34363 Bacillus subtilis YobNsubtilis] 1842 YoaK 1843 Na+/H+ antiporter [Bacillus halodurans]UniRef100_Q9K5Q0 Bacillus YvgP halodurans 1844 Aromatic hydrocarboncatabolism UniRef100_Q8CV32 Oceanobacillus iheyensis protein[Oceanobacillus iheyensis] 1845 Hypothetical UPF0145 protein VP1283UniRef100_Q87Q67 Vibrio parahaemolyticus [Vibrio parahaemolyticus] 1846Hypothetical protein yqeD [Bacillus UniRef100_P54449 Bacillus subtilisYqeD subtilis] 1847 Penicillin-binding protein, putativeUniRef100_Q738U9 Bacillus cereus PbpE [Bacillus cereus] 1848Hypothetical glycosyl transferase UniRef100_O34539 Bacillus subtilisYjiC [Bacillus subtilis] 1849 Asparate-proton symporter [BacillusUniRef100_O07002 Bacillus subtilis YveA subtilis] 1850 Spore coatprotein [Bacillus halodurans] UniRef100_Q9KEV6 Bacillus halodurans 18511852 YdhD 1853 Putative HTH-type transcriptional UniRef100_Q7WY76Bacillus subtilis YezE regulator yezE [Bacillus subtilis] 1854Hypothetical protein yesE [Bacillus UniRef100_O31511 Bacillus subtilisYesE subtilis] 1855 YesF protein [Bacillus subtilis] UniRef100_O31512Bacillus subtilis YesF 1856 UPI00003CBA3B UniRef100 entryUniRef100_UPI00003CBA3B 1857 YmaD protein [Bacillus subtilis]UniRef100_O31790 Bacillus subtilis YmaD 1858 Multidrug resistanceprotein ebrB UniRef100_O31791 Bacillus subtilis EbrB [Bacillus subtilis]1859 Multidrug resistance protein ebrA UniRef100_O31792 Bacillussubtilis EbrA [Bacillus subtilis] 1860 1861 Hypothetical protein ymaF[Bacillus UniRef100_O31794 Bacillus subtilis YmaF subtilis] 1862 tRNAdelta(2)- UniRef100_O31795 Bacillus subtilis MiaAisopentenylpyrophosphate transferase [Bacillus subtilis] 1863 Hfqprotein [Bacillus subtilis] UniRef100_O31796 Bacillus subtilis 1864Hypothetical protein ymzA [Bacillus UniRef100_O31798 Bacillus subtilissubtilis] 1865 NrdI protein [Bacillus subtilis] UniRef100_P50618Bacillus subtilis YmaA 1866 Ribonucleoside-diphosphate reductaseUniRef100_P50620 Bacillus subtilis NrdE alpha chain [Bacillus subtilis]1867 Ribonucleoside-diphosphate reductase UniRef100_P50621 Bacillussubtilis NrdF beta chain [Bacillus subtilis] 1868 Hypothetical proteinymaB [Bacillus UniRef100_P50619 Bacillus subtilis YmaB subtilis] 1869Blr6966 protein [Bradyrhizobium UniRef100_Q89EV4 Bradyrhizobium YtnPjaponicum] japonicum 1870 Nitrogen fixation protein [BacillusUniRef100_Q9KFV2 Bacillus YurV halodurans] halodurans 1871 Transcriptionregulator Fur family-like UniRef100_Q8CNQ7 Staphylococcus PerR protein[Staphylococcus epidermidis] epidermidis 1872 UPI00003CB681 UniRef100entry UniRef100_UPI00003CB681 YdhC 1873 Hypothetical protein [Bacilluscereus] UniRef100_Q81C60 Bacillus cereus YjlA 1874 CwlC 1875 Membraneprotein, putative [Listeria UniRef100_Q720L9 Listeria monocytogenesmonocytogenes] 1876 Lin1174 protein [Listeria innocua] UniRef100_Q92CJ7Listeria innocua 1877 1878 1879 Transcriptional regulator [AquifexUniRef100_O66635 Aquifex aeolicus YdgC aeolicus] 1880 Hypotheticalmembrane-anchored UniRef100_Q92VA1 Rhizobium meliloti protein [Rhizobiummeliloti] 1881 Cytosine permease [Bacillus UniRef100_Q9KBP3 BacillusYxlA halodurans] halodurans 1882 AgaF [Agrobacterium tumefaciens]UniRef100_O50265 Agrobacterium tumefaciens 1883 1884 Hydantoinutilization protein B UniRef100_Q88H51 Pseudomonas putida [Pseudomonasputida] 1885 Hypothetical protein SMb20139 UniRef100_Q92X23 Rhizobiummeliloti [Rhizobium meliloti] 1886 BH2340 protein [Bacillus halodurans]UniRef100_Q9KAE7 Bacillus halodurans 1887 1888 1889 Stage V sporulationprotein K [Bacillus UniRef100_P27643 Bacillus subtilis SpoVK subtilis]1890 YnbA [Bacillus subtilis] UniRef100_P94478 Bacillus subtilis YnbA1891 YnbB [Bacillus subtilis] UniRef100_P94479 Bacillus subtilis YnbB1892 HTH-type transcriptional regulator glnR UniRef100_P37582 Bacillussubtilis GlnR [Bacillus subtilis] 1893 Glutamine synthetase [Bacillussubtilis] UniRef100_P12425 Bacillus subtilis GlnA 1894 1895 Hypotheticalprotein CAC3435 UniRef100_Q97DN7 Clostridium acetobutylicum [Clostridiumacetobutylicum] 1896 1897 1898 Hypothetical protein CAC0350UniRef100_Q97M50 Clostridium acetobutylicum [Clostridium acetobutylicum]1899 Hypothetical HIT-like protein MJ0866 UniRef100_Q58276 MethanococcusHit [Methanococcus jannaschii] jannaschii 1900 Hypothetical protein[Bacillus anthracis] UniRef100_Q6I2B3 Bacillus anthracis 1901Hypothetical protein [Bacillus cereus] UniRef100_Q81CM1 Bacillus cereusYdjC 1902 1903 Methyltransferase [Bacillus cereus] UniRef100_Q81CJ2Bacillus cereus 1904 YoaO 1905 Acetyltransferase, GNAT familyUniRef100_Q737B4 Bacillus cereus [Bacillus cereus] 1906 Repressor rok[Bacillus subtilis] UniRef100_O34857 Bacillus subtilis Rok 1907 1908Hypothetical protein [Bacteriophage T5] UniRef100_Q6QGK2 BacteriophageT5 1909 Hypothetical protein yvdT [Bacillus UniRef100_O07001 Bacillussubtilis YvdT subtilis] 1910 Hypothetical protein yvdS [BacillusUniRef100_O32262 Bacillus subtilis YvdS subtilis] 1911 Hypotheticalprotein yvdR [Bacillus UniRef100_O06999 Bacillus subtilis YvdR subtilis]1912 Spermidine N1-acetyltransferase UniRef100_Q72Y03 Bacillus cereusYoaA [Bacillus cereus] 1913 Hypothetical Membrane AssociatedUniRef100_Q812L6 Bacillus cereus Protein [Bacillus cereus] 1914Hypothetical protein yoaW precursor UniRef100_O34541 Bacillus subtilisYoaW [Bacillus subtilis] 1915 Thiol-disulfide oxidoreductase resAUniRef100_Q9KCJ4 Bacillus ResA [Bacillus halodurans] halodurans 1916Manganese-containing catalase UniRef100_Q9KAU6 Bacillus YdbD [Bacillushalodurans] halodurans 1917 BH1562 protein [Bacillus halodurans]UniRef100_Q9KCK9 Bacillus halodurans 1918 Acetyltransferase, GNAT familyUniRef100_Q739K0 Bacillus cereus YjcK [Bacillus cereus] 1919Hypothetical conserved protein UniRef100_Q8ELR7 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 1920 1921 Transcriptional regulator, MarRfamily UniRef100_Q81BM5 Bacillus cereus YkvE [Bacillus cereus] 1922Putative NAD(P)H nitroreductase ydfN UniRef100_P96692 Bacillus subtilisYdfN [Bacillus subtilis] 1923 YdfO protein [Bacillus subtilis]UniRef100_P96693 Bacillus subtilis YdfO 1924 Hypothetical protein[Bacillus cereus UniRef100_Q630S7 Bacillus cereus YwrF ZK] ZK 1925BH1010 protein [Bacillus halodurans] UniRef100_Q9KE48 Bacillus YhcBhalodurans 1926 ORF28 [Staphylococcus phage K] UniRef100_Q6Y7T8Staphylococcus phage K 1927 1928 Thymidylate synthase [BacteriophageUniRef100_P07606 Bacteriophage ThyA phi-3T] phi-3T 1929Sporulation-specific extracellular UniRef100_P42983 Bacillus subtilisNucB nuclease precursor [Bacillus subtilis] 1930 LexA repressor[Bacillus subtilis] UniRef100_P31080 Bacillus subtilis LexA 1931 YneA[Bacillus subtilis] UniRef100_Q45056 Bacillus subtilis YneA 1932 YneB[Bacillus subtilis] UniRef100_Q45057 Bacillus subtilis YneB 1933Hypothetical UPF0291 protein ynzC UniRef100_O31818 Bacillus subtilis[Bacillus subtilis] 1934 Transketolase [Bacillus subtilis]UniRef100_P45694 Bacillus subtilis Tkt 1935 Hypothetical protein yneE[Bacillus UniRef100_P45707 Bacillus subtilis YneE subtilis] 1936Hypothetical UPF0154 protein yneF UniRef100_P45708 Bacillus subtilis[Bacillus subtilis] 1937 1938 Cytochrome c-type biogenesis proteinUniRef100_P45706 Bacillus subtilis CcdA ccdA [Bacillus subtilis] 1939CcdB protein [Bacillus subtilis] UniRef100_P45709 Bacillus subtilis YneI1940 CcdC protein [Bacillus subtilis] UniRef100_P45710 Bacillus subtilisYneJ 1941 Hypothetical protein yneK [Bacillus UniRef100_P45711 Bacillussubtilis YneK subtilis] 1942 Spore coat protein M [Bacillus subtilis]UniRef100_Q45058 Bacillus subtilis CotM 1943 1944 1945 CitB 1946 YneNprotein [Bacillus subtilis] UniRef100_O31820 Bacillus subtilis YneN 19471948 1949 Small, acid-soluble spore protein tlp UniRef100_Q45060Bacillus subtilis [Bacillus subtilis] 1950 YneP [Bacillus subtilis]UniRef100_Q45061 Bacillus subtilis YneP 1951 YneQ [Bacillus subtilis]UniRef100_Q45062 Bacillus subtilis 1952 Hypothetical protein [Bacilluscereus] UniRef100_Q815P1 Bacillus cereus 1953 Conserved domain protein[Bacillus UniRef100_Q72YR7 Bacillus cereus cereus] 1954 YneR [Bacillussubtilis] UniRef100_Q45063 Bacillus subtilis 1955 Hypothetical UPF0078protein yneS UniRef100_Q45064 Bacillus subtilis YneS [Bacillus subtilis]1956 YneT [Bacillus subtilis] UniRef100_Q45065 Bacillus subtilis YneT1957 Topoisomerase IV subunit B [Bacillus UniRef100_Q59192 Bacillussubtilis ParE subtilis] 1958 Topoisomerase IV subunit A [BacillusUniRef100_Q45066 Bacillus subtilis ParC subtilis] 1959 AraR 1960Hypothetical conserved protein UniRef100_Q8EMP2 Oceanobacillus XylB[Oceanobacillus iheyensis] iheyensis 1961 L-ribulose-5-phosphate4-epimerase UniRef100_Q8EMP3 Oceanobacillus AraD [Oceanobacillusiheyensis] iheyensis 1962 L-arabinose isomerase [OceanobacillusUniRef100_Q8EMP4 Oceanobacillus AraA iheyensis] iheyensis 1963 YwtG 1964FabG 1965 Hypothetical protein ynfC [Bacillus UniRef100_Q45067 Bacillussubtilis YnfC subtilis] 1966 Amino acid carrier protein alsT [BacillusUniRef100_Q45068 Bacillus subtilis AlsT subtilis] 1967 NarI 1968 NarJ1969 NarH 1970 NarG 1971 1972 Hypothetical protein yqfB [LactococcusUniRef100_Q9CF70 Lactococcus lactis lactis] 1973 AlbA 1974 ArfM 1975YwiC 1976 Transcriptional regulator of anaerobic UniRef100_Q9KG81Bacillus Fnr genes [Bacillus halodurans] halodurans 1977 Nitriteextrusion protein [Bacillus UniRef100_P46907 Bacillus subtilis NarKsubtilis] 1978 1979 CAMP-binding domains-Catabolite UniRef100_Q8R5P4Thermoanaerobacter Fnr gene activator and regulatory subunit oftengcongensis cAMP-dependent protein kinases [Thermoanaerobactertengcongensis] 1980 Putative nitric oxide reductase UniRef100_Q6GK48Staphylococcus aureus [Staphylococcus aureus] 1981 1982 YngL 1983 BglC1984 Hypothetical protein ynfE [Bacillus UniRef100_Q45069 Bacillussubtilis subtilis] 1985 Hypothetical protein [Bacillus UniRef100_Q9ZF48Bacillus YkkB megaterium] megaterium 1986 Hypothetical protein [BacillusUniRef100_Q70K06 Bacillus amyloliquefaciens amyloliquefaciens] 1987Alkyl hydroperoxide reductase large UniRef100_Q9Z9W3 Bacillus AhpFsubunit [Bacillus halodurans] halodurans 1988 Methyltransferase[Methanosarcina UniRef100_Q8PU82 Methanosarcina mazei mazei] 1989Similar to B. subtilis ywgB gene [Bacillus UniRef100_Q9Z9W2 BacillusYwgB halodurans] halodurans 1990 Hypothetical protein ywoF [BacillusUniRef100_P94576 Bacillus subtilis YwoF subtilis] 1991 Branched-chainamino acid transport UniRef100_P94499 Bacillus subtilis BrnQ systemcarrier protein brnQ [Bacillus subtilis] 1992 NADP-dependent alcoholUniRef100_O06007 Bacillus subtilis AdhA dehydrogenase [Bacillussubtilis] 1993 Transcriptional regulator, MerR family UniRef100_Q721Z3Listeria YraB [Listeria monocytogenes] monocytogenes 1994 HPr-likeprotein crh [Bacillus subtilis] UniRef100_O06976 Bacillus subtilis 1995BH2089 protein [Bacillus halodurans] UniRef100_Q9Z9R4 Bacillus YddRhalodurans 1996 1997 Enoyl-CoA hydratase/isomerase familyUniRef100_Q738L0 Bacillus cereus YngF protein [Bacillus cereus] 1998Hypothetical protein ysiB [Bacillus UniRef100_P94549 Bacillus subtilisYsiB subtilis] 1999 Methylmalonic acid semialdehyde UniRef100_Q63BL0Bacillus cereus MmsA dehydrogenase [Bacillus cereus ZK] ZK 20003-hydroxyisobutyrate dehydrogenase UniRef100_Q63BL1 Bacillus cereus YkwC[Bacillus cereus ZK] ZK 2001 Acyl-CoA dehydrogenase [BacillusUniRef100_Q81DR7 Bacillus cereus YusJ cereus] 2002 Mannose-6-phosphateisomerase UniRef100_O31646 Bacillus subtilis ManA [Bacillus subtilis]2003 Phosphotransferase system (PTS) UniRef100_O31645 Bacillus subtilisManP mannose-specific enzyme IIBCA component [Bacillus subtilis] 20042005 2006 Hypothetical protein [Bacillus cereus] UniRef100_Q72YT6Bacillus cereus 2007 Transcriptional regulator [BacillusUniRef100_O31644 Bacillus subtilis ManR subtilis] 2008 2009UPI00003CC220 UniRef100 entry UniRef100_UPI00003CC220 YtrB 2010Transcriptional regulator [Bacillus UniRef100_Q9KF35 Bacillus YtrAhalodurans] halodurans 2011 Probable oxidoreductase [ClostridiumUniRef100_Q8XP17 Clostridium YjmF perfringens] perfringens 2012Mannonate dehydratase [Clostridium UniRef100_Q8XP15 Clostridium UxuAperfringens] perfringens 2013 Glucosidase [Bacillus halodurans]UniRef100_Q9KEZ5 Bacillus halodurans 2014 C4-dicarboxylate transportsystem UniRef100_Q9KEZ6 Bacillus halodurans [Bacillus halodurans] 2015C4-dicarboxylate transport system UniRef100_Q8EMM5 Oceanobacillusiheyensis permease small protein [Oceanobacillus iheyensis] 2016C4-dicarboxylate transport system UniRef100_Q9KEZ8 Bacillus DctB[Bacillus halodurans] halodurans 2017 Transcriptional regulatorUniRef100_Q8EL22 Oceanobacillus CcpA [Oceanobacillus iheyensis]iheyensis 2018 Arsenate reductase [Bacillus subtilis] UniRef100_P45947Bacillus subtilis ArsC 2019 YdfA protein [Bacillus subtilis]UniRef100_P96678 Bacillus subtilis YdfA 2020 YdeT protein [Bacillussubtilis] UniRef100_P96677 Bacillus subtilis YdeT 2021 YdeI [Bacillushalodurans] UniRef100_Q9Z9R5 Bacillus YdeI halodurans 2022 Putativesecreted protein UniRef100_Q9S1Z5 Streptomyces coelicolor [Streptomycescoelicolor] 2023 Probable glucose uptake protein glcU UniRef100_P40419Bacillus GlcU [Bacillus megaterium] megaterium 2024 YngK protein[Bacillus subtilis] UniRef100_O35015 Bacillus subtilis YngK 2025 YngDprotein [Bacillus subtilis] UniRef100_O31824 Bacillus subtilis YngD 2026Pyruvate formate-lyase-activating UniRef100_Q73DZ6 Bacillus cereus YkvLenzyme [Bacillus cereus] 2027 Formate acetyltransferase [BacillusUniRef100_Q81YX1 Bacillus anthracis anthracis] 2028 DacC 2029 NADHdehydrogenase-like protein yjlD UniRef100_P80861 Bacillus subtilis YjlD[Bacillus subtilis] 2030 Hypothetical protein yjlC [BacillusUniRef100_O34633 Bacillus subtilis YjlC subtilis] 2031 2032 Hypotheticalprotein [Bacillus cereus] UniRef100_Q81IJ8 Bacillus cereus 2033Hypothetical protein ykzH [Bacillus UniRef100_O31653 Bacillus subtilissubtilis] 2034 Acetyl-CoA synthetase [Bacillus UniRef100_Q9KDS4 BacillusAcsA halodurans] halodurans 2035 YngE protein [Bacillus subtilis]UniRef100_O31825 Bacillus subtilis YngE 2036 Hydroxybutyryl-dehydratase[Bacillus UniRef100_Q9L7W1 Bacillus subtilis YngF subtilis] 2037 YngGprotein [Bacillus subtilis] UniRef100_O34873 Bacillus subtilis YngG 2038YngXX [Bacillus subtilis] UniRef100_Q9R9I3 Bacillus subtilis 2039 YngH[Bacillus subtilis] UniRef100_Q9R9I4 Bacillus subtilis YngH 2040 YngI[Bacillus subtilis] UniRef100_Q9R9I5 Bacillus subtilis YngI 2041 YngJprotein [Bacillus subtilis] UniRef100_O34421 Bacillus subtilis YngJ 2042NAD(P)H oxidoreductase YRKL UniRef100_Q7P6P0 Fusobacterium YrkL[Fusobacterium nucleatum subsp. nucleatum vincentii ATCC 49256] subsp.vincentii ATCC 49256 2043 Transcriptional regulator, MarR familyUniRef100_Q8RE85 Fusobacterium nucleatum [Fusobacterium nucleatum] 2044Glutamate-5-semialdehyde UniRef100_Q6HHC2 Bacillus ProA dehydrogenase[Bacillus thuringiensis] thuringiensis 2045 Glutamate 5-kinase 2[Bacillus subtilis] UniRef100_O07509 Bacillus subtilis ProJ 2046Pyrroline-5-carboxylate reductase 1 UniRef100_P14383 Bacillus subtilisProH [Bacillus subtilis] 2047 UPI00003CB6CD UniRef100 entryUniRef100_UPI00003CB6CD YetF 2048 Sodium-dependent phosphateUniRef100_Q9KCT1 Bacillus CysP transporter [Bacillus halodurans]halodurans 2049 Probable phosphoadenosine UniRef100_O06737 Bacillussubtilis YitB phosphosulfate reductase [Bacillus subtilis] 2050Phosphosulfolactate synthase (EC UniRef100_O06739 Bacillus subtilis YitD4.4.1.19) ((2R)-phospho-3-sulfolactate synthase) [Bacillus subtilis]2051 YitE [Bacillus subtilis] UniRef100_O06740 Bacillus subtilis YitE2052 YitF [Bacillus subtilis] UniRef100_O06741 Bacillus subtilis YitF2053 YitG [Bacillus subtilis] UniRef100_O06742 Bacillus subtilis YitG2054 Putative glycosyl transferase ykoT UniRef100_O34755 Bacillussubtilis YkoT [Bacillus subtilis] 2055 YkoR [Bacillus subtilis]UniRef100_O34830 Bacillus subtilis YkoS 2056 Glutamate synthase [NADPH]small UniRef100_O34399 NADPH GltB chain [Bacillus subtilis] 2057 GltA2058 HTH-type transcriptional regulator gltC UniRef100_P20668 Bacillussubtilis GltC [Bacillus subtilis] 2059 AII7121 protein [Anabaena sp.]UniRef100_Q8YL17 Anabaena sp. 2060 2061 Lmo0606 protein [ListeriaUniRef100_Q8Y9C6 Listeria monocytogenes monocytogenes] 2062 ABCtransporter ATP-binding protein UniRef100_Q67MU2 Symbiobacterium YfiB[Symbiobacterium thermophilum] thermophilum 2063 ABC transporterATP-binding protein UniRef100_Q67MU3 Symbiobacterium YfiC[Symbiobacterium thermophilum] thermophilum 2064 Cytochrome P450 109[Bacillus subtilis] UniRef100_P27632 Bacillus subtilis YjiB 2065Hypothetical oxidoreductase yoxD UniRef100_P14802 Bacillus subtilis YoxD[Bacillus subtilis] 2066 Pps 2067 Permease, general substrateUniRef100_Q6HMC3 Bacillus LmrB transporter [Bacillus thuringiensis]thuringiensis 2068 Putative formate dehydrogenase UniRef100_O34323Bacillus subtilis YoaE [Bacillus subtilis] 2069 Transcriptionalregulatory protein UniRef100_Q89KD1 Bradyrhizobium YcgE [Bradyrhizobiumjaponicum] japonicum 2070 Drug resistance transporter, UniRef100_Q736I5Bacillus cereus Mdr EmrB/QacA family [Bacillus cereus] 2071 YndE protein[Bacillus subtilis] UniRef100_O31809 Bacillus subtilis YndE 2072 YndFprotein [Bacillus subtilis] UniRef100_O31810 Bacillus subtilis YndF 2073YndE 2074 YndE 2075 UPI00003CB22E UniRef100 entryUniRef100_UPI00003CB22E YndD 2076 2077 Nucleotide binding protein expZUniRef100_P39115 Bacillus subtilis ExpZ [Bacillus subtilis] 2078 TlpB2079 CsaA protein [Bacillus subtilis] UniRef100_P37584 Bacillus subtilisCsaA 2080 Alkaline serine protease [Bacillus UniRef100_O31788 Bacillussubtilis AprX subtilis] 2081 Hypothetical protein [Bacillus anthracis]UniRef100_Q81SD4 Bacillus YnzE anthracis 2082 Transcriptional regulator,TetR family UniRef100_Q63D70 Bacillus cereus YrhI [Bacillus cereus ZK]ZK 2083 Putative HTH-type transcriptional UniRef100_P40762 Bacillussubtilis YvmB regulator yvmB [Bacillus subtilis] 2084 Hydrolase[Bacillus cereus] UniRef100_Q81D79 Bacillus cereus YqeK 2085Hypothetical conserved protein UniRef100_Q8ETG3 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 2086 YndJ protein [Bacillus subtilis]UniRef100_O31813 Bacillus subtilis YndJ 2087 YndH protein [Bacillussubtilis] UniRef100_O31812 Bacillus subtilis YndH 2088 Hypotheticalprotein [Bacillus cereus] UniRef100_Q73A97 Bacillus cereus YndG 2089UPI00003CBA97 UniRef100 entry UniRef100_UPI00003CBA97 YobS 2090UPI00003CBA98 UniRef100 entry UniRef100_UPI00003CBA98 YobT 2091DNA-binding protein YobU [Bacillus UniRef100_O34637 Bacillus subtilisYobU subtilis] 2092 Hypothetical protein [Bacteroides UniRef100_Q64RP1Bacteroides fragilis fragilis] 2093 Transcription regulator [Bacillussubtilis] UniRef100_O34920 Bacillus subtilis YobV 2094 YobW 2095 YozAprotein [Bacillus subtilis] UniRef100_O31844 Bacillus subtilis 2096Possible metallo-beta-lactamase family UniRef100_Q638G1 Bacillus cereusYmaE protein [Bacillus cereus ZK] ZK 2097 2098 YfnA 2099 YocA 2100UPI00003CBE6E UniRef100 entry UniRef100_UPI00003CBE6E YveM 2101Hypothetical protein [Bacillus cereus] UniRef100_Q817C2 Bacillus cereus2102 2103 Glycosyl transferase [Bacillus UniRef100_Q9K7I1 Bacillus YtcChalodurans] halodurans 2104 YozB protein [Bacillus subtilis]UniRef100_O31845 Bacillus subtilis YozB 2105 Lmo2079 protein [ListeriaUniRef100_Q8Y5I3 Listeria monocytogenes monocytogenes] 2106 YocC[Bacillus subtilis] UniRef100_O35042 Bacillus subtilis YocC 2107Na+/myo-inositol cotransporter [Bacillus UniRef100_Q9KAR5 Bacillus YcgOhalodurans] halodurans 2108 YocH [Bacillus subtilis] UniRef100_O34669Bacillus subtilis YocH 2109 RecQ homolog [Bacillus subtilis]UniRef100_O34748 Bacillus subtilis YocI 2110 Hypothetical protein yqbC[Bacillus UniRef100_P45919 Bacillus subtilis YqbC subtilis] 2111Hypothetical protein yjfB [Bacillus UniRef100_O34438 Bacillus subtilissubtilis] 2112 Hypothetical protein yyaQ [Bacillus UniRef100_P37507Bacillus subtilis YyaQ subtilis] 2113 Hypothetical protein OB2103UniRef100_Q8EPJ8 Oceanobacillus iheyensis [Oceanobacillus iheyensis]2114 2115 Hypothetical protein yjgD [Bacillus UniRef100_O34681 Bacillussubtilis YjgD subtilis] 2116 YjgC 2117 YjgC 2118 Hypothetical proteinOB3361 UniRef100_Q8EL70 Oceanobacillus iheyensis [Oceanobacillusiheyensis] 2119 Hypothetical protein ypfA [Bacillus UniRef100_P38491Bacillus subtilis YpfA subtilis] 2120 2121 2122 YxiB 2123 2124 2125 2126YndF 2127 2128 Hypothetical protein [Bacillus cereus] UniRef100_Q816C2Bacillus cereus 2129 BH0185 protein [Bacillus halodurans]UniRef100_Q9KGB9 Bacillus halodurans 2130 2131 YisY 2132 Hypotheticalprotein yoqH UniRef100_O64117 Bacteriophage YoqH [Bacteriophage SPBc2]SPBc2 2133 2134 BH0429 protein [Bacillus halodurans] UniRef100_Q9KFP9Bacillus YrhP halodurans 2135 30S ribosomal protein S14 UniRef100_Q8ETX0Oceanobacillus iheyensis [Oceanobacillus iheyensis] 2136 UPI00002DEBB5UniRef100 entry UniRef100_UPI00002DEBB5 MutT 2137 Pyruvate waterdikinase UniRef100_Q8TN35 Methanosarcina Pps [Methanosarcinaacetivorans] acetivorans 2138 Transcriptional regulator UniRef100_Q8ESJ8Oceanobacillus YxbF [Oceanobacillus iheyensis] iheyensis 2139 2140 YndMprotein [Bacillus subtilis] UniRef100_O31816 Bacillus subtilis YndM 2141Hypothetical protein yisT [Bacillus UniRef100_O07939 Bacillus subtilisYisT subtilis] 2142 Putative acyl carrier protein UniRef100_O35022Bacillus subtilis YocJ phosphodiesterase 1 [Bacillus subtilis] 2143General stress protein 16O [Bacillus UniRef100_P80872 Bacillus subtilisYocK subtilis] 2144 2145 2146 Aldehyde dehydrogenase [BacillusUniRef100_O34660 Bacillus subtilis DhaS subtilis] 2147 YjbB protein[Bacillus subtilis] UniRef100_O31600 Bacillus subtilis YjbB 2148Aminoglycoside N6′-acetyltransferase UniRef100_Q81AT3 Bacillus cereusYjcK [Bacillus cereus] 2149 Blasticidin S deaminase, putativeUniRef100_Q81Y61 Bacillus anthracis [Bacillus anthracis] 2150 SqhC 2151Probable superoxide dismutase [Fe] UniRef100_O35023 Fe SodF [Bacillussubtilis] 2152 Stress response protein yvgO precursor UniRef100_O32211Bacillus subtilis YvgO [Bacillus subtilis] 2153 Putative transporter[Bacillus subtilis] UniRef100_O34383 Bacillus subtilis YocR 2154Putative transporter [Bacillus subtilis] UniRef100_O34524 Bacillussubtilis YocS 2155 Dihydrolipoyllysine-residue UniRef100_P16263 Bacillussubtilis OdhB succinyltransferase component of 2- oxoglutaratedehydrogenase complex [Bacillus subtilis] 2156 2-oxoglutaratedehydrogenase E1 UniRef100_P23129 Bacillus subtilis OdhA component[Bacillus subtilis] 2157 YojO protein [Bacillus subtilis]UniRef100_O31849 Bacillus subtilis YojO 2158 YojN protein [Bacillussubtilis] UniRef100_O31850 Bacillus subtilis YojN 2159 Hypotheticalsuperoxide dismutase-like UniRef100_O31851 Bacillus subtilis YojMprotein yojM precursor [Bacillus subtilis] 2160 Hypothetical proteinyojL precursor UniRef100_O31852 Bacillus subtilis LytF [Bacillussubtilis] 2161 Probable multidrug resistance protein UniRef100_O31855Bacillus subtilis YojI norM (Na(+)/drug antiporter) [Bacillus subtilis]2162 Hypothetical protein [Bacillus cereus UniRef100_Q637Z8 Bacilluscereus YojG ZK] ZK 2163 YojF protein [Bacillus subtilis]UniRef100_O31858 Bacillus subtilis YojF 2164 YojE [Bacillus subtilis]UniRef100_O68260 Bacillus subtilis 2165 YojE protein [Bacillus subtilis]UniRef100_O31859 Bacillus subtilis YojE 2166 Hypothetical protein yozR[Bacillus UniRef100_Q7WY67 Bacillus subtilis YozR subtilis] 2167 YoaJ[Bacillus subtilis] UniRef100_O34918 Bacillus subtilis YoaJ 2168 2169UPI00003CBE9B UniRef100 entry UniRef100_UPI00003CBE9B YwfD 2170Hypothetical UPF0087 protein yodB UniRef100_O34844 Bacillus subtilisYodB [Bacillus subtilis] 2171 Putative NAD(P)H nitroreductase 12CUniRef100_P81102 Bacillus subtilis YodC [Bacillus subtilis] 2172 YolF[Bacillus subtilis] UniRef100_O34842 Bacillus subtilis YodD 2173UPI0000315ACC UniRef100 entry UniRef100_UPI0000315ACC 2174 YodF protein[Bacillus subtilis] UniRef100_O34745 Bacillus subtilis YodF 2175IS1627s1-related, transposase [Bacillus UniRef100_Q7CMD0 Bacillusanthracis str. A2012 anthracis str. A2012] 2176 UPI00003CC069 UniRef100entry UniRef100_UPI00003CC069 2177 OrfRM1 [Bacillus subtilis]UniRef100_O34666 Bacillus subtilis CtpA 2178 YolB [Bacillus subtilis]UniRef100_O34954 Bacillus subtilis YodH 2179 2180 Carboxypeptidase[Bacillus subtilis] UniRef100_O34866 Bacillus subtilis YodJ 2181 Purinenucleoside phosphorylase II UniRef100_O34925 Bacillus subtilis DeoD[Bacillus subtilis] 2182 Hypothetical Membrane Spanning UniRef100_Q813P0Bacillus cereus YcgR Protein [Bacillus cereus] 2183 YcgQ protein[Bacillus subtilis] UniRef100_P94394 Bacillus subtilis YcgQ 2184 2185Hypothetical protein yodL [Bacillus UniRef100_O30472 Bacillus subtilisYodL subtilis] 2186 YodM 2187 Hypothetical protein yozD [BacillusUniRef100_O31863 Bacillus subtilis subtilis] 2188 Hypothetical proteinyodN [Bacillus UniRef100_O34414 Bacillus subtilis YodN subtilis] 21892190 YokU [Bacillus subtilis] UniRef100_O30470 Bacillus subtilis 2191Hypothetical UPF0069 protein yodO UniRef100_O34676 Bacillus subtilisKamA [Bacillus subtilis] 2192 YodP [Bacillus subtilis] UniRef100_O34895Bacillus subtilis YodP 2193 Acetylornitine deacetylase [BacillusUniRef100_O34984 Bacillus subtilis YodQ subtilis] 2194Butirate-acetoacetate CoA transferase UniRef100_O34466 Bacillus subtilisYodR [Bacillus subtilis] 2195 Butyrate acetoacetate-CoA transferaseUniRef100_O34317 Bacillus subtilis YodS [Bacillus subtilis] 2196Probable aminotransferase yodT UniRef100_O34662 Bacillus subtilis YodT[Bacillus subtilis] 2197 Multidrug resistance protein; possibleUniRef100_Q6HK46 Bacillus YkuC tetracycline resistance determinantthuringiensis [Bacillus thuringiensis] 2198 Protein cgeE [Bacillussubtilis] UniRef100_P42093 Bacillus subtilis CgeE 2199 Peptidemethionine sulfoxide reductase UniRef100_P54155 Bacillus subtilis YppQmsrB [Bacillus subtilis] 2200 MsrA 2201 Putative HTH-typetranscriptional UniRef100_P54182 Bacillus subtilis YpoP regulator ypoP[Bacillus subtilis] 2202 2203 Hypothetical protein yhcK [BacillusUniRef100_P54595 Bacillus subtilis YhcK subtilis] 2204 Hypotheticalprotein ypnP [Bacillus UniRef100_P54181 Bacillus subtilis YpnP subtilis]2205 Hypothetical conserved protein UniRef100_Q746K9 Thermusthermophilus [Thermus thermophilus] 2206 2207 Hypothetical protein ypmS[Bacillus UniRef100_P54179 Bacillus subtilis YpmS subtilis] 2208Hypothetical protein ypmR [Bacillus UniRef100_P40766 Bacillus subtilisYpmR subtilis] 2209 Hypothetical protein ypmQ [Bacillus UniRef100_P54178Bacillus subtilis YpmQ subtilis] 2210 DegV family protein [Bacilluscereus UniRef100_Q63BU6 Bacillus cereus YviA ZK] ZK 2211 Hypotheticalprotein ypmP [Bacillus UniRef100_P54177 Bacillus subtilis subtilis] 2212Threonine dehydratase biosynthetic UniRef100_P37946 Bacillus subtilisIlvA [Bacillus subtilis] 2213 Putative sigma L-dependentUniRef100_P54156 Bacillus subtilis YplP transcriptional regulator yplP[Bacillus subtilis] 2214 Hemolysin III homolog [Bacillus subtilis]UniRef100_P54175 Bacillus subtilis YplQ 2215 Hypothetical protein ypkP[Bacillus UniRef100_P54174 Bacillus subtilis YpkP subtilis] 2216Dihydrofolate reductase [Bacillus UniRef100_P11045 Bacillus subtilisDfrA subtilis] 2217 Hypothetical protein ypjQ [Bacillus UniRef100_P54173Bacillus subtilis YpjQ subtilis] 2218 YpjP 2219 YpiP 2220 Hypotheticalprotein yphP [Bacillus UniRef100_P54170 Bacillus subtilis YphP subtilis]2221 Dihydroxy-acid dehydratase [Bacillus UniRef100_P51785 Bacillussubtilis IlvD subtilis] 2222 YpgR 2223 Hypothetical protein ypgQ[Bacillus UniRef100_P54168 Bacillus subtilis YpgQ subtilis] 2224Glutathione peroxidase homolog bsaA UniRef100_P52035 Bacillus subtilisBsaA [Bacillus subtilis] 2225 UPI00003CBA0F UniRef100 entryUniRef100_UPI00003CBA0F 2226 Homoserine O-succinyltransferaseUniRef100_P54167 Bacillus subtilis MetA [Bacillus subtilis] 2227Putative glycosyl transferase ypfP UniRef100_P54166 Bacillus subtilisUgtP [Bacillus subtilis] 2228 2229 Cold shock protein cspD [BacillusUniRef100_P51777 Bacillus subtilis subtilis] 2230 Regulatory proteindegR [Bacillus UniRef100_P06563 Bacillus subtilis subtilis] 2231Hypothetical protein ypzA [Bacillus UniRef100_O32007 Bacillus subtilissubtilis] 2232 2233 Hypothetical protein ypeP [Bacillus UniRef100_P54164Bacillus subtilis YpeP subtilis] 2234 Hypothetical protein ypdP[Bacillus UniRef100_P54163 Bacillus subtilis YpdP subtilis] 2235 14.7kDa ribonuclease H-like protein UniRef100_P54162 Bacillus subtilis YpdQ[Bacillus subtilis] 2236 Probable 5′-3′ exonuclease [BacillusUniRef100_P54161 Bacillus subtilis YpcP subtilis] 2237 2238 Hypotheticalprotein ypbS [Bacillus UniRef100_P54160 Bacillus subtilis subtilis] 2239Hypothetical protein ypbR [Bacillus UniRef100_P54159 Bacillus subtilisYpbR subtilis] 2240 2241 Hypothetical protein ypbQ [BacillusUniRef100_P54158 Bacillus subtilis YpbQ subtilis] 2242 BcsA 2243Predicted acetyltransferase [Clostridium UniRef100_Q97G03 ClostridiumYokL acetobutylicum] acetobutylicum 2244 Xanthine permease [Bacillussubtilis] UniRef100_P42086 Bacillus subtilis PbuX 2245 Xanthinephosphoribosyltransferase UniRef100_P42085 Bacillus subtilis Xpt[Bacillus subtilis] 2246 Hypothetical metalloprotease ypwAUniRef100_P50848 Bacillus subtilis YpwA [Bacillus subtilis] 2247 YpvA2248 Hypothetical protein yptA precursor UniRef100_P50841 Bacillussubtilis [Bacillus subtilis] 2249 2250 Hypothetical UPF0020 protein ypsCUniRef100_P50840 Bacillus subtilis YpsC precursor [Bacillus subtilis]2251 Hypothetical protein ypsB [Bacillus UniRef100_P50839 Bacillussubtilis subtilis] 2252 Hypothetical protein ypsA [BacillusUniRef100_P50838 Bacillus subtilis YpsA subtilis] 2253 Spore coatprotein D [Bacillus subtilis] UniRef100_P07791 Bacillus subtilis 22542255 YprB 2256 YprA 2257 Putative PTS system IIA componentUniRef100_P50829 Bacillus subtilis YpqE ypqE [Bacillus subtilis] 2258Hypothetical protein ypqA precursor UniRef100_P50836 Bacillus subtilisYpqA [Bacillus subtilis] 2259 Hypothetical protein yppG [BacillusUniRef100_P50835 Bacillus subtilis YppG subtilis] 2260 2261 YppE 22622263 2264 Hypothetical protein yppC [Bacillus UniRef100_P39791 Bacillussubtilis YppC subtilis] 2265 Recombination protein U [BacillusUniRef100_P39792 Bacillus subtilis RecU subtilis] 2266Penicillin-binding protein 1A/1B (PBP1) UniRef100_P39793 Includes: PonA[Includes: Penicillin-insensitive Penicillin- transglycosylase (EC2.4.2.—) insensitive (Peptidoglycan TGase): Penicillin- transglycosylasesensitive transpeptidase (EC 3.4.—.—) (EC 2.4.2.—) (DD-transpeptidase)][Bacillus subtilis] (Peptidoglycan TGase); Penicillin- sensitivetranspeptidase (EC 3.4.—.—) (DD- transpeptidase) 2267 Hypotheticalprotein ypoC [Bacillus UniRef100_P39789 Bacillus subtilis YpoC subtilis]2268 Probable endonuclease III (EC UniRef100_P39788 Bacillus subtilisNth 4.2.99.18) (DNA-(apurinic or apyrimidinic site) lyase) [Bacillussubtilis] 2269 DNA replication protein dnaD [Bacillus UniRef100_P39787Bacillus subtilis DnaD subtilis] 2270 Asparaginyl-tRNA synthetase[Bacillus UniRef100_P39772 Bacillus subtilis AsnS subtilis] 2271Aspartate aminotransferase [Bacillus UniRef100_P53001 Bacillus subtilisAspB subtilis] 2272 Hypothetical protein ypmB [Bacillus UniRef100_P54396Bacillus subtilis YpmB subtilis] 2273 Hypothetical protein ypmA[Bacillus UniRef100_P54395 Bacillus subtilis subtilis] 2274 ProbableATP-dependent helicase dinG UniRef100_P54394 Bacillus subtilis DinGhomolog [Bacillus subtilis] 2275 Aspartate 1-decarboxylase precursorUniRef100_P52999 Bacillus subtilis PanD [Bacillus subtilis] 2276Pantoate--beta-alanine ligase [Bacillus UniRef100_P52998 Bacillussubtilis PanC subtilis] 2277 3-methyl-2-oxobutanoate UniRef100_P52996Bacillus subtilis PanB hydroxymethyltransferase [Bacillus subtilis] 2278BirA bifunctional protein [Includes: UniRef100_P42975 Includes: BiotinBirA Biotin operon repressor; Biotin--[acetyl- operon CoA-carboxylase]synthetase (EC repressor; Biotin-- 6.3.4.15) (Biotin--protein ligase)][Bacillus subtilis] 2279 Poly(A) polymerase [Bacillus subtilis]UniRef100_P42977 Bacillus subtilis Cca 2280 Putative glycosyltransferase ypjH UniRef100_P42982 Bacillus subtilis YpjH [Bacillussubtilis] 2281 Hypothetical protein ypjG [Bacillus UniRef100_P42981Bacillus subtilis YpjG subtilis] 2282 Methylglyoxal synthase [BacillusUniRef100_P42980 Bacillus subtilis MgsA subtilis] 2283Dihydrodipicolinate reductase [Bacillus UniRef100_P42976 Bacillussubtilis DapB subtilis] 2284 Hypothetical protein ypjD [BacillusUniRef100_P42979 Bacillus subtilis YpjD subtilis] 2285 YpjC 2286Hypothetical protein ypjB precursor UniRef100_P54393 Bacillus subtilisYpjB [Bacillus subtilis] 2287 YpjA 2288 QcrC 2289 Menaquinol-cytochromec reductase UniRef100_P46912 Bacillus subtilis QcrB cytochrome b subunit[Bacillus subtilis] 2290 Menaquinol-cytochrome c reductaseUniRef100_P46911 Bacillus subtilis QcrA iron-sulfur subunit [Bacillussubtilis] 2291 Hypothetical protein ypiF [Bacillus UniRef100_P54391Bacillus subtilis YpiF subtilis] 2292 Hypothetical UPF0302 protein ypiBUniRef100_P54390 Bacillus subtilis YpiB [Bacillus subtilis] 2293Hypothetical protein ypiA [Bacillus UniRef100_P54389 Bacillus subtilisYpiA subtilis] 2294 3-phosphoshikimate 1- UniRef100_P20691 Bacillussubtilis AroE carboxyvinyltransferase [Bacillus subtilis] 2295Prephenate dehydrogenase [Bacillus UniRef100_P20692 Bacillus subtilisTyrA subtilis] 2296 HisC 2297 Tryptophan synthase alpha chainUniRef100_P07601 Bacillus subtilis TrpA [Bacillus subtilis] 2298Tryptophan synthase beta chain UniRef100_P07600 Bacillus subtilis TrpB[Bacillus subtilis] 2299 N-(5′-phosphoribosyl)anthranilateUniRef100_P20167 Bacillus subtilis TrpF isomerase [Bacillus subtilis]2300 Indole-3-glycerol phosphate synthase UniRef100_P03964 Bacillussubtilis TrpC [Bacillus subtilis] 2301 Anthranilatephosphoribosyltransferase UniRef100_P03947 Bacillus subtilis TrpD[Bacillus subtilis] 2302 Anthranilate synthase component IUniRef100_P03963 Bacillus subtilis TrpE [Bacillus subtilis] 2303Chorismate mutase [Bacillus subtilis] UniRef100_P19080 Bacillus subtilisAroH 2304 3-dehydroquinate synthase [Bacillus UniRef100_P31102 Bacillussubtilis AroB subtilis] 2305 Chorismate synthase [Bacillus subtilis]UniRef100_P31104 Bacillus subtilis AroF 2306 Chemotaxis proteinmethyltransferase UniRef100_P31105 Bacillus subtilis CheR [Bacillussubtilis] 2307 Nucleoside diphosphate kinase UniRef100_P31103 Bacillussubtilis Ndk [Bacillus subtilis] 2308 Heptaprenyl diphosphate synthaseUniRef100_P31114 Bacillus subtilis HepT component II [Bacillus subtilis]2309 Menaquinone biosynthesis UniRef100_P31113 Bacillus subtilis MenHmethyltransferase ubiE [Bacillus subtilis] 2310 Heptaprenyl diphosphatesynthase UniRef100_P31112 Bacillus subtilis HepS component I [Bacillussubtilis] 2311 Transcription attenuation protein mtrB UniRef100_P19466Bacillus subtilis [Bacillus subtilis] 2312 GTP cyclohydrolase I[Bacillus subtilis] UniRef100_P19465 Bacillus subtilis MtrA 2313DNA-binding protein HU 1 [Bacillus UniRef100_P08821 Bacillus subtilissubtilis] 2314 Stage IV sporulation protein A [Bacillus UniRef100_P35149Bacillus subtilis SpoIVA subtilis] 2315 Hypothetical protein yphF[Bacillus UniRef100_P39911 Bacillus subtilis YphF subtilis] 2316Hypothetical protein yphE [Bacillus UniRef100_P50744 Bacillus subtilissubtilis] 2317 Glycerol-3-phosphate dehydrogenase UniRef100_P46919NAD(P)+ GpsA [NAD(P)+] (EC 1.1.1.94) (NAD(P)H- dependentglycerol-3-phosphate dehydrogenase) (NAD(P)H-dependentdihydroxyacetone-phosphate reductase) [Bacillus subtilis] 2318 YphC 2319Hypothetical protein OB1798 UniRef100_Q8EQA7 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 2320 Hypothetical protein yphB [BacillusUniRef100_P50742 Bacillus subtilis SeaA subtilis] 2321 Hypotheticalprotein yphA [Bacillus UniRef100_P50741 Bacillus subtilis YphA subtilis]2322 2323 YpgA 2324 30S ribosomal protein S1 homolog UniRef100_P38494Bacillus subtilis YpfD [Bacillus subtilis] 2325 Cytidylate kinase[Bacillus subtilis] UniRef100_P38493 Bacillus subtilis Cmk 2326Hypothetical protein ypfB [Bacillus UniRef100_P38492 Bacillus subtilissubtilis] 2327 Sporulation protein ypeB [Bacillus UniRef100_P38490Bacillus subtilis YpeB subtilis] 2328 Spore cortex-lytic enzymeprecursor UniRef100_P50739 Bacillus subtilis SleB [Bacillus subtilis]2329 YpdC 2330 YccC 2331 Hypothetical protein ypdA [BacillusUniRef100_P50736 Bacillus subtilis YpdA subtilis] 2332 NAD-specificglutamate dehydrogenase UniRef100_P50735 Bacillus subtilis GudB[Bacillus subtilis] 2333 Adapter protein mecA 2 [BacillusUniRef100_P50734 Bacillus subtilis YpbH subtilis] 2334 Hypotheticalprotein ypbG precursor UniRef100_P50733 Bacillus subtilis YpbG [Bacillussubtilis] 2335 YpbF 2336 Hypothetical protein ypbE [BacillusUniRef100_P50731 Bacillus subtilis YpbE subtilis] 2337 Hypotheticalprotein ypbD [Bacillus UniRef100_P50730 Bacillus subtilis YpbD subtilis]2338 ATP-dependent DNA helicase recQ UniRef100_P50729 Bacillus subtilisRecQ [Bacillus subtilis] 2339 Hypothetical protein ypbB [BacillusUniRef100_P50728 Bacillus subtilis YpbB subtilis] 2340 Ferredoxin[Bacillus subtilis] UniRef100_P50727 Bacillus subtilis 2341 Hypotheticalprotein ypaA [Bacillus UniRef100_P50726 Bacillus subtilis YpaA subtilis]2342 2343 D-3-phosphoglycerate dehydrogenase UniRef100_P35136 Bacillussubtilis SerA [Bacillus subtilis] 2344 BH1600 protein [Bacillushalodurans] UniRef100_Q9KCH1 Bacillus halodurans 2345 Sigma-X negativeeffector [Bacillus UniRef100_P35166 Bacillus subtilis RsiX subtilis]2346 RNA polymerase sigma factor sigX UniRef100_P35165 Bacillus subtilisSigX [Bacillus subtilis] 2347 Transcriptional regulator [BacillusUniRef100_Q72XJ3 Bacillus cereus LytR cereus] 2348Endo-1,4-beta-xylanase [Bacillus UniRef100_Q9K630 Bacillus YheNhalodurans] halodurans 2349 2350 Alkaline phosphatase synthesis sensorUniRef100_Q898N3 Clostridium tetani YclK protein phoR [Clostridiumtetani] 2351 Response regulators consisting of a UniRef100_Q8R9H7Thermoanaerobacter YycF CheY-like receiver domain and a HTHtengcongensis DNA-binding domain [Thermoanaerobacter tengcongensis] 2352Sensor protein resE [Bacillus subtilis] UniRef100_P35164 Bacillussubtilis ResE 2353 Transcriptional regulatory protein resDUniRef100_P35163 Bacillus subtilis ResD [Bacillus subtilis] 2354 ProteinresC [Bacillus subtilis] UniRef100_P35162 Bacillus subtilis ResC 2355ResB protein [Bacillus subtilis] UniRef100_P35161 Bacillus subtilis ResB2356 Thiol-disulfide oxidoreductase resA UniRef100_P35160 Bacillussubtilis ResA [Bacillus subtilis] 2357 Ribosomal large subunitpseudouridine UniRef100_P35159 Bacillus subtilis RluB synthase B[Bacillus subtilis] 2358 Spore maturation protein B [BacillusUniRef100_P35158 Bacillus subtilis SpmB subtilis] 2359 Spore maturationprotein A [Bacillus UniRef100_P35157 Bacillus subtilis SpmA subtilis]2360 Penicillin-binding protein 5* precursor UniRef100_P35150 Bacillussubtilis DacB [Bacillus subtilis] 2361 Hypothetical protein ypuI[Bacillus UniRef100_P35156 Bacillus subtilis YpuI subtilis] 2362Segregation and condensation protein UniRef100_P35155 Bacillus subtilisYpuH B [Bacillus subtilis] 2363 Segregation and condensation proteinUniRef100_P35154 Bacillus subtilis YpuG A [Bacillus subtilis] 2364 2365Hypothetical protein ypuF [Bacillus UniRef100_P17617 Bacillus subtilisYpuF subtilis] 2366 RibT protein [Bacillus subtilis] UniRef100_P17622Bacillus subtilis RibT 2367 6,7-dimethyl-8-ribityllumazine synthaseUniRef100_Q44681 Bacillus RibH [Bacillus amyloliquefaciens]amyloliquefaciens 2368 Riboflavin biosynthesis protein ribAUniRef100_P17620 Includes: GTP RibA [Includes: GTP cyclohydrolase II (ECcyclohydrolase II 3.5.4.25); 3,4-dihydroxy-2-butanone 4- (EC 3.5.4.25);phosphate synthase (DHBP synthase)] 3,4-dihydroxy-2- [Bacillus subtilis]butanone 4- phosphate synthase (DHBP synthase) 2369 Riboflavin synthasealpha chain UniRef100_P16440 Bacillus subtilis RibE [Bacillus subtilis]2370 Riboflavin biosynthesis protein ribD UniRef100_P17618 Includes:RibD [Includes: Diaminohydroxy-Diaminohydroxyphosphoribosylaminopyrimidinephosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) deaminase (EC3.5.4.26) (Riboflavin-specific deaminase); 5- (Riboflavin-amino-6-(5-phosphoribosylamino)uracil specific reductase (EC 1.1.1.193)(HTP deaminase); 5- reductase)] [Bacillus subtilis] amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193) (HTP reductase) 2371Hypothetical protein ypuD [Bacillus UniRef100_P17616 Bacillus subtilisYpuD subtilis] 2372 Putative serine/threonine protein UniRef100_Q7VFC1Helicobacter YjbP phosphatase [Helicobacter hepaticus] hepaticus 2373Stress response homolog Hsp [Bacillus UniRef100_Q9X3Z5 Bacillus subtilissubtilis] 2374 Response regulator aspartate UniRef100_Q00828 Bacillussubtilis RapA phosphatase A [Bacillus subtilis] 2375 Peptidyl-prolylcis-trans isomerase B UniRef100_P35137 Bacillus subtilis PpiB [Bacillussubtilis] 2376 YpuA 2377 IS1627s1-related, transposase [BacillusUniRef100_Q7CMD0 Bacillus anthracis str. A2012 anthracis str. A2012]2378 UPI00003CC069 UniRef100 entry UniRef100_UPI00003CC069 2379 YndL2380 Diaminopimelate decarboxylase UniRef100_P23630 Bacillus subtilisLysA [Bacillus subtilis] 2381 2382 Stage V sporulation protein AF[Bacillus UniRef100_P31845 Bacillus subtilis SpoVAF subtilis] 2383 StageV sporulation protein AE [Bacillus UniRef100_P40870 Bacillus subtilisSpoVAE subtilis] 2384 Stage V sporulation protein AE [BacillusUniRef100_P40870 Bacillus subtilis SpoVAE subtilis] 2385 Stage Vsporulation protein AD UniRef100_P40869 Bacillus subtilis SpoVAD[Bacillus subtilis] 2386 SpoVAC 2387 Stage V sporulation protein AB[Bacillus UniRef100_P40867 Bacillus subtilis SpoVAB subtilis] 2388 StageV sporulation protein AA [Bacillus UniRef100_P40866 Bacillus subtilisSpoVAA subtilis] 2389 SigF 2390 SpoIIAB 2391 SpoIIAA 2392Penicillin-binding protein dacF UniRef100_P38422 Bacillus subtilis DacFprecursor [Bacillus subtilis] 2393 Purine nucleoside phosphorylase IUniRef100_P46354 Bacillus subtilis PunA [Bacillus subtilis] 2394Phosphopentomutase [Bacillus subtilis] UniRef100_P46353 Bacillussubtilis Drm 2395 Tyrosine recombinase xerD [Bacillus UniRef100_P46352Bacillus subtilis RipX subtilis] 2396 2397 Ferric uptake regulationprotein UniRef100_P54574 Bacillus subtilis Fur [Bacillus subtilis] 2398Stage II sporulation protein M [Bacillus UniRef100_P37873 Bacillussubtilis SpoIIM subtilis] 2399 Lmo2763 protein [ListeriaUniRef100_Q926Y4 Listeria YdhO monocytogenes] monocytogenes 2400UPI00003CA2F1 UniRef100 entry UniRef100_UPI00003CA2F1 2401 Probableallantoin permease [Bacillus UniRef100_P94575 Bacillus subtilis YwoEsubtilis] 2402 YdaF 2403 Hypothetical protein yqkK [BacillusUniRef100_P54573 Bacillus subtilis subtilis] 2404 YybD 2405 Hypotheticalprotein [Bacillus pumilus] UniRef100_Q93PN4 Bacillus pumilus YqxK 2406ADP-ribose pyrophosphatase [Bacillus UniRef100_P54570 Bacillus subtilisNudF subtilis] 2407 2408 YdgC protein [Bacillus subtilis]UniRef100_P96701 Bacillus subtilis YdgC 2409 Hypothetical protein ydgD[Bacillus UniRef100_P96702 Bacillus subtilis YdgD subtilis] 2410Hypothetical oxidoreductase yqkF UniRef100_P54569 Bacillus subtilis YqkF[Bacillus subtilis] 2411 2412 YqkD 2413 Hypothetical protein yqkC[Bacillus UniRef100_P54566 Bacillus subtilis subtilis] 2414 Hypotheticalprotein yqkB [Bacillus UniRef100_P54565 Bacillus subtilis YqkB subtilis]2415 UPI00003CBE2B UniRef100 entry UniRef100_UPI00003CBE2B 2416Hypothetical UPF0157 protein yqkA UniRef100_P54564 Bacillus subtilisYqkA [Bacillus subtilis] 2417 Hypothetical protein yqjZ [BacillusUniRef100_P54563 Bacillus subtilis YqjZ subtilis] 2418 Lipase precursor[Bacillus subtilis] UniRef100_P37957 Bacillus subtilis Lip 2419 2420Nickel ABC transporter [Bacillus UniRef100_Q9KBX8 Bacillus AppAhalodurans] halodurans 2421 Nickel ABC transporter [BacillusUniRef100_Q9KBX7 Bacillus AppB halodurans] halodurans 2422 Nickel ABCtransporter [Bacillus UniRef100_Q9KBX6 Bacillus AppC halodurans]halodurans 2423 Putative oligopeptide ABC transporter UniRef100_Q895A4Clostridium tetani DppD [Clostridium tetani] 2424 Putative oligopeptideABC transporter UniRef100_Q895A5 Clostridium tetani OppF [Clostridiumtetani] 2425 DNA-damage-inducible protein [Bacillus UniRef100_Q9KDR1Bacillus YojI halodurans] halodurans 2426 Fibronectin-binding protein[Bacillus UniRef100_Q63CW1 Bacillus cereus ZK cereus ZK] 2427Hypothetical protein yolD UniRef100_O64030 Bacteriophage YolD[Bacteriophage SPBc2] SPBc2 2428 UvrX 2429 Hypothetical protein yqzH[Bacillus UniRef100_O32014 Bacillus subtilis subtilis] 2430 Hypotheticaltransport protein yqjV UniRef100_P54559 Bacillus subtilis YqjV [Bacillussubtilis] 2431 Hypothetical protein yqjT [Bacillus UniRef100_P54557Bacillus subtilis YqjT subtilis] 2432 CoaA 2433 LacG [Lactococcuslactis] UniRef100_Q9RAU9 Lactococcus lactis 2434 LacF [Lactococcuslactis] UniRef100_Q9RAV2 Lactococcus YdbJ lactis 2435 2436 Hypotheticalprotein [Bacillus UniRef100_Q70K07 Bacillus amyloliquefaciensamyloliquefaciens] 2437 Hypothetical protein [Bacillus cereus]UniRef100_Q737G3 Bacillus cereus 2438 YmaC protein [Bacillus subtilis]UniRef100_O31789 Bacillus subtilis YmaC 2439 Hypothetical oxidoreductaseyqjQ UniRef100_P54554 Bacillus subtilis YqjQ [Bacillus subtilis] 2440YqjP 2441 ProI 2442 Hypothetical protein yqjN [Bacillus UniRef100_P54551Bacillus subtilis YqjN subtilis] 2443 Probable NADH-dependent flavinUniRef100_P54550 Bacillus subtilis YqjM oxidoreductase yqjM [Bacillussubtilis] 2444 Hypothetical protein yqjL [Bacillus UniRef100_P54549Bacillus subtilis YqjL subtilis] 2445 2446 Ribonuclease Z [Bacillussubtilis] UniRef100_P54548 Bacillus subtilis YqjK 2447Glucose-6-phosphate 1-dehydrogenase UniRef100_P54547 Bacillus subtilisZwf [Bacillus subtilis] 2448 Hypothetical conserved proteinUniRef100_Q8ELZ9 Oceanobacillus CitH [Oceanobacillus iheyensis]iheyensis 2449 Hypothetical protein OB3065 UniRef100_Q8ELZ8Oceanobacillus iheyensis [Oceanobacillus iheyensis] 2450 Immunogenicprotein [Oceanobacillus UniRef100_Q8ELZ7 Oceanobacillus SsuA iheyensis]iheyensis 2451 YdhQ protein [Bacillus subtilis] UniRef100_O05509Bacillus subtilis YdhQ 2452 Beta-glucosidase [Bacillus halodurans]UniRef100_Q9K615 Bacillus YdhP halodurans 2453 Putativecellobiose-specific enzyme IIC UniRef100_Q8KP28 Bacillus pumilus YdhO[Bacillus pumilus] 2454 YdhO protein [Bacillus subtilis]UniRef100_O05507 Bacillus subtilis YdhO 2455 YdhN protein [Bacillussubtilis] UniRef100_O05506 Bacillus subtilis YdhN 2456 PTS system,cellobiose-specific UniRef100_Q9K613 Bacillus halodurans enzyme II, Bcomponent [Bacillus halodurans] 2457 Alkaline phosphatase [Bacilluscereus UniRef100_Q639W1 Bacillus cereus PhoB ZK] ZK 2458 Glucose1-dehydrogenase A [Bacillus UniRef100_P10528 Bacillus Gdh megaterium]megaterium 2459 6-phosphogluconate dehydrogenase, UniRef100_P80859Bacillus subtilis YqjI decarboxylating II [Bacillus subtilis] 2460 DNApolymerase IV 1 [Bacillus subtilis] UniRef100_P54545 Bacillus subtilisYqjH 2461 Hypothetical protein yqzJ [Bacillus UniRef100_Q7WY64 Bacillussubtilis subtilis] 2462 YqjG 2463 Hypothetical protein yqjE [BacillusUniRef100_P54542 Bacillus subtilis YqjE subtilis] 2464 Methylmalonyl-CoAdecarboxylase UniRef100_Q9K8P6 Bacillus YqjD alpha subunit [Bacillushalodurans] halodurans 2465 Hypothetical protein yqjA [BacillusUniRef100_P54538 Bacillus subtilis YqjA subtilis] 2466 Probableamino-acid ABC transporter UniRef100_P54537 Bacillus subtilis YqiZATP-binding protein yqiZ [Bacillus subtilis] 2467 Probable amino-acidABC transporter UniRef100_P54536 Bacillus subtilis YqiY permease proteinyqiY [Bacillus subtilis] 2468 Probable amino-acid ABC transporterUniRef100_P54535 Bacillus subtilis YqiX extracellular binding proteinyqiX precursor [Bacillus subtilis] 2469 Hypothetical protein yqiW[Bacillus UniRef100_P54534 Bacillus subtilis YqiW subtilis] 2470 ProteinbmrU [Bacillus subtilis] UniRef100_P39074 Bacillus subtilis BmrU 2471Lipoamide acyltransferase component UniRef100_P37942 Bacillus subtilisBkdB of branched-chain alpha-keto acid dehydrogenase complex (EC2.3.1.168) (Dihydrolipoyllysine-residue (2- methylpropanoyl)transferase)[Bacillus subtilis] 2472 2-oxoisovalerate dehydrogenase betaUniRef100_P37941 Bacillus subtilis BkdAB subunit [Bacillus subtilis]2473 2-oxoisovalerate dehydrogenase alpha UniRef100_P37940 Bacillussubtilis BkdAA subunit [Bacillus subtilis] 2474 Dihydrolipoyldehydrogenase [Bacillus UniRef100_P54533 Bacillus subtilis LpdVsubtilis] 2475 Probable butyrate kinase [Bacillus UniRef100_P54532Bacillus subtilis Buk subtilis] 2476 Leucine dehydrogenase [BacillusUniRef100_P54531 Bacillus subtilis Bcd subtilis] 2477 Probable phosphatebutyryltransferase UniRef100_P54530 Bacillus subtilis Ptb [Bacillussubtilis] 2478 Putative sigma L-dependent UniRef100_P54529 Bacillussubtilis BkdR transcriptional regulator yqiR [Bacillus subtilis] 2479Hypothetical protein yqzF [Bacillus UniRef100_O32015 Bacillus subtilissubtilis] 2480 Hypothetical protein yqiK [Bacillus UniRef100_P54527Bacillus subtilis YqiK subtilis] 2481 Hypothetical protein yqiI[Bacillus UniRef100_P54525 Bacillus subtilis YqiI subtilis] 2482 YqiH2483 Serine O-acetyltransferase UniRef100_Q8PSY4 Methanosarcina CysE[Methanosarcina mazei] mazei 2484 2485 Stage 0 sporulation protein A[Bacillus UniRef100_P06534 Bacillus subtilis Spo0A subtilis] 2486 SpoIVB2487 DNA repair protein recN [Bacillus UniRef100_Q659H4 Bacillus RecNamyloliquefaciens] amyloliquefaciens 2488 Arginine repressor [Bacillussubtilis] UniRef100_P17893 Bacillus subtilis AhrC 2489 Hypotheticalprotein yqxC [Bacillus UniRef100_P19672 Bacillus subtilis YqxC subtilis]2490 Dxs 2491 Geranyltranstransferase [Bacillus UniRef100_P54383Bacillus subtilis YqiD subtilis] 2492 Probable exodeoxyribonuclease VIIUniRef100_P54522 Bacillus subtilis small subunit [Bacillus subtilis]2493 Probable exodeoxyribonuclease VII UniRef100_P54521 Bacillussubtilis YqiB large subunit [Bacillus subtilis] 2494 FolD bifunctionalprotein [Includes: UniRef100_P54382 Includes: FolDMethylenetetrahydrofolate Methylenetetrahydrofolate dehydrogenase (EC1.5.1.5); dehydrogenase Methenyltetrahydrofolate (EC 1.5.1.5);cyclohydrolase (EC 3.5.4.9)] [Bacillus Methenyltetrahydrofolatesubtilis] cyclohydrolase (EC 3.5.4.9) 2495 N utilization substanceprotein B UniRef100_P54520 Bacillus subtilis NusB homolog [Bacillussubtilis] 2496 Hypothetical protein yqhY [Bacillus UniRef100_P54519Bacillus subtilis YqhY subtilis] 2497 AccC 2498 Biotin carboxyl carrierprotein of acetyl- UniRef100_P49786 Bacillus subtilis AccB CoAcarboxylase [Bacillus subtilis] 2499 Stage III sporulation protein AHUniRef100_P49785 Bacillus subtilis SpoIIIAH [Bacillus subtilis] 2500Stage III sporulation protein AG UniRef100_P49784 Bacillus subtilisSpoIIIAG [Bacillus subtilis] 2501 Stage III sporulation protein AFUniRef100_P49783 Bacillus subtilis SpoIIIAF [Bacillus subtilis] 2502Stage III sporulation protein AE UniRef100_P49782 Bacillus subtilisSpoIIIAE [Bacillus subtilis] 2503 Stage III sporulation protein ADUniRef100_P49781 Bacillus subtilis SpoIIIAD [Bacillus subtilis] 2504Stage III sporulation protein AC UniRef100_P49780 Bacillus subtilis[Bacillus subtilis] 2505 Stage III sporulation protein ABUniRef100_Q01368 Bacillus subtilis SpoIIIAB [Bacillus subtilis] 2506SpoIIIAA 2507 Hypothetical protein yqhV [Bacillus UniRef100_P49779Bacillus subtilis subtilis] 2508 Elongation factor P [Bacillus subtilis]UniRef100_P49778 Bacillus subtilis Efp 2509 Putative peptidase yqhT[Bacillus UniRef100_P54518 Bacillus subtilis YqhT subtilis] 25103-dehydroquinate dehydratase [Bacillus UniRef100_P54517 Bacillussubtilis YqhS subtilis] 2511 Hypothetical protein yqhR [BacillusUniRef100_P54516 Bacillus subtilis YqhR subtilis] 2512 Hypotheticalprotein yqhQ [Bacillus UniRef100_P54515 Bacillus subtilis YqhQ subtilis]2513 Hypothetical protein yqhP [Bacillus UniRef100_P54514 Bacillussubtilis YqhP subtilis] 2514 Hypothetical protein yqhO [BacillusUniRef100_P54513 Bacillus subtilis YqhO subtilis] 2515 Transcriptionalregulator mntR [Bacillus UniRef100_P54512 Bacillus subtilis MntRsubtilis] 2516 Hypothetical protein yqhM [Bacillus UniRef100_P54511Bacillus subtilis YqhM subtilis] 2517 Hypothetical protein yqhL[Bacillus UniRef100_P54510 Bacillus subtilis YqhL subtilis] 2518 Glycinebetaine-binding protein UniRef100_P46922 Bacillus subtilis OpuACprecursor [Bacillus subtilis] 2519 Glycine betaine transport systemUniRef100_P46921 Bacillus subtilis OpuAB permease protein opuAB[Bacillus subtilis] 2520 Glycine betaine transport ATP-bindingUniRef100_P46920 Bacillus subtilis OpuAA protein opuAA [Bacillussubtilis] 2521 Probable glycine dehydrogenase UniRef100_P54377decarboxylating GcvPB [decarboxylating] subunit 2 [Bacillus subtilis]2522 Probable glycine dehydrogenase UniRef100_P54376 decarboxylatingGcvPA [decarboxylating] subunit 1 [Bacillus subtilis] 2523Aminomethyltransferase [Bacillus UniRef100_P54378 Bacillus subtilis GcvTsubtilis] 2524 Hypothetical helicase yqhH [Bacillus UniRef100_P54509Bacillus subtilis YqhH subtilis] 2525 Hypothetical protein yqhG[Bacillus UniRef100_P54508 Bacillus subtilis YqhG subtilis] 2526 2527SinR 2528 Spore coat-associated protein N UniRef100_P54507 Bacillussubtilis TasA [Bacillus subtilis] 2529 Signal peptidase I W [Bacillussubtilis] UniRef100_P54506 Bacillus subtilis SipW 2530 Hypotheticalprotein yqxM [Bacillus UniRef100_P40949 Bacillus subtilis YqxM subtilis]2531 YqzG protein [Bacillus subtilis] UniRef100_O32019 Bacillus subtilisYqzG 2532 YqzE protein [Bacillus subtilis] UniRef100_O32020 Bacillussubtilis 2533 ComGG 2534 ComGF 2535 ComG operon protein 5 precursorUniRef100_P25957 Bacillus subtilis ComGE [Bacillus subtilis] 2536 ComGoperon protein 4 precursor UniRef100_P25956 Bacillus subtilis ComGD[Bacillus subtilis] 2537 ComG operon protein 3 precursorUniRef100_P25955 Bacillus subtilis [Bacillus subtilis] 2538 ComGB 2539ComG operon protein 1 [Bacillus UniRef100_P25953 Bacillus subtilis ComGAsubtilis] 2540 Hypothetical protein yqhA [Bacillus UniRef100_P54504Bacillus subtilis YqhA subtilis] 2541 Hypothetical protein yqgZ[Bacillus UniRef100_P54503 Bacillus subtilis YqgZ subtilis] 2542Hypothetical protein yqgY [Bacillus UniRef100_P54502 Bacillus subtilissubtilis] 2543 Hypothetical protein yqgX [Bacillus UniRef100_P54501Bacillus subtilis YqgX subtilis] 2544 Hypothetical protein yqgW[Bacillus UniRef100_P54500 Bacillus subtilis subtilis] 2545 Hypotheticalprotein [Staphylococcus UniRef100_Q8CSE8 Staphylococcus epidermidisepidermidis] 2546 YqgU 2547 Hypothetical protein yqgT [BacillusUniRef100_P54497 Bacillus subtilis YqgT subtilis] 2548Ferrichrome-binding protein precursor UniRef100_P37580 Bacillus subtilisFhuD [Bacillus subtilis] 2549 Hypothetical protein yqgS [BacillusUniRef100_P54496 Bacillus subtilis YqgS subtilis] 2550 Glucokinase[Bacillus subtilis] UniRef100_P54495 Bacillus subtilis GlcK 2551Hypothetical protein yqgQ [Bacillus UniRef100_P54494 Bacillus subtilissubtilis] 2552 Stage V sporulation protein AF UniRef100_Q8EQ08Oceanobacillus SpoVAF [Oceanobacillus iheyensis] iheyensis 2553Hypothetical protein yqgP [Bacillus UniRef100_P54493 Bacillus subtilisYqgP subtilis] 2554 Hypothetical protein yqgO [Bacillus UniRef100_P54492Bacillus subtilis subtilis] 2555 Hypothetical protein yqgN [BacillusUniRef100_P54491 Bacillus subtilis YqgN subtilis] 2556 2557 YqgM 2558Hypothetical protein yqgL [Bacillus UniRef100_P54489 Bacillus subtilisYqgL subtilis] 2559 YqzD 2560 YqzC protein [Bacillus subtilis]UniRef100_O32023 Bacillus subtilis YqzC 2561 Phosphate importATP-binding protein UniRef100_P46342 Bacillus subtilis PstBB pstB 1[Bacillus subtilis] 2562 Phosphate import ATP-binding proteinUniRef100_P46341 Bacillus subtilis PstBA pstB 2 [Bacillus subtilis] 2563Probable ABC transporter permease UniRef100_P46340 Bacillus subtilisPstA protein yqgI [Bacillus subtilis] 2564 Probable ABC transporterpermease UniRef100_P46339 Bacillus subtilis PstC protein yqgH [Bacillussubtilis] 2565 Probable ABC transporter binding UniRef100_P46338Bacillus subtilis PstS protein yqgG precursor [Bacillus subtilis] 2566Hypothetical protein yqgF [Bacillus UniRef100_P54488 Bacillus subtilisPbpA subtilis] 2567 Hypothetical protein yqgE [Bacillus UniRef100_P54487Bacillus subtilis YqgE subtilis] 2568 SodA 2569 Hypothetical proteinyqgC [Bacillus UniRef100_P54486 Bacillus subtilis YqgC subtilis] 2570Hypothetical protein yqgB [Bacillus UniRef100_P54485 Bacillus subtilisYqgB subtilis] 2571 Hypothetical protein yqfZ [Bacillus UniRef100_P54483Bacillus subtilis subtilis] 2572 4-hydroxy-3-methylbut-2-en-1-ylUniRef100_P54482 Bacillus subtilis YqfY diphosphate synthase (EC1.17.4.3) (1- hydroxy-2-methyl-2-(E)-butenyl 4- diphosphate synthase)[Bacillus subtilis] 2573 Hypothetical protein yqfX [BacillusUniRef100_P54481 Bacillus subtilis YqfX subtilis] 2574 Putativenucleotidase yqfW [Bacillus UniRef100_P54480 Bacillus subtilis YqfWsubtilis] 2575 Zinc-specific metalloregulatory protein UniRef100_P54479Bacillus subtilis Zur [Bacillus subtilis] 2576 Metal (Zinc) transportprotein [Listeria UniRef100_Q926D9 Listeria innocua YceA innocua] 2577YcdI 2578 Hypothetical protein yqfU [Bacillus UniRef100_P54478 Bacillussubtilis YqfU subtilis] 2579 2580 Probable endonuclease IV [BacillusUniRef100_P54476 Bacillus subtilis YqfS subtilis] 2581 Probable RNAhelicase yqfR [Bacillus UniRef100_P54475 Bacillus subtilis YqfRsubtilis] 2582 Hypothetical protein yqfQ [Bacillus UniRef100_P54474Bacillus subtilis YqfQ subtilis] 2583 4-hydroxy-3-methylbut-2-enylUniRef100_P54473 Bacillus subtilis YqfP diphosphate reductase [Bacillussubtilis] 2584 YqfO 2585 Hypothetical protein yqfN [BacillusUniRef100_P54471 Bacillus subtilis YqfN subtilis] 2586 YwqL protein[Bacillus subtilis] UniRef100_P96724 Bacillus subtilis YwqL 2587 25882589 2590 2591 Hypothetical protein CAC0336 UniRef100_Q97M62 Clostridiumacetobutylicum [Clostridium acetobutylicum] 2592 Hypothetical protein[Bacillus UniRef100_Q6HGF2 Bacillus thuringiensis thuringiensis] 25932594 YwqJ protein [Bacillus subtilis] UniRef100_P96722 Bacillus subtilisYwqJ 2595 Hypothetical protein ywqI [Bacillus UniRef100_P96721 Bacillussubtilis subtilis] 2596 YwqH 2597 Cytochrome c-550 [Bacillus subtilis]UniRef100_P24469 Bacillus subtilis CccA 2598 SigA 2599 DNA primase[Bacillus subtilis] UniRef100_P05096 Bacillus subtilis DnaG 2600Hypothetical UPF0178 protein yqxD UniRef100_P17868 Bacillus subtilisYqxD [Bacillus subtilis] 2601 Hypothetical UPF0085 protein yqfLUniRef100_P54470 Bacillus subtilis YqfL [Bacillus subtilis] 2602 YqzBprotein [Bacillus subtilis] UniRef100_O34994 Bacillus subtilis YqzB 2603Glycyl-tRNA synthetase beta chain UniRef100_P54381 Bacillus subtilisGlyS [Bacillus subtilis] 2604 Glycyl-tRNA synthetase alpha chainUniRef100_P54380 Bacillus subtilis GlyQ [Bacillus subtilis] 2605 DNArepair protein recO [Bacillus UniRef100_P42095 Bacillus subtilis RecOsubtilis] 2606 2607 GTP-binding protein era homolog UniRef100_P42182Bacillus subtilis Era [Bacillus subtilis] 2608 Cytidine deaminase[Bacillus subtilis] UniRef100_P19079 Bacillus subtilis Cdd 2609 2610Hypothetical UPF0054 protein yqfG UniRef100_P46347 Bacillus subtilisYqfG [Bacillus subtilis] 2611 Hypothetical protein yqfF [BacillusUniRef100_P46344 Bacillus subtilis YqfF subtilis] 2612 PhoH-like protein[Bacillus subtilis] UniRef100_P46343 Bacillus subtilis PhoH 2613 YqfD2614 Hypothetical protein yqfC [Bacillus UniRef100_P54468 Bacillussubtilis subtilis] 2615 Hypothetical protein yqfB [BacillusUniRef100_P54467 Bacillus subtilis YqfB subtilis] 2616 Hypotheticalprotein yqfA [Bacillus UniRef100_P54466 Bacillus subtilis YqfA subtilis]2617 YqeZ 2618 Hypothetical protein yqeY [Bacillus UniRef100_P54464Bacillus subtilis YqeY subtilis] 2619 2620 Hypothetical protein yqeW[Bacillus UniRef100_P54463 Bacillus subtilis YqeW subtilis] 2621Deoxyribose-phosphate aldolase UniRef100_Q92A19 Listeria innocua Dra[Listeria innocua] 2622 Hypothetical UPF0004 protein yqeVUniRef100_P54462 Bacillus subtilis YqeV [Bacillus subtilis] 2623Hypothetical UPF0088 protein yqeU UniRef100_P54461 Bacillus subtilisYqeU [Bacillus subtilis] 2624 Ribosomal protein L11 UniRef100_P54460Bacillus subtilis YqeT methyltransferase [Bacillus subtilis] 2625Chaperone protein dnaJ [Bacillus UniRef100_P17631 Bacillus subtilis DnaJsubtilis] 2626 DnaK 2627 2628 2629 Heat-inducible transcriptionrepressor UniRef100_P25499 Bacillus subtilis HrcA hrcA [Bacillussubtilis] 2630 Probable oxygen-independent UniRef100_P54304 Bacillussubtilis HemN coproporphyrinogen III oxidase [Bacillus subtilis] 2631LepA 2632 Hypothetical protein yqxA [Bacillus UniRef100_P38425 Bacillussubtilis YqxA subtilis] 2633 SpoIIP 2634 Germination protease precursorUniRef100_P22322 Bacillus subtilis Gpr [Bacillus subtilis] 2635 30Sribosomal protein S20 [Bacillus UniRef100_P21477 Bacillus subtilissubtilis] 2636 Hypothetical protein yqeN [Bacillus UniRef100_P54459Bacillus subtilis YqeN subtilis] 2637 2638 ComE operon protein 3[Bacillus UniRef100_P39695 Bacillus subtilis ComEC subtilis] 2639 ComEoperon protein 2 [Bacillus UniRef100_P32393 Bacillus subtilis ComEBsubtilis] 2640 ComE operon protein 1 [Bacillus UniRef100_P39694 Bacillussubtilis ComEA subtilis] 2641 ComE operon protein 4 [BacillusUniRef100_P39696 Bacillus subtilis ComER subtilis] 2642 Hypotheticalprotein yqeM [Bacillus UniRef100_P54458 Bacillus subtilis YqeM subtilis]2643 Hypothetical protein yqeL [Bacillus UniRef100_P54457 Bacillussubtilis YqeL subtilis] 2644 Hypothetical protein yqeK [BacillusUniRef100_P54456 Bacillus subtilis YqeK subtilis] 2645Nicotinate-nucleotide UniRef100_P54455 Bacillus subtilis YqeJadenylyltransferase (EC 2.7.7.18) (Deamido-NAD(+) pyrophosphorylase)(Deamido-NAD(+) diphosphorylase) [Bacillus subtilis] 2646 HypotheticalUPF0044 protein yqeI UniRef100_P54454 Bacillus subtilis [Bacillussubtilis] 2647 Shikimate dehydrogenase [Bacillus UniRef100_P54374Bacillus subtilis AroD subtilis] 2648 Hypothetical protein yqeH[Bacillus UniRef100_P54453 Bacillus subtilis YqeH subtilis] 2649Hypothetical protein yqeG [Bacillus UniRef100_P54452 Bacillus subtilisYqeG subtilis] 2650 2651 2652 Hypothetical lipoprotein yqeF precursorUniRef100_P54451 Bacillus subtilis YqeF [Bacillus subtilis] 2653Acetyltransferase, GNAT family UniRef100_Q81KW8 Bacillus YdfB [Bacillusanthracis] anthracis 2654 Hypothetical protein yrhF [BacillusUniRef100_O05398 Bacillus subtilis YrhF subtilis] 2655 Formatedehydrogenase chain A UniRef100_O05397 Bacillus subtilis YrhE [Bacillussubtilis] 2656 Hypothetical protein yrhD [Bacillus UniRef100_O05396Bacillus subtilis YrhD subtilis] 2657 2658 RNA polymerase sigma-K factorUniRef100_P12254 Bacillus subtilis SpoIIIC precursor [Bacillus subtilis]2659 YcnB 2660 BH2157 protein [Bacillus halodurans] UniRef100_Q9KAX9Bacillus YuaI halodurans 2661 2662 2663 Alanyl-tRNA synthetase familyprotein UniRef100_Q81Y73 Bacillus AlaS [Bacillus anthracis] anthracis2664 METAL-ACTIVATED PYRIDOXAL UniRef100_Q8YCI2 Brucella melitensisENZYME [Brucella melitensis] 2665 Probable translation initiationinhibitor UniRef100_Q6LKM3 Photobacterium YabJ [Photobacteriumprofundum)] profundum) 2666 YccC 2667 Putative threonine synthaseUniRef100_Q82IF6 Streptomyces ThrC [Streptomyces avermitilis]avermitilis 2668 YabJ 2669 Hypothetical protein UniRef100_Q8RBA0Thermoanaerobacter [Thermoanaerobacter tengcongensis] tengcongensis 26702671 UPI000032CE59 UniRef100 entry UniRef100_UPI000032CE59 2672Multidrug-efflux transporter 1 regulator UniRef100_P39075 Bacillussubtilis BmrR [Bacillus subtilis] 2673Metallo-beta-lactamase/rhodanese-like UniRef100_Q81Q95 Bacillus YrkHdomain protein [Bacillus anthracis] anthracis 2674 Hypothetical protein[Bacillus cereus UniRef100_Q63B51 Bacillus cereus YumB ZK] ZK 2675 NreC[Staphylococcus carnosus] UniRef100_Q7WZY4 Staphylococcus DegU carnosus2676 Two-component sensor histidine kinase UniRef100_Q67JE7Symbiobacterium DegS [Symbiobacterium thermophilum] thermophilum 2677YdfQ protein [Bacillus subtilis] UniRef100_P96695 Bacillus subtilis YdfQ2678 Hypothetical Membrane Spanning UniRef100_Q813Y5 Bacillus cereusYrkJ Protein [Bacillus cereus] 2679 Hypothetical UPF0033 protein yrkIUniRef100_P54436 Bacillus subtilis [Bacillus subtilis] 2680UPI00003CB3C6 UniRef100 entry UniRef100_UPI00003CB3C6 YrkH 2681Molybdopterin biosynthesis MoeB UniRef100_Q81HL2 Bacillus cereus YrkFprotein [Bacillus cereus] 2682 Hypothetical protein yrkE [BacillusUniRef100_P54432 Bacillus subtilis YrkE subtilis] 2683 Hypotheticalconserved protein UniRef100_Q8EN37 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 2684 S-adenosylmethionine-dependentUniRef100_Q97FB3 Clostridium YcgJ methyltransferase [Clostridiumacetobutylicum acetobutylicum] 2685 Hypothetical protein [Bacillusanthracis] UniRef100_Q81N81 Bacillus anthracis 2686 YciB protein[Bacillus subtilis] UniRef100_P94399 Bacillus subtilis 2687 Acetylxylanesterase related enzyme UniRef100_Q97LM8 Clostridium acetobutylicum[Clostridium acetobutylicum] 2688 Hypothetical UPF0161 proteinUniRef100_P61464 Bacillus cereus BCE4947 [Bacillus cereus] 2689Delta-aminolevulinic acid dehydratase UniRef100_Q9K8G2 Bacillushalodurans [Bacillus halodurans] 2690 6-phospho-3-hexuloisomerase[Bacillus UniRef100_Q6TV53 Bacillus HxlB methanolicus] methanolicus 2691Probable hexulose-6-phosphate UniRef100_P42405 Bacillus subtilis HxlAsynthase [Bacillus subtilis] 2692 Transcriptional regulator [BacillusUniRef100_Q70KJ9 Bacillus HxlR amyloliquefaciens] amyloliquefaciens 2693Fatty acid desaturase [Bacillus subtilis] UniRef100_O34653 Bacillussubtilis Des 2694 Sensor kinase [Bacillus subtilis] UniRef100_O34757Bacillus subtilis YocF 2695 Sensor regulator [Bacillus subtilis]UniRef100_O34723 Bacillus subtilis YocG 2696 UPI00003CC1E4 UniRef100entry UniRef100_UPI00003CC1E4 YcgT 2697 Nickel transport system[Bacillus UniRef100_Q9KFB8 Bacillus AppA halodurans] halodurans 2698Nickel transport system [Bacillus UniRef100_Q9KFB7 Bacillus AppBhalodurans] halodurans 2699 Nickel transport system [BacillusUniRef100_Q9KFB6 Bacillus AppC halodurans] halodurans 2700 OligopeptideABC transporter [Bacillus UniRef100_Q9KFB5 Bacillus DppD halodurans]halodurans 2701 Oligopeptide ABC transporter [Bacillus UniRef100_Q9KFB4Bacillus AppF halodurans] halodurans 2702 UPI00003CB880 UniRef100 entryUniRef100_UPI00003CB880 YdfL 2703 UPI00003CA374 UniRef100 entryUniRef100_UPI00003CA374 YoeA 2704 PROBABLE TRANSCRIPTIONUniRef100_Q8XS91 Ralstonia solanacearum REGULATOR PROTEIN [Ralstoniasolanacearum] 2705 Short chain dehydrogenase family UniRef100_Q834I5Enterococcus YvaG protein [Enterococcus faecalis] faecalis 2706Uncharacterized protein, containing UniRef100_Q97L24 Clostridiumacetobutylicum predicted phosphatase domain [Clostridium acetobutylicum]2707 UPI000025758C UniRef100 entry UniRef100_UPI000025758C PnbA 2708Cytochrome P450 [Bacillus subtilis] UniRef100_O08469 Bacillus subtilisCypA 2709 YtnM [Bacillus subtilis] UniRef100_O34430 Bacillus subtilisYtnM 2710 Hypothetical protein yndA precursor UniRef100_O31805 Bacillussubtilis YndA [Bacillus subtilis] 2711 YvaG protein [Bacillus subtilis]UniRef100_O32229 Bacillus subtilis YvaG 2712 Levanase precursor[Bacillus subtilis] UniRef100_P05656 Bacillus subtilis SacC 2713 PTSsystem, fructose-specific IID UniRef100_P26382 Bacillus subtilis LevGcomponent [Bacillus subtilis] 2714 PTS system, fructose-specific IICUniRef100_P26381 Bacillus subtilis LevF component [Bacillus subtilis]2715 PTS system, fructose-specific IIB UniRef100_P26380 Bacillussubtilis LevE component [Bacillus subtilis] 2716 PTS system,fructose-specific IIA UniRef100_P26379 Bacillus subtilis LevD component[Bacillus subtilis] 2717 Transcriptional regulatory protein levRUniRef100_P23914 Bacillus subtilis LevR [Bacillus subtilis] 2718Hypothetical protein [Bacillus cereus] UniRef100_Q734Q5 Bacillus cereus2719 Hypothetical protein yrhK [Bacillus UniRef100_O05401 Bacillussubtilis subtilis] 2720 UPI00003CB785 UniRef100 entryUniRef100_UPI00003CB785 2721 AdaA 2722 Methylated-DNA--protein-cysteineS- UniRef100_Q732Y7 Bacillus cereus AdaB methyltransferase [Bacilluscereus] 2723 Oxidoreductase, aldo/keto reductase UniRef100_Q6HBJ5Bacillus YtbE family [Bacillus thuringiensis] thuringiensis 2724 YtbD2725 Hypothetical UPF0087 protein ytcD UniRef100_O34533 Bacillussubtilis YtcD [Bacillus subtilis] 2726 2727 Hypothetical protein yjjA[Bacillus UniRef100_O34394 Bacillus subtilis YjjA subtilis] 2728UPI000028298B UniRef100 entry UniRef100_UPI000028298B 2729 YxeB 2730Putative HTH-type transcriptional UniRef100_P37499 Bacillus subtilisYybE regulator yybE [Bacillus subtilis] 2731 Hypothetical transportprotein yybF UniRef100_P37498 Bacillus subtilis YybF [Bacillus subtilis]2732 Hypothetical protein [Bacillus cereus] UniRef100_Q732J0 Bacilluscereus 2733 Probable bifunctional P-450:NADPH- UniRef100_O08336Includes: YrhJ P450 reductase 2 [Includes: Cytochrome Cytochrome P450102 (EC 1.14.14.1); P450 102 (EC NADPH--cytochrome P450 reductase1.14.14.1); (EC 1.6.2.4)] [Bacillus subtilis] NADPH-- cytochrome P450reductase (EC 1.6.2.4) 2734 Regulatory protein [Bacillus subtilis]UniRef100_O08335 Bacillus subtilis YrhI 2735 WprA 2736 YrhH [Bacillussubtilis] UniRef100_O05400 Bacillus subtilis YrhH 2737 2738 2739 2740Cystathionine gamma-lyase [Bacillus UniRef100_O05394 Bacillus subtilisYrhB subtilis] 2741 Cysteine synthase [Bacillus subtilis]UniRef100_O05393 Bacillus subtilis YrhA 2742 MTA/SAH nucleosidase[Bacillus UniRef100_O32028 Bacillus subtilis Mtn subtilis] 2743 YrrTprotein [Bacillus subtilis] UniRef100_O32029 Bacillus subtilis YrrT 2744Hypothetical protein yrzA [Bacillus UniRef100_O32030 Bacillus subtilissubtilis] 2745 YrrS 2746 YrrR protein [Bacillus subtilis]UniRef100_O32032 Bacillus subtilis YrrR 2747 Transcription elongationfactor greA UniRef100_P80240 Bacillus subtilis GreA [Bacillus subtilis]2748 Uridine kinase [Bacillus subtilis] UniRef100_O32033 Bacillussubtilis Udk 2749 YrrO protein [Bacillus subtilis] UniRef100_O32034Bacillus subtilis YrrO 2750 YrrN protein [Bacillus subtilis]UniRef100_O32035 Bacillus subtilis YrrN 2751 YrrM protein [Bacillussubtilis] UniRef100_O32036 Bacillus subtilis YrrM 2752 YrrL protein[Bacillus subtilis] UniRef100_O34758 Bacillus subtilis YrrL 2753 YrzBprotein [Bacillus subtilis] UniRef100_O34828 Bacillus subtilis 2754Putative Holliday junction resolvase UniRef100_O34634 Bacillus subtilisYrrK [Bacillus subtilis] 2755 Hypothetical UPF0297 protein yrzLUniRef100_Q7WY61 Bacillus subtilis [Bacillus subtilis] 2756 Alanyl-tRNAsynthetase [Bacillus UniRef100_O34526 Bacillus subtilis AlaS subtilis]2757 Hypothetical UPF0118 protein yrrI UniRef100_O34472 Bacillussubtilis YrrI [Bacillus subtilis] 2758 2759 Hypothetical protein[Bacillus cereus UniRef100_Q634F2 Bacillus cereus ZK ZK] 2760 YrrDprotein [Bacillus subtilis] UniRef100_O34402 Bacillus subtilis YrrD 2761YrrC protein [Bacillus subtilis] UniRef100_O34481 Bacillus subtilis YrrC2762 YrrB protein [Bacillus subtilis] UniRef100_O34452 Bacillus subtilisYrrB 2763 Probable tRNA (5-methylaminomethyl- UniRef100_O35020 Bacillussubtilis TrmU 2-thiouridylate)-methyltransferase [Bacillus subtilis]2764 YrvO protein [Bacillus subtilis] UniRef100_O34599 Bacillus subtilisYrvO 2765 BH1259 protein [Bacillus halodurans] UniRef100_Q9KDF4 Bacillushalodurans 2766 YrvN protein [Bacillus subtilis] UniRef100_O34528Bacillus subtilis YrvN 2767 YrvM protein [Bacillus subtilis]UniRef100_O32037 Bacillus subtilis YrvM 2768 Aspartyl-tRNA synthetase[Bacillus UniRef100_O32038 Bacillus subtilis AspS subtilis] 2769Histidyl-tRNA synthetase [Bacillus UniRef100_O32039 Bacillus subtilisHisS subtilis] 2770 2771 YrvJ 2772 Putative D-tyrosyl-tRNA(Tyr)UniRef100_O32042 Bacillus subtilis YrvI deacylase-like protein [Bacillussubtilis] 2773 GTP pyrophosphokinase (EC 2.7.6.5) UniRef100_O54408Bacillus subtilis RelA (ATP:GTP 3′-pyrophosphotransferase) (ppGppsynthetase I) ((P)ppGpp synthetase) [Bacillus subtilis] 2774 Adeninephosphoribosyltransferase UniRef100_O34443 Bacillus subtilis Apt[Bacillus subtilis] 2775 YrvE protein [Bacillus subtilis]UniRef100_O32044 Bacillus subtilis YrvE 2776 YrvD protein [Bacillussubtilis] UniRef100_O32045 Bacillus subtilis 2777 SecDF protein[Bacillus subtilis] UniRef100_O32047 Bacillus subtilis SecDF 2778 YrzDprotein [Bacillus subtilis] UniRef100_O32049 Bacillus subtilis 2779SpoVB 2780 Hypothetical protein yrbG [Bacillus UniRef100_O32050 Bacillussubtilis YrbG subtilis] 2781 YrzE protein [Bacillus subtilis]UniRef100_O32051 Bacillus subtilis YrzE 2782 Hypothetical UPF0092protein yrbF UniRef100_O32052 Bacillus subtilis [Bacillus subtilis] 2783Queuine tRNA-ribosyltransferase UniRef100_O32053 Bacillus subtilis Tgt[Bacillus subtilis] 2784 S-adenosylmethionine:tRNA UniRef100_O32054Bacillus subtilis QueA ribosyltransferase-isomerase [Bacillus subtilis]2785 2786 Holliday junction DNA helicase ruvB UniRef100_O32055 Bacillussubtilis RuvB [Bacillus subtilis] 2787 Holliday junction DNA helicaseruvA UniRef100_O05392 Bacillus subtilis RuvA [Bacillus subtilis] 2788BofC protein precursor [Bacillus subtilis] UniRef100_O05391 Bacillussubtilis BofC 2789 Hypothetical conserved protein UniRef100_Q8ERL7Oceanobacillus YrzF [Oceanobacillus iheyensis] iheyensis 2790 2791Small, acid-soluble spore protein H UniRef100_Q9KB75 Bacillus halodurans[Bacillus halodurans] 2792 Hypothetical protein yjoA [BacillusUniRef100_O34334 Bacillus subtilis YjoA subtilis] 2793 YmaC protein[Bacillus subtilis] UniRef100_O31789 Bacillus subtilis YmaC 2794 27952796 Hypothetical UPF0082 protein yrbC UniRef100_P94447 Bacillussubtilis YrbC [Bacillus subtilis] 2797 Sporulation cortex protein coxAUniRef100_P94446 Bacillus subtilis CoxA [Bacillus subtilis] 2798Morphogenetic protein associated with UniRef100_O32062 Bacillus subtilisSafA SpoVID [Bacillus subtilis] 2799 Quinolinate synthetase A [BacillusUniRef100_Q9KWZ1 Bacillus subtilis NadA subtilis] 2800 Probablenicotinate-nucleotide UniRef100_P39666 carboxylating NadCpyrophosphorylase [carboxylating] [Bacillus subtilis] 2801 L-aspartateoxidase [Bacillus subtilis] UniRef100_P38032 Bacillus subtilis NadB 2802Probable cysteine desulfurase [Bacillus UniRef100_P38033 Bacillussubtilis NifS subtilis] 2803 YrxA 2804 Prephenate dehydratase [BacillusUniRef100_P21203 Bacillus subtilis PheA subtilis] 2805 ACT domainprotein pheB [Bacillus UniRef100_P21204 Bacillus subtilis PheB subtilis]2806 Spo0B-associated GTP-binding protein UniRef100_Q659J4 Bacillus Obg[Bacillus amyloliquefaciens] amyloliquefaciens 2807 Sporulationinitiation UniRef100_P06535 Bacillus subtilis Spo0B phosphotransferase B[Bacillus subtilis] 2808 50S ribosomal protein L27 [BacillusUniRef100_P05657 Bacillus subtilis subtilis] 2809 2810 50S ribosomalprotein L21 [Bacillus UniRef100_P26908 Bacillus subtilis RplU subtilis]2811 Stage IV sporulation protein FB UniRef100_P26937 Bacillus subtilisSpoIVFB [Bacillus subtilis] 2812 Stage IV sporulation protein FAUniRef100_P26936 Bacillus subtilis SpoIVFA [Bacillus subtilis] 2813Hypothetical protein [Bacillus cereus] UniRef100_Q816V6 Bacillus cereusYndB 2814 Transcriptional regulator, ArsR family UniRef100_Q632Y0Bacillus cereus ZK [Bacillus cereus ZK] 2815 Septum site-determiningprotein minD UniRef100_Q01464 Bacillus subtilis MinD [Bacillus subtilis]2816 MinC 2817 Rod shape-determining protein mreD UniRef100_Q01467Bacillus subtilis MreD [Bacillus subtilis] 2818 Rod shape-determiningprotein mreC UniRef100_Q01466 Bacillus subtilis MreC [Bacillus subtilis]2819 Rod shape-determining protein mreB UniRef100_Q01465 Bacillussubtilis MreB [Bacillus subtilis] 2820 DNA repair protein radC homologUniRef100_Q02170 Bacillus subtilis RadC [Bacillus subtilis] 2821 Septumformation protein Maf [Bacillus UniRef100_Q02169 Bacillus subtilis Mafsubtilis] 2822 Stage II sporulation protein B [Bacillus UniRef100_P37575Bacillus subtilis SpoIIB subtilis] 2823 Type 4 prepilin-like proteinsleader UniRef100_P15378 Includes: Leader ComC peptide processing enzyme(Late peptidase (EC competence protein comC) [Includes: 3.4.23.43)Leader peptidase (EC 3.4.23.43) (Prepilin (Prepilin peptidase); N-peptidase); N- methyltransferase (EC 2.1.1.—)] [Bacillusmethyltransferase subtilis] (EC 2.1.1.—) 2824 FolC 2825 Valyl-tRNAsynthetase [Bacillus subtilis] UniRef100_Q05873 Bacillus subtilis ValS2826 Hypothetical protein OB2062 UniRef100_Q8EPN1 Oceanobacillusiheyensis [Oceanobacillus iheyensis] 2827 Hypothetical protein ysxE[Bacillus UniRef100_P37964 Bacillus subtilis YsxE subtilis] 2828 StageVI sporulation protein D [Bacillus UniRef100_P37963 Bacillus subtilisSpoVID subtilis] 2829 Glutamate-1-semialdehyde 2,1- UniRef100_P30949Bacillus subtilis HemL aminomutase [Bacillus subtilis] 2830Delta-aminolevulinic acid dehydratase UniRef100_P30950 Bacillus subtilisHemB [Bacillus subtilis] 2831 Uroporphyrinogen-III synthase [BacillusUniRef100_P21248 Bacillus subtilis HemD subtilis] 2832 Porphobilinogendeaminase [Bacillus UniRef100_P16616 Bacillus subtilis HemC subtilis]2833 Protein hemX [Bacillus subtilis] UniRef100_P16645 Bacillus subtilisHemX 2834 Glutamyl-tRNA reductase [Bacillus UniRef100_P16618 Bacillussubtilis HemA subtilis] 2835 Hypothetical protein ysxD [BacillusUniRef100_P40736 Bacillus subtilis YsxD subtilis] 2836 ProbableGTP-binding protein engB UniRef100_P38424 Bacillus subtilis YsxC[Bacillus subtilis] 2837 ATP-dependent protease La 1 [BacillusUniRef100_P37945 Bacillus subtilis LonA subtilis] 2838 ATP-dependentprotease La homolog UniRef100_P42425 Bacillus subtilis LonB [Bacillussubtilis] 2839 ATP-dependent Clp protease ATP- UniRef100_P50866 Bacillussubtilis ClpX binding subunit clpX [Bacillus subtilis] 2840 Triggerfactor [Bacillus subtilis] UniRef100_P80698 Bacillus subtilis Tig 2841Hypothetical protein ysoA [Bacillus UniRef100_P94569 Bacillus subtilisYsoA subtilis] 2842 3-isopropylmalate dehydratase small UniRef100_P94568Bacillus subtilis LeuD subunit [Bacillus subtilis] 28433-isopropylmalate dehydratase large UniRef100_P80858 Bacillus subtilisLeuC subunit [Bacillus subtilis] 2844 LeuB 2845 2-isopropylmalatesynthase [Bacillus UniRef100_P94565 Bacillus subtilis LeuA subtilis]2846 Ketol-acid reductoisomerase [Bacillus UniRef100_P37253 Bacillussubtilis IlvC subtilis] 2847 Acetolactate synthase small subunitUniRef100_P37252 Bacillus subtilis IlvH [Bacillus subtilis] 2848Acetolactate synthase large subunit UniRef100_P37251 Bacillus subtilisIlvB [Bacillus subtilis] 2849 Branched-chain amino acid UniRef100_Q6HLF7Bacillus Dat aminotransferase [Bacillus thuringiensis thuringiensis]2850 2851 RocG 2852 BH3337 protein [Bacillus halodurans]UniRef100_Q9K7M4 Bacillus halodurans 2853 YxeD 2854 Hypothetical proteinyqbA [Bacillus UniRef100_P45917 Bacillus subtilis subtilis] 2855Hypothetical protein yqaT [Bacillus UniRef100_P45916 Bacillus subtilisYqaT subtilis] 2856 Lin1266 protein [Listeria innocua] UniRef100_Q92CC3Listeria innocua 2857 Lin1733 protein [Listeria innocua]UniRef100_Q92B18 Listeria innocua 2858 2859 2860 2861 MtbP 2862 BH3535protein [Bacillus halodurans] UniRef100_Q9K738 Bacillus halodurans 2863YeeF 2864 YxiD 2865 RapI 2866 2867 2868 2869 Hypothetical UPF0025protein ysnB UniRef100_P94559 Bacillus subtilis YsnB [Bacillus subtilis]2870 HAM1 protein homolog [Bacillus UniRef100_P94558 Bacillus subtilisYsnA subtilis] 2871 Rph 2872 Germination protein gerM [BacillusUniRef100_P39072 Bacillus subtilis GerM subtilis] 2873 RacE 2874Hypothetical protein ysmB [Bacillus UniRef100_P97247 Bacillus subtilisYsmB subtilis] 2875 Germination protein gerE [Bacillus UniRef100_P11470Bacillus subtilis subtilis] 2876 Oxidoreductase [ClostridiumUniRef100_Q97TP7 Clostridium YqjQ acetobutylicum] acetobutylicum 2877Hypothetical protein ysmA [Bacillus UniRef100_Q6L874 Bacillus subtilisYsmA subtilis] 2878 Succinate dehydrogenase iron-sulfur UniRef100_P08066Bacillus subtilis SdhB protein [Bacillus subtilis] 2879 SdhA 2880 2881Succinate dehydrogenase cytochrome UniRef100_P08064 Bacillus subtilisSdhC B-558 subunit [Bacillus subtilis] 2882 Hypothetical protein yslB[Bacillus UniRef100_P42955 Bacillus subtilis YslB subtilis] 2883Aspartokinase 2 (EC 2.7.2.4) UniRef100_P08495 Contains: LysC(Aspartokinase II) (Aspartate kinase 2) Aspartokinase II [Contains:Aspartokinase II alpha alpha subunit; subunit; Aspartokinase II betasubunit] Aspartokinase II [Bacillus subtilis] beta subunit 2884 UvrABCsystem protein C [Bacillus UniRef100_P14951 Bacillus subtilis UvrCsubtilis] 2885 Thioredoxin [Bacillus subtilis] UniRef100_P14949 Bacillussubtilis TrxA 2886 Electron transfer flavoprotein alpha-UniRef100_P94551 Bacillus subtilis EtfA subunit [Bacillus subtilis] 2887Electron transfer flavoprotein beta- UniRef100_P94550 Bacillus subtilisEtfB subunit [Bacillus subtilis] 2888 Hypothetical protein ysiB[Bacillus UniRef100_P94549 Bacillus subtilis YsiB subtilis] 2889Hypothetical protein ysiA [Bacillus UniRef100_P94548 Bacillus subtilisYsiA subtilis] 2890 Long-chain-fatty-acid--CoA ligase UniRef100_P94547Bacillus subtilis LcfA [Bacillus subtilis] 2891 Hypothetical proteinyshE [Bacillus UniRef100_P94546 Bacillus subtilis YshE subtilis] 2892MutS2 protein [Bacillus subtilis] UniRef100_P94545 Bacillus subtilisMutSB 2893 Hypothetical protein yshC [Bacillus UniRef100_P94544 Bacillussubtilis YshC subtilis] 2894 YshB 2895 2896 Ribonuclease HIII [Bacillussubtilis] UniRef100_P94541 Bacillus subtilis RnhC 2897 2898 2899 29002901 YxlF 2902 2903 Phenylalanyl-tRNA synthetase beta UniRef100_P17922Bacillus subtilis PheT chain [Bacillus subtilis] 2904 Phenylalanyl-tRNAsynthetase alpha UniRef100_Q659J3 Bacillus PheS chain [Bacillusamyloliquefaciens] amyloliquefaciens 2905 Hypothetical protein ysgA[Bacillus UniRef100_P94538 Bacillus subtilis YsgA subtilis] 2906 Small,acid-soluble spore protein I UniRef100_P94537 Bacillus subtilis[Bacillus subtilis] 2907 Carbon starvation protein A homologUniRef100_P94532 Bacillus subtilis CstA [Bacillus subtilis] 2908Alpha-N-arabinofuranosidase [Bacillus UniRef100_Q9XBQ3 Bacillus AbfAstearothermophilus] stearothermophilus 2909 L-arabinose transport systempermease UniRef100_P94530 Bacillus subtilis AraQ protein araQ [Bacillussubtilis] 2910 L-arabinose transport system permease UniRef100_P94529Bacillus subtilis AraP protein araP [Bacillus subtilis] 2911 Probablearabinose-binding protein UniRef100_P94528 Bacillus subtilis AraNprecursor [Bacillus subtilis] 2912 Arabinose operon protein araMUniRef100_P94527 Bacillus subtilis AraM [Bacillus subtilis] 2913L-ribulose-5-phosphate 4-epimerase UniRef100_P94525 Bacillus subtilisAraD [Bacillus subtilis] 2914 Ribulokinase [Bacillus subtilis]UniRef100_P94524 Bacillus subtilis AraB 2915 L-arabinose isomerase[Bacillus UniRef100_P94523 Bacillus subtilis AraA subtilis] 2916 AbnA2917 Hypothetical protein ysdC [Bacillus UniRef100_P94521 Bacillussubtilis YsdC subtilis] 2918 Hypothetical protein ysdB [BacillusUniRef100_P94520 Bacillus subtilis YsdB subtilis] 2919 Hypotheticalprotein ysdA [Bacillus UniRef100_P94519 Bacillus subtilis subtilis] 292050S ribosomal protein L20 [Bacillus UniRef100_P55873 Bacillus subtilisRplT subtilis] 2921 50S ribosomal protein L35 [Bacillus UniRef100_P55874Bacillus subtilis subtilis] 2922 InfC 2923 Antiholin-like protein IrgB[Bacillus UniRef100_P94516 Bacillus subtilis YsbB subtilis] 2924Antiholin-like protein IrgA [Bacillus UniRef100_P94515 Bacillus subtilisYsbA subtilis] 2925 PhoD 2926 Sensory transduction protein lytTUniRef100_P94514 Bacillus subtilis LytT [Bacillus subtilis] 2927 Sensorprotein lytS [Bacillus subtilis] UniRef100_P94513 Bacillus subtilis LytS2928 Hypothetical protein [Bacillus anthracis] UniRef100_Q81N00 Bacillusanthracis 2929 Hypothetical protein ysaA [Bacillus UniRef100_P94512Bacillus subtilis YsaA subtilis] 2930 Threonyl-tRNA synthetase 1[Bacillus UniRef100_P18255 Bacillus subtilis ThrS subtilis] 2931Hypothetical protein ytxC [Bacillus UniRef100_P06569 Bacillus subtilisYtxC subtilis] 2932 Hypothetical UPF0043 protein ytxB UniRef100_P06568Bacillus subtilis YtxB [Bacillus subtilis] 2933 Primosomal protein dnaI[Bacillus UniRef100_P06567 Bacillus subtilis DnaI subtilis] 2934Replication initiation and membrane UniRef100_P07908 Bacillus subtilisDnaB attachment protein [Bacillus subtilis] 2935 Hypothetical UPF0168protein ytcG UniRef100_Q45549 Bacillus subtilis YtcG [Bacillus subtilis]2936 2937 SpeD 2938 GapB 2939 Pectin lyase [Bacillus subtilis]UniRef100_P94449 Bacillus subtilis PelB 2940 Dephospho-CoA kinase[Bacillus UniRef100_O34932 Bacillus subtilis YtaG subtilis] 2941 YtaF2942 Formamidopyrimidine-DNA glycosylase UniRef100_O34403 Bacillussubtilis MutM [Bacillus subtilis] 2943 DNA polymerase I [Bacillussubtilis] UniRef100_O34996 Bacillus subtilis PolA 2944 Alkalinephosphatase synthesis sensor UniRef100_P23545 Bacillus subtilis PhoRprotein phoR [Bacillus subtilis] 2945 Alkaline phosphatase synthesisUniRef100_P13792 Bacillus subtilis PhoP transcriptional regulatoryprotein phoP [Bacillus subtilis] 2946 Malate dehydrogenase [BacillusUniRef100_P49814 Bacillus subtilis Mdh subtilis] 2947 Isocitratedehydrogenase [NADP] UniRef100_P39126 NADP Icd [Bacillus subtilis] 2948Citrate synthase II [Bacillus subtilis] UniRef100_P39120 Bacillussubtilis CitZ 2949 YtwI [Bacillus subtilis] UniRef100_O34811 Bacillussubtilis YtwI 2950 Hypothetical UPF0118 protein ytvI UniRef100_O34991Bacillus subtilis YtvI [Bacillus subtilis] 2951 YtzA protein [Bacillussubtilis] UniRef100_O32064 Bacillus subtilis YtzA 2952 Pyk 29536-phosphofructokinase [Bacillus UniRef100_O34529 Bacillus subtilis PfkAsubtilis] 2954 Acetyl-coenzyme A carboxylase UniRef100_O34847 Bacillussubtilis AccA carboxyl transferase subunit alpha [Bacillus subtilis]2955 Acetyl-CoA carboxylase subunit UniRef100_O34571 Bacillus subtilisAccD [Bacillus subtilis] 2956 YtsJ 2957 DNA polymerase III alpha subunitUniRef100_O34623 Bacillus subtilis DnaE [Bacillus subtilis] 2958Hypothetical Membrane Spanning UniRef100_Q812P3 Bacillus cereus Protein[Bacillus cereus] 2959 YtrI [Bacillus subtilis] UniRef100_O34460Bacillus subtilis YtrI 2960 BH3172 protein [Bacillus halodurans]UniRef100_Q9K835 Bacillus halodurans 2961 YtqI [Bacillus subtilis]UniRef100_O34600 Bacillus subtilis YtqI 2962 YtpI [Bacillus subtilis]UniRef100_O34922 Bacillus subtilis 2963 YtoI 2964 PadR 2965 YtkL protein[Bacillus subtilis] UniRef100_Q795U4 Bacillus subtilis YtkL 2966 YtkK2967 2968 Argininosuccinate lyase [Bacillus UniRef100_O34858 Bacillussubtilis ArgH subtilis] 2969 Argininosuccinate synthase [BacillusUniRef100_O34347 Bacillus subtilis ArgG subtilis] 2970 Molybdenumcofactor biosynthesis UniRef100_O34457 Bacillus subtilis MoaB protein B[Bacillus subtilis] 2971 AckA 2972 Hypothetical protein ytxK [BacillusUniRef100_P37876 Bacillus subtilis YtxK subtilis] 2973 Probable thiolperoxidase [Bacillus UniRef100_P80864 Bacillus subtilis Tpx subtilis]2974 YtfJ [Bacillus subtilis] UniRef100_O34806 Bacillus subtilis YtfJ2975 YtfI 2976 YteJ [Bacillus subtilis] UniRef100_O34424 Bacillussubtilis YteJ 2977 Putative signal peptide peptidase sppAUniRef100_O34525 Bacillus subtilis SppA [Bacillus subtilis] 2978Probable inorganic polyphosphate/ATP- UniRef100_O34934 Bacillus subtilisYtdI NAD kinase 2 (EC 2.7.1.23) (Poly(P)/ATP NAD kinase 2) [Bacillussubtilis] 2979 YhbJ protein [Bacillus subtilis] UniRef100_O31593Bacillus subtilis YhbJ 2980 Multidrug resistance proteinUniRef100_Q8CQB1 Staphylococcus YubD [Staphylococcus epidermidis]epidermidis 2981 Putative HTH-type transcriptional UniRef100_P42103Bacillus subtilis YxaD regulator yxaD [Bacillus subtilis] 2982 YtcI[Bacillus subtilis] UniRef100_O34613 Bacillus subtilis YtcI 2983 Small,acid-soluble spore protein 1 UniRef100_P06552 Bacillusstearothermophilus [Bacillus stearothermophilus] 2984 Probable thiaminebiosynthesis protein UniRef100_O34595 Bacillus subtilis YtbJ thil[Bacillus subtilis] 2985 NifS2 [Bacillus subtilis] UniRef100_O34874Bacillus subtilis NifZ 2986 Branched-chain amino acid transportUniRef100_O34545 Bacillus subtilis BraB system carrier protein braB[Bacillus subtilis] 2987 IS1627s1-related, transposase [BacillusUniRef100_Q7CMD0 Bacillus anthracis str. A2012 anthracis str. A2012]2988 UPI00003CC069 UniRef100 entry UniRef100_UPI00003CC069 2989Septation ring formation regulator ezrA UniRef100_O34894 Bacillussubtilis EzrA [Bacillus subtilis] 2990 Histidinol-phosphatase [BacillusUniRef100_O34411 Bacillus subtilis HisJ subtilis] 2991 Probable HTH-typetranscriptional UniRef100_O34970 Bacillus subtilis YttP regulator yttP[Bacillus subtilis] 2992 Hypothetical conserved protein UniRef100_Q8EPB0Oceanobacillus iheyensis [Oceanobacillus iheyensis] 2993 YtrP [Bacillussubtilis] UniRef100_O34325 Bacillus subtilis YtrP 2994 30S ribosomalprotein S4 [Bacillus UniRef100_P21466 Bacillus subtilis RpsD subtilis]2995 2996 2997 YddR 2998 HTH-type transcriptional regulator lrpAUniRef100_P96652 Bacillus subtilis LrpA [Bacillus subtilis] 2999 3000Tyrosyl-tRNA synthetase 1 [Bacillus UniRef100_P22326 Bacillus subtilisTyrS subtilis] 3001 Acetyl-coenzyme A synthetase [BacillusUniRef100_P39062 Bacillus subtilis AcsA subtilis] 3002 Acetoinutilization protein acuA [Bacillus UniRef100_P39065 Bacillus subtilisAcuA subtilis] 3003 Acetoin utilization acuB protein [BacillusUniRef100_P39066 Bacillus subtilis AcuB subtilis] 3004 Acetoinutilization protein acuC UniRef100_P39067 Bacillus subtilis AcuC[Bacillus subtilis] 3005 Hypothetical protein ytxE [BacillusUniRef100_P39064 Bacillus subtilis YtxE subtilis] 3006 Hypotheticalprotein ytxD [Bacillus UniRef100_P39063 Bacillus subtilis YtxD subtilis]3007 Catabolite control protein A [Bacillus UniRef100_P25144 Bacillussubtilis CcpA subtilis] 3008 AroA(G) protein [Includes: Phospho-2-UniRef100_P39912 Includes: AroA dehydro-3-deoxyheptonate aldolasePhospho-2- (EC 2.5.1.54) (Phospho-2-keto-3- dehydro-3- deoxyheptonatealdolase) (DAHP deoxyheptonate synthetase) (3-deoxy-D-arabino- aldolase(EC heptulosonate 7-phosphate synthase); 2.5.1.54) Chorismate mutase (EC5.4.99.5)] (Phospho-2-keto- [Bacillus subtilis] 3- deoxyheptonatealdolase) (DAHP synthetase) (3- deoxy-D-arabino- heptulosonate 7-phosphate synthase); Chorismate mutase (EC 5.4.99.5) 3009 Similar tohypothetical repeat UniRef100_Q7N3B8 Photorhabdus luminescens containingprotein [Photorhabdus luminescens] 3010 Hypothetical protein ytxJ[Bacillus UniRef100_P39914 Bacillus subtilis YtxJ subtilis] 3011Hypothetical protein ytxH [Bacillus UniRef100_P40780 Bacillus subtilisYtxH subtilis] 3012 Hypothetical protein ytxG [Bacillus UniRef100_P40779Bacillus subtilis YtxG subtilis] 3013 MurC 3014 YtpT [Bacillus subtilis]UniRef100_O34749 Bacillus subtilis YtpT 3015 YtpR [Bacillus subtilis]UniRef100_O34943 Bacillus subtilis YtpR 3016 YtpQ [Bacillus subtilis]UniRef100_O34496 Bacillus subtilis YtpQ 3017 Putative thioredoxin[Bacillus subtilis] UniRef100_O34357 Bacillus subtilis YtpP 3018 YtoQ[Bacillus subtilis] UniRef100_O34305 Bacillus subtilis YtoQ 3019 YtoP[Bacillus subtilis] UniRef100_O34924 Bacillus subtilis YtoP 3020 YtzBprotein [Bacillus subtilis] UniRef100_O32065 Bacillus subtilis YtzB 3021Probable NAD-dependent malic UniRef100_O34389 Bacillus subtilis MalSenzyme 3 [Bacillus subtilis] 3022 YtnP [Bacillus subtilis]UniRef100_O34760 Bacillus subtilis YtnP 3023 tRNA (guanine-N(7)-)-UniRef100_O34522 Bacillus subtilis YtmQ methyltransferase (EC 2.1.1.33)(tRNA(m7G46)-methyltransferase) [Bacillus subtilis] 3024 YtzH protein[Bacillus subtilis] UniRef100_O32066 Bacillus subtilis 3025 YtmP[Bacillus subtilis] UniRef100_O34935 Bacillus subtilis YtmP 3026 AmyXprotein [Bacillus subtilis] UniRef100_O34587 Bacillus subtilis AmyX 3027YtlR [Bacillus subtilis] UniRef100_O34799 Bacillus subtilis YtlR 3028YtlQ [Bacillus subtilis] UniRef100_O34471 Bacillus subtilis YtlQ 3029Hypothetical UPF0097 protein ytlP UniRef100_O34570 Bacillus subtilisYtlP [Bacillus subtilis] 3030 Probable cysteine synthase (ECUniRef100_O34476 Bacillus subtilis YtkP 2.5.1.47) (O-acetylserinesulfhydrylase) (O-acetylserine (Thiol)-lyase) [Bacillus subtilis] 3031Hypothetical protein [Bacillus cereus] UniRef100_Q81BR8 Bacillus cereusYncE 3032 Putative peptidase [Bacillus subtilis] UniRef100_O34944Bacillus subtilis YtjP 3033 YtiP [Bacillus subtilis] UniRef100_O34978Bacillus subtilis YtiP 3034 YtzE protein [Bacillus subtilis]UniRef100_O32067 Bacillus subtilis 3035 Ribosomal small subunitpseudouridine UniRef100_Q816W1 Bacillus cereus YtzF synthase A [Bacilluscereus] 3036 YtgP [Bacillus subtilis] UniRef100_O34674 Bacillus subtilisYtgP 3037 YtfP [Bacillus subtilis] UniRef100_O30505 Bacillus subtilisYtfP 3038 OpuD 3039 Protein cse60 [Bacillus subtilis] UniRef100_P94496Bacillus subtilis 3040 Rhodanese-like domain protein UniRef100_Q72YZ9Bacillus cereus [Bacillus cereus] 3041 RapA 3042 Hypothetical protein[Bacillus UniRef100_Q6HI31 Bacillus thuringiensis thuringiensis] 3043YteU [Bacillus subtilis] UniRef100_O34378 Bacillus subtilis YteU 3044YteT 3045 YteS 3046 YteR [Bacillus subtilis] UniRef100_O34559 Bacillussubtilis YteR 3047 Transmembrane lipoprotein [Bacillus UniRef100_Q9KFJ5Bacillus LplB halodurans] halodurans 3048 YtdP protein [Bacillussubtilis] UniRef100_O32071 Bacillus subtilis YtdP 3049 YtcQ protein[Bacillus subtilis] UniRef100_Q795R2 Bacillus subtilis YtcQ 3050 YtcP3051 Hypothetical protein ytbQ [Bacillus UniRef100_P53560 Bacillussubtilis YtbQ subtilis] 3052 YtaP [Bacillus subtilis] UniRef100_O34973Bacillus subtilis YtaP 3053 Amino acid/polyamine transporter;UniRef100_Q6LYX9 Methanococcus YecA family I [Methanococcus maripaludis]maripaludis 3054 Transcriptional regulator, LysR family UniRef100_Q97DX1Clostridium YwqM [Clostridium acetobutylicum] acetobutylicum 3055 Prolylendopeptidase [Bacillus cereus] UniRef100_Q81C54 Bacillus cereus YycE3056 Leucyl-tRNA synthetase [Bacillus UniRef100_P36430 Bacillus subtilisLeuS subtilis] 3057 YtvB 3058 YttB [Bacillus subtilis] UniRef100_O34546Bacillus subtilis YttB 3059 Lipoprotein [Oceanobacillus iheyensis]UniRef100_Q8EPK3 Oceanobacillus YusA iheyensis 3060 YttA [Bacillussubtilis] UniRef100_O30500 Bacillus subtilis YttA 3061 YtrF [Bacillussubtilis] UniRef100_O35005 Bacillus subtilis YtrF 3062 Hypothetical ABCtransporter ATP- UniRef100_O34392 Bacillus subtilis YtrE binding proteinytrE [Bacillus subtilis] 3063 YtrC [Bacillus subtilis] UniRef100_O34898Bacillus subtilis YtrC 3064 Transporter [Bacillus subtilis]UniRef100_O34641 Bacillus subtilis YtrB 3065 Transcription regulator[Bacillus subtilis] UniRef100_O34712 Bacillus subtilis YtrA 3066Hypothetical protein ytzC [Bacillus UniRef100_O32073 Bacillus subtilissubtilis] 3067 YtqA [Bacillus subtilis] UniRef100_O35008 Bacillussubtilis YtqA 3068 YtqB 3069 Proton glutamate symport proteinUniRef100_P39817 Bacillus subtilis GltP [Bacillus subtilis] 3070Hypothetical protein ytpB [Bacillus UniRef100_O34707 Bacillus subtilisYtpB subtilis] 3071 Probable lysophospholipase [BacillusUniRef100_O34705 Bacillus subtilis YtpA subtilis] 3072 YtoA [Bacillussubtilis] UniRef100_O34696 Bacillus subtilis YtoA 3073 YwoA 3074Glycosyl transferase, group 1 family UniRef100_Q6HCB9 Bacillus TuaC[Bacillus thuringiensis] thuringiensis 3075 Asparagine synthetase[glutamine- UniRef100_P54420 glutamine- AsnB hydrolyzing] 1 [Bacillussubtilis] hydrolyzing 3076 S-adenosylmethionine synthetaseUniRef100_P54419 Bacillus subtilis MetK [Bacillus subtilis] 3077Phosphoenolpyruvate carboxykinase UniRef100_P54418 ATP PckA [ATP][Bacillus subtilis] 3078 Sodium:dicarboxylate symporter UniRef100_Q8EP16Oceanobacillus DctP [Oceanobacillus iheyensis] iheyensis 3079Hypothetical protein ytmB [Bacillus UniRef100_O34365 Bacillus subtilissubtilis] 3080 Putative peptidase [Bacillus subtilis] UniRef100_O34493Bacillus subtilis YtmA 3081 ABC transporter substrate-bindingUniRef100_Q816P5 Bacillus cereus YtlA protein [Bacillus cereus] 3082Putative transporter [Bacillus subtilis] UniRef100_O34314 Bacillussubtilis YtlC 3083 YtlD 3084 YtkD [Bacillus subtilis] UniRef100_O35013Bacillus subtilis YtkD 3085 Hypothetical protein [BacillusUniRef100_Q6HC91 Bacillus thuringiensis thuringiensis] 3086 Hypotheticalprotein ytkC [Bacillus UniRef100_O34883 Bacillus subtilis YtkC subtilis]3087 General stress protein 20U [Bacillus UniRef100_P80879 Bacillussubtilis Dps subtilis] 3088 Hypothetical protein ytkA [BacillusUniRef100_P40768 Bacillus subtilis YtkA subtilis] 3089S-ribosylhomocysteinase [Bacillus UniRef100_O34667 Bacillus subtilisLuxS subtilis] 3090 Hypothetical UPF0161 protein ytjA UniRef100_O34601Bacillus subtilis [Bacillus subtilis] 3091 YtiB [Bacillus subtilis]UniRef100_O34872 Bacillus subtilis YtiB 3092 Low-affinity zinc transportprotein UniRef100_Q81F90 Bacillus cereus YciC [Bacillus cereus] 3093High-affinity zinc uptake system protein UniRef100_Q81EF8 Bacilluscereus YcdH znuA [Bacillus cereus] 3094 50S ribosomal protein L31 type BUniRef100_O34967 Bacillus subtilis [Bacillus subtilis] 3095 YthA[Bacillus subtilis] UniRef100_O34655 Bacillus subtilis YthA 3096 YthB[Bacillus subtilis] UniRef100_O34505 Bacillus subtilis YthB 3097Hypothetical protein [Bacillus cereus] UniRef100_Q737J1 Bacillus cereus3098 3099 O-succinylbenzoate synthase (EC UniRef100_O34514 Bacillussubtilis MenC 4.2.1.—) (OSB synthase) (OSBS) (4-(2′-carboxyphenyl)-4-oxybutyric acid synthase) [Bacillus subtilis] 3100O-succinylbenzoate--CoA ligase UniRef100_P23971 Bacillus subtilis MenE[Bacillus subtilis] 3101 Naphthoate synthase [Bacillus subtilis]UniRef100_P23966 Bacillus subtilis MenB 3102 YtxM 3103 Menaquinonebiosynthesis protein UniRef100_P23970 Includes: 2- MenD menD [Includes:2-succinyl-6-hydroxy- succinyl-6- 2,4-cyclohexadiene-1-carboxylatehydroxy-2,4- synthase (EC 2.5.1.64) (SHCHC cyclohexadiene- synthase);2-oxoglutarate 1-carboxylate decarboxylase (EC 4.1.1.71) (Alpha-synthase (EC ketoglutarate decarboxylase) (KDC)] 2.5.1.64) [Bacillussubtilis] (SHCHC synthase); 2- oxoglutarate decarboxylase (EC 4.1.1.71)(Alpha- ketoglutarate decarboxylase) (KDC) 3104 Menaquinone-specificisochorismate UniRef100_P23973 Bacillus subtilis MenF synthase [Bacillussubtilis] 3105 Probable 1,4-dihydroxy-2-naphthoate UniRef100_P39582Bacillus subtilis MenA octaprenyltransferase [Bacillus subtilis] 3106Hypothetical protein yteA [Bacillus UniRef100_P42408 Bacillus subtilisYteA subtilis] 3107 Glycogen phosphorylase [Bacillus UniRef100_P39123Bacillus subtilis GlgP subtilis] 3108 Glycogen synthase [Bacillussubtilis] UniRef100_P39125 Bacillus subtilis GlgA 3109 Glycogenbiosynthesis protein glgD UniRef100_P39124 Bacillus subtilis GlgD[Bacillus subtilis] 3110 Glucose-1-phosphate UniRef100_P39122 Bacillussubtilis GlgC adenylyltransferase [Bacillus subtilis] 31111,4-alpha-glucan branching enzyme UniRef100_P39118 Bacillus subtilisGlgB [Bacillus subtilis] 3112 AraR 3113 YuaJ protein [Bacillus subtilis]UniRef100_O32074 Bacillus subtilis YuaJ 3114 BH4010 protein [Bacillushalodurans] UniRef100_Q9K5S8 Bacillus YhcS halodurans 3115 BH4011protein [Bacillus halodurans] UniRef100_Q9K5S7 Bacillus halodurans 31163117 RapD 3118 Pyrrolidone-carboxylate peptidase UniRef100_P46107Bacillus Pcp [Bacillus amyloliquefaciens] amyloliquefaciens 3119 BH0597protein [Bacillus halodurans] UniRef100_Q9KF88 Bacillus YuaA halodurans3120 YubG 3121 YxxF 3122 YuaE protein [Bacillus subtilis]UniRef100_O32078 Bacillus subtilis YuaE 3123 YuaD protein [Bacillussubtilis] UniRef100_O32079 Bacillus subtilis YuaD 3124 Alcoholdehydrogenase [Bacillus UniRef100_P71017 Bacillus subtilis GbsBsubtilis] 3125 Betaine aldehyde dehydrogenase UniRef100_P71016 Bacillussubtilis GbsA [Bacillus subtilis] 3126 Hypothetical protein yuaC[Bacillus UniRef100_P71015 Bacillus subtilis YuaC subtilis] 3127UPI00002D3D35 UniRef100 entry UniRef100_UPI00002D3D35 OpuE 3128Hypothetical protein yktD [Bacillus UniRef100_Q45500 Bacillus subtilisYktD subtilis] 3129 Alanine racemase 2 [Bacillus subtilis]UniRef100_P94494 Bacillus subtilis YncD 3130 Oxalate decarboxylase[Bacillus UniRef100_Q81GZ6 Bacillus cereus YoaN cereus] 3131Hypothetical protein CAC0135 UniRef100_Q97MQ7 Clostridium acetobutylicum[Clostridium acetobutylicum] 3132 Hypothetical protein [BacillusUniRef100_Q6HGC9 Bacillus thuringiensis thuringiensis] 3133 Hypotheticalprotein [Bacillus UniRef100_Q6HGC8 Bacillus thuringiensis thuringiensis]3134 3135 Hypothetical protein [Bacillus UniRef100_Q6HGC6 Bacillusthuringiensis thuringiensis] 3136 Hypothetical protein [BacillusUniRef100_Q6HGC5 Bacillus thuringiensis thuringiensis] 3137 Hypotheticalconserved protein UniRef100_Q8ETF5 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 3138 3139 3140 YkoN [Bacillus subtilis]UniRef100_O34625 Bacillus subtilis YkoN 3141 Hypothetical protein ykoP[Bacillus UniRef100_O34495 Bacillus subtilis YkoP subtilis] 3142Hypothetical UPF0151 protein ykoQ UniRef100_O35040 Bacillus subtilisYkoQ [Bacillus subtilis] 3143 Undecaprenyl-diphosphatase [BacillusUniRef100_P94507 Bacillus subtilis YubB subtilis] 3144 HypotheticalUPF0118 protein yubA UniRef100_O32086 Bacillus subtilis YubA [Bacillussubtilis] 3145 Hypothetical oxidoreductase yulF UniRef100_O05265Bacillus subtilis YulF [Bacillus subtilis] 3146 Lmo2256 protein[Listeria UniRef100_Q929B9 Listeria YraA monocytogenes] monocytogenes3147 McpA 3148 McpA 3149 McpA 3150 Protein-glutamine gamma-UniRef100_P40746 Bacillus subtilis Tgl glutamyltransferase [Bacillussubtilis] 3151 2-nitropropane dioxygenase [Bacillus UniRef100_O05413Bacillus subtilis YrpB subtilis] 3152 Hypothetical UPF0047 protein yugUUniRef100_O05243 Bacillus subtilis YugU [Bacillus subtilis] 3153Hypothetical protein yugT [Bacillus UniRef100_O05242 Bacillus subtilisYugT subtilis] 3154 Transcriptional regulator, TetR familyUniRef100_Q81GX6 Bacillus cereus YfiR [Bacillus cereus] 3155Hypothetical protein yqeB [Bacillus UniRef100_P54447 Bacillus subtilisYqeB subtilis] 3156 Beta(1,4)-glucan glucanohydrolase UniRef100_Q6D3B7Erwinia carotovora [Erwinia carotovora] 3157 Hypothetical UPF0053protein yugS UniRef100_O05241 Bacillus subtilis YugS [Bacillus subtilis]3158 Hypothetical protein yugP [Bacillus UniRef100_O05248 Bacillussubtilis YugP subtilis] 3159 YugO protein [Bacillus subtilis]UniRef100_Q795M8 Bacillus subtilis 3160 Hypothetical protein yugN[Bacillus UniRef100_O05246 Bacillus subtilis YugN subtilis] 3161Hypothetical protein [Bacillus UniRef100_Q6HIW1 Bacillus YdfRthuringiensis] thuringiensis 3162 YtaB protein [Bacillus subtilis]UniRef100_O34694 Bacillus subtilis YtaB 3163 Predicted acetyltransferase[Clostridium UniRef100_Q97IT3 Clostridium YkkB acetobutylicum]acetobutylicum 3164 Glucose-6-phosphate isomerase UniRef100_P80860Bacillus subtilis Pgi [Bacillus subtilis] 3165 Probable NADH-dependentbutanol UniRef100_O05239 Bacillus subtilis YugJ dehydrogenase 1[Bacillus subtilis] 3166 YuzA protein [Bacillus subtilis]UniRef100_O32087 Bacillus subtilis 3167 General stress protein 13[Bacillus UniRef100_P80870 Bacillus subtilis YugI subtilis] 3168 Alaninetransaminase [Bacillus subtilis] UniRef100_Q795M6 Bacillus subtilis AlaT3169 Transcriptional regulator [Bacillus UniRef100_O05236 Bacillussubtilis AlaR subtilis] 3170 Hypothetical protein yugF [BacillusUniRef100_O05235 Bacillus subtilis YugF subtilis] 3171 Hypotheticalprotein yugE [Bacillus UniRef100_O05234 Bacillus subtilis subtilis] 3172Hypothetical protein SMU.305 UniRef100_Q9X669 Streptococcus mutans[Streptococcus mutans] 3173 Putative aminotransferase B [BacillusUniRef100_Q08432 Bacillus subtilis PatB subtilis] 3174 3175Kinase-associated lipoprotein B UniRef100_Q08429 Bacillus subtilis KapBprecursor [Bacillus subtilis] 3176 Hypothetical protein yugB [BacillusUniRef100_O05231 Bacillus subtilis KapD subtilis] 3177 YuxJ 3178 PbpD3179 Hypothetical protein yuxK [Bacillus UniRef100_P40761 Bacillussubtilis YuxK subtilis] 3180 Hypothetical protein yufK [BacillusUniRef100_O05249 Bacillus subtilis YufK subtilis] 3181 Hypotheticalprotein yufL [Bacillus UniRef100_O05250 Bacillus subtilis YufL subtilis]3182 Hypothetical protein yufM [Bacillus UniRef100_O05251 Bacillussubtilis YufM subtilis] 3183 3184 UPI00003CB938 UniRef100 entryUniRef100_UPI00003CB938 PssA 3185 Hypothetical protein ybfM [BacillusUniRef100_O31453 Bacillus subtilis YbfM subtilis] 3186Phosphatidylserine decarboxylase UniRef100_Q6HDI5 Bacillus Psd [Bacillusthuringiensis] thuringiensis 3187 UPI00003CC069 UniRef100 entryUniRef100_UPI00003CC069 3188 IS1627s1-related, transposase [BacillusUniRef100_Q7CMD0 Bacillus anthracis str. A2012 anthracis str. A2012]3189 Na(+)-malate symporter [Bacillus UniRef100_O05256 Bacillus subtilisMaeN subtilis] 3190 3191 Na(+)/H(+) antiporter subunit AUniRef100_Q9K2S2 Bacillus subtilis MrpA [Bacillus subtilis] 3192Na(+)/H(+) antiporter subunit B UniRef100_O05259 Bacillus subtilis MrpB[Bacillus subtilis] 3193 Na(+)/H(+) antiporter subunit CUniRef100_O05260 Bacillus subtilis MrpC [Bacillus subtilis] 3194Na(+)/H(+) antiporter subunit D UniRef100_O05229 Bacillus subtilis MrpD[Bacillus subtilis] 3195 Na(+)/H(+) antiporter subunit EUniRef100_Q7WY60 Bacillus subtilis MrpE [Bacillus subtilis] 3196Na(+)/H(+) antiporter subunit F UniRef100_O05228 Bacillus subtilis[Bacillus subtilis] 3197 Na(+)/H(+) antiporter subunit GUniRef100_O05227 Bacillus subtilis MrpG [Bacillus subtilis] 3198 YuxO3199 ComA 3200 ComP 3201 IS1627s1-related, transposase [BacillusUniRef100_Q7CMD0 Bacillus anthracis str. A2012 anthracis str. A2012]3202 UPI00003CC069 UniRef100 entry UniRef100_UPI00003CC069 3203 ComP3204 3205 ComQ [Bacillus subtilis] UniRef100_Q9K5L3 Bacillus subtilisComQ 3206 3207 Hypothetical protein yuzC [Bacillus UniRef100_O32089Bacillus subtilis YuzC subtilis] 3208 Hypothetical protein yuxH[Bacillus UniRef100_P14203 Bacillus subtilis YuxH subtilis] 3209 YueKprotein [Bacillus subtilis] UniRef100_O32090 Bacillus subtilis YueK 3210YueJ protein [Bacillus subtilis] UniRef100_O32091 Bacillus subtilis YueJ3211 YueI 3212 3213 Hypothetical protein yueG [Bacillus UniRef100_O32094Bacillus subtilis subtilis] 3214 YueF 3215 RRF2 family protein [Bacilluscereus] UniRef100_Q81EX1 Bacillus cereus YwnA 3216 Probablelipase/esterase UniRef100_Q7UUA2 Rhodopirellula YuxL [Rhodopirellulabaltica] baltica 3217 BH1896 protein [Bacillus halodurans]UniRef100_Q9KBN0 Bacillus halodurans 3218 YueE protein [Bacillussubtilis] UniRef100_O32098 Bacillus subtilis YueE 3219 YueD protein[Bacillus subtilis] UniRef100_O32099 Bacillus subtilis YueD 3220Hypothetical protein yueC [Bacillus UniRef100_O32100 Bacillus subtilisYueC subtilis] 3221 YueB protein [Bacillus subtilis] UniRef100_O32101Bacillus subtilis YueB 3222 YukA protein [Bacillus subtilis]UniRef100_P71068 Bacillus subtilis YukA 3223 YukC protein [Bacillussubtilis] UniRef100_P71070 Bacillus subtilis YukC 3224 YukD protein[Bacillus subtilis] UniRef100_P71071 Bacillus subtilis 3225 Lin0049protein [Listeria innocua] UniRef100_Q92FQ4 Listeria innocua 3226 YflA3227 YukF protein [Bacillus subtilis] UniRef100_P71073 Bacillus subtilisYukF 3228 Alanine dehydrogenase [Bacillus UniRef100_Q08352 Bacillussubtilis Ald subtilis] 3229 3230 YuiH protein [Bacillus subtilis]UniRef100_O32103 Bacillus subtilis YuiH 3231 YuiG protein [Bacillussubtilis] UniRef100_O32104 Bacillus subtilis YuiG 3232 YuiF protein[Bacillus subtilis] UniRef100_O32105 Bacillus subtilis YuiF 3233Probable cytosol aminopeptidase UniRef100_O32106 Bacillus subtilis YuiE[Bacillus subtilis] 3234 YuiD protein [Bacillus subtilis]UniRef100_O32107 Bacillus subtilis YuiD 3235 YuiC protein [Bacillussubtilis] UniRef100_O32108 Bacillus subtilis YuiC 3236 YuiB protein[Bacillus subtilis] UniRef100_O32109 Bacillus subtilis YuiB 3237 3238YumB 3239 Thioredoxine reductase [Bacillus UniRef100_O05268 Bacillussubtilis YumC subtilis] 3240 3241 YdjO protein [Bacillus subtilis]UniRef100_O34759 Bacillus subtilis 3242 YxbD 3243 Hypothetical proteinyutM [Bacillus UniRef100_O32113 Bacillus subtilis YutM subtilis] 3244Diaminopimelate epimerase [Bacillus UniRef100_O32114 Bacillus subtilisDapF subtilis] 3245 YutK protein [Bacillus subtilis] UniRef100_O32115Bacillus subtilis YutK 3246 YuzB protein [Bacillus subtilis]UniRef100_O32116 Bacillus subtilis 3247 YutJ protein [Bacillus subtilis]UniRef100_O32117 Bacillus subtilis YutJ 3248 YdhG protein [Bacillussubtilis] UniRef100_O05499 Bacillus subtilis YdhG 3249 Responseregulator aspartate UniRef100_Q9KBE1 Bacillus RapI phosphatase [Bacillushalodurans] halodurans 3250 Phenolic acid decarboxylase [BacillusUniRef100_O07006 Bacillus subtilis PadC subtilis] 3251 BH2266 protein[Bacillus halodurans] UniRef100_Q9KAM1 Bacillus halodurans 3252 YuzDprotein [Bacillus subtilis] UniRef100_O32118 Bacillus subtilis YuzD 3253YutI protein [Bacillus subtilis] UniRef100_O32119 Bacillus subtilis 3254Probable peptidase yuxL [Bacillus UniRef100_P39839 Bacillus subtilisYuxL subtilis] 3255 Homoserine kinase [Bacillus subtilis]UniRef100_P04948 Bacillus subtilis ThrB 3256 Threonine synthase[Bacillus subtilis] UniRef100_P04990 Bacillus subtilis ThrC 3257Homoserine dehydrogenase [Bacillus UniRef100_P19582 Bacillus subtilisHom subtilis] 3258 Glycerate dehydrogenase UniRef100_Q8ENW9Oceanobacillus YvcT [Oceanobacillus iheyensis] iheyensis 3259 YutHprotein [Bacillus subtilis] UniRef100_O32123 Bacillus subtilis YutH 3260Hypothetical protein yutG [Bacillus UniRef100_O32124 Bacillus subtilisYutG subtilis] 3261 YutF protein [Bacillus subtilis] UniRef100_O32125Bacillus subtilis YutF 3262 YutE protein [Bacillus subtilis]UniRef100_O32126 Bacillus subtilis YutE 3263 YutD protein [Bacillussubtilis] UniRef100_O32127 Bacillus subtilis 3264 YutC protein [Bacillussubtilis] UniRef100_O32128 Bacillus subtilis YutC 3265 LipA 3266 YunAprotein [Bacillus subtilis] UniRef100_O32130 Bacillus subtilis YunA 32673268 Sodium-dependent transporter [Bacillus UniRef100_Q9K7C5 BacillusYocR halodurans] halodurans 3269 YunB protein [Bacillus subtilis]UniRef100_O32131 Bacillus subtilis YunB 3270 YunC protein [Bacillussubtilis] UniRef100_O32132 Bacillus subtilis YunC 3271 YunD protein[Bacillus subtilis] UniRef100_O32133 Bacillus subtilis YunD 3272 YunEprotein [Bacillus subtilis] UniRef100_O32134 Bacillus subtilis YunE 3273YunF protein [Bacillus subtilis] UniRef100_O32135 Bacillus subtilis YunF3274 YmcC 3275 TetR family transcriptional regulator? UniRef100_Q67KA4Symbiobacterium PksA [Symbiobacterium thermophilum] thermophilum 32763277 Purine catabolism protein pucG UniRef100_O32148 Bacillus subtilisYurG [Bacillus subtilis] 3278 Allantoate amidohydrolase [BacillusUniRef100_O32149 Bacillus subtilis YurH subtilis] 3279 Purine catabolismregulatory protein UniRef100_O32138 Bacillus subtilis PucR [Bacillussubtilis] 3280 Multidrug resistance protein B [Bacillus UniRef100_Q63FH7Bacillus cereus Blt cereus ZK] ZK 3281 BH2308 protein [Bacillushalodurans] UniRef100_Q9KAH9 Bacillus YcgA halodurans 3282 TrpD 3283Anthranilate phosphoribosyltransferase UniRef100_Q8U089 Pyrococcusfuriosus [Pyrococcus furiosus] 3284 Extracellular ribonuclease precursorUniRef100_O32150 Bacillus subtilis YurI [Bacillus subtilis] 3285 BH1977protein [Bacillus halodurans] UniRef100_Q9KBF1 Bacillus halodurans 3286YurR 3287 Putative membrane protein [Bordetella UniRef100_Q7WGW7Bordetella bronchiseptica bronchiseptica] 3288 UPI00003CB453 UniRef100entry UniRef100_UPI00003CB453 3289 Response regulator aspartateUniRef100_P96649 Bacillus subtilis RapI phosphatase I [Bacillussubtilis] 3290 YurU protein [Bacillus subtilis] UniRef100_O32162Bacillus subtilis YurU 3291 NifU-like protein [Bacillus subtilis]UniRef100_O32163 Bacillus subtilis YurV 3292 Probable cysteinedesulfurase [Bacillus UniRef100_O32164 Bacillus subtilis Csd subtilis]3293 YurX protein [Bacillus subtilis] UniRef100_O32165 Bacillus subtilisYurX 3294 Vegetative protein 296 [Bacillus subtilis] UniRef100_P80866Bacillus subtilis YurY 3295 Lmo2575 protein [Listeria UniRef100_Q8Y480Listeria CzcD monocytogenes] monocytogenes 3296 3297 BH3473 protein[Bacillus halodurans] UniRef100_Q9K796 Bacillus YurZ halodurans 3298YusA protein [Bacillus subtilis] UniRef100_O32167 Bacillus subtilis YusA3299 YusB protein [Bacillus subtilis] UniRef100_O32168 Bacillus subtilisYusB 3300 YusC protein [Bacillus subtilis] UniRef100_O32169 Bacillussubtilis YusC 3301 Hypothetical protein yusD [Bacillus UniRef100_O32170Bacillus subtilis YusD subtilis] 3302 YusE protein [Bacillus subtilis]UniRef100_O32171 Bacillus subtilis 3303 YusF protein [Bacillus subtilis]UniRef100_O32172 Bacillus subtilis YusF 3304 3305 Glycine cleavagesystem H protein UniRef100_O32174 Bacillus subtilis GcvH [Bacillussubtilis] 3306 Hypothetical protein yusI [Bacillus UniRef100_O32175Bacillus subtilis YusI subtilis] 3307 YusJ protein [Bacillus subtilis]UniRef100_O32176 Bacillus subtilis YusJ 3308 YusK protein [Bacillussubtilis] UniRef100_O32177 Bacillus subtilis YusK 3309 YusL protein[Bacillus subtilis] UniRef100_O32178 Bacillus subtilis YusL 3310 33113312 YusN protein [Bacillus subtilis] UniRef100_O32180 Bacillus subtilisYusN 3313 Hypothetical protein yusU [Bacillus UniRef100_O32187 Bacillussubtilis subtilis] 3314 BH1040 protein [Bacillus halodurans]UniRef100_Q9KE18 Bacillus halodurans 3315 YusV protein [Bacillussubtilis] UniRef100_O32188 Bacillus subtilis YusV 3316 YfhA 3317 YfiZprotein [Bacillus subtilis] UniRef100_O31568 Bacillus subtilis YfiZ 3318YfiY protein [Bacillus subtilis] UniRef100_O31567 Bacillus subtilis YfiY3319 Hypothetical protein yusW precursor UniRef100_O32189 Bacillussubtilis YusW [Bacillus subtilis] 3320 YusX protein [Bacillus subtilis]UniRef100_O32190 Bacillus subtilis YusX 3321 D-alanyl-D-alaninecarboxypeptidase UniRef100_Q8ERG0 Oceanobacillus DacB [Oceanobacillusiheyensis] iheyensis 3322 Hypothetical oxidoreductase yusZUniRef100_P37959 Bacillus subtilis YusZ [Bacillus subtilis] 3323Metalloregulation DNA-binding stress UniRef100_P37960 Bacillus subtilisMrgA protein [Bacillus subtilis] 3324 Probable serine protease yvtA[Bacillus UniRef100_Q9R9I1 Bacillus subtilis YvtA subtilis] 3325Transcriptional regulatory protein cssR UniRef100_O32192 Bacillussubtilis CssR [Bacillus subtilis] 3326 Sensor protein cssS [Bacillussubtilis] UniRef100_O32193 Bacillus subtilis CssS 3327 YirB [Bacillussubtilis] UniRef100_O32302 Bacillus subtilis 3328 Putative HTH-typetranscriptional UniRef100_P40950 Bacillus subtilis YuxN regulator yuxN[Bacillus subtilis] 3329 Fumarate hydratase class II [BacillusUniRef100_P07343 Bacillus subtilis CitG subtilis] 3330 3331 Sporegermination protein A1 [Bacillus UniRef100_P07868 Bacillus subtilisGerAA subtilis] 3332 Spore germination protein A2 [BacillusUniRef100_P07869 Bacillus subtilis GerAB subtilis] 3333 Sporegermination protein A3 precursor UniRef100_P07870 Bacillus subtilisGerAC [Bacillus subtilis] 3334 3335 YvqC 3336 YvqE protein [Bacillussubtilis] UniRef100_O32198 Bacillus subtilis YvqE 3337 YvqF protein[Bacillus subtilis] UniRef100_O32199 Bacillus subtilis YvqF 3338 YvqGprotein [Bacillus subtilis] UniRef100_O32200 Bacillus subtilis YvqG 3339Hypothetical protein yvqH [Bacillus UniRef100_O32201 Bacillus subtilisYvqH subtilis] 3340 Hypothetical protein yvqI [Bacillus UniRef100_O32202Bacillus subtilis YvqI subtilis] 3341 Pectate lyase P358 [Bacillus sp.P-358] UniRef100_Q8RR73 Bacillus sp. P-358 3342 YvqK protein [Bacillussubtilis] UniRef100_O34899 Bacillus subtilis YvqK 3343 UPI00002E3648UniRef100 entry UniRef100_UPI00002E3648 FabG 3344 3345 GbsB 3346 DapA3347 Putative metal binding protein, YvrA UniRef100_O34631 Bacillussubtilis YvrA [Bacillus subtilis] 3348 Putative hemin permease, YvrBUniRef100_O34451 Bacillus subtilis YvrB [Bacillus subtilis] 3349Putative metal binding protein, YvrC UniRef100_O34805 Bacillus subtilisYvrC [Bacillus subtilis] 3350 Transcriptional regulator, GntR familyUniRef100_Q81SA7 Bacillus YdhC [Bacillus anthracis] anthracis 3351Putative ketoreductase, YvrD [Bacillus UniRef100_O34782 Bacillussubtilis YvrD subtilis] 3352 UPI00003CC410 UniRef100 entryUniRef100_UPI00003CC410 YflK 3353 Transcriptional regulators, LysRfamily UniRef100_Q81DJ6 Bacillus cereus AlsR [Bacillus cereus] 3354Exo-poly-alpha-D-galacturonosidase, UniRef100_Q9WYR8 Thermotoga maritimaputative [Thermotoga maritima] 3355 Altronate hydrolase [Bacillussubtilis] UniRef100_O34673 Bacillus subtilis UxaA 3356 Altronateoxidoreductase [Bacillus UniRef100_O34354 Bacillus subtilis UxaBsubtilis] 3357 LacI repressor-like protein [Bacillus UniRef100_Q9JMQ1Bacillus subtilis ExuR subtilis] 3358 Hypothetical symporter yjmB[Bacillus UniRef100_O34961 Bacillus subtilis YjmB subtilis] 3359 Uronateisomerase [Bacillus subtilis] UniRef100_O34808 Bacillus subtilis UxaC3360 Putative sensory protein kinase, YvrG UniRef100_O34989 Bacillussubtilis YvrG [Bacillus subtilis] 3361 Putative DNA binding responseUniRef100_P94504 Bacillus subtilis YvrH regulator, YvrH [Bacillussubtilis] 3362 Ferrichrome transport ATP-binding UniRef100_P49938Bacillus subtilis FhuC protein fhuC [Bacillus subtilis] 3363 Ferrichrometransport system UniRef100_P49937 Bacillus subtilis FhuG permeaseprotein fhuG [Bacillus subtilis] 3364 Ferrichrome transport systemUniRef100_P49936 Bacillus subtilis FhuB permease protein fhuB [Bacillussubtilis] 3365 Putative arginine ornithine antiporter, UniRef100_O32204Bacillus subtilis YvsH YvsH [Bacillus subtilis] 3366 Hypotheticalprotein yvsG precursor UniRef100_O32205 Bacillus subtilis YvsG [Bacillussubtilis] 3367 Putative molybdate binding protein, UniRef100_O32206Bacillus subtilis YvgJ YvgJ [Bacillus subtilis] 3368 YcnB 3369 Putativereductase protein, YvgN UniRef100_O32210 Bacillus subtilis YvgN[Bacillus subtilis] 3370 Fructokinase [Listeria monocytogenes]UniRef100_Q722A5 Listeria YdhR monocytogenes 3371 Hypothetical proteinycbU precursor UniRef100_P42253 Bacillus subtilis YcbU [Bacillussubtilis] 3372 Hypothetical protein CPE0889 UniRef100_Q8XM01 Clostridiumperfringens [Clostridium perfringens] 3373 YvgS protein [Bacillussubtilis] UniRef100_O32215 Bacillus subtilis YvgS 3374 HypotheticalUPF0126 protein yvgT UniRef100_O32216 Bacillus subtilis YvgT [Bacillussubtilis] 3375 Glutamate-rich protein grpB [Bacillus UniRef100_Q81CT5Bacillus cereus YqkA cereus] 3376 Acetyltransferase, GNAT familyUniRef100_Q6HJN8 Bacillus YuaI [Bacillus thuringiensis] thuringiensis3377 Disulfide bond formation protein C UniRef100_O32217 Bacillussubtilis BdbC [Bacillus subtilis] 3378 Disulfide bond formation proteinD UniRef100_O32218 Bacillus subtilis BdbD precursor [Bacillus subtilis]3379 YvgW protein [Bacillus subtilis] UniRef100_O32219 Bacillus subtilisYvgW 3380 ABC transporter, substrate binding UniRef100_Q8U7T8Agrobacterium YvfK protein [Agrobacterium tumefaciens] tumefaciens 3381Putative sugar transport integral UniRef100_Q9K442 Streptomyces YurNmembrane protein [Streptomyces coelicolor coelicolor] 3382 Putativesugar transport integral UniRef100_Q9K441 Streptomyces YurM membraneprotein [Streptomyces coelicolor coelicolor] 3383 Alpha-glucosidase[Clostridium UniRef100_Q97K36 Clostridium acetobutylicum acetobutylicum]3384 Glucan-glucohydrolase [Microbispora UniRef100_Q59506 MicrobisporaYbbD bispora] bispora 3385 ABC transporter, ATP-binding proteinUniRef100_Q81NK1 Bacillus MntB [Bacillus anthracis] anthracis 3386 ABCtransporter, permease protein UniRef100_Q81NK2 Bacillus MntD [Bacillusanthracis] anthracis 3387 ABC transporter, manganese-bindingUniRef100_Q71YG9 Listeria YcdH protein [Listeria monocytogenes]monocytogenes 3388 Hypothetical protein [Bacillus cereus]UniRef100_Q74NQ1 Bacillus cereus YhjQ 3389 Phosphosugar-bindingtranscriptional UniRef100_Q837Y3 Enterococcus YfiA regulator, RpiRfamily, putative faecalis [Enterococcus faecalis] 33901-phosphofructokinase [Enterococcus UniRef100_Q837Y2 Enterococcus FruKfaecalis] faecalis 3391 PTS system, fructose-specific family,UniRef100_Q837Y1 Enterococcus FruA IIBC components [Enterococcusfaecalis faecalis] 3392 PTS system, IIA component UniRef100_Q837Y0Enterococcus FruA [Enterococcus faecalis] faecalis 3393Fructose/tagatose bisphosphate UniRef100_Q7MC78 Vibrio vulnificus FbaAaldolase [Vibrio vulnificus] 3394 3395 Heavy metal-transporting ATPaseUniRef100_Q6HF81 Bacillus YvgX [Bacillus thuringiensis] thuringiensis3396 Copper chaperone copZ [Bacillus UniRef100_O32221 Bacillus subtilissubtilis] 3397 YvgZ protein [Bacillus subtilis] UniRef100_O32222Bacillus subtilis YvgZ 3398 Intracellular proteinase [ThermoplasmaUniRef100_Q97BN0 Thermoplasma YraA volcanium] volcanium 3399 PutativeHTH-type transcriptional UniRef100_O05261 Bacillus subtilis YulBregulator yulB [Bacillus subtilis] 3400 Rhamnulose kinase/L-fuculosekinase UniRef100_Q8A1A3 Bacteroides YulC [Bacteroides thetaiotaomicron]thetaiotaomicron 3401 Putative sugar isomerase UniRef100_Q9XAB3Streptomyces coelicolor [Streptomyces coelicolor] 3402 Hypotheticalprotein [Polyangium UniRef100_Q9L8B7 Polyangium cellulosum cellulosum]3403 Hypothetical oxidoreductase yuxG UniRef100_P40747 Bacillus subtilisYuxG [Bacillus subtilis] 3404 YfhI protein [Bacillus subtilis]UniRef100_O31577 Bacillus subtilis YfhI 3405 YvmA [Bacillus subtilis]UniRef100_O34307 Bacillus subtilis YvmA 3406 YvmC [Bacillus subtilis]UniRef100_O34351 Bacillus subtilis YvmC 3407 Putative cytochrome P450CYPX UniRef100_O34926 Bacillus subtilis CypX [Bacillus subtilis] 3408Hypothetical protein [Chromobacterium UniRef100_Q7NRR6 Chromobacteriumviolaceum violaceum] 3409 YvnA 3410 Hypothetical protein BRA0099[Brucella UniRef100_Q8FXH8 Brucella suis suis] 3411 Chloroplast Toc64-2[Physcomitrella UniRef100_Q6RJN6 Physcomitrella GatA patens] patens 3412Hypothetical oxidoreductase yvaA UniRef100_O32223 Bacillus subtilis YvaA[Bacillus subtilis] 3413 Putative acyl carrier protein UniRef100_O32224Bacillus subtilis YvaB phosphodiesterase 2 [Bacillus subtilis] 3414Hypothetical UPF0176 protein ybfQ UniRef100_O31457 Bacillus subtilisYbfQ [Bacillus subtilis] 3415 Putative DNA binding responseUniRef100_P94504 Bacillus subtilis regulator, YvrH [Bacillus subtilis]3416 YvrI protein [Bacillus subtilis] UniRef100_O34843 Bacillus subtilisYvrI 3417 3418 Oxalate decarboxylase oxdC [Bacillus UniRef100_O34714Bacillus subtilis OxdC subtilis] 3419 Hypothetical protein yvrL[Bacillus UniRef100_O34686 Bacillus subtilis YvrL subtilis] 3420 BH3120protein [Bacillus halodurans] UniRef100_Q9K886 Bacillus halodurans 34213422 Group-specific protein [Bacillus cereus UniRef100_Q633V1 Bacilluscereus ZK ZK] 3423 3424 3425 3426 UPI00003CC069 UniRef100 entryUniRef100_UPI00003CC069 3427 IS1627s1-related, transposase [BacillusUniRef100_Q7CMD0 Bacillus anthracis str. A2012 anthracis str. A2012]3428 LexA repressor [Listeria UniRef100_Q720B9 Listeria monocytogenesmonocytogenes] 3429 Hypothetical protein [Bacillus cereusUniRef100_Q630E5 Bacillus cereus ZK ZK] 3430 Hypothetical protein[Bacillus anthracis] UniRef100_Q81XX1 Bacillus anthracis 3431 3432Hypothetical protein RSc1705 UniRef100_Q8XYQ3 Ralstonia solanacearum[Ralstonia solanacearum] 3433 YxiD 3434 Hypothetical protein [BacillusUniRef100_Q6HH72 Bacillus thuringiensis thuringiensis] 3435 BlyA 34363437 3438 3439 YomQ protein [Bacteriophage SPBc2] UniRef100_O64053Bacteriophage YomQ SPBc2 3440 XkdV 3441 3442 3443 YubE 3444 ORF38[Bacteriophage phi-105] UniRef100_Q9ZXE5 Bacteriophage phi-105 3445 YqbO3446 Hypothetical protein MW1895 UniRef100_Q8NVQ7 Staphylococcus aureus[Staphylococcus aureus] 3447 3448 3449 Prophage LambdaBa02, major tailUniRef100_Q81W97 Bacillus anthracis protein, putative [Bacillusanthracis] 3450 3451 ORF32 [Bacteriophage phi-105] UniRef100_Q9ZXF1Bacteriophage phi-105 3452 3453 Gp7 protein [Bacteriophage phi3626]UniRef100_Q8SBP7 Bacteriophage phi3626 3454 ORF29 [Bacteriophagephi-105] UniRef100_Q9ZXF4 Bacteriophage phi-105 3455 ORF27[Bacteriophage phi-105] UniRef100_Q9ZXF6 Bacteriophage phi-105 3456 ClpPfamily serine protease, possible UniRef100_Q97HW4 Clostridium ClpP phagerelated [Clostridium acetobutylicum acetobutylicum] 3457 ORF25[Bacteriophage phi-105] UniRef100_Q9ZXF8 Bacteriophage phi-105 3458 3459Putative terminase large subunit UniRef100_Q8SBQ2 Bacteriophage phi3626[Bacteriophage phi3626] 3460 Phage terminase-like protein, smallUniRef100_Q97HW1 Clostridium acetobutylicum subunit [Clostridiumacetobutylicum] 3461 Prophage LambdaBa02, HNH UniRef100_Q81W86 Bacillusanthracis endonuclease family protein [Bacillus anthracis] 3462 Sporecoat protein D [Bacillus subtilis] UniRef100_P07791 Bacillus subtilis3463 ORF16 [Bacteriophage phi-105] UniRef100_Q9ZXB9 Bacteriophagephi-105 3464 3465 3466 ORF14 [Bacteriophage phi-105] UniRef100_Q9ZXC1Bacteriophage phi-105 3467 ORF13 [Bacteriophage phi-105]UniRef100_Q9ZXC2 Bacteriophage phi-105 3468 ORF12 [Bacteriophagephi-105] UniRef100_Q9ZXC3 Bacteriophage phi-105 3469 3470 ORF11[Bacteriophage phi-105] UniRef100_Q9ZXC4 Bacteriophage phi-105 3471ORF10 [Bacteriophage phi-105] UniRef100_Q9ZXC5 Bacteriophage phi-1053472 DNA, complete sequence UniRef100_Q9ZXC7 Bacteriophage phi-105[Bacteriophage phi-105] 3473 DNA, complete sequence UniRef100_Q9ZXC8Bacteriophage phi-105 [Bacteriophage phi-105] 3474 3475 3476 3477 Orf 36[Staphylococcus aureus UniRef100_Q9T1Z5 Staphylococcus aureusbacteriophage PVL] bacteriophage PVL 3478 3479 Orf16 [BacteriophagebIL312] UniRef100_Q9AZE4 Bacteriophage bIL312 3480 Gp26 protein[Bacteriophage phi3626] UniRef100_Q8SBM8 Bacteriophage phi3626 3481 CIlike protein [Lactobacillus casei UniRef100_O64370 Lactobacillus caseibacteriophage A2] bacteriophage A2 3482 Int gene product [StaphylococcusUniRef100_Q932E5 Staphylococcus YdcL aureus] aureus 3483 SsrA-bindingprotein [Bacillus subtilis] UniRef100_O32230 Bacillus subtilis SmpB 3484Ribonuclease R [Bacillus subtilis] UniRef100_O32231 Bacillus subtilisRnr 3485 YvaK protein [Bacillus subtilis] UniRef100_O32232 Bacillussubtilis YvaK 3486 3487 3488 3489 YvaO 3490 YvaO 3491 Glycinebetaine/carnitine/choline UniRef100_O34742 Bacillus subtilis OpuCDtransport system permease protein opuCD [Bacillus subtilis] 3492 Glycinebetaine/carnitine/choline- UniRef100_O32243 Bacillus subtilis OpuCCbinding protein precursor [Bacillus subtilis] 3493 Glycinebetaine/carnitine/choline UniRef100_O34878 Bacillus subtilis OpuCBtransport system permease protein opuCB [Bacillus subtilis] 3494 Glycinebetaine/carnitine/choline UniRef100_O34992 Bacillus subtilis OpuCAtransport ATP-binding protein opuCA [Bacillus subtilis] 3495 ORF-1[Bacillus subtilis] UniRef100_O34709 Bacillus subtilis YvaV 3496 YvbG3497 YvbI 3498 YvbJ protein [Bacillus subtilis] UniRef100_O32247Bacillus subtilis YvbJ 3499 UPI00003CBDCC UniRef100 entryUniRef100_UPI00003CBDCC YfiY 3500 3501 YoaZ [Bacillus subtilis]UniRef100_O34947 Bacillus subtilis YoaZ 3502 Enolase [Bacillus subtilis]UniRef100_P37869 Bacillus subtilis Eno 35032,3-bisphosphoglycerate-independent UniRef100_P39773 Bacillus subtilisPgm phosphoglycerate mutase [Bacillus subtilis] 3504 Triosephosphateisomerase [Bacillus UniRef100_P27876 Bacillus subtilis TpiA subtilis]3505 Phosphoglycerate kinase [Bacillus UniRef100_P40924 Bacillussubtilis Pgk subtilis] 3506 Glyceraldehyde-3-phosphate UniRef100_P09124Bacillus subtilis GapA dehydrogenase 1 [Bacillus subtilis] 3507 Centralglycolytic genes regulator UniRef100_O32253 Bacillus subtilis CggR[Bacillus subtilis] 3508 Hypothetical protein yvbT [BacillusUniRef100_O32254 Bacillus subtilis YvbT subtilis] 3509 YvbW protein[Bacillus subtilis] UniRef100_O32257 Bacillus subtilis YvbW 3510 3511ABC-type multidrug transport system, UniRef100_Q8R7K3 ThermoanaerobacterYfiL ATPase component tengcongensis [Thermoanaerobacter tengcongensis]3512 3513 YvbY protein [Bacillus subtilis] UniRef100_O32259 Bacillussubtilis YvbY 3514 YvfW protein [Bacillus subtilis] UniRef100_O07021Bacillus subtilis YvfW 3515 YvfV 3516 Hypothetical protein yvfI[Bacillus UniRef100_O07007 Bacillus subtilis YvfI subtilis] 3517Putative L-lactate permease yvfH UniRef100_P71067 Bacillus subtilis YvfH[Bacillus subtilis] 3518 RNA polymerase sigma-54 factor UniRef100_P24219Bacillus subtilis SigL [Bacillus subtilis] 3519 Hypothetical proteinyvfG [Bacillus UniRef100_P71066 Bacillus subtilis subtilis] 3520Hypothetical protein yvfF [Bacillus UniRef100_P71065 Bacillus subtilisYvfF subtilis] 3521 YvfE protein [Bacillus subtilis] UniRef100_Q795J3Bacillus subtilis YvfE 3522 Hypothetical protein yvfD [BacillusUniRef100_P71063 Bacillus subtilis YvfD subtilis] 3523 Hypotheticalprotein yvfC [Bacillus UniRef100_P71062 Bacillus subtilis YvfC subtilis]3524 YvfB 3525 YvfA 3526 Hypothetical protein yveT [BacillusUniRef100_P71059 Bacillus subtilis YveT subtilis] 3527 Hypotheticalprotein yveS [Bacillus UniRef100_P71058 Bacillus subtilis YveS subtilis]3528 Hypothetical protein yveR [Bacillus UniRef100_P71057 Bacillussubtilis YveR subtilis] 3529 Hypothetical protein yveQ [BacillusUniRef100_P71056 Bacillus subtilis YveQ subtilis] 3530 Hypotheticalprotein yveP [Bacillus UniRef100_P71055 Bacillus subtilis YveP subtilis]3531 Hypothetical protein yveO [Bacillus UniRef100_P71054 Bacillussubtilis YveO subtilis] 3532 Hypothetical protein yveN [BacillusUniRef100_P71053 Bacillus subtilis YveN subtilis] 3533 Hypotheticalprotein yveM [Bacillus UniRef100_P71052 Bacillus subtilis YveM subtilis]3534 YveL 3535 Chain length determinant protein UniRef100_Q72XH7Bacillus cereus YveK [Bacillus cereus] 3536 Hypothetical protein yveJ[Bacillus UniRef100_P71049 Bacillus subtilis Slr subtilis] 3537 Generalstress protein 14 [Bacillus UniRef100_P80871 Bacillus subtilis YwrOsubtilis] 3538 YcbE 3539 UPI000027D43B UniRef100 entryUniRef100_UPI000027D43B YvcT 3540 4-hydroxythreonine-4-phosphateUniRef100_Q8CUU4 Oceanobacillus iheyensis dehydrogenase (EC 1.1.1.262)(4- (phosphohydroxy)-L-threonine dehydrogenase) [Oceanobacillusiheyensis] 3541 Hypothetical conserved protein UniRef100_Q8CUU5Oceanobacillus iheyensis [Oceanobacillus iheyensis] 3542 GbsB 3543 DapA3544 Transcriptional regulator UniRef100_Q8CUU7 Oceanobacillus RocR[Oceanobacillus iheyensis] iheyensis 3545 Hypothetical protein[Bdellovibrio UniRef100_Q6MH73 Bdellovibrio bacteriovorus bacteriovorus]3546 NADH-dependent flavin oxidoreductase UniRef100_Q8ELB6Oceanobacillus YqiG [Oceanobacillus iheyensis] iheyensis 3547Levansucrase precursor [Bacillus UniRef100_P05655 Bacillus subtilis SacBsubtilis] 3548 Hypothetical protein yveB [Bacillus UniRef100_O07003Bacillus subtilis YveB subtilis] 3549 Hypothetical protein [PseudomonasUniRef100_Q9I5B4 Pseudomonas aeruginosa aeruginosa] 3550 ATP-dependentClp protease UniRef100_P80244 Bacillus subtilis ClpP proteolytic subunit[Bacillus subtilis] 3551 Hypothetical protein yvdD [BacillusUniRef100_O06986 Bacillus subtilis YvdD subtilis] 3552 3553 Possiblemetal-dependent UniRef100_Q6HM73 Bacillus YdhJ phosphohydrolase;possible oxetanocin thuringiensis A resistance protein [Bacillusthuringiensis] 3554 Hypothetical protein yvdB [Bacillus UniRef100_O06984Bacillus subtilis YvdB subtilis] 3555 Hypothetical protein yvdA[Bacillus UniRef100_O06983 Bacillus subtilis YvdA subtilis] 3556Probable 2-ketogluconate reductase UniRef100_O32264 Bacillus subtilisYvcT [Bacillus subtilis] 3557 BH0392 protein [Bacillus halodurans]UniRef100_Q9KFT5 Bacillus YdfE halodurans 3558 BH0391 protein [Bacillushalodurans] UniRef100_Q9KFT6 Bacillus YdfF halodurans 3559 Ribonuclease[Paenibacillus polymyxa] UniRef100_Q7M0X3 Paenibacillus polymyxa 3560Barstar [Bacillus amyloliquefaciens] UniRef100_P11540 Bacillusamyloliquefaciens 3561 Hypothetical protein yvcN [BacillusUniRef100_O06977 Bacillus subtilis YvcN subtilis] 3562 HPr-like proteincrh [Bacillus subtilis] UniRef100_O06976 Bacillus subtilis 3563Hypothetical protein yvcL [Bacillus UniRef100_O06975 Bacillus subtilisYvcL subtilis] 3564 Hypothetical UPF0052 protein yvcK UniRef100_O06974Bacillus subtilis YvcK [Bacillus subtilis] 3565 Hypothetical UPF0042protein yvcJ UniRef100_O06973 Bacillus subtilis YvcJ [Bacillus subtilis]3566 Hypothetical protein yvcI [Bacillus UniRef100_O06972 Bacillussubtilis YvcI subtilis] 3567 Thioredoxin reductase [Bacillus subtilis]UniRef100_P80880 Bacillus subtilis TrxB 3568 Hypothetical protein yvcD[Bacillus UniRef100_O06968 Bacillus subtilis YvcD subtilis] 3569Hypothetical conserved protein UniRef100_Q8ETL1 Oceanobacillus YbbJ[Oceanobacillus iheyensis] iheyensis 3570 Histidine biosynthesisbifunctional UniRef100_O34912 Includes: HisI protein hisIE [Includes:Phosphoribosyl- Phosphoribosyl- AMP cyclohydrolase (EC 3.5.4.19) AMP(PRA-CH); Phosphoribosyl-ATP cyclohydrolase pyrophosphatase (EC3.6.1.31) (PRA- (EC 3.5.4.19) PH)] [Bacillus subtilis] (PRA-CH);Phosphoribosyl- ATP pyrophosphatase (EC 3.6.1.31) (PRA-PH) 3571Imidazole glycerol phosphate synthase UniRef100_O34727 Bacillus subtilisHisF subunit hisF [Bacillus subtilis] 3572 1-(5-phosphoribosyl)-5-[(5-UniRef100_O35006 (5- HisA phosphoribosylamino)methylideneamino]phosphoribosylamino)methylideneamino imidazole-4-carboxamide isomerase[Bacillus subtilis] 3573 Imidazole glycerol phosphate synthaseUniRef100_O34565 Bacillus subtilis HisH subunit hisH [Bacillus subtilis]3574 Imidazoleglycerol-phosphate UniRef100_O34683 Bacillus subtilis HisBdehydratase [Bacillus subtilis] 3575 Histidinol dehydrogenase [BacillusUniRef100_O34651 Bacillus subtilis HisD subtilis] 3576 ATPphosphoribosyltransferase UniRef100_O34520 Bacillus subtilis HisG[Bacillus subtilis] 3577 HisZ 3578 YvpB [Bacillus subtilis]UniRef100_O34735 Bacillus subtilis YvpB 3579 Pectate lyase Pel-28K[Bacillus sp. P- UniRef100_Q8L0R5 Bacillus sp. P- YvpA 2850] 2850 3580Putative acetyltransferase [Bacillus UniRef100_O34993 Bacillus subtilisYvoF subtilis] 3581 Pyrophosphatase ppaX [Bacillus UniRef100_Q9JMQ2Bacillus subtilis HprP subtilis] 3582 YvoD [Bacillus subtilis]UniRef100_O34382 Bacillus subtilis YvoD 3583 Prolipoproteindiacylglyceryl transferase UniRef100_O34752 Bacillus subtilis Lgt[Bacillus subtilis] 3584 HPr kinase/phosphorylase (EC 2.7.1.—)UniRef100_O34483 Bacillus subtilis HprK (EC 2.7.4.—) (HPrK/P) (HPr(Ser)kinase/phosphorylase) [Bacillus subtilis] 3585 YfiV protein [Bacillussubtilis] UniRef100_O31564 Bacillus subtilis YfiV 3586 YfiU protein[Bacillus subtilis] UniRef100_O31563 Bacillus subtilis YfiU 3587 YvnB[Bacillus subtilis] UniRef100_O34986 Bacillus subtilis YvnB 3588 YvlD[Bacillus subtilis] UniRef100_O34648 Bacillus subtilis YvlD 3589 YvlC[Bacillus subtilis] UniRef100_O34719 Bacillus subtilis 3590 YvlB[Bacillus subtilis] UniRef100_O34628 Bacillus subtilis YvlB 3591Hypothetical protein yvlA [Bacillus UniRef100_O34322 Bacillus subtilisYvlA subtilis] 3592 Hypothetical protein yvkN [Bacillus UniRef100_O34604Bacillus subtilis subtilis] 3593 3594 UvrABC system protein A [BacillusUniRef100_O34863 Bacillus subtilis UvrA subtilis] 3595 UvrABC systemprotein B [Bacillus UniRef100_Q659H3 Bacillus UvrB amyloliquefaciens]amyloliquefaciens 3596 CsbA protein [Bacillus subtilis] UniRef100_P37953Bacillus subtilis 3597 3598 YvjD [Bacillus subtilis] UniRef100_O34375Bacillus subtilis YvjD 3599 YvzD 3600 Putative protease [Bacillussubtilis] UniRef100_O35002 Bacillus subtilis YvjB 3601 Tartratedehydrogenase UniRef100_Q8ESH5 Oceanobacillus YcsA [Oceanobacillusiheyensis] iheyensis 3602 Cell wall-binding protein [BacillusUniRef100_Q9K6X4 Bacillus YvcE halodurans] halodurans 3603 Cell divisionprotein ftsX homolog UniRef100_O34876 Bacillus subtilis FtsX [Bacillussubtilis] 3604 Cell division ATP-binding protein UniRef100_O34814Bacillus subtilis FtsE [Bacillus subtilis] 3605 Cytochrome c-551[Bacillus subtilis] UniRef100_O34594 Bacillus subtilis CccB 3606 YvjA[Bacillus subtilis] UniRef100_O34792 Bacillus subtilis YvjA 3607 Peptidechain release factor 2 [Bacillus UniRef100_P28367 Bacillus subtilis PrfBsubtilis] 3608 Preprotein translocase secA subunit UniRef100_P28366Bacillus subtilis SecA [Bacillus subtilis] 3609 Hypothetical proteinyvyD [Bacillus UniRef100_P28368 Bacillus subtilis YvyD subtilis] 36103611 Flagellar protein fliT [Bacillus subtilis] UniRef100_P39740Bacillus subtilis FliT 3612 Flagellar protein fliS [Bacillus subtilis]UniRef100_P39739 Bacillus subtilis FliS 3613 Flagellar hook-associatedprotein 2 UniRef100_P39738 Bacillus subtilis FliD [Bacillus subtilis]3614 Hypothetical protein yvyC [Bacillus UniRef100_P39737 Bacillussubtilis YvyC subtilis] 3615 Flagellin [Bacillus subtilis]UniRef100_P02968 Bacillus subtilis Hag 3616 Carbon storage regulatorhomolog UniRef100_P33911 Bacillus subtilis [Bacillus subtilis] 3617Transmembrane protein [Bacillus UniRef100_P96503 Bacillus subtilis YviFsubtilis] 3618 YviE protein [Bacillus subtilis] UniRef100_P96502Bacillus subtilis YviE 3619 Flagellar hook-associated protein 3UniRef100_P96501 Bacillus subtilis FlgL [Bacillus subtilis] 3620Flagellar hook-associated protein 1 UniRef100_P39810 Bacillus subtilisFlgK [Bacillus subtilis] 3621 Hypothetical protein yvyG [BacillusUniRef100_P39808 Bacillus subtilis YvyG subtilis] 3622 Negativeregulator of flagellin synthesis UniRef100_P39809 Bacillus subtilis[Bacillus subtilis] 3623 Hypothetical protein yvyF [BacillusUniRef100_P39807 Bacillus subtilis YvyF subtilis] 3624 ComFC 3625 3626ComFA 3627 DegV protein [Bacillus subtilis] UniRef100_P32436 Bacillussubtilis YviA 3628 Transcriptional regulatory protein degUUniRef100_P13800 Bacillus subtilis DegU [Bacillus subtilis] 3629 DegS3630 Hypothetical UPF0029 protein yvyE UniRef100_P32437 Bacillussubtilis YvyE [Bacillus subtilis] 3631 Putative transcriptionalregulator UniRef100_P96499 Bacillus subtilis YvhJ [Bacillus subtilis]3632 Hypothetical protein yunG [Bacillus UniRef100_Q63CU2 Bacilluscereus YunG cereus ZK] ZK 3633 Probable undecaprenyl-phosphate N-UniRef100_O34753 Bacillus subtilis TagO acetylglucosaminyl 1-phosphatetransferase [Bacillus subtilis] 3634 Putative teichuronic acidbiosynthesis UniRef100_O32267 Bacillus subtilis TuaH glycosyltransferase tuaH [Bacillus subtilis] 3635 Putative teichuronic acidbiosynthesis UniRef100_O32268 Bacillus subtilis TuaG glycosyltransferase tuaG [Bacillus subtilis] 3636 Teichuronic acid biosynthesisprotein UniRef100_O32269 Bacillus subtilis TuaF tuaF [Bacillus subtilis]3637 Teichuronic acid biosynthesis protein UniRef100_O32270 Bacillussubtilis TuaE tuaE [Bacillus subtilis] 3638 Putative UDP-glucose4-epimerase UniRef100_Q57664 Methanococcus GalE [Methanococcusjannaschii] jannaschii 3639 UDP-glucose 6-dehydrogenase UniRef100_O32271Bacillus subtilis TuaD [Bacillus subtilis] 3640 Putative teichuronicacid biosynthesis UniRef100_O32272 Bacillus subtilis TuaC glycosyltransferase tuaC [Bacillus subtilis] 3641 Teichuronic acid biosynthesisprotein UniRef100_O32273 Bacillus subtilis TuaB tuaB [Bacillus subtilis]3642 UPI00003CB85E UniRef100 entry UniRef100_UPI00003CB85E TuaA 3643LytC 3644 Amidase enhancer precursor [Bacillus UniRef100_Q02113 Bacillussubtilis LytB subtilis] 3645 LytA 3646 Membrane-bound protein lytR[Bacillus UniRef100_Q02115 Bacillus subtilis LytR subtilis] 3647UDP-N-acetylglucosamine 2-epimerase UniRef100_P39131 Bacillus subtilisYvyH [Bacillus subtilis] 3648 UTP--glucose-1-phosphate UniRef100_Q05852Bacillus subtilis GtaB uridylyltransferase [Bacillus subtilis] 3649 TagH3650 Teichoic acid translocation permease UniRef100_P42953 Bacillussubtilis TagG protein tagG [Bacillus subtilis] 3651 Teichoic acidbiosynthesis protein UniRef100_Q9CH20 Lactococcus TagF [Lactococcuslactis] lactis 3652 CDP-glycerol:poly(glycerophosphate) UniRef100_P13485Bacillus subtilis TagF glycerophosphotransferase [Bacillus subtilis]3653 TagD 3654 TagA 3655 Putative CDP- UniRef100_P27621 Bacillussubtilis TagB glycerol:glycerophosphate glycerophosphotransferase[Bacillus subtilis] 3656 Beta-N-acetylglucosaminidase UniRef100_P39848Bacillus subtilis LytD precursor [Bacillus subtilis] 3657Mannose-6-phosphate isomerase UniRef100_P39841 Bacillus subtilis Pmi[Bacillus subtilis] 3658 3659 Anaerobic ribonucleoside-triphosphateUniRef100_Q733N6 Bacillus cereus reductase, putative [Bacillus cereus]3660 2-keto-3-deoxygluconate permease UniRef100_P50847 Bacillus subtilisKdgT [Bacillus subtilis] 3661 KHG/KDPG aldolase [Includes: 4-UniRef100_P50846 Includes: 4- KdgA hydroxy-2-oxoglutarate aldolase (EChydroxy-2- 4.1.3.16) (2-keto-4-hydroxyglutarate oxoglutarate aldolase)(KHG-aldolase); 2-dehydro- aldolase (EC 3-deoxy-phosphogluconatealdolase 4.1.3.16) (2-keto- (EC 4.1.2.14) (Phospho-2-dehydro-3- 4-deoxygluconate aldolase) (Phospho-2- hydroxyglutarate keto-3-d aldolase)(KHG- aldolase); 2- dehydro-3- deoxy- phosphogluconate aldolase (EC4.1.2.14) (Phospho-2- dehydro-3- deoxygluconate aldolase)(Phospho-2-keto- 3- deoxygluconate aldol 36622-dehydro-3-deoxygluconokinase UniRef100_P50845 Bacillus subtilis KdgK[Bacillus subtilis] 3663 HTH-type transcriptional regulator kdgRUniRef100_P50844 Bacillus subtilis KdgR [Bacillus subtilis] 3664 KduI3665 2-deoxy-D-gluconate 3-dehydrogenase UniRef100_P50842 Bacillussubtilis KduD [Bacillus subtilis] 3666 Alcohol dehydrogenase,glutathione- UniRef100_Q6HGX1 Bacillus AdhB dependent formaldehydethuringiensis dehydrogenase [Bacillus thuringiensis] 3667 YwtG protein[Bacillus subtilis] UniRef100_P96742 Bacillus subtilis YwtG 3668Hypothetical transcriptional regulator UniRef100_Q7WY78 Bacillussubtilis YwtF ywtF [Bacillus subtilis] 3669 YwtE protein [Bacillussubtilis] UniRef100_P96741 Bacillus subtilis YwtE 3670 Gamma-DL-glutamylhydrolase UniRef100_P96740 Bacillus subtilis YwtD precursor [Bacillussubtilis] 3671 3672 YwtB protein [Bacillus subtilis] UniRef100_P96738Bacillus subtilis YwtB 3673 YwtA protein [Bacillus subtilis]UniRef100_P96737 Bacillus subtilis YwtA 3674 YwsC protein [Bacillussubtilis] UniRef100_P96736 Bacillus subtilis YwsC 3675 RbsR 3676Ribokinase [Bacillus subtilis] UniRef100_P36945 Bacillus subtilis RbsK3677 High affinity ribose transport protein UniRef100_P36946 Bacillussubtilis RbsD rbsD [Bacillus subtilis] 3678 Ribose transport ATP-bindingprotein UniRef100_P36947 Bacillus subtilis RbsA rbsA [Bacillus subtilis]3679 Ribose transport system permease UniRef100_P36948 Bacillus subtilisRbsC protein rbsC [Bacillus subtilis] 3680 D-ribose-binding proteinprecursor UniRef100_P36949 Bacillus subtilis RbsB [Bacillus subtilis]3681 Alpha-acetolactate decarboxylase UniRef100_Q04777 Bacillus subtilisAlsD [Bacillus subtilis] 3682 Acetolactate synthase [Bacillus subtilis]UniRef100_Q04789 Bacillus subtilis AlsS 3683 HTH-type transcriptionalregulator alsR UniRef100_Q04778 Bacillus subtilis AlsR [Bacillussubtilis] 3684 YwrD protein [Bacillus subtilis] UniRef100_O05218Bacillus subtilis YwrD 3685 YwrC protein [Bacillus subtilis]UniRef100_O05217 Bacillus subtilis YwrC 3686 YwrB protein [Bacillussubtilis] UniRef100_O05216 Bacillus subtilis YwrB 3687 YwrA protein[Bacillus subtilis] UniRef100_O05215 Bacillus subtilis YwrA 3688Putative protein-tyrosine phosphatase UniRef100_P96717 Bacillus subtilisYwqE ywqE [Bacillus subtilis] 3689 Hypothetical protein ywqC [BacillusUniRef100_P96715 Bacillus subtilis YwqC subtilis] 3690 Hypotheticalprotein ywqB [Bacillus UniRef100_P96714 Bacillus subtilis YwqB subtilis]3691 YwqA protein [Bacillus subtilis] UniRef100_P94593 Bacillus subtilisYwqA 3692 YwpJ protein [Bacillus subtilis] UniRef100_P94592 Bacillussubtilis YwpJ 3693 YwpI protein [Bacillus subtilis] UniRef100_P94591Bacillus subtilis GlcR 3694 Fructose-6-phosphate aldolase 2UniRef100_P58424 Escherichia coli YwjH [Escherichia coli O157:H7]O157:H7 3695 Hypothetical protein PM1968 UniRef100_Q9CJM9 PasteurellaFabG [Pasteurella multocida] multocida 3696 PTS system,sorbitol-specific IIA UniRef100_Q82YX6 Enterococcus faecalis component[Enterococcus faecalis] 3697 PTS system, sorbitol-specific IIBCUniRef100_Q82YX5 Enterococcus faecalis components [Enterococcusfaecalis] 3698 UPI00002F4A3A UniRef100 entry UniRef100_UPI00002F4A3A3699 Sorbitol operon activator UniRef100_Q9X673 Streptococcus mutans[Streptococcus mutans] 3700 Putative transcriptional regulator ofUniRef100_Q83PA8 Shigella flexneri DeoR sorbose uptake and utilizationgenes [Shigella flexneri] 3701 YwpH protein [Bacillus subtilis]UniRef100_P94590 Bacillus subtilis YwpH 3702 Hypothetical protein ywpG[Bacillus UniRef100_P94589 Bacillus subtilis YwpG subtilis] 3703 YwpFprotein [Bacillus subtilis] UniRef100_P94588 Bacillus subtilis YwpF 3704Large-conductance mechanosensitive UniRef100_P94585 Bacillus subtilisMscL channel [Bacillus subtilis] 3705 (3R)-hydroxymyristoyl-[acylcarrier UniRef100_Q81JE0 acyl carrier YwpB protein] dehydratase (EC4.2.1.—) ((3R)- protein hydroxymyristoyl ACP dehydrase) [Bacillusanthracis] 3706 Flagellar hook-basal body complex UniRef100_P39753Bacillus subtilis FlhP protein flhP [Bacillus subtilis] 3707 Flagellarhook-basal body complex UniRef100_P39752 Bacillus subtilis FlhO proteinflhO [Bacillus subtilis] 3708 MreB-like protein [Bacillus subtilis]UniRef100_P39751 Bacillus subtilis MbI 3709 Stage III sporulationprotein D [Bacillus UniRef100_P15281 Bacillus subtilis subtilis] 37103711 Hypothetical protein ywoH [Bacillus UniRef100_P94578 Bacillussubtilis YwoH subtilis] 3712 Hypothetical protein ywoG [BacillusUniRef100_P94577 Bacillus subtilis YwoG subtilis] 3713 Hypotheticalprotein ywoA [Bacillus UniRef100_P94571 Bacillus subtilis YwoA subtilis]3714 Manganese-dependent inorganic UniRef100_Q63AC7 Bacillus cereus PpaCpyrophosphatase [Bacillus cereus ZK] ZK 3715 Probable manganese catalase[Bacillus UniRef100_P80878 Bacillus subtilis YdbD subtilis] 3716Hypothetical protein yqjF [Bacillus UniRef100_P54543 Bacillus subtilisYqjF subtilis] 3717 BH2605 protein [Bacillus halodurans]UniRef100_Q9K9P0 Bacillus YhcV halodurans 3718 Hypothetical protein yqxL[Bacillus UniRef100_P40948 Bacillus subtilis YqxL subtilis] 3719Hypothetical UPF0053 protein yqhB UniRef100_P54505 Bacillus subtilisYqhB [Bacillus subtilis] 3720 Ammonium transporter nrgA [BacillusUniRef100_Q07429 Bacillus subtilis NrgA subtilis] 3721 Nitrogenregulatory PII-like protein UniRef100_Q07428 Bacillus subtilis NrgB[Bacillus subtilis] 3722 ATP-dependent Clp protease UniRef100_Q81CH1Bacillus cereus ClpP proteolytic subunit [Bacillus cereus] 3723 RNApolymerase ECF-type sigma UniRef100_Q63AF9 Bacillus cereus SigM factor[Bacillus cereus ZK] ZK 3724 Hypothetical protein ywnI [BacillusUniRef100_P71044 Bacillus subtilis SpoIIQ subtilis] 3725 Hypotheticalprotein ywnH [Bacillus UniRef100_P71043 Bacillus subtilis YwnH subtilis]3726 Hypothetical protein ywnF [Bacillus UniRef100_P71041 Bacillussubtilis YwnF subtilis] 3727 Probable cardiolipin synthetase 2UniRef100_P71040 Bacillus subtilis YwnE [Bacillus subtilis] 3728UPI00003CB764 UniRef100 entry UniRef100_UPI00003CB764 YhcA 3729Antibiotic/siderophore biosynthesis UniRef100_Q81DP7 Bacillus cereusprotein [Bacillus cereus] 3730 Probable serine activating enzymeUniRef100_P45745 Bacillus subtilis DhbF [Bacillus subtilis] 3731Isochorismatase [Bacillus subtilis] UniRef100_P45743 Bacillus subtilisDhbB 3732 2,3-dihydroxybenzoate-AMP ligase UniRef100_P40871 Bacillussubtilis DhbE [Bacillus subtilis] 3733 Isochorismate synthase dhbC[Bacillus UniRef100_P45744 Bacillus subtilis DhbC subtilis] 37342,3-dihydro-2,3-dihydroxybenzoate UniRef100_P39071 Bacillus subtilisDhbA dehydrogenase [Bacillus subtilis] 3735 YuiI protein [Bacillussubtilis] UniRef100_O32102 Bacillus subtilis YuiI 3736 Iron-uptakesystem permease protein UniRef100_P40411 Bacillus subtilis FeuC feuC[Bacillus subtilis] 3737 Iron-uptake system permease proteinUniRef100_P40410 Bacillus subtilis FeuB feuB [Bacillus subtilis] 3738Iron-uptake system binding protein UniRef100_P40409 Bacillus subtilisFeuA precursor [Bacillus subtilis] 3739 Putative HTH-typetranscriptional UniRef100_P40408 Bacillus subtilis YbbB regulator ybbB[Bacillus subtilis] 3740 Hypothetical protein ywnG [BacillusUniRef100_P71042 Bacillus subtilis YwnC subtilis] 3741 UPI00003CC069UniRef100 entry UniRef100_UPI00003CC069 3742 IS1627s1-related,transposase [Bacillus UniRef100_Q7CMD0 Bacillus anthracis str. A2012anthracis str. A2012] 3743 Hypothetical protein ywnB [BacillusUniRef100_P71037 Bacillus subtilis YwnB subtilis] 3744 Hypotheticalprotein ywnA [Bacillus UniRef100_P71036 Bacillus subtilis YwnA subtilis]3745 3746 Hypothetical protein ywmF [Bacillus UniRef100_P70963 Bacillussubtilis YwmF subtilis] 3747 MoaA 3748 FdhD protein homolog [Bacillussubtilis] UniRef100_P39756 Bacillus subtilis FdhD 3749 BH2528 protein[Bacillus halodurans] UniRef100_Q9K9W7 Bacillus YbfB halodurans 3750Formate dehydrogenase alpha chain UniRef100_Q9K9W5 Bacillus YjgC[Bacillus halodurans] halodurans 3751 YwmD 3752 Hypothetical proteinywmC precursor UniRef100_P70960 Bacillus subtilis YwmC [Bacillussubtilis] 3753 Stage II sporulation protein D [Bacillus UniRef100_P07372Bacillus subtilis SpoIID subtilis] 3754 UDP-N-acetylglucosamine 1-UniRef100_P70965 Bacillus subtilis MurAA carboxyvinyltransferase 1[Bacillus subtilis] 3755 YwmB protein [Bacillus subtilis]UniRef100_O32277 Bacillus subtilis YwmB 3756 YwzB protein [Bacillussubtilis] UniRef100_O32278 Bacillus subtilis 3757 ATP synthase epsilonchain [Bacillus UniRef100_P37812 Bacillus subtilis AtpC subtilis] 3758ATP synthase beta chain [Bacillus UniRef100_P37809 Bacillus subtilisAtpD subtilis] 3759 ATP synthase gamma chain [Bacillus UniRef100_P37810Bacillus subtilis AtpG subtilis] 3760 ATP synthase alpha chain [BacillusUniRef100_Q659H2 Bacillus AtpA amyloliquefaciens] amyloliquefaciens 3761ATP synthase delta chain [Bacillus UniRef100_P37811 Bacillus subtilisAtpH subtilis] 3762 ATP synthase B chain [Bacillus subtilis]UniRef100_P37814 Bacillus subtilis AtpF 3763 ATP synthase a chain[Bacillus subtilis] UniRef100_P37813 Bacillus subtilis AtpB 3764 AtpI3765 Uracil phosphoribosyltransferase UniRef100_P39149 Bacillus subtilisUpp [Bacillus subtilis] 3766 Serine hydroxymethyltransferaseUniRef100_P39148 Bacillus subtilis GlyA [Bacillus subtilis] 3767Hypothetical protein ywlG [Bacillus UniRef100_P39157 Bacillus subtilisYwlG subtilis] 3768 Hypothetical lacA/rpiB family proteinUniRef100_P39156 Bacillus subtilis YwlF ywlF [Bacillus subtilis] 3769Putative low molecular weight protein- UniRef100_P39155 Bacillussubtilis YwlE tyrosine-phosphatase ywlE [Bacillus subtilis] 3770Hypothetical UPF0059 protein ywlD UniRef100_P39154 Bacillus subtilisYwlD [Bacillus subtilis] 3771 Hypothetical protein ywlC [BacillusUniRef100_P39153 Bacillus subtilis YwlC subtilis] 3772 Hypotheticalprotein ywlB [Bacillus UniRef100_P39152 Bacillus subtilis YwlB subtilis]3773 Stage II sporulation protein R [Bacillus UniRef100_P39151 Bacillussubtilis SpoIIR subtilis] 3774 Hypothetical protein yhjI [BacillusUniRef100_O07563 Bacillus subtilis GlcP subtilis] 3775 Hypotheticalprotein yhjJ [Bacillus UniRef100_O07564 Bacillus subtilis YhjJ subtilis]3776 Hypothetical protein [Bacillus anthracis] UniRef100_Q81NC8 BacillusYhjK anthracis 3777 Hypothetical protein yhjL [Bacillus UniRef100_O07566Bacillus subtilis YhjL subtilis] 3778 Hypothetical protein yhjM[Bacillus UniRef100_O07567 Bacillus subtilis YhjM subtilis] 3779 HemKprotein homolog [Bacillus UniRef100_P45873 Bacillus subtilis YwkEsubtilis] 3780 Peptide chain release factor 1 [Bacillus UniRef100_P45872Bacillus subtilis PrfA subtilis] 3781 Hypothetical protein ywkD[Bacillus UniRef100_P45871 Bacillus subtilis YwkD subtilis] 3782Hypothetical protein ywkC [Bacillus UniRef100_P45870 Bacillus subtilisYwkC subtilis] 3783 Hypothetical protein ywkB [Bacillus UniRef100_P45869Bacillus subtilis YwkB subtilis] 3784 Probable NAD-dependent malicUniRef100_P45868 Bacillus subtilis YwkA enzyme 2 [Bacillus subtilis]3785 Thymidine kinase [Bacillus subtilis] UniRef100_Q03221 Bacillussubtilis Tdk 3786 50S ribosomal protein L31 [Bacillus UniRef100_Q03223Bacillus subtilis subtilis] 3787 Transcription termination factor rhoUniRef100_Q03222 Bacillus subtilis Rho [Bacillus subtilis] 3788Hypothetical protein ywjI [Bacillus UniRef100_Q03224 Bacillus subtilisYwjI subtilis] 3789 UDP-N-acetylglucosamine 1- UniRef100_P19670 Bacillussubtilis MurAB carboxyvinyltransferase 2 [Bacillus subtilis] 3790Transaldolase [Bacillus subtilis] UniRef100_P19669 Bacillus subtilisYwjH 3791 Probable fructose-bisphosphate UniRef100_P13243 Bacillussubtilis FbaA aldolase 1 [Bacillus subtilis] 3792 Sporulation initiationUniRef100_P06628 Bacillus subtilis Spo0F phosphotransferase F [Bacillussubtilis] 3793 Hypothetical protein ywjG [Bacillus UniRef100_P06629Bacillus subtilis YwjG subtilis] 3794 PyrG 3795 DNA-directed RNApolymerase delta UniRef100_P12464 Bacillus subtilis RpoE subunit[Bacillus subtilis] 3796 Acyl-CoA dehydrogenase [BacillusUniRef100_Q814S9 Bacillus cereus AcdA cereus] 3797 Acyl-CoAdehydrogenase [Bacillus UniRef100_Q81JV7 Bacillus MmgC anthracis]anthracis 3798 UPI00003CC303 UniRef100 entry UniRef100_UPI00003CC303MmgB 3799 Acetyl-CoA acetyltransferase UniRef100_Q8EM47 OceanobacillusMmgA [Oceanobacillus iheyensis] iheyensis 3800 Hypothetical protein ywjF[Bacillus UniRef100_P45866 Bacillus subtilis YwjF subtilis] 3801 YwjE3802 Hypothetical protein ywjC [Bacillus UniRef100_P45863 Bacillussubtilis subtilis] 3803 Arginyl-tRNA synthetase [BacillusUniRef100_P46906 Bacillus subtilis ArgS subtilis] 3804 YwiB 3805Agmatinase [Bacillus subtilis] UniRef100_P70999 Bacillus subtilis SpeB3806 Spermidine synthase [Bacillus subtilis] UniRef100_P70998 Bacillussubtilis SpeE 3807 UPI00002A1DD1 UniRef100 entry UniRef100_UPI00002A1DD13808 Hypothetical protein ywhE [Bacillus UniRef100_P70997 Bacillussubtilis YwhE subtilis] 3809 Hypothetical protein ywhD [BacillusUniRef100_P70996 Bacillus subtilis YwhD subtilis] 3810 Hypotheticalprotein ywhC [Bacillus UniRef100_P70995 Bacillus subtilis YwhC subtilis]3811 Probable tautomerase ywhB [Bacillus UniRef100_P70994 Bacillussubtilis subtilis] 3812 Conserved protein [MethanosarcinaUniRef100_Q8PUC6 Methanosarcina mazei mazei] 3813 Hypothetical proteinywgA [Bacillus UniRef100_P71046 Bacillus subtilis YwgA subtilis] 3814Hypothetical protein ywfO [Bacillus UniRef100_P39651 Bacillus subtilisYwfO subtilis] 3815 YwzC protein [Bacillus subtilis] UniRef100_O32280Bacillus subtilis 3816 Prespore specific transcriptionalUniRef100_P39650 Bacillus subtilis RsfA regulator rsfA [Bacillussubtilis] 3817 Hypothetical protein yjdJ precursor UniRef100_O31651Bacillus subtilis [Bacillus subtilis] 3818 Hypothetical protein ywfL[Bacillus UniRef100_P39648 Bacillus subtilis YwfL subtilis] 38194-hydroxybenzoate 3-monooxygenase UniRef100_Q59724 Pseudomonas sp YhjG[Pseudomonas sp] 3820 Benzoate transporter protein UniRef100_Q6XUN5Pseudomonas YceI [Pseudomonas sp. ND6] sp. ND6 3821 Putative3,4-dihydroxyphenylacetate UniRef100_Q6N986 Rhodopseudomonas palustris2,3-dioxygenase [Rhodopseudomonas palustris] 3822 4-oxalocrotonatetautomerase UniRef100_Q9Z431 Pseudomonas putida [Pseudomonas putida]3823 4-oxalocrotonate decarboxylase UniRef100_Q84I96 Pseudomonas sp.S-47 [Pseudomonas sp. S-47] 3824 DhaS 3825 Putative transcriptionalregulator UniRef100_Q9F7C7 Agrobacterium KipR [Agrobacteriumtumefaciens] tumefaciens 3826 Hypothetical protein SCO6305UniRef100_Q93RT6 Streptomyces coelicolor [Streptomyces coelicolor] 3827Phosphate acetyltransferase [Bacillus UniRef100_P39646 Bacillus subtilisPta subtilis] 3828 Hypothetical protein ywfI [Bacillus UniRef100_P39645Bacillus subtilis YwfI subtilis] 3829 YuaB 3830 Na+-transporting ATPsynthase UniRef100_Q9KF87 Bacillus YubG [Bacillus halodurans] halodurans3831 Hypothetical protein ywdL [Bacillus UniRef100_P39620 Bacillussubtilis YwdL subtilis] 3832 Multidrug ABC transporter, permeaseUniRef100_Q63D13 Bacillus cereus YfiN [Bacillus cereus ZK] ZK 3833 ABCtransporter, permease protein UniRef100_Q73AB4 Bacillus cereus YfiM[Bacillus cereus] 3834 Multidrug ABC transporter, ATP-bindingUniRef100_Q63D15 Bacillus cereus YfiL protein [Bacillus cereus ZK] ZK3835 Sensor histidine kinase, putative UniRef100_Q73AB6 Bacillus cereusYfiJ [Bacillus cereus] 3836 DNA-binding response regulator, LuxRUniRef100_Q73AB7 Bacillus cereus YfiK family [Bacillus cereus] 3837 YwdK3838 Putative purine permease ywdJ UniRef100_P39618 Bacillus subtilisYwdJ [Bacillus subtilis] 3839 Hypothetical protein ywdI [BacillusUniRef100_P39617 Bacillus subtilis YwdI subtilis] 3840 Probable aldehydedehydrogenase UniRef100_P39616 Bacillus subtilis YwdH ywdH [Bacillussubtilis] 3841 Uracil-DNA glycosylase [Bacillus UniRef100_P39615Bacillus subtilis Ung subtilis] 3842 YwdF 3843 Phosphomethylpyrimidinekinase UniRef100_P39610 Bacillus subtilis ThiD [Bacillus subtilis] 3844Hypothetical protein ywdA [Bacillus UniRef100_P39609 Bacillus subtilissubtilis] 3845 Sucrose-6-phosphate hydrolase UniRef100_P07819 Bacillussubtilis SacA [Bacillus subtilis] 3846 PTS system, sucrose-specific IIBCUniRef100_P05306 Bacillus subtilis SacP component [Bacillus subtilis]3847 SacT 3848 3849 Minor extracellular protease vpr UniRef100_P29141Bacillus subtilis Vpr precursor [Bacillus subtilis] 3850 3851 YwcH 3852Nitro/flavin reductase [Bacillus subtilis] UniRef100_P39605 Bacillussubtilis NfrA 3853 N-acyl-L-amino acid amidohydrolase UniRef100_Q9KCF8Bacillus YhaA [Bacillus halodurans] halodurans 3854 Hypothetical proteinywcF [Bacillus UniRef100_P39604 Bacillus subtilis RodA subtilis] 3855RodA 3856 Membrane protein [Clostridium tetani] UniRef100_Q897A1Clostridium tetani 3857 Hypothetical protein ywcE precursorUniRef100_P39603 Bacillus subtilis [Bacillus subtilis] 3858 Clostripain[Methanosarcina UniRef100_Q8TIY6 Methanosarcina acetivorans acetivorans]3859 YfmK protein [Bacillus subtilis] UniRef100_O34536 Bacillus subtilisYfmK 3860 ABC transporter, ATP- UniRef100_Q882Y2 Pseudomonas YgaDbinding/permease protein syringae [Pseudomonas syringae] 3861 HlyB/MsbAfamily ABC transporter UniRef100_Q7NIB9 Gloeobacter YwjA [Gloeobacterviolaceus] violaceus 3862 3863 3864 3865 3866 Response regulator[Bacillus cereus UniRef100_Q63FE4 Bacillus cereus ComA ZK] ZK 3867 3868Quinol oxidase polypeptide IV [Bacillus UniRef100_P34959 Bacillussubtilis QoxD subtilis] 3869 Quinol oxidase polypeptide III (ECUniRef100_P34958 Bacillus subtilis QoxC 1.10.3.—) (Quinol oxidaseaa3-600, subunit qoxC) (Oxidase aa(3)-600 subunit 3) [Bacillus subtilis]3870 Quinol oxidase polypeptide I (EC UniRef100_P34956 Bacillus subtilisQoxB 1.10.3.—) (Quinol oxidase aa3-600, subunit qoxB) (Oxidase aa(3)-600subunit 1) [Bacillus subtilis] 3871 QoxA 3872 UDP-glucose 4-epimeraseUniRef100_Q8R8R8 Thermoanaerobacter GalE [Thermoanaerobactertengcongensis] tengcongensis 3873 3874 3875 3876 Hypothetical proteinywqN [Bacillus UniRef100_P96726 Bacillus subtilis YwqN subtilis] 3877Hypothetical protein ywcB [Bacillus UniRef100_P39600 Bacillus subtilissubtilis] 3878 Putative symporter ywcA [Bacillus UniRef100_P39599Bacillus subtilis YwcA subtilis] 3879 XylB 3880 Xylose isomerase[Bacillus sp.] UniRef100_P54272 Bacillus sp. XylA 3881 XylR 3882Thiamine-phosphate UniRef100_P39594 Bacillus subtilis ThiEpyrophosphorylase [Bacillus subtilis] 3883 Hydroxyethylthiazole kinase[Bacillus UniRef100_P39593 Bacillus subtilis ThiM subtilis] 3884Putative HTH-type transcriptional UniRef100_P39592 Bacillus subtilisYwbI regulator ywbI [Bacillus subtilis] 3885 Putative HTH-typetranscriptional UniRef100_P39592 Bacillus subtilis YwbI regulator ywbI[Bacillus subtilis] 3886 3887 YwqH 3888 Holin-like protein cidA[Bacillus subtilis] UniRef100_P39591 Bacillus subtilis YwbH 3889Hypothetical protein ywbG [Bacillus UniRef100_P39590 Bacillus subtilisYwbG subtilis] 3890 HlyC domain protein [Bacillus cereus]UniRef100_Q731T5 Bacillus cereus 3891 OpuE 3892 Hypothetical UPF0064protein ywbD UniRef100_P39587 Bacillus subtilis YwbD [Bacillus subtilis]3893 Hypothetical protein ywbC [Bacillus UniRef100_P39586 Bacillussubtilis YwbC subtilis] 3894 Phosphoglycerate dehydrogenaseUniRef100_Q8GCC8 Bacillus YvcT [Bacillus coagulans] coagulans 3895Glycerate dehydrogenase UniRef100_Q8EP33 Oceanobacillus iheyensis[Oceanobacillus iheyensis] 3896 General stress protein A [BacillusUniRef100_P25148 Bacillus subtilis GspA subtilis] 3897 YdfH protein[Bacillus subtilis] UniRef100_P96685 Bacillus subtilis YdfH 3898 YdfIprotein [Bacillus subtilis] UniRef100_P96686 Bacillus subtilis YdfI 3899YdfJ protein [Bacillus subtilis] UniRef100_P96687 Bacillus subtilis YdfJ3900 Hypothetical protein ywaF [Bacillus UniRef100_P25149 Bacillussubtilis YwaF subtilis] 3901 MFS transporter, phthalate permeaseUniRef100_Q884B5 Pseudomonas YcbE family [Pseudomonas syringae] syringae3902 Hypothetical protein OB2215 UniRef100_Q8EP98 Oceanobacillus YitF[Oceanobacillus iheyensis] iheyensis 39032-dehydro-3-deoxyphosphogluconate UniRef100_Q8EP99 Oceanobacillus KdgAaldolase [Oceanobacillus iheyensis] iheyensis 39042-keto-3-deoxygluconate kinase UniRef100_Q8EPA0 Oceanobacillus KdgK[Oceanobacillus iheyensis] iheyensis 3905 Transcriptional regulatorUniRef100_Q8EPA1 Oceanobacillus KipR [Oceanobacillus iheyensis]iheyensis 3906 YwaD 3907 Hypothetical protein ywoD [BacillusUniRef100_P94574 Bacillus subtilis YwoD subtilis] 3908 3909 Hypotheticalprotein ywaC [Bacillus UniRef100_P39583 Bacillus subtilis YwaC subtilis]3910 3911 DltA 3912 Protein dltB [Bacillus subtilis] UniRef100_P39580Bacillus subtilis DltB 3913 D-alanine--poly(phosphoribitol) ligaseUniRef100_P39579 Bacillus subtilis subunit 2 [Bacillus subtilis] 3914Protein dltD precursor [Bacillus subtilis] UniRef100_P39578 Bacillussubtilis DltD 3915 Putative branched-chain-amino-acid UniRef100_P39576Bacillus subtilis YwaA aminotransferase [Bacillus subtilis] 3916Putative macrolide-efflux determinant, UniRef100_O32203 Bacillussubtilis YvqJ YvgJ [Bacillus subtilis] 3917 Probable6-phospho-beta-glucosidase UniRef100_P46320 Bacillus subtilis LicH[Bacillus subtilis] 3918 PTS system, lichenan-specific IIAUniRef100_P46319 Bacillus subtilis LicA component [Bacillus subtilis]3919 PTS system, lichenan-specific IIC UniRef100_P46317 Bacillussubtilis LicC component [Bacillus subtilis] 3920 PTS system,lichenan-specific IIB UniRef100_P46318 Bacillus subtilis component[Bacillus subtilis] 3921 Probable licABCH operon regulatorUniRef100_P46321 Bacillus subtilis LicR [Bacillus subtilis] 3922 3923Putative 3-methyladenine DNA UniRef100_P94378 Bacillus subtilis YxlJglycosylase [Bacillus subtilis] 3924 Transcriptional regulator[Rhizobium UniRef100_Q988D9 Rhizobium loti YdhC loti] 3925 Citratesynthase III [Bacillus subtilis] UniRef100_P45858 Bacillus subtilis MmgD3926 2-methylisocitrate dehydratase [Bacillus UniRef100_Q6HJ23 BacillusMmgE thuringiensis] thuringiensis 3927 Putative carboxyvinyl-UniRef100_P54528 Bacillus subtilis YqiQ carboxyphosphonatephosphorylmutase [Bacillus subtilis] 3928 Chromate transporter [BacillusUniRef100_Q6HIU6 Bacillus YwrA thuringiensis] thuringiensis 3929 BH0575protein [Bacillus halodurans] UniRef100_Q9KFB0 Bacillus halodurans 3930YwjA 3931 YesT protein [Bacillus subtilis] UniRef100_O31523 Bacillussubtilis YesT 3932 YweA 3933 YweA 3934 Drug resistance transporterBcr/CflA UniRef100_Q6HPJ5 Bacillus YdgK subfamily; possible bicyclomycinthuringiensis resistance protein [Bacillus thuringiensis] 3935 YxlGprotein [Bacillus subtilis] UniRef100_P94375 Bacillus subtilis YxlG 3936YxlF protein [Bacillus subtilis] UniRef100_P94374 Bacillus subtilis YxlF3937 YxlE protein [Bacillus subtilis] UniRef100_P94373 Bacillus subtilis3938 3939 Hypothetical protein yxlC [Bacillus UniRef100_P94371 Bacillussubtilis subtilis] 3940 RNA polymerase sigma factor sigYUniRef100_P94370 Bacillus subtilis SigY [Bacillus subtilis] 3941 YxlHprotein [Bacillus subtilis] UniRef100_P94376 Bacillus subtilis YxlH 3942Catalase [Bacillus halodurans] UniRef100_Q9KDA8 Bacillus KatX halodurans3943 Catalase [Bacillus subtilis] UniRef100_Q9AQQ9 Bacillus subtilisKatA 3944 Peroxide operon transcriptional UniRef100_Q71YY4 Listeria PerRregulator [Listeria monocytogenes] monocytogenes 3945 Ferrochelatase[Oceanobacillus UniRef100_Q8ERX9 Oceanobacillus HemH iheyensis]iheyensis 3946 Hypothetical protein yhcI [Bacillus UniRef100_P54593Bacillus subtilis YhcI subtilis] 3947 Bacitracin transport ATP-bindingUniRef100_Q81AB3 Bacillus cereus YhcH protein bcrA [Bacillus cereus]3948 MrsE protein [Bacillus sp.] UniRef100_Q9RC25 Bacillus sp. 3949 3950ABC-2 type transport system ATP- UniRef100_Q8ZZG9 Pyrobaculum YdbJbinding protein [Pyrobaculum aerophilum aerophilum] 3951 3952Transcriptional regulator [Bacillus UniRef100_Q9KFL9 Bacillus haloduranshalodurans] 3953 3954 ClyA protein [Plasmid pAD1] UniRef100_Q52055Plasmid pAD1 IspA 3955 Lantibiotic mersacidin transporterUniRef100_Q9KFM8 Bacillus YgaD system [Bacillus halodurans] halodurans3956 3957 Lantibiotic mersacidin [Bacillus UniRef100_Q9KFM5 Bacillushalodurans halodurans] 3958 Lantibiotic mersacidin modifyingUniRef100_Q9KFM4 Bacillus halodurans enzyme [Bacillus halodurans] 3959Pectate lyase Pel-34K [Bacillus sp. P- UniRef100_Q8L0S6 Bacillus sp. P-PeI 2850] 2850 3960 Hypothetical UPF0031 protein yxkO UniRef100_P94368Bacillus subtilis YxkO [Bacillus subtilis] 3961 Transport ATP-bindingprotein cydD UniRef100_P94367 Bacillus subtilis CydD [Bacillus subtilis]3962 Transport ATP-binding protein cydC UniRef100_P94366 Bacillussubtilis CydC [Bacillus subtilis] 3963 Cytochrome d ubiquinol oxidaseUniRef100_P94365 Bacillus subtilis CydB subunit II [Bacillus subtilis]3964 Cytochrome d ubiquinol oxidase UniRef100_P94364 Bacillus subtilisCydA subunit I [Bacillus subtilis] 3965 Hypothetical transport proteinywcJ UniRef100_P39608 Bacillus subtilis YwcJ [Bacillus subtilis] 3966Hypothetical protein [Bacillus cereus] UniRef100_Q735J7 Bacillus cereus3967 Probable NAD-dependent malic UniRef100_P54572 Bacillus subtilisMleA enzyme 1 [Bacillus subtilis] 3968 Malate-2H(+)/Na(+)-lactateantiporter UniRef100_P54571 Bacillus subtilis MleN [Bacillus subtilis]3969 AnsB 3970 L-asparaginase [Bacillus subtilis] UniRef100_P26900Bacillus subtilis AnsA 3971 AnsR 3972 Hypothetical protein ykgB[Bacillus UniRef100_O34499 Bacillus subtilis YkgB subtilis] 3973Hypothetical sensory transduction UniRef100_P42421 Bacillus subtilisYxdJ protein yxdJ [Bacillus subtilis] 3974 Hypothetical sensor-likehistidine UniRef100_P42422 Bacillus subtilis YxdK kinase yxdK [Bacillussubtilis] 3975 Hypothetical ABC transporter ATP- UniRef100_P42423Bacillus subtilis YxdL binding protein yxdL [Bacillus subtilis] 3976Hypothetical protein yxdM [Bacillus UniRef100_P42424 Bacillus subtilisYxdM subtilis] 3977 Hypothetical protein yxeA precursor UniRef100_P54940Bacillus subtilis YxeA [Bacillus subtilis] 3978 YxkH protein [Bacillussubtilis] UniRef100_P94361 Bacillus subtilis YxkH 3979 Transcriptionalregulator [Bacillus UniRef100_Q9KFA7 Bacillus YdeL halodurans]halodurans 3980 Hypothetical protein orfD [Lactococcus UniRef100_O86288Lactococcus YdaM lactis] lactis 3981 Citrate/malate transporter[Bacillus UniRef100_P94363 Bacillus subtilis YxkJ subtilis] 3982Glycerophosphodiester UniRef100_Q8EMK9 Oceanobacillus YhdWphosphodiesterase [Oceanobacillus iheyensis iheyensis] 3983 Hypotheticalprotein yhjA precursor UniRef100_O07555 Bacillus subtilis [Bacillussubtilis] 3984 3985 YfnI [Bacillus subtilis] UniRef100_O06487 Bacillussubtilis YfnI 3986 Hypothetical lipoprotein yfjD precursorUniRef100_O31555 Bacillus subtilis YfjD [Bacillus subtilis] 3987 YxkDprotein [Bacillus subtilis] UniRef100_P94357 Bacillus subtilis YxkD 3988Arginine repressor, argR [Bacillus UniRef100_Q81II2 Bacillus cereus AhrCcereus] 3989 Arginine deiminase [Bacillus cereus] UniRef100_Q73E87Bacillus cereus YkgA 3990 Ornithine carbamoyltransferaseUniRef100_Q73E86 Bacillus cereus ArgF [Bacillus cereus] 3991Arginine/ornithine antiporter [Bacillus UniRef100_Q73E85 Bacillus cereusYvsH cereus] 3992 Carbamate kinase [Bacillus cereus] UniRef100_Q81IH8Bacillus cereus 3993 Transcriptional regulator, Crp familyUniRef100_Q6HP25 Bacillus Fnr [Bacillus thuringiensis] thuringiensis3994 Beta-glucosidase [Oceanobacillus UniRef100_Q8ES64 OceanobacillusYdhP iheyensis] iheyensis 3995 PTS system, lichenan-specific IIcUniRef100_Q6D101 Erwinia LicC component [Erwinia carotovora] carotovora3996 Hypothetical protein [Bacillus UniRef100_Q6HC13 Bacillusthuringiensis thuringiensis] 3997 SigV 3998 Anaerobicribonucleoside-triphosphate UniRef100_O26387 Methanobacterium reductaseactivating protein thermoautotrophicum [Methanobacteriumthermoautotrophicum] 3999 Hypothetical protein yxeC [BacillusUniRef100_P54942 Bacillus subtilis subtilis] 4000 4001 4002 Hypotheticalprotein [Anabaena sp.] UniRef100_Q9AIM4 Anabaena sp. FtsH 4003 PeptidaseT [Bacillus subtilis] UniRef100_P55179 Bacillus subtilis PepT 4004Beta-fructosidase FruA [Bacillus UniRef100_Q8GM36 Bacillus SacAmegaterium] megaterium 4005 Sugar transporter FruP [BacillusUniRef100_Q8GM37 Bacillus YwbF megaterium] megaterium 4006 ABCtransporter membrane-spanning UniRef100_Q8DNN9 Streptococcus YesQpermease-sugar transport pneumoniae [Streptococcus pneumoniae] 4007 ABCtransporter membrane-spanning UniRef100_Q8DNN8 Streptococcus YurNpermease-sugar transporter pneumoniae [Streptococcus pneumoniae] 4008YesO 4009 Repressor FruR [Bacillus megaterium] UniRef100_Q8GM38 BacillusRbsR megaterium 4010 Hypothetical protein [Bacillus cereus]UniRef100_Q738I8 Bacillus cereus 4011 YjeA 4012 Hypothetical proteinyxjG [Bacillus UniRef100_P42318 Bacillus subtilis YxjG subtilis] 4013YkcB protein [Bacillus subtilis] UniRef100_O34575 Bacillus subtilis YkcB4014 Putative glycosyl transferase ykcC UniRef100_O34319 Bacillussubtilis YkcC [Bacillus subtilis] 4015 GalE 4016 4017 4018 4019Hypothetical 10.1 kDa protein [Bacillus UniRef100_O50571 Bacilluspseudofirmus pseudofirmus] 4020 4021 Hypothetical transport protein yxjAUniRef100_P42312 Bacillus subtilis YxjA [Bacillus subtilis] 4022Catalase 2 [Bacillus subtilis] UniRef100_P42234 Bacillus subtilis KatE4023 Catalase 2 [Bacillus subtilis] UniRef100_P42234 Bacillus subtilisKatE 4024 DNA gyrase inhibitory protein, GyrI UniRef100_Q97DI7Clostridium YosT [Clostridium acetobutylicum] acetobutylicum 4025Probable glycosyl hydrolase [Erwinia UniRef100_Q6D774 Erwinia YckEcarotovora] carotovora 4026 PTS system, beta-glucoside-specificUniRef100_Q9KG19 Bacillus BglP enzyme II, ABC component [Bacillushalodurans halodurans] 4027 Transcription antiterminator licTUniRef100_P39805 Bacillus subtilis LicT [Bacillus subtilis] 4028Hypothetical conserved protein UniRef100_Q8ES40 Oceanobacillus YkoC[Oceanobacillus iheyensis] iheyensis 4029 Cation ABC transporterATP-binding UniRef100_Q8ES39 Oceanobacillus YkoD protein [Oceanobacillusiheyensis] iheyensis 4030 Hypothetical conserved proteinUniRef100_Q8ES38 Oceanobacillus YkoE [Oceanobacillus iheyensis]iheyensis 4031 Transcriptional activator of extracellularUniRef100_Q8ESZ0 Oceanobacillus TenA enzyme genes [Oceanobacillusiheyensis iheyensis] 4032 Isocitrate lyase [Bacillus cereus]UniRef100_Q73C38 Bacillus cereus YqiQ 4033 Malate synthase A [Bacilluscereus] UniRef100_Q73C39 Bacillus cereus 4034 4035 Hypothetical proteinyycA [Bacillus UniRef100_P37483 Bacillus subtilis YycA subtilis] 4036Bacitracin export permease protein UniRef100_O34741 Bacillus subtilisYtsD bceB [Bacillus subtilis] 4037 Bacitracin export ATP-binding proteinUniRef100_O34697 Bacillus subtilis YtsC bceA [Bacillus subtilis] 4038Sensor protein bceS [Bacillus subtilis] UniRef100_O35044 Bacillussubtilis YtsB 4039 Sensory transduction protein bceR UniRef100_O34951Bacillus subtilis YtsA [Bacillus subtilis] 4040 Beta-glucosidase[Bacillus subtilis] UniRef100_P40740 Bacillus subtilis BglH 4041 PTSsystem, beta-glucoside-specific UniRef100_P40739 Bacillus subtilis BglPIIABC component [Bacillus subtilis] 4042 Hypothetical protein yxeG[Bacillus UniRef100_P54946 Bacillus subtilis YxeG subtilis] 4043Hypothetical protein yxeI [Bacillus UniRef100_P54948 Bacillus subtilisYxeI subtilis] 4044 4045 4046 Hypothetical protein yxiA precursorUniRef100_P42293 Bacillus subtilis YxiA [Bacillus subtilis] 4047ATP-dependent RNA helicase dbpA UniRef100_P42305 Bacillus subtilis DeaD[Bacillus subtilis] 4048 Hypothetical protein OB2811 UniRef100_Q8EMN1Oceanobacillus iheyensis [Oceanobacillus iheyensis] 4049 Hypotheticalprotein OB2810 UniRef100_Q8EMN2 Oceanobacillus iheyensis [Oceanobacillusiheyensis] 4050 YxiD 4051 4052 BH4015 protein [Bacillus halodurans]UniRef100_Q9K5S3 Bacillus YxiB halodurans 4053 Pyrimidine-nucleosidephosphorylase UniRef100_P39142 Bacillus subtilis Pdp [Bacillus subtilis]4054 Pyrimidine nucleoside transport protein UniRef100_P39141 Bacillussubtilis NupC [Bacillus subtilis] 4055 Deoxyribose-phosphate aldolaseUniRef100_P39121 Bacillus subtilis Dra [Bacillus subtilis] 4056Deoxyribonucleoside regulator [Bacillus UniRef100_P39140 Bacillussubtilis DeoR subtilis] 4057 Hypothetical protein [Bacillus anthracis]UniRef100_Q81X49 Bacillus anthracis 4058 YdaJ protein [Bacillussubtilis] UniRef100_P96584 Bacillus subtilis YdaJ 4059 YdaK protein[Bacillus subtilis] UniRef100_P96585 Bacillus subtilis YdaK 4060 YdaLprotein [Bacillus subtilis] UniRef100_O31487 Bacillus subtilis YdaL 4061YdaM protein [Bacillus subtilis] UniRef100_P96587 Bacillus subtilis YdaM4062 YdaN protein [Bacillus subtilis] UniRef100_O31488 Bacillus subtilisYdaN 4063 Valine-pyruvate aminotransferase UniRef100_Q8YTB2 Anabaena sp.AlaT [Anabaena sp.] 4064 Hypothetical protein [Leifsonia xyli]UniRef100_Q6AEW0 Leifsonia xyli 4065 Hypothetical protein [Leifsoniaxyli] UniRef100_Q6AEW1 Leifsonia xyli YwfB 4066 Similarities withputative carboxylase UniRef100_Q7NA29 Photorhabdus YwfE [Photorhabdusluminescens] luminescens 4067 Transporter, Drug/Metabolite ExporterUniRef100_Q63AK7 Bacillus cereus YoaV family [Bacillus cereus ZK] ZK4068 Probable fructose-bisphosphate UniRef100_P42420 Bacillus subtilisFbaB aldolase 2 [Bacillus subtilis] 4069 IolI protein [Bacillussubtilis] UniRef100_P42419 Bacillus subtilis IolI 4070 IolH protein[Bacillus subtilis] UniRef100_P42418 Bacillus subtilis IolH 4071Myo-inositol dehydrogenase [Bacillus UniRef100_Q6B6R7 Bacillus subtilisIdh subtilis] 4072 Myo-inositol transport protein [BacillusUniRef100_P42417 Bacillus subtilis IolF subtilis] 4073 IolE protein[Bacillus subtilis] UniRef100_P42416 Bacillus subtilis IolE 4074Probable malonic semialdehyde UniRef100_P42415 Bacillus subtilis IolDoxidative decarboxylase [Bacillus subtilis] 4075 Protein iolC [Bacillussubtilis] UniRef100_P42414 Bacillus subtilis IolC 4076 IolB protein[Bacillus subtilis] UniRef100_P42413 Bacillus subtilis IolB 4077Probable methylmalonate- UniRef100_P42412 acylating MmsA semialdehydedehydrogenase [acylating] [Bacillus subtilis] 4078 DNA-binding proteiniolR [Bacillus UniRef100_P46337 Bacillus subtilis IolR subtilis] 4079IolS protein [Bacillus subtilis] UniRef100_P46336 Bacillus subtilis IolS4080 UPI00003CB380 UniRef100 entry UniRef100_UPI00003CB380 GlpQ 4081Putative glycerol-3-phosphate UniRef100_Q6GJY0 Staphylococcus GlpTtransporter [Staphylococcus aureus] aureus 4082 Chaperone protein htpG[Bacillus UniRef100_P46208 Bacillus subtilis HtpG subtilis] 4083 YfmM4084 Hypothetical protein yxeH [Bacillus UniRef100_P54947 Bacillussubtilis YxeH subtilis] 4085 Glycerol dehydrogenase [ClostridiumUniRef100_Q97IL4 Clostridium AraM acetobutylicum] acetobutylicum 4086Phosphoserine phosphatase family UniRef100_Q97IL5 Clostridium YkrXprotein [Clostridium acetobutylicum] acetobutylicum 4087 BH0833 protein[Bacillus halodurans] UniRef100_Q9KEL9 Bacillus halodurans 4088 YxeB4089 4090 BH3956 protein [Bacillus halodurans] UniRef100_Q9K5Y0 BacillusYraH halodurans 4091 4092 Ferrous iron transport protein BUniRef100_Q632N2 Bacillus cereus ZK [Bacillus cereus ZK] 4093 Lmo2104protein [Listeria UniRef100_Q929R6 Listeria monocytogenes monocytogenes]4094 YfiQ protein [Bacillus subtilis] UniRef100_O31559 Bacillus subtilisYfiQ 4095 ABC transporter permease protein UniRef100_Q81EI1 Bacilluscereus YxdM [Bacillus cereus] 4096 ABC transporter ATP-binding proteinUniRef100_Q81D39 Bacillus cereus YxdL [Bacillus cereus] 4097 Sensorhistidine kinase [Bacillus cereus UniRef100_Q63AY4 Bacillus cereus YxdKZK] ZK 4098 UPI00003CBD9F UniRef100 entry UniRef100_UPI00003CBD9F YxdJ4099 Hypothetical protein [Bacillus cereus] UniRef100_Q72X82 Bacilluscereus 4100 YoaH [Bacillus subtilis] UniRef100_O34576 Bacillus subtilisYoaH 4101 Amino acid transporter [Bacillus UniRef100_Q9K609 BacillusYflA halodurans] halodurans 4102 Alanine dehydrogenase [BacillusUniRef100_Q9KAF8 Bacillus Ald halodurans] halodurans 4103 Hypotheticalfamily 53 glycosyl UniRef100_O07013 Bacillus subtilis YvfO hydrolaseyvfO precursor [Bacillus subtilis] 4104 Hypothetical protein yvfN[Bacillus UniRef100_O07012 Bacillus subtilis LacA subtilis] 4105Hypothetical protein yvfM [Bacillus UniRef100_O07011 Bacillus subtilisYvfM subtilis] 4106 Maltose/maltodextrin transport systemUniRef100_Q9KBA8 Bacillus YvfL [Bacillus halodurans] halodurans 4107Hypothetical protein yvfK [Bacillus UniRef100_O07009 Bacillus subtilisYvfK subtilis] 4108 LacR 4109 Galactokinase [Streptococcus gordonii]UniRef100_Q840N8 Streptococcus GalK gordonii 4110 UDP-glucose4-epimerase [Listeria UniRef100_Q928B6 Listeria innocua GalE innocua]4111 UPI00003CB9B6 UniRef100 entry UniRef100_UPI00003CB9B6 GalT 4112Transcriptional repressor of the xylose UniRef100_Q9KDW7 Bacillus XylRoperon [Bacillus halodurans] halodurans 4113 GntR 4114 GntK 4115 GntP4116 GntZ 4117 Alcohol dehydrogenase [Bacillus UniRef100_Q818A4 Bacilluscereus GbsB cereus] 4118 Alkyl hydroperoxide reductase subunitUniRef100_P80239 Bacillus subtilis AhpC C [Bacillus subtilis] 4119 NADHdehydrogenase [Bacillus subtilis] UniRef100_P42974 Bacillus subtilisAhpF 4120 4121 4122 4123 YvfR 4124 Hypothetical protein yvfS [BacillusUniRef100_O07017 Bacillus subtilis YvfS subtilis] 4125 YvfT 4126Hypothetical protein yvfU [Bacillus UniRef100_O07019 Bacillus subtilisYvfU subtilis] 4127 4128 4129 YtrE 4130 4131 4132 4133 4134 Hypotheticalprotein [Desulfotalea UniRef100_Q6ARQ4 Desulfotalea psychrophilapsychrophila] 4135 Hypothetical protein [Desulfotalea UniRef100_Q6ARQ5Desulfotalea psychrophila psychrophila] 4136 4137 4138 Putative zincmetallopeptidase UniRef100_Q894N2 Clostridium tetani [Clostridiumtetani] 4139 Type I restriction-modification system UniRef100_Q6MH63Bdellovibrio bacteriovorus restriction subunit [Bdellovibriobacteriovorus] 4140 Lin0523 protein [Listeria innocua] UniRef100_Q92ED7Listeria innocua 4141 4142 Hypothetical protein CAC1662 UniRef100_Q97IH9Clostridium acetobutylicum [Clostridium acetobutylicum] 4143 Type Irestriction-modification system, UniRef100_Q72BC7 Desulfovibrio vulgarisM subunit [Desulfovibrio vulgaris] 4144 4145 4146 4147 UPI00002C7192UniRef100 entry UniRef100_UPI00002C7192 4148 Cassette chromosomerecombinase B UniRef100_Q8RPD2 Staphylococcus SpoIVCA [Staphylococcusaureus] aureus 4149 Hypothetical UPF0247 protein yyda UniRef100_Q45601Bacillus subtilis YydA [Bacillus subtilis] 4150 4151 4152 YycN protein[Bacillus subtilis] UniRef100_O32293 Bacillus subtilis YycN 4153 4154UPI00003CA59D UniRef100 entry UniRef100_UPI00003CA59D 4155 Hypotheticalserine protease yyxA UniRef100_P39668 Bacillus subtilis YyxA [Bacillussubtilis] 4156 YycJ protein [Bacillus subtilis] UniRef100_Q45611Bacillus subtilis YycJ 4157 YycI protein [Bacillus subtilis]UniRef100_Q45612 Bacillus subtilis YycI 4158 YycH protein [Bacillussubtilis] UniRef100_Q45613 Bacillus subtilis YycH 4159 Sensor proteinyycG [Bacillus subtilis] UniRef100_Q45614 Bacillus subtilis YycG 4160Transcriptional regulatory protein yycF UniRef100_P37478 Bacillussubtilis YycF [Bacillus subtilis] 4161 Phosphohydrolase [Bacilluscereus] UniRef100_Q81103 Bacillus cereus 4162 Adenylosuccinatesynthetase [Bacillus UniRef100_P29726 Bacillus subtilis PurA subtilis]4163 4164 Replicative DNA helicase [Bacillus UniRef100_P37469 Bacillussubtilis DnaC subtilis] 4165 Hypothetical protein yycD [BacillusUniRef100_P37480 Bacillus subtilis subtilis] 4166 Hypothetical proteinyyzB [Bacillus UniRef100_O32296 Bacillus subtilis subtilis] 4167Hypothetical transport protein yycB UniRef100_P37482 Bacillus subtilisYycB [Bacillus subtilis] 4168 N-acetylglucosamine-6-phosphateUniRef100_Q9KFQ7 Bacillus NagA deacetylase [Bacillus halodurans]halodurans 4169 NagB 4170 Transcriptional regulator [BacillusUniRef100_Q9KFQ9 Bacillus YvoA halodurans] halodurans 4171 Hypotheticalprotein VP0543 [Vibrio UniRef100_Q87S81 Vibrio YbbI parahaemolyticus]parahaemolyticus 4172 PTS system, n-acetylglucosamine- UniRef100_Q9KF24Bacillus NagP specific enzyme II, ABC component halodurans [Bacillushalodurans] 4173 50S ribosomal protein L9 [Bacillus UniRef100_P37437Bacillus subtilis RplI subtilis] 4174 Hypothetical protein yybT[Bacillus UniRef100_P37484 Bacillus subtilis YybT subtilis] 4175Hypothetical protein yybS [Bacillus UniRef100_P37485 Bacillus subtilisYybS subtilis] 4176 Spore coat protein F precursor [BacillusUniRef100_P23261 Bacillus subtilis CotF subtilis] 4177 Universal stressprotein, Usp family UniRef100_Q6HIV0 Bacillus YxiE [Bacillusthuringiensis] thuringiensis 4178 Sulfate permease [BacillusUniRef100_Q6HIU9 Bacillus YbaR thuringiensis] thuringiensis 4179Hypothetical transport protein yybO UniRef100_P37489 Bacillus subtilisYybO [Bacillus subtilis] 4180 30S ribosomal protein S18 [BacillusUniRef100_P21475 Bacillus subtilis subtilis] 4181 Single-strand bindingprotein [Bacillus UniRef100_P37455 Bacillus subtilis Ssb subtilis] 418230S ribosomal protein S6 [Bacillus UniRef100_P21468 Bacillus subtilissubtilis] 4183 GTP-dependent nucleic acid-binding UniRef100_P37518Bacillus subtilis YyaF protein engD [Bacillus subtilis] 4184 Lin2921protein [Listeria innocua] UniRef100_Q926W9 Listeria innocua 4185Hypothetical protein yyaD [Bacillus UniRef100_P37520 Bacillus subtilisYyaD subtilis] 4186 Hypothetical protein yyaC [Bacillus UniRef100_P37521Bacillus subtilis YyaC subtilis] 4187 Stage 0 sporulation protein J[Bacillus UniRef100_P26497 Bacillus subtilis Spo0J subtilis] 4188 Soj4189 Hypothetical protein [Burkholderia UniRef100_Q63L10 BurkholderiaYdfG pseudomallei K96243] pseudomallei K96243 4190 RNA polymerase sigmafactor, ECF UniRef100_Q63BC0 Bacillus cereus SigM subfamily [Bacilluscereus ZK] ZK 4191 YyaA protein [Bacillus subtilis] UniRef100_P37524Bacillus subtilis YyaA 4192 Methyltransferase gidB [BacillusUniRef100_P25813 Bacillus subtilis GidB subtilis] 4193 Glucose inhibiteddivision protein A UniRef100_P25812 Bacillus subtilis GidA [Bacillussubtilis] 4194 Probable tRNA modification GTPase UniRef100_P25811Bacillus subtilis ThdF trmE [Bacillus subtilis] 4195 Jag protein[Bacillus subtilis] UniRef100_Q01620 Bacillus subtilis Jag 4196 SpoIIIJ4197 Ribonuclease P protein component UniRef100_P25814 Bacillus subtilisRnpA [Bacillus subtilis]

TABLE 2 Features of the Bacillus licheniformis genome and comparisonwith genomes of other Bacillus species. Feature B. licheniformis B.subtilis ^(a) B. halodurans ^(b) Oceanobacillus iheyensis ^(c) B.anthracis ^(d) B. cereus ^(e) Chromosome Size (bp) 4,222,336 4,214,6304,202,352 3,630,528 5,227,293 5,426,909 G + C content (mol %) 46.2 43.543.7 35.7 35.4 35.4 Protein coding sequences 4197 4106 4066 3496 55085366 Average length (bp) 873 896 879 883 800 835 Percent of codingregion 87 87 85 85 84 84 Ribosomal RNA operons 7 10 8 7 11 13 Number oftRNAs 81 86 78 69 95 108 Phage-associated genes 71 268 42 27 62 124Transposase genes of 10 0 93 14 18 10 IS-elements ^(a)Kunst, F.,Ogasawara, N, Mozser, I., Albertini, A. M., Alloni, G., Azebedo, V.,Bertero, M. G., Bessieres, P., Bolotin, A., and Borchert, S. et al.(1997) Nature 390, 249-256. ^(b)Takami, H., Nakasone, K., Takaki, Y.,Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y.,Ogasawara, N. et al. (2000) Nucleic Acids Res. 28, 4317-4331.^(c)Takami, H., Takaki, Y., and Uchiyama, I. (2002) Nucleic Acids Res.30, 3927-3935. ^(d)Read, T. D., Peterson, S. N., Tourasse, N., Baillie,L. W., Paulsen, I. T., Nelson, K. E., Tettelin, H., Fouts, D. E., Eisen,J. A., and Gill, S. R. et al. (2003) Nature 423, 81-86. ^(e)Ivanova, N,Sorokin, A., Anderson, I, Galleron, N., Candelon, B., Kapatral, V.,Bhattacharyya, A., Reznik, G., Mikhailova, N., and Lapidus, A. et al.(2003) Nature 423, 87-91.

TABLE 3 Extracellular proteins predicted in the Bacillus licheniformisgenome Signal SEQ ID Gene Peptide B. subtilis NO. Name Location PutativeProduct Putative Function Gene 11 dacA [1-32] D-alanyl-D-alanine“Molecular Function: serine DacA carboxypeptidase (penicillin-carboxypeptidase activity binding protein 5) (GO:0004185), BiologicalProcess: proteolysis and peptidolysis (GO:006508)” 45 yabE [1-32]conserved hypothetical containing domain DUF348 YabE YabE 67 divlC[1-66] cell-division initiation protein “required for both vegetativeand DivlC sporulation septum formation, Biological Process: cell cycle(GO:0007049)” 157 BL01016 [1-28] N-acetylmuramoyl-L-alanine “cell wallhydrolase, Molecular CwlD amidase Function: N-acetylmuramoyl-L- alanineamidase activity (GO:0008745), Biological Process: peptidoglycancatabolism (GO:0009253)” 159 gerD [1-27] GerD “germination response toL- GerD alanine and to the combination of glucose, fructose, L-asparagine, and KCl (early stage),” 161 ybaN [1-42] “Polysaccharide“Biological Process: YbaN deacetylase, Carbohydrate carbohydratemetabolism Esterase Family 4” (GO:0005975), Molecular Function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds(GO:0016810)” 162 pbpX [1-43] penicillin-binding protein PbpX 167 ybbC[1-23] conserved hypothetical protein YbbC YbbC 168 ybbD [1-27]“Glycoside hydrolase, family 3” “Molecular Function: hydrolase YbbDactivity, hydrolyzing O-glycosyl compounds (GO:0004553), BiologicalProcess: carbohydrate metabolism (GO:0005975)” 169 ybbE [1-22] putativebeta-lactamase YbbE YbbE 227 [1-34] putative ribose ABC transporter“ribose transport,” RbsB (ribose-binding protein) 228 yomI [1-32]putative lytic transglycosylase YomI YomI 247 penP [1-35] beta-lactamaseprecursor “Molecular Function: aspartic- PenP type endopeptidaseactivity (GO:0004190), Biological Process: proteolysis and peptidolysis(GO:0006508)” 249 tatAD [1-35] component of the twin-arginine“Biological Process: protein transport pre-protein translocation(GO:0015031), Cellular Component: integral pathway to membrane(GO:0016021)” 267 [1-26] “putative Proteinase inhibititor I4, MolecularFunction: serine-type serpin” endopeptidase inhibitor activity(GO:0004867) 268 BL01663 [1-22] Pectin lyase-like YbdN 287 BL01793[1-31] putative lipoprotein YcdA 303 yvbX [1-33] “conserved protein,Glycoside “Molecular Function: catalytic YvbX Hydrolase Family 18, YvbX”activity (GO:0003824), Biological Process: carbohydrate metabolism(GO:0005975)” 304 [1-27] “Chitinase precursor, Glycoside “MolecularFunction: catalytic activity Hydrolase Family 18” (GO:0003824),Biological Process: carbohydrate metabolism (GO:0005975)” 312 yvcE[1-31] “putative peptidoglycan hydrolase, DL-endopeptidase II family”YvcE 348 yckB [1-34] putative extracellular solute- “Molecular Function:transporter YckB binding protein YckB activity (GO:0005215), BiologicalProcess: transport (GO:0006810), Cellular Component: periplasmic space(sensu Gram-negative Bacteria) (GO:0030288)” 353 nucA [1-37] nucleaseNucA NucA 359 BL01722 [1-34] putative extracellular solute- “MolecularFunction: transporter YvfK binding protein activity (GO:0005215),Biological Process: transport (GO:0006810)” 373 [1-25] hypotheticalprotein 382 BL01829 [1-25] “putative extracellular solute- “MolecularFunction: transporter YckK binding protein, family 3” activity(GO:0005215), Biological Process: transport (GO:0006810), CellularComponent: periplasmic space (sensu Gram-negative Bacteria)(GO:0030288)” 403 BL01746 [1-28] ribose ABC transporter (ribose- “ribosetransport,” RbsB binding protein) 409 phy [1-30] phytase “hydrolysis ofphytate into Phy inorganic phosphate and myo- inositol,” 426 yclQ [1-25]Periplasmic binding protein “Molecular Function: iron ion YclQtransporter activity (GO:0005381), Biological Process: high affinityiron ion transport (GO:0006827)” 438 [1-26] hypothetical protein 472BL02821 [1-34] Cell envelope-related transcriptional attenuator YvhJ 483[1-42] hypothetical protein 513 ydcC [1-28] conserved membrane proteinYdcC YdcC 552 yrhM [1-68] YrhM YrhM 558 ywpE [1-26] conserved proteinYwpE “Biological Process: biosynthesis (GO:0009058), Molecular Function:transferase activity (GO:0016740)” 576 [1-34] putative transporter“Molecular Function: transporter activity (GO:0005215), BiologicalProcess: transport (GO:0006810), Cellular Component: membrane(GO:0016020)” 605 yjeAA [1-37] conserved hypothetical protein YjeA 607amyL [1-30] “alpha amylase, Glycoside “Molecular Function: alpha- TreAHydrolase Family 13” amylase activity (GO:0004556), Biological Process:carbohydrate metabolism (GO:0005975)” 610 yvdG [1-28] putativemaltose/maltodextrin “Molecular Function: transporter YvdG transportsystem substrate- activity (GO:0005215), Biological binding proteinProcess: transport (GO:0006810)” 619 [1-37] hypothetical protein 620[1-28] hypothetical protein 621 ydjM [1-41] YdjM YdjM 622 ydjN [1-21]YdjN YdjN 660 yerB [1-28] conserved protein YerB YerB 665 yerH [1-26]YerH YerH 683 BL05063 [1-35] mannan endo-1,4-beta- Degradation of mannanpolysaccharides mannosidase 733 [1-29] putative carboxypeptidase“Molecular Function: carboxypeptidase A activity (GO:0004182),Biological Process: proteolysis and peptidolysis (GO:0006508)” 773 yfkD[1-26] conserved protein YfkD YfkD 776 BL03088 [1-24] “Polysaccharidedeacetylase, “Biological Process: YfjS Carbohydrate Esterase Familycarbohydrate metabolism 4” (GO:0005975), Molecular Function: hydrolaseactivity, acting on carbon-nitrogen (but not peptide) bonds(GO:0016810)” 784 yfjL [1-33] putative transporter YfjL “MolecularFunction: transporter YfjL activity (GO:0005215), Biological Process:transport (GO:0006810), Cellular Component: membrane (GO:0016020)” 787BL03096 [1-26] hypothetical protein 813 BL03024 [1-26] “putativeextracellular solute- “Molecular Function: transporter YtcQ bindingprotein, family 1” activity (GO:0005215), Biological Process: transport(GO:0006810)” 841 appAC [1-30] oligopeptide ABC transporter “requiredfor initiation of OppA (binding protein) sporulation, competencedevelopment, and oligopeptide transport, Molecular Function: transporteractivity (GO:0005215), Biological Process: transport (GO:0006810)” 866ssuA [1-24] aliphatic sulfonate ABC transporter (binding lipoprotein)SsuA 876 [1-23] hypothetical protein 886 [1-23] hypothetical protein 892[1-28] hypothetical protein YhcC 900 BL03178 [1-27] putative lipoproteinYhcJ 902 BL03161 [1-31] hypothetical protein YhcM 904 BL03176 [1-29]hypothetical protein YhcN 905 BL03175 [1-30] hypothetical protein YhcP934 lytE [1-26] cell wall hydrolase phosphatase- “cell wall lyticactivity, Biological LytE associated protein Process: cell wallcatabolism (GO:0016998), Biological Process: cell wall catabolism(GO:0016998)” 937 [1-26] putative glucose dehydrogenase 965 BL02841[1-41] “Polysaccharide deacetylase, “Biological Process: YheNCarbohydrate Esterase Family carbohydrate metabolism 4” (GO:0005975),Molecular Function: hydrolase activity, acting on carbon-nitrogen (butnot peptide) bonds (GO:0016810)” 996 prsA [1-30] molecular chaperonePrsA “essential for the stability of PrsA secreted proteins at stagesfollowing translocation across the membrane, Molecular Function:isomerase activity (GO:0016853)” 1001 BL02907 [1-31] hypotheticalprotein YhaH 1032 yhfQ [1-32] putative transferase “Biological Process:metabolism YhfQ (GO:0008152), Molecular Function: transferase activity(GO:0016740)” 1044 epr [1-27] extracellular serine protease “MolecularFunction: subtilase Epr activity (GO:0004289), Biological Process:proteolysis and peptidolysis (GO:0006508), Molecular Function: subtilaseactivity (GO:0004289), Biological Process: proteolysis and peptidolysis(GO:0006508)” 1061 [1-24] hypothetical protein 1063 msmE [1-29] multiplesugar-binding protein “Molecular Function: transporter MsmE MsmEactivity (GO:0005215), Biological Process: transport (GO:0006810)” 1073BL01323 [1-28] conserved hypothetical protein LytB 1077 yvgL [1-29]molybdate transport system “Molecular Function: molybdate- YvgLsubstrate-binding protein transporting ATPase activity (GO:0015412),Biological Process: molybdate ion transport (GO:0015689)” 1113 BL01309[1-24] Short-chain “Biological Process: metabolism YcdFdehydrogenase/reductase SDR (GO:0008152), Molecular Function:oxidoreductase activity (GO:0016491)” 1150 appA [1-32] oligopeptide ABCtransporter “oligopeptide transport, Molecular AppA(oligopeptide-binding protein) Function: transporter activity(GO:0005215), Biological Process: transport (GO:0006810)” 1156 oppA[1-29] oligopeptide ABC transporter “required for initiation of OppA(binding protein) sporulation, competence development, and oligopeptidetransport, Molecular Function: transporter activity (GO:0005215),Biological Process: transport (GO:0006810)” 1165 BL03332 [1-31]conserved hypothetical protein YflP 1216 abnAA [1-26] GlycosideHydrolase Family 43 “degradation of plant cell wall AbnA polysaccharide,Molecular Function: hydrolase activity, hydrolyzing O-glycosyl compounds(GO:0004553), Biological Process: carbohydrate metabolism (GO:0005975)”1219 [1-30] hypothetical protein 1226 BL01957 [1-23] conservedhypothetical protein YoeB 1227 BL01953 [1-26] Peptidoglycan-bindingprotein “Biological Process: cell wall YocH catabolism (GO:0016998),Biological Process: cell wall catabolism (GO:0016998)” 1283 yesO [1-28]putative transport system “Molecular Function: transporter YesOsubstrate-binding protein YesO activity (GO:0005215), BiologicalProcess: transport (GO:0006810)” 1291 yesW [1-33] putativepolysaccharide lyase family 11 protein YesW 1299 lplA [1-32] lipoprotein“Molecular Function: transporter LplA activity (GO:0005215), BiologicalProcess: transport (GO:0006810)” 1311 dppE [1-27] dipeptide ABCtransporter “Molecular Function: transporter DppE (dipeptide-bindingprotein) activity (GO:0005215), Biological Process: transport(GO:0006810)” 1319 pelI [1-25] “pectate lyase family 1, PelI” Pel 1332[1-30] hypothetical protein 1341 [1-27] hypothetical protein 1345 [1-27]hypothetical protein YonS 1368 [1-28] hypothetical protein 1415 pbpC[1-30] penicillin-binding protein 3 Molecular Function: penicillin PbpCbinding (GO:0008658) 1448 [1-28] hypothetical protein 1475 BL05139[1-27] glycerophosphoryl diester “hydrolysis of deacylated GlpQphosphodiesterase phospholipids, Biological Process: glycerol metabolism(GO:0006071), Molecular Function: glycerophosphodiesterphosphodiesterase activity (GO:0008889)” 1479 [1-27] hypotheticalprotein 1493 [1-27] hypothetical protein 1496 BL03556 [1-25] putativeCell wall hydrolase YkvT 1498 ykvV [1-28] hypothetical protein Containsthioredoxin domain 2 YkvV 1501 [1-25] hypothetical protein 1512 ykwC[1-24] Hypothetical oxidoreductase YkwC 1513 BL03646 [1-27] AllergenV5/Tpx-1 related Cellular Component: YkwD extracellular (GO:0005576)1527 [1-28] hypothetical protein 1553 BL01601 [1-32] “putative secretionprotein, “Molecular Function: protein YknX protein transporter”transporter activity (GO:0008565), Biological Process: protein secretion(GO:0009306), Cellular Component: membrane (GO:0016020)” 1565 [1-40]hypothetical protein 1576 ykyA [1-29] Hypothetical protein ykyA YkyA1601 BL02997 [1-27] hypothetical protein YlaJ 1617 ylbC [1-34] conservedhypothetical protein Cellular Component: YlbC YlbC extracellular(GO:0005576) 1625 ylbL [1-29] YlbL Molecular Function: protein YlbLbinding (GO:0005515) 1635 ftsL [1-59] cell-division protein “septumformation (early stage),” FtsL 1636 pbpB [1-42] penicillin-bindingprotein 2B “formation of the cell-division PbpB septum (late stage),”1644 divlB [1-57] cell-division initiation protein “probably involved instabilizing DivlB or promoting the assembly of the division complex(septum formation),” 1645 BL02246 [1-29] conserved hypothetical proteinYlxW 1646 BL02248 [1-30] hypothetical protein YlxX 1650 bprA [1-28]bacillopeptidase F “Molecular Function: subtilase Bpr activity(GO:0004289), Biological Process: proteolysis and peptidolysis(GO:0006508)” 1651 bprB [1-31] bacillopeptidase F “Molecular Function:subtilase Bpr activity (GO:0004289), Biological Process: proteolysis andpeptidolysis (GO:0006508)” 1752 fliL [1-32] flagellar protein “requiredfor flagellar FliL formation, Biological Process: ciliary/flagellarmotility (GO:0001539), Biological Process: chemotaxis (GO:0006935),Cellular Component: flagellar basal body (sensu Bacteria) (GO:0009425)”1781 celA [1-34] Glycoside Hydrolase Family 9 “Molecular Function:hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553),Biological Process: carbohydrate metabolism (GO:0005975)” 1783 celC[1-34] Glycoside Hydrolase Family 5 “Molecular Function: hydrolaseactivity, hydrolyzing O-glycosyl compounds (GO:0004553), BiologicalProcess: carbohydrate metabolism (GO:0005975)” 1784 celD [1-32]Glycoside Hydrolase family 5 “Molecular Function: hydrolase activity,hydrolyzing O-glycosyl compounds (GO:0004553), Biological Process:carbohydrate metabolism (GO:0005975)” 1820 BL03655 [1-31] conservedhypothetical protein YmdA 1839 [1-29] hypothetical protein 1904 yoaO[1-38] YoaO YoaO 1914 yoaW [1-26] YoaW YoaW 1926 [1-38] phage-likeprotein 1929 nucB [1-34] nuclease NucB 1931 BL05188 [1-29]Peptidoglycan-binding protein Biological Process: cell wall YneAcatabolism (GO:0016998) 1946 yneN [1-29] putative thiol: disulfide“Molecular Function: electron YneN interchange protein YneN transporteractivity (GO:0005489), Biological Process: electron transport(GO:0006118)” 1983 bglC [1-50] “endo-1,4-beta-glucanase, “MolecularFunction: hydrolase BglC Glycoside hydrolase Family 5” activity,hydrolyzing O-glycosyl compounds (GO:0004553), Biological Process:carbohydrate metabolism (GO:0005975),” 1990 ywoF [1-22] “PolysaccharideLyase Family 9, YwoF” YwoF 1995 BL00297 [1-27] conserved hypotheticalprotein YddR 2011 yjmF [1-31] Short-chain “Biological Process:metabolism YjmF dehydrogenase/reductase SDR (GO:0008152), MolecularFunction: oxidoreductase activity (GO:0016491)” 2016 dctB [1-29]possible C4-dicarboxylate “, Biological Process: transport DctB bindingprotein (GO:0006810), Cellular Component: periplasmic space (sensuGram-negative Bacteria) (GO:0030288)” 2028 dacC [1-29]penicillin-binding protein (D- “Molecular Function: serine DacCalanyl-D-alanine carboxypeptidase activity carboxypeptidase)(GO:0004185), Biological Process: proteolysis and peptidolysis(GO:0006508)” 2060 [1-37] hypothetical protein 2072 BL00899 [1-28]“Spore germination B3 GerAC like, C-terminal” YndF 2105 [1-33] conservedhypothetical protein 2108 BL01303 [1-26] Peptidoglycan-binding protein“Biological Process: cell wall YocH catabolism (GO:0016998), BiologicalProcess: cell wall catabolism (GO:0016998)” 2123 [1-36] hypotheticalprotein 2132 BL01404 [1-24] hypothetical protein YoqH 2152 yvgO [1-25]conserved protein YvgO YvgO 2160 lytF [1-27] gamma-D-glutamate-meso-“cell wall lytic activity, Biological LytF diaminopimelate muropeptidaseProcess: cell wall catabolism (major autolysin) (CWBP49′) (GO:0016998)”2167 yoaJ [1-26] YoaJ YoaJ 2177 ctpA [1-37] carboxy-terminal processing“, Biological Process: proteolysis CtpA protease and peptidolysis(GO:0006508), Molecular Function: serine-type peptidase activity(GO:0008236)” 2180 yodJ [1-31] putative carboxypeptidase YodJ 2207 ypmS[1-34] conserved protein YpmS YpmS 2208 ypmR [1-24] conserved proteinYpmR Molecular Function: catalytic YpmR activity (GO:0003824) 2209 ypmQ[1-25] conserved hypothetical YpmQ Biological Process: electron YpmQtransport (GO:0006118) 2218 ypjP [1-30] conserved hypothetical proteinYpjP YpjP 2266 ponA [1-63] “penicillin-binding “involved in divisionseptum PonA proteins, Glycosyl Transferase formation,” Family 51” 2271aspB [1-23] aspartate aminotransferase “Molecular Function: AspBtransaminase activity (GO:0008483), Biological Process: biosynthesis(GO:0009058)” 2272 ypmB [1-25] conserved protein YpmB YpmB 2315 BL02789[1-29] hypothetical protein YphF 2328 sleB [1-34] spore cortex-lyticenzyme SleB 2330 ansZA [1-18] putative “Biological Process: amino acidYccC Asparaginase/glutaminase metabolism (GO:0006520), BiologicalProcess: amino acid metabolism (GO:0006520)” 2334 BL02228 [1-27]putative hydrolase Molecular Function: hydrolase YpbG activity(GO:0016787) 2344 yocH [1-31] putative peptidogycan hydrolase“Biological Process: cell wall catabolism YocH (GO:0016998), BiologicalProcess: cell wall catabolism (GO:0016998)” 2347 BL00652 [1-32]hypothetical protein LytR 2348 yheN [1-53] “Carbohydrate Esterase Family“Biological Process: YheN 4, YheN” carbohydrate metabolism (GO:0005975),Molecular Function: hydrolase activity, acting on carbon-nitrogen (butnot peptide) bonds (GO:0016810)” 2349 [1-29] hypothetical protein 2360dacB [1-28] D-alanyl-D-alanine “required for spore cortex DacBcarboxypeptidase (penicillin- synthesis, Molecular Function: bindingprotein 5*) serine carboxypeptidase activity (GO:0004185), BiologicalProcess: proteolysis and peptidolysis (GO:0006508)” 2371 BL01892 [1-44]conserved hypothetical protein YpuD 2376 BL03279 [1-24] conservedhypothetical protein YpuA 2392 dacF [1-33] penicilin binding protein(putative “required for spore cortex DacF D-alanyl-D-alanine synthesis,Molecular Function: carboxypeptidase) serine carboxypeptidase activity(GO:0004185), Biological Process: proteolysis and peptidolysis(GO:0006508)” 2400 BL01161 [1-28] “Phosphotransferase system, “MolecularFunction: sugar porter activity lactose/cellobiose-specific IIB(GO:0005351), Biological Process: subunit” phosphoenolpyruvate-dependentsugar phosphotransferase system (GO:0009401)” 2418 lip [1-31] lipaseMolecular Function: catalytic Lip activity (GO:0003824) 2420 appAA[1-32] oligopeptide ABC transporter “oligopeptide transport, MolecularAppA (oligopeptide-binding protein) Function: transporter activity(GO:0005215), Biological Process: transport (GO:0006810)” 2435 [1-24]hypothetical protein 2436 [1-29] hypothetical protein 2438 BL00808[1-38] hypothetical protein YmaC 2449 [1-35] hypothetical protein 2450[1-31] conserved hyopothetical protein SsuA 2456 BL01380 [1-28]“Phosphotransferase system, “Molecular Function: sugar porter activitylactose/cellobiose-specific IIB (GO:0005351), Biological Process:subunit” phosphoenolpyruvate-dependent sugar phosphotransferase system(GO:0009401)” 2481 BL01514 [1-36] Cell wall hydrolase/autolysin“Molecular Function: N- YqiI acetylmuramoyl-L-alanine amidase activity(GO:0008745), Biological Process: peptidoglycan catabolism (GO:0009253)”2482 yqiH [1-33] YqiH YqiH 2484 [1-26] hypothetical protein 2499spoIIIAH [1-32] SpoIIIAH “mutants block sporulation after SpoIIIAHengulfment,” 2505 spoIIIAB [1-23] SpoIIIAB “mutants block sporulationafter SpoIIIAB engulfment,” 2517 yqhL [1-24] conserved protein YqhL YqhL2518 opuAC [1-31] glycine betaine ABC transporter “glycine betaine OpuAC(glycine betaine-binding protein) transport, Molecular Function:transporter activity (GO:0005215), Molecular Function: binding(GO:0005488), Biological Process: transport (GO:0006810)” 2528 tasA[1-28] translocation-dependent antimicrobial spore component TasA 2530yqxM [1-44] YqxM YqxM 2531 yqzG [1-24] YqzG YqzG 2533 comGG [1-38]probably part of the DNA “required for exogenous DNA- ComGG transportmachinery ComGG binding,” 2546 yqgU [1-28] conserved protein YqgU YqgU2548 fhuD [1-32] ferrichrome ABC transporter “Molecular Function: ironion FhuD (ferrichrome-binding protein) transporter activity(GO:0005381), Biological Process: high affinity iron ion transport(GO:0006827)” 2560 yqzC [1-33] conserved protein YqzC YqzC 2565 pstS[1-33] phosphate ABC transporter “involved in high-affinity PstS(binding protein) phosphate uptake, Molecular Function: transporteractivity (GO:0005215), Biological Process: transport (GO:0006810)” 2566pbpA [1-44] penicillin-binding protein 2A “involved in the synthesis ofPbpA peptidoglycan associated with cell wall elongation in sporeoutgrowth, Molecular Function: penicillin binding (GO:0008658)” 2571yqfZ [1-40] conserved protein YqfZ 2616 BL01411 [1-37] conservedhypothetical protein YqfA 2632 yqxA [1-29] YqxA YqxA 2633 spoIIP [1-55]SpoIIP “required for dissolution of the SpoIIP septal cell wall,” 2652BL02075 [1-36] putative lipoprotein Molecular Function: catalytic YqeFactivity (GO:0003824) 2697 appAB [1-36] oligopeptide ABC transporter“oligopeptide transport, Molecular AppA (oligopeptide-binding protein)Function: transporter activity (GO:0005215), Biological Process:transport (GO:0006810)” 2706 [1-32] putative phosphoesterase 2707BL02428 [1-27] carboxylesterase family Molecular Function: catalyticPnbA activity (GO:0003824) 2710 BL03118 [1-28] hypothetical protein YndA2712 sacC [1-24] Glycoside Hydrolase Family 32 “Molecular Function:hydrolase SacC activity, hydrolyzing O-glycosyl compounds (GO:0004553),Biological Process: carbohydrate metabolism (GO:0005975)” 2729 BL00468[1-33] Periplasmic iron-binding protein “Molecular Function: iron ionYxeB transporter activity (GO:0005381), Biological Process: highaffinity iron ion transport (GO:0006827)” 2745 yrrS [1-50] conservedprotein YrrS YrrS 2752 yrrL [1-44] conserved protein YrrL YrrL 2771 yrvJ[1-29] N-acetylmuramoyl-L-alanine amidase YrvJ YrvJ 2793 ymaC [1-24]phage-related protein YmaC YmaC 2797 coxA [1-27] spore cortex proteinCoxA 2801 nadB [1-37] L-aspartate oxidase “required for NADbiosynthesis,” NadB 2818 mreC [1-33] cell-shape determining protein “,Biological Process: regulation MreC of cell shape (GO:0008360)” 2866[1-28] hypothetical protein 2872 gerM [1-34] spore germination proteinGerM “germination (cortex hydrolysis) GerM and sporulation (stage II,multiple polar septa),” 2898 [1-33] hypothetical protein 2911 araN[1-32] sugar-binding protein “L-arabinose transport, Molecular AraNFunction: transporter activity (GO:0005215), Biological Process:transport (GO:0006810)” 2916 abnAB [1-28] Glycoside Hydrolase Family 43“degradation of plant cell wall AbnA polysaccharide, Molecular Function:hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553),Biological Process: carbohydrate metabolism (GO:0005975)” 2939 pelB[1-31] “pectate lyase, Polysaccharide Lyase Family 1” PelB 3005 ytxE[1-51] YtxE Cellular Component: outer YtxE membrane (sensu Gram-negative Bacteria) (GO:0009279) 3049 ytcQ [1-30] putative multiple sugartransport “Molecular Function: transporter YtcQ system substrate-bindingprotein activity (GO:0005215), Biological YtcQ Process: transport(GO:0006810)” 3059 BL00013 [1-30] putative lipoprotein YusA 3081 ytlA[1-33] putative sulfonate transport system substrate-binding proteinYtlA YtlA 3088 ytkA [1-29] conserved protein YtkA YtkA 3093 BL05310[1-29] Periplasmic solute binding “Molecular Function: binding YcdHprotein (GO:0005488), Cellular Component: periplasmic space (sensuGram-negative Bacteria) (GO:0030288)” 3217 [1-22] hypothetical proteinMolecular Function: catalytic activity (GO:0003824) 3235 yuiC [1-34]conserved protein YuiC YuiC 3264 yutC [1-24] conserved protein YutC YutC3266 BL02121 [1-22] putative metallopeptidase “Molecular Function: YunAmetalloendopeptidase activity (GO:0004222), Biological Process:proteolysis and peptidolysis (GO:0006508)” 3267 [1-32] hypotheticalprotein 3269 yunB [1-41] conserved protein YunB YunB 3284 yurYA [1-40]extracellular ribonuclease Molecular Function: nuclease YurI activity(GO:0004518) 3298 yusA [1-28] putative ABC transport systemsubstrate-binding protein YusA YusA 3318 yfiY [1-29] ABC transportsystem substrate- “Molecular Function: iron ion YfiY binding proteintransporter activity (GO:0005381), Biological Process: high affinityiron ion transport (GO:0006827)” 3319 yusW [1-28] YusW YusW 3333 gerAC[1-25] spore germination protein A3 “germination response to L- GerACprecursor alanine and related amino acids (earliest stage),” 3338 yvqG[1-39] conserved protein YvqG “Biological Process: metabolism YvqG(GO:0008152), Molecular Function: oxidoreductase activity (GO:0016491)”3349 yvrC [1-37] putative iron transport system “Molecular Function:iron ion YvrC substrate-binding protein YvrC transporter activity(GO:0005381), Biological Process: high affinity iron ion transport(GO:0006827)” 3380 BL02684 [1-27] putative Extracellular solute-Molecular Function: transporter YvfK binding protein activity(GO:0005215) 3384 BL02680 [1-31] putative Glycoside Hydrolase Family 3YbbD 3387 mntA [1-28] manganese transport system “Molecular Function:binding YcdH substrate-binding protein (GO:0005488), Cellular Component:periplasmic space (sensu Gram-negative Bacteria) (GO:0030288)” 3429[1-33] hypothetical protein 3465 [1-32] hypothetical protein 3492 opuCC[1-32] glycine betaine/carnitine/choline “high affinity transport ofglycine OpuCC ABC transporter betaine, carnitine, and(osmoprotectant-binding protein) choline, Molecular Function:transporter activity (GO:0005215), Molecular Function: binding(GO:0005488), Biological Process: transport (GO:0006810)” 3499 BL03470[1-29] “Periplasmic binding “Molecular Function: iron ion YfiY protein,putative iron transporter” transporter activity (GO:0005381), BiologicalProcess: high affinity iron ion transport (GO:0006827)” 3510 [1-26]hypothetical protein 3547 sacB [1-30] “levansucrase, Glycoside“Molecular Function: hydrolase SacB Hydrolase Family 68” activity,hydrolyzing O-glycosyl compounds (GO:0004553), Biological Process: sugarutilization (GO:0007587)” 3548 levB [1-33] “endolevanase, GlycosideHydrolase Family 32” YveB 3559 [1-29] putative ribonuclease “MolecularFunction: RNA binding (GO:0003723), Molecular Function: endoribonucleaseactivity (GO:0004521)” 3578 yvpB [1-39] putative cysteine protease YvpB“Molecular Function: cysteine- YvpB type endopeptidase activity(GO:0004197), Biological Process: proteolysis and peptidolysis(GO:0006508)” 3579 yvpA [1-29] Polysaccharide Lyase Family 3 YvpA 3587yvnB [1-29] YvnB YvnB 3600 ctpB [1-37] “Peptidase S41A, C-terminal“Biological Process: proteolysis YvjB protease” and peptidolysis(GO:0006508), Molecular Function: serine-type peptidase activity(GO:0008236) 3602 BL03388 [1-29] “Spectrin repeat, Rudiment single “,”YvcE hybrid motif” 3643 lytC [1-14] N-acetylmuramoyl-L-alanine “involvedin cell separation, cell LytC amidase (major autolysin) wall turnover,antibiotic-induced (CWBP49) lysis, motility and general cell lysisinduced by sodium azide, Molecular Function: N- acetylmuramoyl-L-alanineamidase activity (GO:0008745), Biological Process: peptidoglycancatabolism 3644 lytB [1-26] modifier protein of major autolysin LytC(CWBP76) LytB 3645 BL03297 [1-25] membrane bound lipoprotein involved inthe secretion of LytA major autolysin 3646 lytR [1-32] membrane-boundprotein “attenuator role for lytABC and LytR lytR expression,” 3656 lytD[1-29] “N-acetylglucosaminidase (major “involved in cell separation,cell LytD autolysin), Glycoside Hydrolase wall turnover,antibiotic-induced Family 73” lysis and motility, Molecular Function:amidase activity (GO:0004040), Biological Process: peptidoglycancatabolism (GO:0009253)” 3670 pgdS [1-34] gamma-DL-glutamyl hydrolase“gamma-DL-glutamyl hydrolase YwtD cleaving the gamma-glutamyl bond onlybetween D- and L- glutamic acids of PGA, DL- endopeptidase II family”3671 ywtC [1-25] YwtC 3672 pgsAA [1-47] poly-gamma-glutamate synthesisprotein YwtB 3680 rbsB [1-28] ribose ABC transporter (ribose- “ribosetransport,” RbsB binding protein) 3738 feuA [1-28] iron-binding protein“component of iron-uptake FeuA system, Molecular Function: iron iontransporter activity (GO:0005381), Biological Process: high affinityiron ion transport (GO:0006827)” 3751 ywmD [1-25] YwmD YwmD 3752 ywmC[1-24] YwmC YwmC 3753 spoIID [1-33] SpoIID “required for complete SpoIIDdissolution of the asymmetric septum,” 3755 ywmB [1-30] conservedprotein YwmB YwmB 3773 spoIIR [1-29] SpoIIR required for processing ofpro- SpoIIR sigma-E 3801 ywjE [1-28] putative Phospholipase “MolecularFunction: catalytic YwjE activity (GO:0003824), Biological Process:metabolism (GO:0008152)” 3808 BL03962 [1-41] “Penicillin-bindingprotein, “Molecular Function: penicillin YwhE Glycosyl TransferaseFamily 51” binding (GO:0008658), Biological Process: cell wallbiosynthesis (sensu Bacteria) (GO:0009273)” 3812 [1-25] hypotheticalprotein 3829 BL03904 [1-32] hypothetical protein YuaB 3849 vpr [1-29]extracellular serine protease “Molecular Function: subtilase Vpractivity (GO:0004289), Biological Process: proteolysis and peptidolysis(GO:0006508)” 3906 ywaD [1-31] Putative aminopeptidase “BiologicalProcess: proteolysis YwaD and peptidolysis (GO:0006508), MolecularFunction: peptidase activity (GO:0008233)” 3914 dltD [1-27] precusorDltD D-alanine esterification of DltD lipoteichoic acid and wallteichoic acid (D-alanine transfer from undecaprenol-P to thepoly(glycerophosphate) chain of LTA) 3920 licB [1-24] phosphotransferasesystem “Molecular Function: sugar porter activity (PTS)lichenan-specific enzyme (GO:0005351), Biological Process: IIB componentphosphoenolpyruvate-dependent sugar phosphotransferase system(GO:0009401)” 3933 BL05372 [1-31] hypothetical protein YweA 3954 lanP[1-25] serine protease “subtilase activity IspA (GO:0004289),proteolysis and peptidolysis (GO:0006508), lantibiotic leader peptideprocessing” 3970 ansA [1-29] L-asparaginase “Molecular Function: AnsAasparaginase activity (GO:0004067), Biological Process: amino acidmetabolism (GO:0006520)” 3977 BL02958 [1-25] conserved hypotheticalprotein YxeA 3978 yxkH [1-28] “Polysaccharide deacetylase, “BiologicalProcess: YxkH Carbohydrate Esterase Family carbohydrate metabolism 4”(GO:0005975), Molecular Function: hydrolase activity, acting oncarbon-nitrogen (but not peptide) bonds (GO:0016810)” 3982 BL02963[1-30] Glycerophosphoryl diester “Biological Process: glycerol YhdWphosphodiesterase metabolism (GO:0006071), Molecular Function:glycerophosphodiester phosphodiesterase activity (GO:0008889)” 3983BL05379 [1-28] hypothetical protein 3996 [1-73] hypothetical protein4011 BL05383 [1-34] hypothetical protein YjeA 4016 [1-31] hypotheticalprotein Biological Process: electron transport (GO:0006118) 4046 yxiA[1-27] “Glycoside hydrolase, family 43” “Molecular Function: hydrolaseYxiA activity, hydrolyzing O-glycosyl compounds (GO:0004553), BiologicalProcess: carbohydrate metabolism (GO:0005975)” 4058 ydaJ [1-29] putativeGlycoside transferase YdaJ 4080 glpQ [1-29] glycerophosphoryl diester“hydrolysis of deacylated GlpQ phosphodiesterase phospholipids,Biological Process: glycerol metabolism (GO:0006071), MolecularFunction: glycerophosphodiester phosphodiesterase activity (GO:0008889)”4099 yxeA [1-34] conserved hypothetical protein 4103 yvfO [1-28]Glyccosyl Hydrolase Family 53 YvfO 4107 cycB [1-32] “putativeextracellular solute- “Molecular Function: transporter YvfK bindingprotein, family 1 CycB” activity (GO:0005215), Biological Process:transport (GO:0006810)” 4121 [1-28] hypothetical protein 4128 [1-34]hypothetical protein 4130 [1-42] hypothetical protein 4158 yycH [1-36]conserved hypothetical YycH YycH

TABLE 4 Codon Usage Table for Chromosome Amino # Codon acid Fract /1000Number GCA A 0.230 18.394 22495 GCC A 0.272 21.724 26567 GCG A 0.30124.082 29451 GCT A 0.197 15.766 19281 TGC C 0.662 5.113 6253 TGT C 0.3382.613 3195 GAC D 0.432 22.129 27063 GAT D 0.568 29.114 35605 GAA E 0.70451.721 63253 GAG E 0.296 21.797 26657 TTC F 0.354 16.034 19609 TTT F0.646 29.223 35738 GGA G 0.316 22.644 27693 GGC G 0.374 26.825 32806 GGGG 0.171 12.287 15026 GGT G 0.139 9.957 12177 CAC H 0.386 8.412 10288 CATH 0.614 13.369 16350 ATA I 0.101 7.217 8826 ATC I 0.487 34.741 42487 ATTI 0.412 29.376 35926 AAA K 0.693 49.125 60078 AAG K 0.307 21.739 26586CTA L 0.032 3.106 3798 CTC L 0.152 14.848 18158 CTG L 0.263 25.592 31298CTT L 0.229 22.330 27309 TTA L 0.143 13.920 17023 TTG L 0.181 17.63921572 ATG M 1.000 26.541 32458 AAC N 0.505 19.159 23431 AAT N 0.49518.780 22967 CCA P 0.112 4.189 CCC P 0.120 4.477 5475 CCG P 0.520 19.40523731 CCT P 0.248 9.236 11295 CAA Q 0.465 16.656 20369 CAG Q 0.53519.179 23455 AGA R 0.218 9.611 11754 AGG R 0.146 6.448 7886 CGA R 0.0743.275 4005 CGC R 0.253 11.173 13664 CGG R 0.194 8.538 10441 CGT R 0.1145.044 6168 AGC S 0.263 15.893 19436 AGT S 0.063 3.820 4672 TCA S 0.20212.173 14887 TCC S 0.154 9.287 11357 TCG S 0.171 10.289 12583 TCT S0.147 8.865 10841 ACA T 0.327 16.969 20752 ACC T 0.188 9.765 11942 ACG T0.373 19.404 23730 ACT T 0.112 5.833 7134 GTA V 0.144 9.710 11875 GTC V0.361 24.342 29769 GTG V 0.238 16.042 19619 GTT V 0.257 17.354 21223 TGGW 1.000 10.303 12600 TAC Y 0.398 13.468 16471 TAT Y 0.602 20.348 24885TAA * 0.551 1.962 2400 TAG * 0.157 0.558 682 TGA * 0.292 1.041 1273

TABLE 5 Signal Peptide Codon Usage Table Amino Codon acid Fract /1000Number GCA A 0.294 42.315 106 GCC A 0.219 31.537 79 GCG A 0.242 34.73187 GCT A 0.244 35.13 88 TGC C 0.543 9.98 25 TGT C 0.457 8.383 21 GAC D0.231 2.395 6 GAT D 0.769 7.984 20 GAA E 0.784 15.968 40 GAG E 0.2164.391 11 TTC F 0.335 21.956 55 TTT F 0.665 43.513 109 GGA G 0.341 22.35556 GGC G 0.262 17.166 43 GGG G 0.183 11.976 30 GGT G 0.213 13.972 35 CACH 0.316 2.395 6 CAT H 0.684 5.19 13 ATA I 0.142 9.98 25 ATC I 0.46632.735 82 ATT I 0.392 27.545 69 AAA K 0.767 61.876 155 AAG K 0.23318.762 47 CTA L 0.05 7.186 18 CTC L 0.098 13.972 35 CTG L 0.235 33.53384 CTT L 0.16 22.754 57 TTA L 0.221 31.537 79 TTG L 0.235 33.533 84 ATGM 1 51.497 129 AAC N 0.482 10.778 27 AAT N 0.518 11.577 29 CCA P 0.1644.79 12 CCC P 0.192 5.589 14 CCG P 0.452 13.174 33 CCT P 0.192 5.589 14CAA Q 0.475 11.577 29 CAG Q 0.525 12.774 32 AGA R 0.333 9.98 25 AGG R0.08 2.395 6 CGA R 0.107 3.194 8 CGC R 0.16 4.79 12 CGG R 0.173 5.19 13CGT R 0.147 4.391 11 AGC S 0.171 14.77 37 AGT S 0.093 7.984 20 TCA S0.241 20.758 52 TCC S 0.148 12.774 32 TCG S 0.134 11.577 29 TCT S 0.21318.363 46 ACA T 0.262 11.178 28 ACC T 0.234 9.98 25 ACG T 0.327 13.97235 ACT T 0.178 7.585 19 GTA V 0.216 14.371 36 GTC V 0.186 12.375 31 GTGV 0.335 22.355 56 GTT V 0.263 17.565 44 TGG W 1 7.585 19 TAC Y 0.4055.988 15 TAT Y 0.595 8.782 22 TAA * 0 0 0 TAG * 0 0 0 TGA * 0 0 0

Example 6 Identification of Genes Induced by Glucose Limitation UsingDNA Microarrays

Bacillus licheniformis ATCC 14580 was grown in duplicate shake flaskscontaining 100 ml of Spizizen I medium (Anagnostopoulos and Spizizen,1961, Journal of Bacteriology 81: 741-748). The inocula for the shakeflasks were obtained from 10 ml of mid-log cells (approximately 80-90Klett units) and a 0 hour total RNA sample was extracted from 30 ml ofthe mid-log cells, as described below. Total cellular RNA was alsopurified from 10 ml of the Spizizen I shake flasks that were sampled at2, 4, 6 and 8 hours post inoculum. All of the cell samples were mixedwith two volumes of RNAProtect RNA stabilizing reagent (QIAGEN, Inc.,Valencia, Calif.) for 5 minutes. The cells were subsequently pelleted at2800×g, 4° C. for 10 minutes, the supernatant was discarded, the cellpellets were frozen at −80° C., and cellular RNA was purified using aFastRNA Pro Blue kit (QBiogene, Carlsbad, Calif.) using the protocolsupplied by the manufacturer. The frozen cell pellets from each samplewere resuspended in 2 ml RNAPro solution provided in the FastRNA ProBlue kit and RNA was obtained from two lysis matrix B vials.

Sixteen replicate cDNA targets for the 0 time point and two technicalreplicate cDNA targets for each of the rest of the time points wereprepared and hybridized to Bacillus licheniformis DNA microarraysprepared as described by Berka et al., 2002, Molecular Microbiology 43:1331-1345. Dye swap hybridizations using the zero time point asreference were prepared as described in Hu et al. [In G. Parmigiani, E.S. Garrett, R. A Irizarry, and S. L Zeger (eds.) The Analysis of GeneExpression Data, pp. 318-319, Springer-Verlag, New York (2003)].

The arrays were scanned with an Axon 4000B scanner and formatted foranalysis with GenePix Pro version 5.0 (Axon Instruments, Inc, RedwoodCity, Calif.). The fluorescence intensity data from GenePix was importeddirectly into S-Plus ArrayAnalyzer version 2 software (InsightfulCorporation, Seattle, Wash.) for statistical analysis. The raw intensitydata were normalized using the lowess (locally weighted regression)function provided in S-Plus ArrayAnalyzer software, and the genes thatwere differentially expressed at each time point relative to the timezero reference were identified by employing a multiple comparison t-testwith Bonferroni correction as outlined in the user's guide to S-PlusArrayAnalyzer software (Insightful Corp., Seattle, Wash.). Thefamily-wise error rate (FWER) was set at 0.1. The list of genes at eachtime point that passed these statistical criteria were used to query apathway-genome database established using Pathway Tools Suite software(Karp et al., 2002, Bioinformatics 18 suppl. 1: S225-S232), and the geneexpression profiles were painted onto a schematic of metabolic pathways.In doing so, those pathways that were significantly altered at each timepoint were identified.

As a result of this analysis the following observations were made: (1)Early in the cultures, genes encoding enzymes of the glycolytic pathwaywere slightly induced reflecting the change from partially depletedglucose levels in the inoculum culture to ample glucose in the shakeflask medium; (2) As glucose was progressively depleted in the shakeflask culture (times 6 and 8 hours), genes encoding glycolytic pathwayenzymes were down-regulated; (3) genes that encode enzymes forphenylalanine, tryptophan, histidine, and arginine biosynthetic pathwayswere up-regulated during stationary phase suggesting that cells hadexhausted critical amino acids, and they were turning on the genes forpathways to synthesize more of these essential nutrients; (4) as cellsentered stationary phase, cell division, DNA replication, and ribosomeproduction all decreased to a low level. This was reflected in theshut-down of purine and pyrimidine biosynthetic pathways anddown-regulation of genes encoding many ribosomal proteins.

The invention described and claimed herein is not to be limited in scopeby the specific aspects herein disclosed, since these aspects areintended as illustrations of several aspects of the invention. Anyequivalent aspects are intended to be within the scope of thisinvention. Indeed, various modifications of the invention in addition tothose shown and described herein will become apparent to those skilledin the art from the foregoing description. Such modifications are alsointended to fall within the scope of the appended claims. In the case ofconflict, the present disclosure including definitions will control.

Various references are cited herein, the disclosures of which areincorporated by reference in their entireties.

1. An isolated gene which encodes a polypeptide having aspartate1-decarboxylase activity, selected from the group consisting of: (a) agene comprising a nucleotide sequence having at least 90% sequenceidentity with the nucleotide sequence of SEQ ID NO: 2275; and (b) a genecomprising a nucleotide sequence which hybridizes under high stringencyconditions with the nucleotide sequence of SEQ ID NO: 2275, or thecomplementary strand thereof, wherein high stringency conditions aredefined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3%SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50%formamide, and washing three times each for 15 minutes using 2×SSC, 0.2%SDS at 65° C.
 2. The isolated gene of claim 1, which comprises anucleotide sequence having at least 90% sequence identity with SEQ IDNO:
 2275. 3. The isolated gene of claim 1, which comprises a nucleotidesequence having at least 95% sequence identity with SEQ ID NO:
 2275. 4.The isolated gene of claim 1, which comprises a nucleotide sequencehaving at least 97% sequence identity with SEQ ID NO:
 2275. 5. Theisolated gene of claim 1, which comprises the nucleotide sequence of SEQID NO:
 2275. 6. The isolated gene of claim 1, which hybridizes underhigh stringency conditions with the nucleotide sequence of SEQ ID NO:2275, or the complementary strand thereof, wherein high stringencyconditions are defined as prehybridization and hybridization at 42° C.in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA,and 50% formamide, and washing three times each for 15 minutes using2×SSC, 0.2% SDS at 65° C.
 7. The isolated gene of claim 1, whichhybridizes under very high stringency conditions with the nucleotidesequence of SEQ ID NO: 2275, or the complementary strand thereof,wherein very high stringency conditions are defined as prehybridizationand hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared anddenatured salmon sperm DNA, and 50% formamide, and washing three timeseach for 15 minutes using 2×SSC, 0.2% SDS at 70° C.
 8. The isolated geneof claim 1, which is contained in Bacillus licheniformis ATCC
 14580. 9.A nucleic acid construct comprising the isolated gene of claim 1operably linked to one or more control sequences that direct theproduction of the polypeptide in a host cell.
 10. A recombinantexpression vector comprising the nucleic acid construct of claim
 9. 11.A recombinant host cell comprising the nucleic acid construct of claim9.
 12. An isolated gene which encodes a polypeptide having aspartate1-decarboxylase activity, wherein the polypeptide has at least 90%sequence identity with the amino acid sequence of SEQ ID NO:
 6472. 13.The isolated gene of claim 12, wherein the polypeptide has at least 95%sequence identity with the amino acid sequence of SEQ ID NO:
 6472. 14.The isolated gene of claim 12, wherein the polypeptide has at least 97%sequence identity with the amino acid sequence of SEQ ID NO:
 6472. 15.The isolated gene of claim 12, wherein the polypeptide comprises theamino acid sequence of SEQ ID NO:
 6472. 16. A nucleic acid constructcomprising the isolated gene of claim 12 operably linked to one or morecontrol sequences that direct the production of the polypeptide in anexpression host.
 17. A recombinant expression vector comprising thenucleic acid construct of claim
 16. 18. A recombinant host cellcomprising the nucleic acid construct of claim
 16. 19. A recombinanthost cell comprising a polynucleotide which encodes a polypeptide havingaspartate 1-decarboxylase activity and at least 90% sequence identitywith the amino acid sequence of SEQ ID NO: 6472, wherein thepolynucleotide is operably linked to a promoter heterologous to the hostcell.
 20. The recombinant host cell claim 19, wherein the polypeptidehas at least 95% sequence identity with the amino acid sequence of SEQID NO: 6472.